BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0082.Seq (627 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 93 3e-20 SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces ... 63 3e-11 SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosac... 27 1.7 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 1.7 SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S... 27 1.7 SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity prote... 27 1.7 SPBC30D10.06 |lsm4||U6 snRNP-associated protein Lsm4|Schizosacch... 27 2.9 SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 26 5.1 SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosacch... 25 6.8 SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 25 6.8 SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 9.0 >SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 437 Score = 93.1 bits (221), Expect = 3e-20 Identities = 69/209 (33%), Positives = 98/209 (46%), Gaps = 1/209 (0%) Frame = +2 Query: 2 GKPFVLPSVRKAEEILHSRGLNHEYAPISGEATYTDAVAKLAFGEDSPVIKNRSNCTVQT 181 GKP+VLPSVR+AE L S+ L+ EYAPI+G ++ KLA+G+ IK+R + Q+ Sbjct: 79 GKPYVLPSVRQAETELLSQKLDKEYAPITGIPSFRVQATKLAYGDVYESIKDRL-VSAQS 137 Query: 182 LSGTGALRLGLEFITKHYAKAKEICSRRLLGETTRK-SATPSTCRTRNTVTSIPRPMVSI 358 +SGTGAL + F+ Y S G S T ++ R + I Sbjct: 138 ISGTGALCIAANFLASFYPSKTIYVSDPTWGNHKNVFSRAGLTVKSYKYYDPATRGL-DI 196 Query: 359 CREHSRIYQKFPKFHYSVARVRTQPHRC*PQAQRLGTTFQGDQRKEIIPVFDMAYQGFAN 538 S + + P P + + Q+K + DMAYQGFA+ Sbjct: 197 KGMLSDLTSAPDGSIILLHACAHNPTGVDPTKAQWDDILKTMQKKNHFALLDMAYQGFAS 256 Query: 539 GDVDNDAFAVRLFVKEGHQVMLAQNFAKN 625 GD DA+A RLF ++L Q+FAKN Sbjct: 257 GDFARDAYATRLFASSNVPMLLCQSFAKN 285 Score = 57.2 bits (132), Expect = 2e-09 Identities = 24/79 (30%), Positives = 41/79 (51%) Frame = +1 Query: 265 PTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDISKIPEVPLFCCTRAHTTPPVL 444 PTWGNH + + L K Y+Y+DP T G D++G L D++ P+ + P + Sbjct: 165 PTWGNHKNVFSRAGLTVKSYKYYDPATRGLDIKGMLSDLTSAPDGSIILLHACAHNPTGV 224 Query: 445 TPSPATGNNFPR*SKKGNY 501 P+ A ++ + +K N+ Sbjct: 225 DPTKAQWDDILKTMQKKNH 243 Score = 52.0 bits (119), Expect = 7e-08 Identities = 20/35 (57%), Positives = 27/35 (77%) Frame = +3 Query: 399 SIILLHACAHNPTGVDPKPSDWEQLSKVIKERKLF 503 SIILLHACAHNPTGVDP + W+ + K ++++ F Sbjct: 210 SIILLHACAHNPTGVDPTKAQWDDILKTMQKKNHF 244 >SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 63.3 bits (147), Expect = 3e-11 Identities = 31/81 (38%), Positives = 49/81 (60%), Gaps = 1/81 (1%) Frame = +2 Query: 2 GKPFVLPSVRKAEEILHSRG-LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNRSNCTVQ 178 GKP++LP+V+KA +I+ + NHEY PI+G +T A A++ F + ++ ++Q Sbjct: 45 GKPWILPAVKKASKIVEEQASFNHEYLPIAGLPRFTKAAAEVLFRPNPHLLSEDRVASMQ 104 Query: 179 TLSGTGALRLGLEFITKHYAK 241 ++SGTGA L FI Y K Sbjct: 105 SVSGTGANFLAASFIETFYVK 125 Score = 51.2 bits (117), Expect = 1e-07 Identities = 23/46 (50%), Positives = 34/46 (73%) Frame = +2 Query: 488 RKEIIPVFDMAYQGFANGDVDNDAFAVRLFVKEGHQVMLAQNFAKN 625 RK ++ VFD+AYQGFA+GD++ D++A+ FVK + Q+FAKN Sbjct: 212 RKHLV-VFDIAYQGFASGDLNRDSWALNEFVKYNKDFFVCQSFAKN 256 Score = 46.8 bits (106), Expect = 3e-06 Identities = 21/58 (36%), Positives = 30/58 (51%) Frame = +3 Query: 324 PLLRSQDQWFRFAGSTRGYIKNSRSSIILLHACAHNPTGVDPKPSDWEQLSKVIKERK 497 P ++++ F + G SI LLHACAHNPTG+DP W + + + RK Sbjct: 156 PYWDAKNRSFDYEGMLSTIKSAPEGSIFLLHACAHNPTGIDPTREQWLSIFESLLSRK 213 Score = 39.5 bits (88), Expect = 4e-04 Identities = 19/66 (28%), Positives = 27/66 (40%) Frame = +1 Query: 256 LPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDISKIPEVPLFCCTRAHTTP 435 + PTW H + L + Y Y+D K FD +G L I PE +F P Sbjct: 133 ISNPTWPVHRTLWEKLGVTVDTYPYWDAKNRSFDYEGMLSTIKSAPEGSIFLLHACAHNP 192 Query: 436 PVLTPS 453 + P+ Sbjct: 193 TGIDPT 198 >SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 27.5 bits (58), Expect = 1.7 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Frame = +3 Query: 375 GYIKNSRSSIILLHACAHNPTG-VDPKPSDWEQLSKVIKERKLFPF 509 G+I + +++L AC N T VD +P D E+ ++I+ +F F Sbjct: 13 GFIGTTVDALLLFEACRQNYTHFVDRRPQDRER-ERLIRSGSVFVF 57 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.5 bits (58), Expect = 1.7 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 2/33 (6%) Frame = -2 Query: 455 GLGVNTGGVVCARVQQNN--GTSGIFDISSSAP 363 G+ N G V R + + GTSG +D+SS++P Sbjct: 423 GMSNNFGSVPLGRTESSPAWGTSGYYDVSSTSP 455 >SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|Schizosaccharomyces pombe|chr 1|||Manual Length = 512 Score = 27.5 bits (58), Expect = 1.7 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = -3 Query: 469 CSQSLGLGSTPVGLCAHACNRIMELREF 386 C SLG+GS G+C A + +L F Sbjct: 406 CQDSLGMGSCASGVCTSASRQCKKLTNF 433 >SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity protein kinase Mph1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 678 Score = 27.5 bits (58), Expect = 1.7 Identities = 15/42 (35%), Positives = 20/42 (47%) Frame = +1 Query: 337 PKTNGFDLQGALEDISKIPEVPLFCCTRAHTTPPVLTPSPAT 462 P+T FDLQG S + EV H ++TP P+T Sbjct: 76 PRTKNFDLQGNGGQNSALKEVNTPAYQSMHHFEHLITPLPST 117 >SPBC30D10.06 |lsm4||U6 snRNP-associated protein Lsm4|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 26.6 bits (56), Expect = 2.9 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = -3 Query: 571 TDCESVIVDITVGETLIRHVENGNNFLSLITLE 473 T ++V++ GET H+EN +N+++L E Sbjct: 10 TQGRPILVELKNGETFNGHLENCDNYMNLTLRE 42 >SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 25.8 bits (54), Expect = 5.1 Identities = 9/13 (69%), Positives = 10/13 (76%) Frame = +1 Query: 250 DLLPTPTWGNHPQ 288 D+LPT TWG PQ Sbjct: 295 DVLPTVTWGTSPQ 307 >SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosaccharomyces pombe|chr 1|||Manual Length = 1030 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/47 (27%), Positives = 21/47 (44%) Frame = +2 Query: 317 RNTVTSIPRPMVSICREHSRIYQKFPKFHYSVARVRTQPHRC*PQAQ 457 +N TSI P + +E + + K+ + +V T P C P Q Sbjct: 593 KNEETSINIPNETAVKEFHDLKLQLEKYGEEIQKVMTHPDNCLPYLQ 639 >SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 1563 Score = 25.4 bits (53), Expect = 6.8 Identities = 14/49 (28%), Positives = 21/49 (42%) Frame = +1 Query: 199 TPTRTRVHNETLR*GKGDLLPTPTWGNHPQICNTLNLPHKKYRYFDPKT 345 TP+ + T G L T T + P + NT+ +P+ Y P T Sbjct: 744 TPSVSATTTTTTIGYSGSLTTTLTGSSGPVVTNTVEIPYGNSSYIIPTT 792 >SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 25.0 bits (52), Expect = 9.0 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -1 Query: 228 FVMNSSPSRSAPVPDNVCTVQLLLFLMTGL 139 F NSSP+RS P + ++LLL T + Sbjct: 208 FHRNSSPNRSLPRSRKIDLLKLLLIFSTSI 237 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,833,613 Number of Sequences: 5004 Number of extensions: 63821 Number of successful extensions: 191 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 174 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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