BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0074.Seq (735 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP22H7.06 |||nicotinamide riboside kinase |Schizosaccharomyces... 29 0.69 SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 3.7 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 27 3.7 SPAC630.09c |mug58||glycerate kinase |Schizosaccharomyces pombe|... 27 3.7 SPAC30.02c |||RNA polymerase II elongator complex subunit|Schizo... 27 3.7 SPAC4G8.10 |gos1||SNARE Gos1|Schizosaccharomyces pombe|chr 1|||M... 26 4.8 SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 26 6.4 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 26 6.4 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 25 8.5 >SPBP22H7.06 |||nicotinamide riboside kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 29.1 bits (62), Expect = 0.69 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = +3 Query: 639 VIIGISGVTCGGKTTLANKL 698 +I+G+SG +C GK+TL L Sbjct: 6 IIVGVSGASCSGKSTLCQLL 25 >SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 26.6 bits (56), Expect = 3.7 Identities = 12/22 (54%), Positives = 17/22 (77%), Gaps = 1/22 (4%) Frame = +3 Query: 546 SHKK-SIQYRTTNFVPPSANLS 608 SH+K + YRTT+F+P S +LS Sbjct: 240 SHEKLDLPYRTTDFIPYSKDLS 261 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 26.6 bits (56), Expect = 3.7 Identities = 18/60 (30%), Positives = 29/60 (48%) Frame = +3 Query: 540 DLSHKKSIQYRTTNFVPPSANLSQNSNMPGKDWVIIGISGVTCGGKTTLANKLKNALTPV 719 DLS ++ + N V PS NLS NS+ + V+ + + + +NALTP+ Sbjct: 144 DLSINRNTNKKALNAVSPSLNLSSNSS----------VQDVSIDKEEMMKKQSRNALTPL 193 >SPAC630.09c |mug58||glycerate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 26.6 bits (56), Expect = 3.7 Identities = 13/24 (54%), Positives = 16/24 (66%) Frame = +3 Query: 642 IIGISGVTCGGKTTLANKLKNALT 713 I+GISG GK+TLA+ L LT Sbjct: 27 ILGISGPQGSGKSTLASALDTELT 50 >SPAC30.02c |||RNA polymerase II elongator complex subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 281 Score = 26.6 bits (56), Expect = 3.7 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = +3 Query: 642 IIGISGVTCGGKTTLANKLKNAL 710 +I +SG GKTT +N+LK AL Sbjct: 3 LIIVSGYPSSGKTTRSNELKKAL 25 >SPAC4G8.10 |gos1||SNARE Gos1|Schizosaccharomyces pombe|chr 1|||Manual Length = 182 Score = 26.2 bits (55), Expect = 4.8 Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 6/61 (9%) Frame = +3 Query: 270 KKLLVLSEPAADPN------IVEKIIQRAKKQKPLLENTEVKKGKEKSILFPEEKQSFQD 431 +KLL + +AD N ++ +R K+ + LL K ++ +L PEE + Q+ Sbjct: 35 RKLLEEANSSADENDSHSMATIKSHFERLKRDEQLLNGVLKKYDAKQEVLSPEELRDAQN 94 Query: 432 F 434 F Sbjct: 95 F 95 >SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 25.8 bits (54), Expect = 6.4 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = +3 Query: 258 DQNIKKLLVLSEPAADPNIVEKIIQRAKKQKPLL 359 D N+K+ E ++ N+ E +++R KK P+L Sbjct: 385 DHNVKRYGKSEESSSTMNVGEGLLKRTKKYIPIL 418 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.8 bits (54), Expect = 6.4 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +3 Query: 519 PHLPLFIDLSHKKSIQYRTTNFVPPSANLSQNS 617 P+LPL D S K ++Y NF+ N + S Sbjct: 253 PNLPLLYDKSSGKRVEYSAFNFLALYENGEERS 285 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.4 bits (53), Expect = 8.5 Identities = 13/53 (24%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Frame = -3 Query: 316 TILGSAAGSERTSNFFIFWSTYYH*LCCSFFHF--YFCMDEELLDLQTSNVSY 164 T+LG + FF+ +S+ + +C SFF + C+++ + ++ +N+ Y Sbjct: 126 TLLGLPLARQVFRQFFVLFSSDFLMICLSFFSYGLQVCIEKNM--IRWANLGY 176 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,993,571 Number of Sequences: 5004 Number of extensions: 61292 Number of successful extensions: 156 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 156 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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