BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0074.Seq
(735 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP22H7.06 |||nicotinamide riboside kinase |Schizosaccharomyces... 29 0.69
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 3.7
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 27 3.7
SPAC630.09c |mug58||glycerate kinase |Schizosaccharomyces pombe|... 27 3.7
SPAC30.02c |||RNA polymerase II elongator complex subunit|Schizo... 27 3.7
SPAC4G8.10 |gos1||SNARE Gos1|Schizosaccharomyces pombe|chr 1|||M... 26 4.8
SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 26 6.4
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 26 6.4
SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 25 8.5
>SPBP22H7.06 |||nicotinamide riboside kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 29.1 bits (62), Expect = 0.69
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3
Query: 639 VIIGISGVTCGGKTTLANKL 698
+I+G+SG +C GK+TL L
Sbjct: 6 IIVGVSGASCSGKSTLCQLL 25
>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 26.6 bits (56), Expect = 3.7
Identities = 12/22 (54%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +3
Query: 546 SHKK-SIQYRTTNFVPPSANLS 608
SH+K + YRTT+F+P S +LS
Sbjct: 240 SHEKLDLPYRTTDFIPYSKDLS 261
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 26.6 bits (56), Expect = 3.7
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = +3
Query: 540 DLSHKKSIQYRTTNFVPPSANLSQNSNMPGKDWVIIGISGVTCGGKTTLANKLKNALTPV 719
DLS ++ + N V PS NLS NS+ + V+ + + + +NALTP+
Sbjct: 144 DLSINRNTNKKALNAVSPSLNLSSNSS----------VQDVSIDKEEMMKKQSRNALTPL 193
>SPAC630.09c |mug58||glycerate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 277
Score = 26.6 bits (56), Expect = 3.7
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +3
Query: 642 IIGISGVTCGGKTTLANKLKNALT 713
I+GISG GK+TLA+ L LT
Sbjct: 27 ILGISGPQGSGKSTLASALDTELT 50
>SPAC30.02c |||RNA polymerase II elongator complex
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 281
Score = 26.6 bits (56), Expect = 3.7
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +3
Query: 642 IIGISGVTCGGKTTLANKLKNAL 710
+I +SG GKTT +N+LK AL
Sbjct: 3 LIIVSGYPSSGKTTRSNELKKAL 25
>SPAC4G8.10 |gos1||SNARE Gos1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 182
Score = 26.2 bits (55), Expect = 4.8
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 6/61 (9%)
Frame = +3
Query: 270 KKLLVLSEPAADPN------IVEKIIQRAKKQKPLLENTEVKKGKEKSILFPEEKQSFQD 431
+KLL + +AD N ++ +R K+ + LL K ++ +L PEE + Q+
Sbjct: 35 RKLLEEANSSADENDSHSMATIKSHFERLKRDEQLLNGVLKKYDAKQEVLSPEELRDAQN 94
Query: 432 F 434
F
Sbjct: 95 F 95
>SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 25.8 bits (54), Expect = 6.4
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 258 DQNIKKLLVLSEPAADPNIVEKIIQRAKKQKPLL 359
D N+K+ E ++ N+ E +++R KK P+L
Sbjct: 385 DHNVKRYGKSEESSSTMNVGEGLLKRTKKYIPIL 418
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 519 PHLPLFIDLSHKKSIQYRTTNFVPPSANLSQNS 617
P+LPL D S K ++Y NF+ N + S
Sbjct: 253 PNLPLLYDKSSGKRVEYSAFNFLALYENGEERS 285
>SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 537
Score = 25.4 bits (53), Expect = 8.5
Identities = 13/53 (24%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
Frame = -3
Query: 316 TILGSAAGSERTSNFFIFWSTYYH*LCCSFFHF--YFCMDEELLDLQTSNVSY 164
T+LG + FF+ +S+ + +C SFF + C+++ + ++ +N+ Y
Sbjct: 126 TLLGLPLARQVFRQFFVLFSSDFLMICLSFFSYGLQVCIEKNM--IRWANLGY 176
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,993,571
Number of Sequences: 5004
Number of extensions: 61292
Number of successful extensions: 156
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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