Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0065.Seq
(789 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 113 4e-26
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 113 4e-26
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 48 2e-06
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 46 8e-06
SPAC24C9.11 |||MIF4G/MA4 domain protein|Schizosaccharomyces pomb... 28 1.8
SPBC3B9.21 |dcp1||mRNA decapping complex subunit Dcp1|Schizosacc... 27 2.3
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 27 2.3
SPBC1198.03c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 4.0
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 26 5.4
SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyce... 26 7.1
SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase |Schizosaccha... 26 7.1
SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr 1|... 25 9.4
SPCC1393.06c |||rRNA processing protein Ipi1|Schizosaccharomyces... 25 9.4
SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces p... 25 9.4
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 25 9.4
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 25 9.4
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 113 bits (271), Expect = 4e-26
Identities = 54/92 (58%), Positives = 64/92 (69%)
Frame = +3
Query: 510 QMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFY 689
+MI +HLPSP AQ+YR E LYEGP DDE A+GI++CD APLM+YVSKMVPTSD+GRFY
Sbjct: 336 EMIVLHLPSPKTAQQYRAETLYEGPMDDECAVGIRNCDANAPLMIYVSKMVPTSDRGRFY 395
Query: 690 AFWTRFSLARLLPDKSSHHGTKLYTGKKEDLY 785
AF FS K G GKK+DL+
Sbjct: 396 AFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLF 427
Score = 112 bits (269), Expect = 6e-26
Identities = 50/94 (53%), Positives = 73/94 (77%), Gaps = 4/94 (4%)
Frame = +1
Query: 241 EQVMGENFFNPQTKKWSKQKDD----DNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLK 408
+++ GEN+FNP+TKKWSK D N+R+F M++LDPIY++FDA+M +K+E+ LL
Sbjct: 242 QRLWGENYFNPKTKKWSKSATDANGNSNQRAFNMFILDPIYRIFDAVMNSRKDEVFTLLS 301
Query: 409 KIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALL 510
K+ VTIK ++ + +GKALLKVVMR +LPA +AL+
Sbjct: 302 KLEVTIKPDEKELEGKALLKVVMRKFLPAADALM 335
Score = 94.3 bits (224), Expect = 2e-20
Identities = 43/83 (51%), Positives = 58/83 (69%)
Frame = +2
Query: 35 YQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADK 214
YQ F R+VE+VNV+I+TY D +G+ +V P KG+V F SGLHGWAFT++QF+ YA K
Sbjct: 175 YQNFARVVESVNVVISTYYDK--VLGDCQVFPDKGTVAFASGLHGWAFTVRQFANRYAKK 232
Query: 215 FKIDLVKLMNRLWEKTFSTLKRR 283
F ID K+M RLW + + K +
Sbjct: 233 FGIDRNKMMQRLWGENYFNPKTK 255
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 113 bits (271), Expect = 4e-26
Identities = 54/92 (58%), Positives = 64/92 (69%)
Frame = +3
Query: 510 QMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFY 689
+MI +HLPSP AQ+YR E LYEGP DDE A+GI++CD APLM+YVSKMVPTSD+GRFY
Sbjct: 336 EMIVLHLPSPKTAQQYRAETLYEGPMDDECAVGIRNCDANAPLMIYVSKMVPTSDRGRFY 395
Query: 690 AFWTRFSLARLLPDKSSHHGTKLYTGKKEDLY 785
AF FS K G GKK+DL+
Sbjct: 396 AFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLF 427
Score = 112 bits (269), Expect = 6e-26
Identities = 50/94 (53%), Positives = 73/94 (77%), Gaps = 4/94 (4%)
Frame = +1
Query: 241 EQVMGENFFNPQTKKWSKQKDD----DNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLK 408
+++ GEN+FNP+TKKWSK D N+R+F M++LDPIY++FDA+M +K+E+ LL
Sbjct: 242 QRLWGENYFNPKTKKWSKSATDANGNSNQRAFNMFILDPIYRIFDAVMNSRKDEVFTLLS 301
Query: 409 KIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALL 510
K+ VTIK ++ + +GKALLKVVMR +LPA +AL+
Sbjct: 302 KLEVTIKPDEKELEGKALLKVVMRKFLPAADALM 335
Score = 94.3 bits (224), Expect = 2e-20
Identities = 43/83 (51%), Positives = 58/83 (69%)
Frame = +2
Query: 35 YQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADK 214
YQ F R+VE+VNV+I+TY D +G+ +V P KG+V F SGLHGWAFT++QF+ YA K
Sbjct: 175 YQNFARVVESVNVVISTYYDK--VLGDCQVFPDKGTVAFASGLHGWAFTVRQFANRYAKK 232
Query: 215 FKIDLVKLMNRLWEKTFSTLKRR 283
F ID K+M RLW + + K +
Sbjct: 233 FGIDRNKMMQRLWGENYFNPKTK 255
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 47.6 bits (108), Expect = 2e-06
Identities = 28/99 (28%), Positives = 56/99 (56%), Gaps = 5/99 (5%)
Frame = +1
Query: 241 EQVMGENFFNPQTKKWSKQK-DDDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIG 417
+++ G+ +F+ +T+K++KQ D RSF ++L+P+YK+ + + E++ L
Sbjct: 352 KRLWGDIYFDSKTRKFAKQSLDGSGVRSFVHFILEPLYKLHTLTISDEAEKLKKHLSSFQ 411
Query: 418 VTIKHEDSDKDGKALLKVVMRSW--LPAG--EALLR*LP 522
+ +K +D D K LL+++ S+ P G A+ R +P
Sbjct: 412 IYLKPKDYLLDPKPLLQLICASFFGFPVGFVNAVTRHIP 450
Score = 39.9 bits (89), Expect = 4e-04
Identities = 26/84 (30%), Positives = 40/84 (47%), Gaps = 1/84 (1%)
Frame = +2
Query: 35 YQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADK 214
Y + +++ VN I + D + RV P G+V F S G+ FTL F+++Y D+
Sbjct: 286 YHKLRHVIDEVNDNICQISKDL----KYRVSPELGNVCFASCDLGYCFTLSSFAKLYIDR 341
Query: 215 F-KIDLVKLMNRLWEKTFSTLKRR 283
ID+ RLW + K R
Sbjct: 342 HGGIDVDLFSKRLWGDIYFDSKTR 365
Score = 37.1 bits (82), Expect = 0.003
Identities = 21/59 (35%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
Frame = +3
Query: 525 HLPSPVVAQKYRMEMLYEGPHDDEAAIGI--KSCDPEAPLMMYVSKMVPTSDKGRFYAF 695
H+PSP + Y GP + I S + APL+M+V+K+ T D FYAF
Sbjct: 448 HIPSPRENAARKASQSYIGPINSSIGKAILEMSREESAPLVMHVTKLYNTVDANNFYAF 506
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 45.6 bits (103), Expect = 8e-06
Identities = 35/108 (32%), Positives = 47/108 (43%), Gaps = 16/108 (14%)
Frame = +2
Query: 50 RIVENVNVIIATY----------NDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSE 199
R+VE VN +I T+ ND+ + P +G+V F S GWAF L QFSE
Sbjct: 176 RLVEQVNAVIGTFYTGELMQLADNDEVISDEGIYFAPEQGNVVFASAYDGWAFCLDQFSE 235
Query: 200 MYADKFKIDLVKLMNRLW------EKTFSTLKRRSGQNKRMMTTNVHF 325
Y K + L LW KT L+ + Q +R+ V F
Sbjct: 236 FYEKKLGLKQKALTKCLWGDYYLDPKTKRVLQPKHLQGRRLKPMFVQF 283
Score = 37.1 bits (82), Expect = 0.003
Identities = 21/57 (36%), Positives = 35/57 (61%), Gaps = 7/57 (12%)
Frame = +3
Query: 528 LPSPVVAQKYRM-EMLYEGPH----DDEAAIGIKSCDP--EAPLMMYVSKMVPTSDK 677
+PSP+ AQ R ++L PH D + + ++SCD E P+++Y+SKMV S++
Sbjct: 351 IPSPINAQANRARKVLSSTPHYEMIDPDITLAMESCDASKEQPVLVYISKMVAFSER 407
>SPAC24C9.11 |||MIF4G/MA4 domain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 775
Score = 27.9 bits (59), Expect = 1.8
Identities = 20/77 (25%), Positives = 36/77 (46%), Gaps = 4/77 (5%)
Frame = +1
Query: 250 MGENFFNPQTKKWSKQKDDDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIK 429
+ +++ + K +K+K NK++ +LDPI ++ EI L K +G+ K
Sbjct: 70 LNDDYLDAHRKSSTKKKSSQNKQAKESKLLDPIAFQHKIALEEDDREIAHLEKMLGIKSK 129
Query: 430 ----HEDSDKDGKALLK 468
H DK+ LL+
Sbjct: 130 DKSAHSKIDKEFGWLLE 146
>SPBC3B9.21 |dcp1||mRNA decapping complex subunit
Dcp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 127
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/31 (41%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = -1
Query: 420 DSNLLKEIINLLFLKFH-DGIEHLVDRIQNV 331
D N+L+ +NL LKFH IE ++D +V
Sbjct: 3 DENILRNAVNLQVLKFHYPEIESIIDIASHV 33
>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1364
Score = 27.5 bits (58), Expect = 2.3
Identities = 11/41 (26%), Positives = 22/41 (53%)
Frame = -1
Query: 741 ASFCPVTTLPEKNASKRRRNDLCRRSAPSCSRTSSVGLQDH 619
A + T+L + + RN+L + + + T+SVG+ +H
Sbjct: 784 ADYADSTSLERLESQEPNRNELVQVGSSNAGNTTSVGMNEH 824
>SPBC1198.03c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 249
Score = 26.6 bits (56), Expect = 4.0
Identities = 9/31 (29%), Positives = 16/31 (51%)
Frame = +2
Query: 173 AFTLKQFSEMYADKFKIDLVKLMNRLWEKTF 265
A+ + + E Y DK K ++K +W + F
Sbjct: 153 AYVISYYVEKYVDKAKFPIIKGFIEMWNRKF 183
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.2 bits (55), Expect = 5.4
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 576 EGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSD 674
+G H EA +K CDPE L + SK T++
Sbjct: 193 DGTHLAEA---LKKCDPETTLFLIASKTFTTAE 222
>SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 138
Score = 25.8 bits (54), Expect = 7.1
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 3/43 (6%)
Frame = -3
Query: 187 FEGESPPMKTRPETNRALARVDTH---LTHGTTIIVICGYNDV 68
+EGE P R ++ LA VD H L ++IV C Y+ V
Sbjct: 33 YEGERIPGSVRIPSDTFLASVDQHVDDLMKKRSLIVHCTYSQV 75
>SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 547
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/47 (27%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = +1
Query: 349 YKVFDAIMKFKKEEIDDLLKKIGVTIKHE---DSDKDGKALLKVVMR 480
Y+V + + F+K+ +D ++K + E +S K G+ ++ VV R
Sbjct: 350 YEVIEISIPFQKDMLDTVVKVFSADARFEINNESQKTGEPVVSVVKR 396
>SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1018
Score = 25.4 bits (53), Expect = 9.4
Identities = 9/28 (32%), Positives = 20/28 (71%)
Frame = -3
Query: 244 VHKLDKVNLEFVSIHLRELFEGESPPMK 161
+H ++K+N+E +++ + +F ESP +K
Sbjct: 716 LHFIEKLNIELLNLLINVVFVTESPGVK 743
>SPCC1393.06c |||rRNA processing protein Ipi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 408
Score = 25.4 bits (53), Expect = 9.4
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -1
Query: 411 LLKEIINLLFLKFHDGIEHLVDRIQNVHAK 322
LL+ I ++FL H G+ H+ I+N K
Sbjct: 133 LLEPNIGMIFLYTHSGMTHITPSIRNDSTK 162
>SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1272
Score = 25.4 bits (53), Expect = 9.4
Identities = 15/59 (25%), Positives = 28/59 (47%)
Frame = -2
Query: 452 PSLSESSCLIVTPIFLRRSSISSFLNFMMASNTL*IGSKTYMQNERLLSSSFCFDHFFV 276
P +S S C+ +F+ S S +F+M S L + S+ Y + + S+C + +
Sbjct: 417 PFISSSLCISPRYMFVVNSETVSVGSFLMVSEQLQMLSERYGFFDSIKFGSYCVSNLSI 475
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 25.4 bits (53), Expect = 9.4
Identities = 10/34 (29%), Positives = 16/34 (47%)
Frame = -3
Query: 211 VSIHLRELFEGESPPMKTRPETNRALARVDTHLT 110
+ + L+E+ + PP RP + D HLT
Sbjct: 269 IELSLKEMPQSREPPSYERPSEANVVISQDQHLT 302
>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 629
Score = 25.4 bits (53), Expect = 9.4
Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 3/62 (4%)
Frame = -3
Query: 709 EKRVQKA*KRPLSEVGTILLTYIISGASGSQLLIPMAASSSWGPSYNISIRYF---WATT 539
+ RV A +P+S+ T++ + +S + +P A + SY++S +Y WAT
Sbjct: 193 QHRVVGAYVKPVSKESTLVDGNPVCSSSSNPQYLPEGADLTLVTSYSVSWKYSDTPWATR 252
Query: 538 GD 533
D
Sbjct: 253 WD 254
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,561,479
Number of Sequences: 5004
Number of extensions: 78896
Number of successful extensions: 265
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 243
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 260
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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