Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0036.Seq
(697 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34910.1 68417.m04950 DEAD/DEAH box helicase, putative (RH16)... 69 4e-12
At5g08620.1 68418.m01025 DEAD box RNA helicase (RH25) identical ... 33 0.24
At2g07020.1 68415.m00803 protein kinase family protein contains ... 31 0.73
At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contai... 31 0.96
At2g46520.1 68415.m05798 cellular apoptosis susceptibility prote... 31 0.96
At3g19760.1 68416.m02501 eukaryotic translation initiation facto... 30 1.3
At4g37820.1 68417.m05351 expressed protein Kaposi's sarcoma-asso... 30 1.7
At5g08610.1 68418.m01024 DEAD box RNA helicase (RH26) strong sim... 29 2.2
At5g51280.1 68418.m06357 DEAD-box protein abstrakt, putative 29 2.9
At4g08310.1 68417.m01372 expressed protein glutamic acid-rich pr... 29 2.9
At1g30810.1 68414.m03767 transcription factor jumonji (jmj) fami... 29 2.9
At1g16280.1 68414.m01949 DEAD/DEAH box helicase, putative simila... 29 2.9
At4g14840.1 68417.m02281 expressed protein 29 3.9
At1g29350.1 68414.m03588 expressed protein 29 3.9
At1g35080.1 68414.m04347 hypothetical protein 28 5.1
At5g56800.1 68418.m07088 hypothetical protein 28 6.8
At2g30100.1 68415.m03663 ubiquitin family protein low similarity... 28 6.8
At1g17620.1 68414.m02179 expressed protein 28 6.8
At5g35670.1 68418.m04261 calmodulin-binding family protein conta... 27 9.0
At5g14440.1 68418.m01690 surfeit locus 2 protein-related / SURF2... 27 9.0
At2g17020.1 68415.m01962 F-box family protein (FBL10) contains s... 27 9.0
At1g29370.1 68414.m03591 kinase-related similar to putative prot... 27 9.0
At1g12290.1 68414.m01421 disease resistance protein (CC-NBS-LRR ... 27 9.0
>At4g34910.1 68417.m04950 DEAD/DEAH box helicase, putative (RH16)
identical to cDNA DEAD box RNA helicase, RH16 GI:3776006
Length = 626
Score = 68.5 bits (160), Expect = 4e-12
Identities = 30/65 (46%), Positives = 45/65 (69%)
Frame = -2
Query: 456 RAVTRIAVREARLKEIKQELLNCKKLQGYFEENPSDLAALRRDKALHTVKVQQHLAHVPE 277
++VT+IAVRE+R ++++ E++N +KL+ +FE NP DL LR DK L HL +PE
Sbjct: 489 KSVTKIAVRESRAQDLRNEIINSEKLKAHFEANPRDLDLLRHDKPLSKTAPAPHLKDIPE 548
Query: 276 YLLPA 262
YL+ A
Sbjct: 549 YLVDA 553
Score = 33.5 bits (73), Expect = 0.14
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = -1
Query: 697 FGYEDEIKELLSHLPKIYQAVLASATLSDDV 605
+GYED ++ + S +P+ Q +L SAT S DV
Sbjct: 215 YGYEDNLRSVTSIIPRRCQCLLMSATTSSDV 245
>At5g08620.1 68418.m01025 DEAD box RNA helicase (RH25) identical to
RNA helicase [Arabidopsis thaliana] GI:3776023; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 563
Score = 32.7 bits (71), Expect = 0.24
Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 3/65 (4%)
Frame = -1
Query: 694 GYEDEIKELLSHLPKIYQAVLASATLSDDVLSFVSI---REKPLMDAVEQHLSNGFKGQK 524
G+ EI+ +++ +PK Q L SAT+SD+V + R+ ++ V++ G QK
Sbjct: 251 GFRREIERIIAAVPKQRQTFLFSATVSDEVRQICHVALKRDHEFVNCVQE--GAGETHQK 308
Query: 523 VLQKY 509
V Q Y
Sbjct: 309 VSQMY 313
>At2g07020.1 68415.m00803 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 700
Score = 31.1 bits (67), Expect = 0.73
Identities = 20/49 (40%), Positives = 27/49 (55%)
Frame = -1
Query: 688 EDEIKELLSHLPKIYQAVLASATLSDDVLSFVSIREKPLMDAVEQHLSN 542
+DEI+ +L K Y A +SD LSFVS ++P MD E + SN
Sbjct: 198 QDEIEIKSPYLRKEYDQGTYQALVSDSDLSFVS-SDRPSMDWFEDNRSN 245
>At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contains
Pfam profiles PF00270:DEAD/DEAH box helicase and
PF00271: Helicase conserved C-terminal domain;
identical to cDNA RH27 helicase, partial GI:4033334
Length = 633
Score = 30.7 bits (66), Expect = 0.96
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = -1
Query: 247 DIVEDQETVTEKPQVKKRKQNANFGSAKRHKYQA-RQNDPLKSFDVKKKKQTAGET 83
D E+ + E+P+ KK+K+N G + +A + +P K KK Q G+T
Sbjct: 47 DAKENNALIDEEPKKKKKKKNKKRGDTDDGEDEAVAEEEPKKKKKKNKKLQQRGDT 102
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/47 (38%), Positives = 28/47 (59%), Gaps = 2/47 (4%)
Frame = -1
Query: 691 YEDEIKELLSHLPKIYQAVLASATLSDDV--LSFVSIREKPLMDAVE 557
+E+++K++L+ LPK Q L SAT S V L+ VS+ +D E
Sbjct: 318 FEEDLKKILNLLPKTRQTSLFSATQSAKVEDLARVSLTSPVYIDVDE 364
>At2g46520.1 68415.m05798 cellular apoptosis susceptibility protein,
putative / importin-alpha re-exporter, putative similar
to cellular apoptosis susceptibility protein [Homo
sapiens] GI:3598795, SP|Q9ERK4 Importin-alpha re-exporter
(Chromosome segregation 1-like protein) (Cellular
apoptosis susceptibility protein) {Mus musculus};
contains Pfam profiles PF03810: Importin-beta N-terminal
domain, PF03378: CAS/CSE protein C-terminus
Length = 972
Score = 30.7 bits (66), Expect = 0.96
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
Frame = -1
Query: 235 DQETVTEKPQVKKRKQNANFGSA--KRHKYQARQNDPLKSFDVKKKKQ 98
+QE V ++P++ + +N + +A K H ++ DPLK D+K KQ
Sbjct: 882 EQERVLDEPEMPEISENVGYTAAFVKLHNAGKKEEDPLK--DIKDPKQ 927
>At3g19760.1 68416.m02501 eukaryotic translation initiation factor
4A, putative / eIF-4A, putative / DEAD box RNA helicase,
putative contains DEAD/DEAH helicase domain; similar to
RNA helicase GB:CAA09195 from [Arabidopsis thaliana];
identical to cDNA DEAD box RNA helicase, RH2 GI:3775984
Length = 408
Score = 30.3 bits (65), Expect = 1.3
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = -1
Query: 694 GYEDEIKELLSHLPKIYQAVLASATLSDDVLSFVS 590
G++D+I ++ +LP Q L SATL ++L S
Sbjct: 193 GFKDQIYDVYRYLPPDLQVCLVSATLPHEILEMTS 227
>At4g37820.1 68417.m05351 expressed protein Kaposi's
sarcoma-associated herpes-like virus ORF73gene, Kaposi's
sarcoma-associated herpesvirus, U52064
Length = 532
Score = 29.9 bits (64), Expect = 1.7
Identities = 13/62 (20%), Positives = 33/62 (53%)
Frame = -1
Query: 283 TRIPASGCPANEDIVEDQETVTEKPQVKKRKQNANFGSAKRHKYQARQNDPLKSFDVKKK 104
+++ SG + E+ EKP+ KK++++++ G K + + R+ + S + K+
Sbjct: 310 SKVKESGKNEKDASSSQDESKEEKPERKKKEESSSQGEGKEEEPEKREKEDSSSQEESKE 369
Query: 103 KQ 98
++
Sbjct: 370 EE 371
>At5g08610.1 68418.m01024 DEAD box RNA helicase (RH26) strong
similarity to RNA helicase RH26 [Arabidopsis thaliana]
GI:3776025; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain; identical to cDNA DEAD box RNA helicase, RH26
GI:3776024
Length = 850
Score = 29.5 bits (63), Expect = 2.2
Identities = 18/65 (27%), Positives = 35/65 (53%), Gaps = 3/65 (4%)
Frame = -1
Query: 694 GYEDEIKELLSHLPKIYQAVLASATLSDDVLSFVSI---REKPLMDAVEQHLSNGFKGQK 524
G+ +I+ +++ +PK Q L SAT+ ++V + R+ ++ V++ +G QK
Sbjct: 553 GFRRDIERIIAAVPKQRQTFLFSATVPEEVRQICHVALKRDHEFINCVQE--GSGETHQK 610
Query: 523 VLQKY 509
V Q Y
Sbjct: 611 VTQMY 615
>At5g51280.1 68418.m06357 DEAD-box protein abstrakt, putative
Length = 591
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = -1
Query: 694 GYEDEIKELLSHLPKIYQAVLASATLSDDVLSFV-SIREKPL 572
G+ED+I+E+ H Q +L SAT+ + F S KP+
Sbjct: 318 GFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPV 359
>At4g08310.1 68417.m01372 expressed protein glutamic acid-rich
protein precursor - Plasmodium falciparum, PIR2:A54514
Length = 504
Score = 29.1 bits (62), Expect = 2.9
Identities = 18/56 (32%), Positives = 28/56 (50%)
Frame = -1
Query: 250 EDIVEDQETVTEKPQVKKRKQNANFGSAKRHKYQARQNDPLKSFDVKKKKQTAGET 83
ED ED+E +K +KRK + + G+ K R+ + K KK KQT ++
Sbjct: 269 EDEEEDKEVAVKKKMAEKRKLSKSEGTGK------RKREKEKPASAKKTKQTDSQS 318
>At1g30810.1 68414.m03767 transcription factor jumonji (jmj) family
protein / zinc finger (C5HC2 type) family protein
contains similarity to Swiss-Prot:P29375
retinoblastoma-binding protein 2 (RBBP-2) [Homo
sapiens]; contains Pfam domains PF02375: jmjN domain and
PF02373: jmjC domain; intron between exons 6 and 7 was
required to circumvent a frameshift. There could be an
underlying sequence error.
Length = 787
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/31 (38%), Positives = 24/31 (77%), Gaps = 2/31 (6%)
Frame = -1
Query: 247 DIVEDQETVTEKPQVKKRK--QNANFGSAKR 161
D+++++E + +KP+ +KRK +N+ GS+KR
Sbjct: 122 DLLQNREPMKKKPKSRKRKRRRNSRMGSSKR 152
>At1g16280.1 68414.m01949 DEAD/DEAH box helicase, putative similar
to gb|L13612 DEAD-box protein (dbp45A) from Drosophila
melanogaster and is a member of PF|00270 DEAD/DEAH box
helicase family
Length = 491
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/30 (36%), Positives = 21/30 (70%)
Frame = -1
Query: 694 GYEDEIKELLSHLPKIYQAVLASATLSDDV 605
G++DE++ + LPK Q +L SAT++ ++
Sbjct: 219 GFQDELRTIFQCLPKSRQTLLFSATMTSNL 248
>At4g14840.1 68417.m02281 expressed protein
Length = 555
Score = 28.7 bits (61), Expect = 3.9
Identities = 19/73 (26%), Positives = 30/73 (41%)
Frame = -1
Query: 301 ATFGTCTRIPASGCPANEDIVEDQETVTEKPQVKKRKQNANFGSAKRHKYQARQNDPLKS 122
A F R+ A+ E E Q + VKK + + KR + Q + N+P +
Sbjct: 160 ALFQPAERVDAASL-FEETSKEGQNQDEDAEDVKKEGSTSRYSQRKRKQVQIQTNEPKAT 218
Query: 121 FDVKKKKQTAGET 83
+K+TA T
Sbjct: 219 PRASNRKKTATTT 231
>At1g29350.1 68414.m03588 expressed protein
Length = 831
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = -1
Query: 214 KPQVKKRKQNANFGSAKRHKYQ--ARQNDPLK 125
+PQ K KQN N GS H+++ A Q P+K
Sbjct: 201 RPQNKTTKQNVNVGSEINHEHEVNANQQAPVK 232
>At1g35080.1 68414.m04347 hypothetical protein
Length = 386
Score = 28.3 bits (60), Expect = 5.1
Identities = 20/58 (34%), Positives = 33/58 (56%), Gaps = 6/58 (10%)
Frame = -2
Query: 396 LNCKKLQGYFEENPSDLAALRRDK-ALHTVK---VQQHLAHVP--EYLLPAALPMKIL 241
L C K+ G+F PS LA++R ++ A+H + VQ HL P ++ +P + KI+
Sbjct: 133 LQCDKVLGHFWRPPSALASIRGNQFAVHPPRQALVQFHLQPPPFSDFHMPEHVAAKIV 190
>At5g56800.1 68418.m07088 hypothetical protein
Length = 344
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/39 (41%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
Frame = +1
Query: 406 FDLFESSFSDCYPGDGAPRSL-ERYLNPSTSSSANYTFV 519
FDL E S CY PRSL R+ N T +FV
Sbjct: 30 FDLSEVSIESCYGSQTLPRSLRSRHWNLKTLKLKKLSFV 68
>At2g30100.1 68415.m03663 ubiquitin family protein low similarity to
SP|Q9UQ13 Leucine-rich repeat protein SHOC-2
(Ras-binding protein Sur-8) {Homo sapiens}; contains
Pfam profiles PF00240: Ubiquitin family, PF01535: PPR
repeat, PF00560: Leucine Rich Repeat
Length = 897
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -2
Query: 459 WRAVTRIAVREARLKEIKQELLNC 388
W+A IA+ +A LKEI +E+ +C
Sbjct: 651 WKATGVIALAQANLKEIPEEVWDC 674
>At1g17620.1 68414.m02179 expressed protein
Length = 264
Score = 27.9 bits (59), Expect = 6.8
Identities = 15/34 (44%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
Frame = +2
Query: 254 GRAAGSRYSGTCAKCCC-TFTVCSALSLLNAARS 352
GR S G C +CCC T V L L+ AA S
Sbjct: 47 GRRRTSHTRGCCCRCCCWTIFVIILLLLIVAAAS 80
>At5g35670.1 68418.m04261 calmodulin-binding family protein contains
IQ calmodulin-binding motif, Pfam:PF00612
Length = 442
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = -1
Query: 214 KPQVKKRKQNANFGS-AKRHKYQARQNDPLKSFDVKKKKQT 95
KP + KRK ++ S K HK QA ++D + KK +T
Sbjct: 380 KPSISKRKSVPSYKSQRKHHKLQATKSDLQQQTKKAKKAKT 420
>At5g14440.1 68418.m01690 surfeit locus 2 protein-related / SURF2
protein-related contains weak hit to Pfam profile
PF05477: Surfeit locus protein 2 (SURF2)
Length = 291
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = -1
Query: 277 IPASG--CPANEDIVEDQETVTEKPQVKKRKQNANFGSAKRH 158
IPA G PA E+ VED++ +K + K+K+N K++
Sbjct: 124 IPAEGGETPAKENGVEDEDKKKKKKKNNKKKKNKKSVEKKKN 165
>At2g17020.1 68415.m01962 F-box family protein (FBL10) contains
similarity to F-box protein Partner of Paired
GI:10441427 from [Drosophila melanogaster]
Length = 656
Score = 27.5 bits (58), Expect = 9.0
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -1
Query: 685 DEIKELLSHLPKIYQAVLASATLSDDVLSFV 593
DE +SHLPK+ +L A +SD LS++
Sbjct: 418 DETLTAVSHLPKLKVLLLDGADISDTGLSYL 448
>At1g29370.1 68414.m03591 kinase-related similar to putative protein
kinase (GI:11125348) [Homo sapiens]; similar to Paired
box protein Pax-8 (Swiss-Prot:P47240) [Canis familiaris]
Length = 831
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -1
Query: 214 KPQVKKRKQNANFGSAKRHKYQARQN 137
+PQ K KQN N GS H+++ N
Sbjct: 201 RPQNKTTKQNVNMGSEINHEHKVSAN 226
>At1g12290.1 68414.m01421 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 884
Score = 27.5 bits (58), Expect = 9.0
Identities = 18/63 (28%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = -2
Query: 552 ISLMDSKDKKFYKSIICTRRSGRV-QIPLQRPWRAVTRIAVREARLKEIKQELLNCKKLQ 376
++L + D + +K R R+ +IP + W+ V+R+++ R+KEI C KL
Sbjct: 485 MALWIASDLRKHKDNCIVRAGFRLNEIPKVKDWKVVSRMSLVNNRIKEIHGS-PECPKLT 543
Query: 375 GYF 367
F
Sbjct: 544 TLF 546
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,037,210
Number of Sequences: 28952
Number of extensions: 292359
Number of successful extensions: 1028
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 1001
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1028
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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