BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0034.Seq (678 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 141 7e-35 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 140 2e-34 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 117 1e-27 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 106 3e-24 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 83 3e-17 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 69 5e-13 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 62 5e-11 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 58 2e-09 SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomy... 27 2.5 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 4.4 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 26 5.8 SPBC14F5.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 7.6 SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 7.6 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 141 bits (342), Expect = 7e-35 Identities = 69/84 (82%), Positives = 77/84 (91%) Frame = +2 Query: 2 RAKRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 181 RAKRTLSSS QASI IDSLFEGIDFYTSITRARFEEL ADLFR TMEPVE+ LRD+K+DK Sbjct: 267 RAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTMEPVERVLRDSKVDK 326 Query: 182 AQIHDIVLVGGSTRIPKVQKLLQD 253 A +++IVLVGGSTRIP+VQKL+ D Sbjct: 327 ASVNEIVLVGGSTRIPRVQKLVSD 350 Score = 107 bits (256), Expect = 2e-24 Identities = 50/55 (90%), Positives = 53/55 (96%) Frame = +3 Query: 510 LIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEK 674 LIQVFEGERA TKD NLLGKFEL+GIPPAPRGVPQIEVTFD+DANGILNVSA+EK Sbjct: 437 LIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSALEK 491 Score = 77.4 bits (182), Expect = 2e-15 Identities = 43/94 (45%), Positives = 48/94 (51%) Frame = +1 Query: 226 PQGAEAPARFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGI 405 P+ + + FFNGKE KSINPDE +L GD SE+ QD GI Sbjct: 342 PRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGI 401 Query: 406 ETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPG 507 ETAGGVMT LIKRN YSDNQPG Sbjct: 402 ETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPG 435 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 140 bits (339), Expect = 2e-34 Identities = 67/84 (79%), Positives = 78/84 (92%) Frame = +2 Query: 2 RAKRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 181 RAKRTLSSS QASI IDSL+EGIDFYTSITRARFEEL ADLFR+TMEPVEK LRD+K+DK Sbjct: 267 RAKRTLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTMEPVEKVLRDSKIDK 326 Query: 182 AQIHDIVLVGGSTRIPKVQKLLQD 253 + +++IVLVGGSTRIP++QKL+ D Sbjct: 327 SSVNEIVLVGGSTRIPRIQKLVSD 350 Score = 107 bits (256), Expect = 2e-24 Identities = 50/55 (90%), Positives = 53/55 (96%) Frame = +3 Query: 510 LIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEK 674 LIQVFEGERA TKD NLLGKFEL+GIPPAPRGVPQIEVTFD+DANGILNVSA+EK Sbjct: 437 LIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSALEK 491 Score = 75.8 bits (178), Expect = 5e-15 Identities = 42/85 (49%), Positives = 44/85 (51%) Frame = +1 Query: 253 FFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTT 432 FFNGKE KSINPDE IL GD SE+ QD GIETAGGVMT Sbjct: 351 FFNGKEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTP 410 Query: 433 LIKRNXXXXXXXXXXXXXYSDNQPG 507 LIKRN Y+DNQPG Sbjct: 411 LIKRNTTIPTKKSEVFSTYADNQPG 435 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 117 bits (282), Expect = 1e-27 Identities = 54/84 (64%), Positives = 70/84 (83%) Frame = +2 Query: 2 RAKRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 181 RAKR LSS TQ ++ +DSL GIDF +SITRARFE++NA F++T++PV K L+D+K+ K Sbjct: 271 RAKRALSSVTQTTVEVDSLSNGIDFSSSITRARFEDINATTFKATIDPVAKVLKDSKVPK 330 Query: 182 AQIHDIVLVGGSTRIPKVQKLLQD 253 A +HDIVLVGGSTRIPKVQ+L+ D Sbjct: 331 ADVHDIVLVGGSTRIPKVQRLVSD 354 Score = 72.1 bits (169), Expect = 7e-14 Identities = 30/53 (56%), Positives = 42/53 (79%) Frame = +3 Query: 519 VFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKS 677 V++GER +N LG+F+LTGIPP PRG ++E TF++DANGIL V+A+EK+ Sbjct: 445 VYQGERVNCAENEPLGEFQLTGIPPMPRGQAELEATFELDANGILKVTAVEKT 497 Score = 37.5 bits (83), Expect = 0.002 Identities = 24/84 (28%), Positives = 33/84 (39%), Gaps = 1/84 (1%) Frame = +1 Query: 253 FFNGKELNKSINPDEXXXXXXXXXXXIL-HGDKSEEVQDXXXXXXXXXXXGIETAGGVMT 429 FF+G+ LNKSINPDE +L + S++ QD G+ G V Sbjct: 355 FFDGRALNKSINPDEAVAYGAAVQAAVLTNKADSDKTQDLLLLDVVPLSLGVAMEGNVFG 414 Query: 430 TLIKRNXXXXXXXXXXXXXYSDNQ 501 + RN +DNQ Sbjct: 415 VVCPRNTPIPTIKKRTFTTVADNQ 438 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 106 bits (254), Expect = 3e-24 Identities = 49/94 (52%), Positives = 71/94 (75%) Frame = +2 Query: 2 RAKRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 181 +AKRTLSS I I+S F G DF +++RA+FEE+N DLF+ T++PVE+ L+D+ + K Sbjct: 299 KAKRTLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLKK 358 Query: 182 AQIHDIVLVGGSTRIPKVQKLLQDSLMERSSTNL 283 ++I DIVLVGGSTRIPKVQ+LL+ +++S + Sbjct: 359 SEIDDIVLVGGSTRIPKVQELLESFFGKKASKGI 392 Score = 103 bits (247), Expect = 2e-23 Identities = 45/56 (80%), Positives = 53/56 (94%) Frame = +3 Query: 510 LIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKS 677 LIQV+EGER +TKDNNLLGKF+L GIPPAPRGVPQIEVTF++DANG+L VSA++KS Sbjct: 466 LIQVYEGERTLTKDNNLLGKFDLRGIPPAPRGVPQIEVTFEVDANGVLTVSAVDKS 521 Score = 41.9 bits (94), Expect = 8e-05 Identities = 27/74 (36%), Positives = 35/74 (47%) Frame = +1 Query: 226 PQGAEAPARFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGI 405 P+ E FF GK+ +K INPDE +L G++ + + GI Sbjct: 374 PKVQELLESFF-GKKASKGINPDEAVAYGAAVQAGVLSGEEGSD--NIVLLDVIPLTLGI 430 Query: 406 ETAGGVMTTLIKRN 447 ET GGVMT LI RN Sbjct: 431 ETTGGVMTKLIGRN 444 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 83.4 bits (197), Expect = 3e-17 Identities = 35/55 (63%), Positives = 48/55 (87%) Frame = +3 Query: 513 IQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKS 677 I+VF+GER + +DN L+G F+LTGI PAP+G PQIEV+FD+DA+GI+NVSA +K+ Sbjct: 481 IRVFQGERELVRDNKLIGNFQLTGIAPAPKGQPQIEVSFDVDADGIINVSARDKA 535 Score = 55.2 bits (127), Expect = 8e-09 Identities = 24/61 (39%), Positives = 47/61 (77%) Frame = +2 Query: 86 ITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDSLME 265 I+RA+FE+L L R T++P +++L+DA + ++I++++LVGG TR+P+V + ++ S+ + Sbjct: 343 ISRAQFEKLVDPLVRRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVK-SIFK 401 Query: 266 R 268 R Sbjct: 402 R 402 Score = 37.5 bits (83), Expect = 0.002 Identities = 27/80 (33%), Positives = 33/80 (41%) Frame = +1 Query: 208 GWLHSYPQGAEAPARFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXX 387 G + P+ E F +E KS+NPDE +L G V+D Sbjct: 384 GGMTRMPRVVETVKSIFK-REPAKSVNPDEAVAIGAAIQGGVLSG----HVKDLVLLDVT 438 Query: 388 XXXXGIETAGGVMTTLIKRN 447 GIET GGV T LI RN Sbjct: 439 PLSLGIETLGGVFTRLINRN 458 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 69.3 bits (162), Expect = 5e-13 Identities = 33/84 (39%), Positives = 54/84 (64%) Frame = +2 Query: 2 RAKRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 181 R K LS++++A I+ L +GIDF ITR+ E L D+ + +EP+ K+L+ A + Sbjct: 303 RVKHILSANSEAIASIEELADGIDFRLKITRSVLESLCKDMEDAAVEPINKALKKANLTF 362 Query: 182 AQIHDIVLVGGSTRIPKVQKLLQD 253 ++I+ I+L GG++RIP +Q L D Sbjct: 363 SEINSIILFGGASRIPFIQSTLAD 386 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 62.5 bits (145), Expect = 5e-11 Identities = 29/88 (32%), Positives = 52/88 (59%) Frame = +2 Query: 8 KRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQ 187 KR LS+ T AS +DSL +GIDF++SI R R++ + + V +++ A M+ Sbjct: 295 KRVLSNGTTASAAVDSLADGIDFHSSINRLRYDLAASATLNRMADLVTEAVEKANMEPFD 354 Query: 188 IHDIVLVGGSTRIPKVQKLLQDSLMERS 271 I +++L GG++ PK+ L++ E++ Sbjct: 355 ISEVILAGGASNTPKLTSLMESIFPEQT 382 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 57.6 bits (133), Expect = 2e-09 Identities = 28/82 (34%), Positives = 48/82 (58%) Frame = +2 Query: 2 RAKRTLSSSTQASIXIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 181 R K+ LS++ A + ++ + ID + I R+ FEEL L P+EK+L A + K Sbjct: 275 RLKKVLSANANAPLNVEMIMNDIDASSFIKRSDFEELIKPLLERLTPPIEKALELAGIKK 334 Query: 182 AQIHDIVLVGGSTRIPKVQKLL 247 ++ I +VGG TR+P V++++ Sbjct: 335 EDLYSIEMVGGCTRVPIVKEVI 356 >SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 27.1 bits (57), Expect = 2.5 Identities = 18/46 (39%), Positives = 23/46 (50%) Frame = -3 Query: 661 ETLRIPLASMSKVTSI*GTPRGAGGIPVSSNLPSKLLSLVIARSPS 524 E L PLA MS TSI G G GG P + + + + +SPS Sbjct: 76 EVLVDPLADMSAGTSIMGNNFGFGGFPRMNRRQRRRMG-IFDQSPS 120 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 4.4 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -2 Query: 194 RGFEPYPSWHHGETSPLAP 138 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 25.8 bits (54), Expect = 5.8 Identities = 17/53 (32%), Positives = 27/53 (50%) Frame = +2 Query: 53 SLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVG 211 SL G+D+YT I E +A +S+ E +K D + D++ D + VG Sbjct: 331 SLARGLDYYTGIIYEAVTEASAPKIKSSAE--KKKSADPEADRSN-DDSIGVG 380 >SPBC14F5.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 515 Score = 25.4 bits (53), Expect = 7.6 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = -1 Query: 306 SYGLVRVNRFV-ELLSIKESCRSFCTLGIRVEPPTSTIS 193 SY L N+++ ++S SC + LG + E +TIS Sbjct: 242 SYALNESNQYITSIVSPDSSCENIRILGFKAETNAATIS 280 >SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 7.6 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 4/34 (11%) Frame = +3 Query: 120 ICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 209 ICS LP + W + S PR+ R S ++ WW Sbjct: 133 ICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,735,403 Number of Sequences: 5004 Number of extensions: 54799 Number of successful extensions: 189 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 188 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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