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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0014.Seq
         (636 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g04460.1 68418.m00443 expressed protein                             33   0.16 
At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical t...    28   4.5  
At5g42320.1 68418.m05150 zinc carboxypeptidase family protein co...    28   6.0  
At5g13280.1 68418.m01525 aspartate kinase identical to aspartate...    28   6.0  

>At5g04460.1 68418.m00443 expressed protein
          Length = 863

 Score = 33.1 bits (72), Expect = 0.16
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +3

Query: 183 RLNGGNFSKNVPTKESPPPSPRWRHD 260
           +LNG N S+ +PT  +PPP P W  D
Sbjct: 694 QLNGINGSQLLPTPPAPPPQPIWHQD 719


>At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical to
           gi:3928758 contains Pfam profile PF04055: radical SAM
           domain protein
          Length = 374

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +3

Query: 186 LNGGNFSKNVPTKESPPPSPRW-RHDASSEERLGHIQLSKVPSSLRI 323
           ++G  +S  V TK+ P P P+W +      ER  ++Q+ K    L++
Sbjct: 55  IHGDTYSVEVGTKKKPLPKPKWMKESIPGGER--YVQIKKKLRDLKL 99


>At5g42320.1 68418.m05150 zinc carboxypeptidase family protein
           contains Pfam domain, PF00246: Zinc carboxypeptidase
          Length = 461

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 20/57 (35%), Positives = 26/57 (45%)
 Frame = -3

Query: 283 PNRSSLLAS*RHLGEGGGDSFVGTFLEKLPPLRRNKICSRKLTLRSIMILIFFFTEC 113
           P  SSL  S R  G     S     L  LPPL    +  RK+   S++I+I F+  C
Sbjct: 25  PFLSSLPLSRR--GHASAASLPANLLRGLPPLTIVVVTRRKIHF-SVLIIIVFYVRC 78


>At5g13280.1 68418.m01525 aspartate kinase identical to aspartate
           kinase [Arabidopsis thaliana] GI:4376158
          Length = 569

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 18/54 (33%), Positives = 29/54 (53%)
 Frame = -3

Query: 226 SFVGTFLEKLPPLRRNKICSRKLTLRSIMILIFFFTECLYFRKISGFCRNL*VK 65
           S + T+LE+L  L +     ++LTLR+   L+  F ECL  R  + +   + VK
Sbjct: 172 SVILTYLEELEQLLKGIAMMKELTLRTRDYLV-SFGECLSTRIFAAYLNTIGVK 224


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,052,517
Number of Sequences: 28952
Number of extensions: 227425
Number of successful extensions: 826
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 826
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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