BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0014.Seq (636 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g04460.1 68418.m00443 expressed protein 33 0.16 At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical t... 28 4.5 At5g42320.1 68418.m05150 zinc carboxypeptidase family protein co... 28 6.0 At5g13280.1 68418.m01525 aspartate kinase identical to aspartate... 28 6.0 >At5g04460.1 68418.m00443 expressed protein Length = 863 Score = 33.1 bits (72), Expect = 0.16 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = +3 Query: 183 RLNGGNFSKNVPTKESPPPSPRWRHD 260 +LNG N S+ +PT +PPP P W D Sbjct: 694 QLNGINGSQLLPTPPAPPPQPIWHQD 719 >At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical to gi:3928758 contains Pfam profile PF04055: radical SAM domain protein Length = 374 Score = 28.3 bits (60), Expect = 4.5 Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = +3 Query: 186 LNGGNFSKNVPTKESPPPSPRW-RHDASSEERLGHIQLSKVPSSLRI 323 ++G +S V TK+ P P P+W + ER ++Q+ K L++ Sbjct: 55 IHGDTYSVEVGTKKKPLPKPKWMKESIPGGER--YVQIKKKLRDLKL 99 >At5g42320.1 68418.m05150 zinc carboxypeptidase family protein contains Pfam domain, PF00246: Zinc carboxypeptidase Length = 461 Score = 27.9 bits (59), Expect = 6.0 Identities = 20/57 (35%), Positives = 26/57 (45%) Frame = -3 Query: 283 PNRSSLLAS*RHLGEGGGDSFVGTFLEKLPPLRRNKICSRKLTLRSIMILIFFFTEC 113 P SSL S R G S L LPPL + RK+ S++I+I F+ C Sbjct: 25 PFLSSLPLSRR--GHASAASLPANLLRGLPPLTIVVVTRRKIHF-SVLIIIVFYVRC 78 >At5g13280.1 68418.m01525 aspartate kinase identical to aspartate kinase [Arabidopsis thaliana] GI:4376158 Length = 569 Score = 27.9 bits (59), Expect = 6.0 Identities = 18/54 (33%), Positives = 29/54 (53%) Frame = -3 Query: 226 SFVGTFLEKLPPLRRNKICSRKLTLRSIMILIFFFTECLYFRKISGFCRNL*VK 65 S + T+LE+L L + ++LTLR+ L+ F ECL R + + + VK Sbjct: 172 SVILTYLEELEQLLKGIAMMKELTLRTRDYLV-SFGECLSTRIFAAYLNTIGVK 224 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,052,517 Number of Sequences: 28952 Number of extensions: 227425 Number of successful extensions: 826 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 782 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 826 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1305036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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