BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0006.Seq (765 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g23260.1 68417.m03353 protein kinase family protein contains ... 30 1.9 At1g64405.1 68414.m07300 expressed protein 28 5.9 At1g66840.1 68414.m07597 expressed protein contains Pfam profile... 28 7.8 >At4g23260.1 68417.m03353 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain Length = 579 Score = 29.9 bits (64), Expect = 1.9 Identities = 24/89 (26%), Positives = 47/89 (52%) Frame = -3 Query: 307 RKDQGRRKLPEWIVSFNLNKKM*S**LHNKQRNSVPEKGFEKFKEDINFNGSE*SLRRIM 128 RK + LP V F+L K + S + +RN + + GF + + + NG+E +++R+ Sbjct: 232 RKQKQEMDLPTESVQFDL-KTIESATSNFSERNKLGKGGFGEVYKGMLMNGTEIAVKRLS 290 Query: 127 KELGFRWKKRTENNRKLVNEKSNIRLLRI 41 K G + + +N +V + +I L+R+ Sbjct: 291 KTSG-QGEVEFKNEVVVVAKLQHINLVRL 318 >At1g64405.1 68414.m07300 expressed protein Length = 118 Score = 28.3 bits (60), Expect = 5.9 Identities = 15/58 (25%), Positives = 33/58 (56%) Frame = +3 Query: 201 GTEFRCLLCSYYDYIFLLRLNETIHSGNFLLP*SFLTRSPKHGEQARAFSFVNNAPNS 374 G + + +L + +FLL++N + H GN ++ + K +A +FS ++++P+S Sbjct: 41 GMKVKIVLTRHELDMFLLQMNRS-HDGNLMITKDVMVELEKRIIRASSFSSLSSSPSS 97 >At1g66840.1 68414.m07597 expressed protein contains Pfam profile PF05701: Plant protein of unknown function (DUF827); expression supported by MPSS Length = 607 Score = 27.9 bits (59), Expect = 7.8 Identities = 27/119 (22%), Positives = 56/119 (47%), Gaps = 2/119 (1%) Frame = -2 Query: 440 KQGKNDIEKLKQTSEATKTSVCTVRCIINEAKGSGLLTVFRTPGKKRSGKKKVTG-MDSF 264 ++GK+++ LK+ +EAT+ + I A+ L+ T K+ KK T +D Sbjct: 265 ERGKDNLSVLKEVTEATEAKKAELASI--NAELFCLVNTMDTLRKEFDHAKKETAWLDKM 322 Query: 263 VQS**KDVIIITT*QAAKLRTGERLRKI*R-RYKF*WLGMKLTTNYERARLQMEKKNRK 90 +Q KD +++ L ++L + + + +L LTT++E+ + E ++ Sbjct: 323 IQ---KDDVMLERLNTKLLIAKDQLEAVSKAEERISYLADNLTTSFEKLKSDREAAKKE 378 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,942,708 Number of Sequences: 28952 Number of extensions: 302332 Number of successful extensions: 761 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 745 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 761 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1712086600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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