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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS331G01f
         (521 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    31   0.10 
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto...    26   3.0  
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S...    25   6.8  
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo...    25   9.0  

>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
 Frame = +3

Query: 249 VSAYNSTNLFSFDLISPRLNL--DKGIQDVSVKKVPMPKRQT 368
           +S   +TN  + + +SP LNL  +  +QDVS+ K  M K+Q+
Sbjct: 145 LSINRNTNKKALNAVSPSLNLSSNSSVQDVSIDKEEMMKKQS 186


>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
           Sec74|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 928

 Score = 26.2 bits (55), Expect = 3.0
 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +3

Query: 252 SAYNSTNLFSFDLISPRLNLDKGIQDVS-VKKVPMPKRQTTSRSASYNCSQTIL 410
           SAY   +    D  S   +  K    VS   K+ +PKR  +S+S+S  CS   L
Sbjct: 74  SAYTGDSTTDLDSKSGHSSSQKLSNKVSSALKLTIPKRWRSSKSSSSQCSSPFL 127


>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
           Vps33|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 10/26 (38%), Positives = 19/26 (73%), Gaps = 1/26 (3%)
 Frame = -1

Query: 512 SFFEEVSELNHIFVSHR*S-TKFNFI 438
           ++F+++ +L H+ VSH  S T+FN +
Sbjct: 351 NYFQKLLQLQHLLVSHADSFTQFNLL 376


>SPAC22F3.12c |rgs1||regulator of G-protein signaling
           Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 481

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 9/16 (56%), Positives = 12/16 (75%)
 Frame = +1

Query: 256 PTIVPIYLVSTSSPHD 303
           P +VP+Y VS+ SP D
Sbjct: 228 PLLVPVYSVSSPSPKD 243


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,979,607
Number of Sequences: 5004
Number of extensions: 37124
Number of successful extensions: 69
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 67
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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