BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS329G02f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 71 1e-13
SPBC947.12 |kms2||spindle pole body protein Kms2|Schizosaccharom... 30 0.18
SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||M... 28 0.97
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 26 3.0
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 25 6.8
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 25 9.0
>SPAC57A7.04c |pabp||mRNA export shuttling protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 653
Score = 70.9 bits (166), Expect = 1e-13
Identities = 36/72 (50%), Positives = 49/72 (68%)
Frame = +1
Query: 43 EPLTSTMLAAAPLQEQKQMLGERLFPLIQRMHPDLAGKITGMLLEIDNSELLHMLEHAES 222
E T+ LAA P + +KQ+LGE L+P + L+GKITGMLLE+ NSELL +LE +
Sbjct: 570 ERFTAADLAAVPEESRKQVLGELLYPKVFVREEKLSGKITGMLLEMPNSELLELLEDDSA 629
Query: 223 LKAKVDEAVAVL 258
L +V+EA+ VL
Sbjct: 630 LNERVNEAIGVL 641
>SPBC947.12 |kms2||spindle pole body protein
Kms2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 457
Score = 30.3 bits (65), Expect = 0.18
Identities = 14/43 (32%), Positives = 21/43 (48%)
Frame = -2
Query: 253 QQQLHLL*LSETLHVLTYEEVLNYQFPKAYQLFSQPNQGAFSE 125
QQQ H++ + E +H + E + K L Q +GAF E
Sbjct: 249 QQQAHIVRMEELIHAVEKERKTETDYMKKESLSEQKERGAFME 291
>SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 556
Score = 27.9 bits (59), Expect = 0.97
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Frame = +1
Query: 64 LAAAPLQEQKQMLGERLF-PLIQRMHPDLAGKITGMLLEIDNSELLHMLEHAESLKAKVD 240
L +A +Q+ +M G+ F P ++R H D GK + + LLH L + K++
Sbjct: 290 LESAQVQKVSRMYGQENFQPSMERFHVDPTGKYIALEGRSGHINLLHALTGQFATSFKIE 349
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 26.2 bits (55), Expect = 3.0
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = +1
Query: 124 IQRMHPDLAGKITGMLLEID-NSELLHMLEHAESLKAKVDEAV 249
+QR++P ++ G LL+ID + EL+ L + + VDE V
Sbjct: 801 VQRINPSKTPQVVGALLDIDCDEELVQNLLMSVVGQVPVDELV 843
>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 25.0 bits (52), Expect = 6.8
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 12 STTSSAYPRTRTSDLYYACSSTTP 83
S TSS+ P++RTS+L S TP
Sbjct: 88 SATSSSVPQSRTSELSSTNSIPTP 111
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/29 (34%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +3
Query: 303 IRKASWHQ-EVVSCFNSVTLLSICFIFFD 386
+ K S H+ E ++C ++ L+++C IF D
Sbjct: 532 LMKLSTHKNERIACLSASQLITVCHIFSD 560
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,883,268
Number of Sequences: 5004
Number of extensions: 33062
Number of successful extensions: 136
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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