BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS329B04f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G9.11 |syb1||synaptobrevin |Schizosaccharomyces pombe|chr 1... 28 0.73
SPAPB8E5.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 0.73
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 28 0.97
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 28 0.97
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 26 3.0
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 25 5.2
SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces po... 25 6.8
SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT |Schizosa... 25 6.8
SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 25 9.0
SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr 2... 25 9.0
SPCC417.02 |dad5|hsk3, hos3|DASH complex subunit Dad5|Schizosacc... 25 9.0
>SPAC6G9.11 |syb1||synaptobrevin |Schizosaccharomyces pombe|chr
1|||Manual
Length = 121
Score = 28.3 bits (60), Expect = 0.73
Identities = 21/63 (33%), Positives = 36/63 (57%), Gaps = 5/63 (7%)
Frame = +3
Query: 336 PAEPYNLRVYSPNTHSSTDTNSILASIEERXR-----VLETVSAVQAKQTRRLDAIQDKL 500
PAEP + V S N +S+ N A+I+++ + E +S V +++ RLD++QDK
Sbjct: 10 PAEP-SAAVRSGNAAASSTPNMKTAAIQQQIDDTVGIMRENISKV-SERGERLDSLQDKT 67
Query: 501 DRV 509
D +
Sbjct: 68 DNL 70
>SPAPB8E5.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 103
Score = 28.3 bits (60), Expect = 0.73
Identities = 10/35 (28%), Positives = 22/35 (62%)
Frame = -2
Query: 274 SIGIFQARRFSPFLLLFYWHCFFVISPVLFNFATV 170
SIG+ +FS ++ ++++ F+ SP LF + ++
Sbjct: 68 SIGLSNPNKFSIYIYIYFFFYSFLCSPYLFKYISL 102
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 0.97
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -1
Query: 164 ARASCGYTFAIHACDRCGC 108
A A+C Y F +H DR GC
Sbjct: 294 AAATCKYVFDLHPTDRMGC 312
>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
Ste6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 27.9 bits (59), Expect = 0.97
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = -3
Query: 234 CYCFIGTVFLLFHRYYLTSRRFLSTCVVW 148
C G + + H +Y S+RFL ++W
Sbjct: 544 CSLNFGKISFISHEFYRVSKRFLDILLIW 572
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 26.2 bits (55), Expect = 3.0
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = +3
Query: 357 RVYSPNTHSSTDTNSILASIEERXRVLETVSAVQAKQTRRLDAIQDKLD 503
R S N + + D N++ A E ++LE + K + R + QD+LD
Sbjct: 99 RAPSQNAYEA-DKNALDAKFLEAHKILENLDEQTRKLSERTEKQQDELD 146
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 25.4 bits (53), Expect = 5.2
Identities = 18/48 (37%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Frame = +2
Query: 200 NNKKTVPIKQ*QERAKSTCLEYPYRQGDVKCGWGGAGV--PLFGAHLT 337
N+ K+V K ++ LEY D+K GG GV L G HLT
Sbjct: 1127 NSVKSVHPKISRKLILVATLEYDIPDWDIKIKIGGLGVMAELMGKHLT 1174
>SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1076
Score = 25.0 bits (52), Expect = 6.8
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = -2
Query: 337 GEVRAKQRHASTAPATLNIALSIGIFQARRFS 242
G + +RH++ +P+T+N S QA FS
Sbjct: 659 GTIDLTERHSTASPSTVNSGFSTPRSQATGFS 690
>SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 25.0 bits (52), Expect = 6.8
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +1
Query: 295 LGRCWRAFVW 324
LGRCWR +W
Sbjct: 218 LGRCWRGLLW 227
>SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 604
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +2
Query: 266 PYRQGDVKCGWGGAGVPLFGAHL 334
P+R GD KCG G G F ++
Sbjct: 443 PFRAGDWKCGSEGCGYHNFAKNV 465
>SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 479
Score = 24.6 bits (51), Expect = 9.0
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +3
Query: 366 SPNTHSSTDTNSILASIEERXRVLETVSAVQAKQTRRLDAIQDKL 500
SP T +S D N + SIE R + +S RR+ I+D L
Sbjct: 134 SPGTDTS-DANLTIQSIEAPRRAPQRLSDFFTPDGRRVSNIRDSL 177
>SPCC417.02 |dad5|hsk3, hos3|DASH complex subunit
Dad5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 94
Score = 24.6 bits (51), Expect = 9.0
Identities = 19/58 (32%), Positives = 27/58 (46%)
Frame = +3
Query: 327 RTSPAEPYNLRVYSPNTHSSTDTNSILASIEERXRVLETVSAVQAKQTRRLDAIQDKL 500
RTS + P ++ S + D S L S V+A+QA+ RRL A+Q L
Sbjct: 11 RTSSSSPSPSQMKSFQMNRLVDQLSKLQSNMSHLENHLHVTAIQAEAIRRLGALQASL 68
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,680,067
Number of Sequences: 5004
Number of extensions: 32816
Number of successful extensions: 105
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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