Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS329B02f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 34 0.011
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 30 0.18
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.56
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 28 0.73
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 28 0.97
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 27 1.7
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 26 3.0
SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||... 26 3.9
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 26 3.9
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 25 6.8
SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb... 25 6.8
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 25 6.8
SPBC1703.02 |rsc9||RSC complex subunit Rsc9|Schizosaccharomyces ... 25 6.8
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 25 6.8
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 25 9.0
SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 25 9.0
SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 25 9.0
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 34.3 bits (75), Expect = 0.011
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 414 INLPLFLECGHTVCENCIRNIVKFHEPIECRICQ 515
I P +CGHT C CI + +K + C IC+
Sbjct: 205 ITTPYTTDCGHTYCYACIMSRLKLVNNVSCPICK 238
>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 30.3 bits (65), Expect = 0.18
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +3
Query: 423 PLFLECGHTVCENCIRNIVKFHEPIECRICQ 515
PL C HT C CIR+ ++ H P+ C C+
Sbjct: 39 PLITSCSHTFCSFCIRDYLREH-PM-CPACR 67
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 28.7 bits (61), Expect = 0.56
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 423 PLFLECGHTVCENCIRNIVKFHEPIECRICQK 518
P CGHT C C+ N +K E C C++
Sbjct: 95 PFTTHCGHTYCYECLLNWLK--ESKSCPTCRQ 124
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 28.3 bits (60), Expect = 0.73
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +3
Query: 423 PLFLECGHTVCENCIRN 473
P+ L CG TVC C RN
Sbjct: 29 PVTLPCGGTVCRKCFRN 45
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 27.9 bits (59), Expect = 0.97
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +3
Query: 414 INLPLFLECGHTVCENCIRNIVKFHEPIECRICQ 515
I+ P ECGH C +CI + EC +C+
Sbjct: 263 IHCPAATECGHIFCWSCINGWTS--KKSECPLCR 294
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 27.1 bits (57), Expect = 1.7
Identities = 10/27 (37%), Positives = 12/27 (44%)
Frame = +3
Query: 327 MEPIYLETKKCCPNCSQPYQFQILSTI 407
MEP Y CP C P+ + TI
Sbjct: 664 MEPFYEHKTSTCPQCETPFSVSDILTI 690
Score = 26.2 bits (55), Expect = 3.0
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = +3
Query: 426 LFLECGHTVCENCIRNIVKFHEPIECRICQ 515
L CGH C NC+ + H+ C C+
Sbjct: 651 LIPNCGHAFCSNCMEPFYE-HKTSTCPQCE 679
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 26.2 bits (55), Expect = 3.0
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = +3
Query: 435 ECGHTVCENCIRNIVKFHEPIECRICQK 518
+CGH C C + + K P + C+K
Sbjct: 166 KCGHLFCSTCAKELRKKTVPCPVQHCRK 193
>SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr
2|||Manual
Length = 525
Score = 25.8 bits (54), Expect = 3.9
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +3
Query: 387 FQILSTISKINLPLFLECGHTVCENCI 467
F +LS LPLFLE V + CI
Sbjct: 443 FPLLSNCLLEELPLFLEMARNVTDECI 469
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 25.8 bits (54), Expect = 3.9
Identities = 11/40 (27%), Positives = 19/40 (47%), Gaps = 5/40 (12%)
Frame = +3
Query: 414 INLPLFLECGHTVCENCIRNIVKFHE-----PIECRICQK 518
I PL L C H C +C+ +++ + P C C++
Sbjct: 885 IQNPLLLNCKHACCGDCLSEHIQYQKRRNIIPPLCHTCRQ 924
>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 536
Score = 25.0 bits (52), Expect = 6.8
Identities = 9/28 (32%), Positives = 11/28 (39%)
Frame = +3
Query: 438 CGHTVCENCIRNIVKFHEPIECRICQKC 521
CG +C C+ I P IC C
Sbjct: 300 CGRVICRFCVLEISLPQHPQPLLICMSC 327
>SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 25.0 bits (52), Expect = 6.8
Identities = 6/16 (37%), Positives = 12/16 (75%)
Frame = +3
Query: 450 VCENCIRNIVKFHEPI 497
+C+ C+ I+ F+EP+
Sbjct: 346 ICDQCLEAIIDFYEPV 361
>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 677
Score = 25.0 bits (52), Expect = 6.8
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 423 PLFLECGHTVCENCIRNIVKFHEPIECRICQK 518
P L CGH + +C+RN ++ + C IC++
Sbjct: 323 PKRLPCGHILHFHCLRNWLERQQ--TCPICRR 352
>SPBC1703.02 |rsc9||RSC complex subunit Rsc9|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 780
Score = 25.0 bits (52), Expect = 6.8
Identities = 9/32 (28%), Positives = 20/32 (62%)
Frame = +2
Query: 317 SCKNGTHISGNKKMLSQLQPTLSISNTFNYIK 412
S +N H++ K ++S L+ ++ + TFN ++
Sbjct: 329 SVENAKHVAQTKLVISVLEASIRCAKTFNNLE 360
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 25.0 bits (52), Expect = 6.8
Identities = 8/28 (28%), Positives = 13/28 (46%)
Frame = +3
Query: 432 LECGHTVCENCIRNIVKFHEPIECRICQ 515
L C H +C C + ++ EC C+
Sbjct: 94 LPCNHRMCHVCALRLRALYKTKECTFCK 121
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 24.6 bits (51), Expect = 9.0
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = +3
Query: 432 LECGHTVCENCIRNIVKFHEPIECRICQK 518
+ CGH +NC++ ++ H C +C+K
Sbjct: 125 MPCGHIFGKNCLQKWLENH--CTCPLCRK 151
>SPBC2D10.09 |||3-hydroxyisobutyryl-CoA
hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 429
Score = 24.6 bits (51), Expect = 9.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -1
Query: 329 HFYNFEDFSNYFRL 288
H Y+F+D NYF+L
Sbjct: 387 HEYHFKDLENYFKL 400
>SPBC13E7.02 |cwf24||GCN5-related N
acetyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 533
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = +3
Query: 423 PLFLECGHTVCENCIRNIVKFHEPIECRIC 512
P+ CGH CE C I ++ + C C
Sbjct: 264 PIATTCGHHFCEQCA--ITRYRKTPTCIQC 291
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,262,712
Number of Sequences: 5004
Number of extensions: 46293
Number of successful extensions: 148
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -