Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS327C06f
         (521 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon...    50   2e-07
SPAC4D7.09 |tif223||translation initiation factor eIF2B|Schizosa...    50   3e-07
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual          28   0.73 
SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb...    27   1.3  
SPBC1683.11c |||isocitrate lyase|Schizosaccharomyces pombe|chr 2...    26   3.9  
SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa...    25   5.2  
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    25   9.0  
SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos...    25   9.0  

>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 678

 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 32/126 (25%), Positives = 60/126 (47%), Gaps = 5/126 (3%)
 Frame = +2

Query: 158 QVVVLAAGKGSRMPDVGGSVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVLDEDKSNI 337
           Q +VL+     R   +     +CLLP+   P++ Y    L   G Q+V +         I
Sbjct: 19  QAIVLSDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAGVQEVYVFCCAH-AGQI 77

Query: 338 LNALEKCPLKIK---YELIVIPSEEDWGTANSLKHVSAR--INTDLLVISGDLITNINLN 502
              +EK    +    + +  I S E     ++L+ + ++  I +D +++SGD+++N+ LN
Sbjct: 78  REYIEKSKWNLPSSPFSVNTIVSRESLSVGDALRELDSKQLITSDFILVSGDVVSNVPLN 137

Query: 503 DVLNLH 520
           +VL  H
Sbjct: 138 EVLKEH 143


>SPAC4D7.09 |tif223||translation initiation factor
           eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 468

 Score = 49.6 bits (113), Expect = 3e-07
 Identities = 39/127 (30%), Positives = 68/127 (53%), Gaps = 4/127 (3%)
 Frame = +2

Query: 149 LEFQVVVLAAGKGSRMPDVGG-SVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVLDED 325
           +EFQ VV A    S  P  G  ++ K LLP+G  P+L YPL  LE  GF   +++ ++E 
Sbjct: 37  IEFQAVVFAGFGNSLYPLTGSDALPKALLPIGNKPMLHYPLYWLEAAGFTSAILICMEEA 96

Query: 326 KSNI---LNALEKCPLKIKYELIVIPSEEDWGTANSLKHVSARINTDLLVISGDLITNIN 496
           +++I   L +  +  ++I  E   I  ++   +A++L+ VS       L+ S  ++T+ +
Sbjct: 97  EAHINAWLRSGYEGHMRIHVEAPTI-LDDSKSSADALRAVSH------LIKSQKMLTDTH 149

Query: 497 LNDVLNL 517
            ND + L
Sbjct: 150 QNDFVCL 156


>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 674

 Score = 28.3 bits (60), Expect = 0.73
 Identities = 31/122 (25%), Positives = 54/122 (44%), Gaps = 1/122 (0%)
 Frame = +2

Query: 137 MHKILEFQVVVLAAGKGSRMPDVGGSVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVL 316
           + ++  F V    AG  +R   V   +    L V P+ V+   L+M    G +   ++  
Sbjct: 125 LKRLQRFLVSENEAGNINRQEAVS-MLPPLFLDVEPHHVI---LDMCAAPGSKTAQLIEA 180

Query: 317 DEDKSNILNALEKCPLKIKYELIVIPSEEDWGTANSLKHVSARINT-DLLVISGDLITNI 493
              K+NI +A          E +VI ++ D   A  L H   R+N+ ++LV++ D  T  
Sbjct: 181 VYKKANIKDAAHDSKNLKSVEGLVIANDADPKRAQMLVHQINRLNSPNILVVNHDASTMP 240

Query: 494 NL 499
           N+
Sbjct: 241 NI 242


>SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 450

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
 Frame = +2

Query: 278 EKIGFQDVMIVVLD----EDKSNILNALEKCPLKIKYELIVIPSEED 406
           EK+ +  ++ VVL+    ED +N+ + + KC L I  E + IP+  D
Sbjct: 335 EKVAYGTLVQVVLEDWPLEDFNNLASFMAKCHLPITLEELGIPNVTD 381


>SPBC1683.11c |||isocitrate lyase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 518

 Score = 25.8 bits (54), Expect = 3.9
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +1

Query: 250 STMVSIKYARKNWISR 297
           ST VS+K AR+NWI R
Sbjct: 283 STGVSMKKARENWIER 298


>SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 889

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = -3

Query: 510 STSLRFMLVMRSPDMTSKSVLILALTCF 427
           S S   M V R PD+ S++ ++L L CF
Sbjct: 508 SKSFGRMYVGRYPDVESQNAILLLLGCF 535


>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 10/18 (55%), Positives = 11/18 (61%), Gaps = 2/18 (11%)
 Frame = +1

Query: 271 YARKNWIS--RCNDCGTR 318
           Y    WIS  RCNDC +R
Sbjct: 337 YPYNTWISTIRCNDCNSR 354


>SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm
           type|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 473

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 11/38 (28%), Positives = 19/38 (50%)
 Frame = +2

Query: 404 DWGTANSLKHVSARINTDLLVISGDLITNINLNDVLNL 517
           +W +   L+  S    ++  +  GDL  N+N  DV +L
Sbjct: 168 NWASGGGLREKSISKASEYSIFVGDLSPNVNEFDVYSL 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,102,124
Number of Sequences: 5004
Number of extensions: 43630
Number of successful extensions: 108
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)