BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS325G02f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|S... 157 7e-40
SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch... 149 3e-37
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 31 0.14
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 27 1.3
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.3
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 2.2
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 3.9
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 25 5.2
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 25 5.2
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 25 9.0
SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosacchar... 25 9.0
>SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein
L25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 141
Score = 157 bits (382), Expect = 7e-40
Identities = 75/119 (63%), Positives = 91/119 (76%)
Frame = -2
Query: 403 RKIRNSVHFRRPKTFEPPRHPKYPRKSLPKRNRMDAYNIIKFPLTSEAAMKKIEDNNTLV 224
RK+R S FRRPKT E R PKY RKS+P +R+D Y II P+ SE+AMKKIED+NTLV
Sbjct: 23 RKVRTSTTFRRPKTLELARKPKYARKSVPHASRLDEYKIIVNPINSESAMKKIEDDNTLV 82
Query: 223 FIVHTSANKHHIKAAVKKLYDINVAKVNTLIRPDGKKKAYVRLARDYDALDVANKIGII 47
F VH ANK IK AVKKLY ++ K+NTLIRP+G KKA+V+L+ D DALDVAN+IG +
Sbjct: 83 FHVHLKANKFTIKNAVKKLYSVDAVKINTLIRPNGTKKAFVKLSADADALDVANRIGFL 141
>SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein
L25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 141
Score = 149 bits (360), Expect = 3e-37
Identities = 71/119 (59%), Positives = 88/119 (73%)
Frame = -2
Query: 403 RKIRNSVHFRRPKTFEPPRHPKYPRKSLPKRNRMDAYNIIKFPLTSEAAMKKIEDNNTLV 224
+K+R S FRRPKT + R PKY RKS+ R+D Y II P+ SE+AMKKIED+NTLV
Sbjct: 23 KKVRTSTTFRRPKTLQLSRKPKYARKSVAHAPRLDEYKIIVNPINSESAMKKIEDDNTLV 82
Query: 223 FIVHTSANKHHIKAAVKKLYDINVAKVNTLIRPDGKKKAYVRLARDYDALDVANKIGII 47
F VH ANK IK AV+KLY + K+NTLIRP+G KKA+V+L+ D DALDVAN+IG +
Sbjct: 83 FHVHLKANKFTIKEAVRKLYSVEPVKINTLIRPNGTKKAFVKLSADADALDVANRIGFL 141
>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1826
Score = 30.7 bits (66), Expect = 0.14
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = +2
Query: 32 IWNSLYDANFVGNVQCVIISSESYVRFLLTIRPDECVDF 148
+W +++ A FV + + +S +RFL ++P +C DF
Sbjct: 599 LWITVFIAKFVESYYFLTLSVRDPIRFLQRMKPYDCYDF 637
>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1233
Score = 27.5 bits (58), Expect = 1.3
Identities = 20/83 (24%), Positives = 38/83 (45%)
Frame = -3
Query: 282 NSLSRLKQQ*RRLKITTPWSLLFTQVQTSTISRLQSRNSMI*MLLKSTHSSGLMVRRKRT 103
N L +L+ R +K+T+ T + S+++S + + L T + L+ +
Sbjct: 396 NLLFKLQTLNRNIKVTSQSKDSLTSIVGDLESKIKSLHESVSSL--DTERADLLAKINEK 453
Query: 102 YDSLEIMTHWTLPTKLASYKLFH 34
+SLE+ H +L +LFH
Sbjct: 454 IESLELEKHDQQKKRLTYSELFH 476
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 27.5 bits (58), Expect = 1.3
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +3
Query: 378 KCTELRIFRTLFPCSPFYNLPLSL 449
+C +L+ +T PCS +N PLS+
Sbjct: 261 ECGDLKFLQTDLPCSGSHNYPLSI 284
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 26.6 bits (56), Expect = 2.2
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +2
Query: 74 QCVIISSESYVRFLLTIRPDECVDFSNIY 160
+C+ I E + FL I+P E V F ++
Sbjct: 518 RCIEIGGEQFSGFLTNIKPFEAVTFQKLF 546
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 25.8 bits (54), Expect = 3.9
Identities = 9/51 (17%), Positives = 25/51 (49%)
Frame = -2
Query: 304 MDAYNIIKFPLTSEAAMKKIEDNNTLVFIVHTSANKHHIKAAVKKLYDINV 152
+ A+++ +F E ++ +DN T I H + ++ + A +++ +
Sbjct: 1090 LQAFDLSRFEFIKEIFLELYDDNETNASIAHQISTQNGLDATETSFFELQI 1140
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 25.4 bits (53), Expect = 5.2
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 38 NSLYDANFVGNVQCVIISSESYVRFLLTIRPD 133
++ +NF NV C I +S F IRPD
Sbjct: 866 HTFISSNFFENVPCYISLEQSLQNFQNDIRPD 897
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 25.4 bits (53), Expect = 5.2
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = +2
Query: 17 FYS*KIWNSLYDANFVGNVQCVIISSESYVRFLLTIRPDECVDF 148
F S +W ++ A F+ + + ++ +RFL +RP +C D+
Sbjct: 503 FVSWCLWITVLVAKFLESYFFLTLNLADSIRFLGAMRPYDCRDY 546
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 24.6 bits (51), Expect = 9.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 397 IRNSVHFRRPKTFEPPRHP 341
IR +H+ +PK+ E P P
Sbjct: 43 IRKHIHYPKPKSIEAPMFP 61
>SPBC1718.07c |zfs1|moc4|transcription factor Zfs1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 404
Score = 24.6 bits (51), Expect = 9.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -2
Query: 358 EPPRHPKY 335
EPPRHPKY
Sbjct: 358 EPPRHPKY 365
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,099,494
Number of Sequences: 5004
Number of extensions: 41396
Number of successful extensions: 130
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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