BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS324F09f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 144 7e-36
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 64 9e-12
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 61 9e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 60 2e-10
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 46 3e-06
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 39 5e-04
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 38 0.001
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 38 0.001
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 33 0.034
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 0.97
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 3.9
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 3.9
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 6.8
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 25 9.0
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar... 25 9.0
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 25 9.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 144 bits (349), Expect = 7e-36
Identities = 65/71 (91%), Positives = 69/71 (97%)
Frame = -2
Query: 520 MYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 341
MYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDE
Sbjct: 305 MYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 364
Query: 340 SGPSIVHRKCF 308
SGP IV+RKCF
Sbjct: 365 SGPGIVYRKCF 375
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 64.5 bits (150), Expect = 9e-12
Identities = 32/73 (43%), Positives = 50/73 (68%), Gaps = 6/73 (8%)
Frame = -2
Query: 511 GIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISKQEYDE 341
G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E
Sbjct: 361 GFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQLWISRQEYEE 419
Query: 340 SGP---SIVHRKC 311
G +++ ++C
Sbjct: 420 HGSDRLALIEKRC 432
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 61.3 bits (142), Expect = 9e-11
Identities = 27/68 (39%), Positives = 47/68 (69%)
Frame = -2
Query: 511 GIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP 332
G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++
Sbjct: 313 GFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQ 371
Query: 331 SIVHRKCF 308
+++ R+ F
Sbjct: 372 NVIFRRRF 379
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 60.1 bits (139), Expect = 2e-10
Identities = 29/64 (45%), Positives = 40/64 (62%), Gaps = 2/64 (3%)
Frame = -2
Query: 520 MYPGIADRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSILASLSTFQQMWISKQEY 347
+ PG+++R+Q E+ LA S + + +VW GGSILASL FQ +W+SKQEY
Sbjct: 355 LIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEY 414
Query: 346 DESG 335
DE G
Sbjct: 415 DEVG 418
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 46.0 bits (104), Expect = 3e-06
Identities = 27/74 (36%), Positives = 44/74 (59%), Gaps = 12/74 (16%)
Frame = -2
Query: 520 MYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTF 377
MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA ++
Sbjct: 305 MYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQN 364
Query: 376 QQMWISKQEYDESG 335
MW+SK E++E G
Sbjct: 365 DHMWVSKAEWEEYG 378
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 38.7 bits (86), Expect = 5e-04
Identities = 20/68 (29%), Positives = 35/68 (51%)
Frame = -2
Query: 517 YPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDES 338
+PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E
Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390
Query: 337 GPSIVHRK 314
G +I+ RK
Sbjct: 391 GANIMTRK 398
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 37.9 bits (84), Expect = 0.001
Identities = 16/64 (25%), Positives = 31/64 (48%)
Frame = -2
Query: 514 PGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 335
PG+ R+++E+T++ P I + W G S + F+ ++++EY E G
Sbjct: 648 PGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKG 707
Query: 334 PSIV 323
P +
Sbjct: 708 PEYI 711
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 37.5 bits (83), Expect = 0.001
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -2
Query: 454 IKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 317
+ +I+ ++ +VW GGS+LA F +K +Y+E G SI R
Sbjct: 374 VNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 32.7 bits (71), Expect = 0.034
Identities = 15/71 (21%), Positives = 32/71 (45%), Gaps = 3/71 (4%)
Frame = -2
Query: 517 YPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISKQEY 347
+PG A +++ I + + I +I PP + ++ W G I + ++WI ++
Sbjct: 542 FPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSELWIKNSDW 601
Query: 346 DESGPSIVHRK 314
G ++ K
Sbjct: 602 KMLGSRVLQYK 612
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 0.97
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -2
Query: 415 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 314
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 3.9
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -3
Query: 423 TPYGSVDRSSPPSLPSNRCGSRNRSTTSLAPP 328
TP + PPS+PS+R R S ++ APP
Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 3.9
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 490 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 380
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 25.0 bits (52), Expect = 6.8
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2
Query: 356 FRDPHLLEGREGGEDRSTDPYGVLPLWRS 442
F HLL + G+D + + YG L W S
Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSWIS 825
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 24.6 bits (51), Expect = 9.0
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -2
Query: 388 LSTFQQMWISKQEYDESGPSIV 323
L + + W KQ++DES S+V
Sbjct: 2195 LQKWYEEWCGKQDWDESDKSVV 2216
>SPBC530.14c |dsk1||SR protein-specific kinase
Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 544
Score = 24.6 bits (51), Expect = 9.0
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2
Query: 434 WRSNDLNLHCRWGESCD 484
+RS ++ L CRWG S D
Sbjct: 380 YRSPEVILGCRWGASAD 396
>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 204
Score = 24.6 bits (51), Expect = 9.0
Identities = 19/56 (33%), Positives = 23/56 (41%)
Frame = -3
Query: 420 PYGSVDRSSPPSLPSNRCGSRNRSTTSLAPPLYTGSASKRTARRCLQQPAAGCSIQ 253
P SVD S+PPS P S + S P SA+ A Q A G + Q
Sbjct: 49 PPPSVDHSAPPSGPP---PSYSNSAAPATPAASASSAAPAPAPAASQNRAYGAAPQ 101
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,829,442
Number of Sequences: 5004
Number of extensions: 33345
Number of successful extensions: 99
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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