Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS320E12f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|... 100 1e-22
SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr... 99 5e-22
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 27 1.7
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 1.7
SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosacchar... 26 3.9
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 25 5.2
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce... 25 5.2
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 6.8
>SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|chr
3|||Manual
Length = 542
Score = 100 bits (240), Expect = 1e-22
Identities = 50/149 (33%), Positives = 86/149 (57%), Gaps = 7/149 (4%)
Frame = +3
Query: 3 SNVFAGTAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGT--PEQLDAML 176
SN+ AG + QIQ I++ ++ P+ +L D K QKEA WAI+N GG P+Q+ ++
Sbjct: 357 SNITAGNSSQIQYVIEANIIPPLIHLLTTADFKIQKEACWAISNATSGGARRPDQIRYLV 416
Query: 177 SCDILSPYCALLQARDHRAIIVVLDGLTHLLQA-----AAKYDQVRGMCIKLEEIGALDQ 341
+ P C LL +D++ I V LDG+ ++L+ A D++ + +E+ G +D
Sbjct: 417 EQGAIKPLCNLLACQDNKIIQVALDGIENILRVGELDRANNPDKINLYAVYVEDAGGMDL 476
Query: 342 IENLQQHENEQIYKKSIHILDSYFMEEED 428
I Q N +IY+K+ +I++ +F EE++
Sbjct: 477 IHECQNSSNSEIYQKAYNIIEKFFGEEDE 505
Score = 40.7 bits (91), Expect = 1e-04
Identities = 20/68 (29%), Positives = 37/68 (54%)
Frame = +3
Query: 21 TAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPY 200
T P IQ+ ID+GV+ L H + + EA+WA+TN+ G+ Q ++ + + +
Sbjct: 110 TNPPIQKVIDAGVVPRFVEFLSHENNLLKFEASWALTNVA-SGSSNQTHVVVEANAVPVF 168
Query: 201 CALLQARD 224
+LL + +
Sbjct: 169 VSLLSSSE 176
Score = 40.3 bits (90), Expect = 2e-04
Identities = 35/148 (23%), Positives = 67/148 (45%), Gaps = 2/148 (1%)
Frame = +3
Query: 6 NVFAGTAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGTPEQLDAMLSCD 185
N+ G Q Q I+ G L + +L +KEA W I+N+ G + Q+ ++ +
Sbjct: 316 NIVTGDDVQTQVIINCGALSALLSLLSSPRDGVRKEACWTISNITAGNS-SQIQYVIEAN 374
Query: 186 ILSPYCALLQARDHRAIIVVLDGLTHLLQAAAKY-DQVRGMCIKLEEIGALDQIENLQQH 362
I+ P LL D + +++ A+ DQ+R L E GA+ + NL
Sbjct: 375 IIPPLIHLLTTADFKIQKEACWAISNATSGGARRPDQIR----YLVEQGAIKPLCNLLAC 430
Query: 363 ENEQIYKKSIHILDSYF-MEEEDASTQP 443
++ +I + ++ +++ + E D + P
Sbjct: 431 QDNKIIQVALDGIENILRVGELDRANNP 458
Score = 33.5 bits (73), Expect = 0.020
Identities = 20/90 (22%), Positives = 41/90 (45%), Gaps = 2/90 (2%)
Frame = +3
Query: 15 AGTAPQIQ-RAIDSGVLEPIARVLV-HHDIKCQKEAAWAITNLCLGGTPEQLDAMLSCDI 188
AG +P + + GVLEP+ ++ + + + + W ++N+C G P Q D +
Sbjct: 191 AGDSPMCRDHVLQCGVLEPLLNIIESNRRLSMLRNSTWTLSNMCRGKNP-QPDWNSISQV 249
Query: 189 LSPYCALLQARDHRAIIVVLDGLTHLLQAA 278
+ L+ D ++ L +++L A
Sbjct: 250 IPVLSKLIYTLDEDVLVDALWAISYLSDGA 279
Score = 33.1 bits (72), Expect = 0.026
Identities = 18/67 (26%), Positives = 34/67 (50%)
Frame = +3
Query: 18 GTAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGTPEQLDAMLSCDILSP 197
G +IQ ID+G+ + +L+H + Q A ++ N+ + G Q +++C LS
Sbjct: 278 GANEKIQAIIDAGIPRRLVELLMHPSAQVQTPALRSVGNI-VTGDDVQTQVIINCGALSA 336
Query: 198 YCALLQA 218
+LL +
Sbjct: 337 LLSLLSS 343
>SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr
2|||Manual
Length = 539
Score = 98.7 bits (235), Expect = 5e-22
Identities = 54/163 (33%), Positives = 87/163 (53%), Gaps = 7/163 (4%)
Frame = +3
Query: 3 SNVFAGTAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLG--GTPEQLDAML 176
SN+ AG QIQ I+S ++ P+ +L + D K +KEA WAI+N G G P+Q+ ++
Sbjct: 357 SNITAGNTQQIQAIIESNLIPPLVHLLSYADYKTKKEACWAISNATSGGLGQPDQIRYLV 416
Query: 177 SCDILSPYCALLQARDHRAIIVVLDGLTHLLQA-----AAKYDQVRGMCIKLEEIGALDQ 341
S ++ P C +L D++ I V LD + ++L+ + + + +EE G +D
Sbjct: 417 SQGVIKPLCDMLNGSDNKIIQVALDAIENILKVGEMDRTMDLENINQYAVYVEEAGGMDM 476
Query: 342 IENLQQHENEQIYKKSIHILDSYFMEEEDASTQPAHDHEQYQF 470
I +LQ N IY K+ I++ YF +EDA A + E F
Sbjct: 477 IHDLQSSGNNDIYLKAYSIIEKYF-SDEDAVEDLAPETENGAF 518
Score = 51.2 bits (117), Expect = 9e-08
Identities = 25/75 (33%), Positives = 39/75 (52%)
Frame = +3
Query: 6 NVFAGTAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGTPEQLDAMLSCD 185
N+ GT Q Q ID G L +L H +KEA W I+N+ G T +Q+ A++ +
Sbjct: 316 NIVTGTDAQTQIIIDCGALNAFPSLLSHQKENIRKEACWTISNITAGNT-QQIQAIIESN 374
Query: 186 ILSPYCALLQARDHR 230
++ P LL D++
Sbjct: 375 LIPPLVHLLSYADYK 389
Score = 35.1 bits (77), Expect = 0.006
Identities = 18/72 (25%), Positives = 37/72 (51%)
Frame = +3
Query: 3 SNVFAGTAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGTPEQLDAMLSC 182
+N+ +GT Q + +DSG + ++L + +++ WA+ N+ G + D +L
Sbjct: 145 TNIASGTTDQTRIVVDSGAVPRFIQLLSSPEKDVREQVVWALGNIA-GDSSACRDYVLGN 203
Query: 183 DILSPYCALLQA 218
+L P +LQ+
Sbjct: 204 GVLQPLLNILQS 215
Score = 33.9 bits (74), Expect = 0.015
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +3
Query: 21 TAPQIQRAIDSGVLEPIARVLVHHDIKCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPY 200
T P I + I GV++ + L Q EAAWA+TN+ GT +Q ++ + +
Sbjct: 109 THPPIDQVIACGVVDRFVQFLESEHHLLQFEAAWALTNIA-SGTTDQTRIVVDSGAVPRF 167
Query: 201 CALLQA 218
LL +
Sbjct: 168 IQLLSS 173
Score = 32.3 bits (70), Expect = 0.045
Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +3
Query: 45 IDSGVLEPIARVLVHH--DIKCQKEAAWAITNLCLGGTP 155
+ +GVL+P+ +L D+ + A W ++NLC G P
Sbjct: 201 LGNGVLQPLLNILQSSASDVSMLRNATWTLSNLCRGKNP 239
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 27.1 bits (57), Expect = 1.7
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 2/60 (3%)
Frame = +3
Query: 99 KCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLDG--LTHLLQ 272
K KE I N P+ A + CD+ +CA + DH ++V+ +G + H+L+
Sbjct: 54 KYDKEEFSVIWNEVSMEFPDIRRAKVFCDLDLEFCAQQEIYDHPKVVVLKNGMWMRHVLE 113
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.1 bits (57), Expect = 1.7
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +1
Query: 382 RRASTSWTPTLWRRRTPARSPLTTTSSISSGQ 477
+ ASTS + TPA S TTTSS+SS Q
Sbjct: 734 KTASTSSGSSSSSSYTPASSTSTTTSSVSSRQ 765
>SPAC57A7.10c |sec21||coatomer gamma subunit Sec21
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.8 bits (54), Expect = 3.9
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = +3
Query: 15 AGTAPQIQRAIDSGVLEPIARV 80
A T P I+R + +G+++PI+ V
Sbjct: 131 ANTVPAIERILTTGIVDPISAV 152
>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1473
Score = 25.4 bits (53), Expect = 5.2
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +3
Query: 393 HILDSYFMEEEDASTQP 443
HI D+ EEE ASTQP
Sbjct: 73 HIADAILAEEEVASTQP 89
>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1261
Score = 25.4 bits (53), Expect = 5.2
Identities = 13/35 (37%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +3
Query: 81 LVHHDIKCQKEA-AWAITNLCLGGTPEQLDAMLSC 182
L++HD+ + A I ++ L GT QLD++L C
Sbjct: 320 LINHDLIYSPQFYAILIESVSLYGTQSQLDSLLEC 354
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 25.0 bits (52), Expect = 6.8
Identities = 8/27 (29%), Positives = 18/27 (66%)
Frame = +3
Query: 18 GTAPQIQRAIDSGVLEPIARVLVHHDI 98
G+ +++ IDS ++ +A +L HH++
Sbjct: 514 GSVVELRNLIDSSLISSLAFLLEHHEV 540
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,202,547
Number of Sequences: 5004
Number of extensions: 44893
Number of successful extensions: 160
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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