Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS319C11f
         (521 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   259   2e-70
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   254   4e-69
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   103   2e-23
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    53   2e-08
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    31   0.10 
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl...    28   0.73 
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch...    26   3.0  
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    26   3.9  
SPAC30D11.11 |||Haemolysin-III family protein|Schizosaccharomyce...    25   5.2  
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    25   5.2  
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|...    25   5.2  
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    25   9.0  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  259 bits (634), Expect = 2e-70
 Identities = 113/138 (81%), Positives = 127/138 (92%)
 Frame = -1

Query: 521 MVKCDPRHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 342
           MVKCDPR G+YMA C+LYRGDV+PRDV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP 
Sbjct: 306 MVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQ 365

Query: 341 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 162
            VPG  +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEA
Sbjct: 366 HVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEA 425

Query: 161 REDLAALEKDYEEVGMDS 108
           REDLAALE+DYEEVG DS
Sbjct: 426 REDLAALERDYEEVGQDS 443


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  254 bits (623), Expect = 4e-69
 Identities = 112/138 (81%), Positives = 125/138 (90%)
 Frame = -1

Query: 521 MVKCDPRHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 342
           MVKCDPR G+YMA C+LYRGDV+PRDV AA+ TIK KRTIQFVDWCPTGFK+GI  +PP 
Sbjct: 302 MVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQ 361

Query: 341 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 162
            + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEA
Sbjct: 362 HIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEA 421

Query: 161 REDLAALEKDYEEVGMDS 108
           REDLAALE+DYEEVG DS
Sbjct: 422 REDLAALERDYEEVGQDS 439


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  103 bits (247), Expect = 2e-23
 Identities = 52/140 (37%), Positives = 81/140 (57%), Gaps = 3/140 (2%)
 Frame = -1

Query: 521 MVKCDPRHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 342
           MV  DPRHG+Y+    L+RG V  ++V+  I +++TK +  FV+W P      +   PP 
Sbjct: 300 MVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK 359

Query: 341 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 162
                DL   + +   + N+T+I E + RL  +F  M+ ++AF+HWY GEGM+E EF+EA
Sbjct: 360 -----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEA 411

Query: 161 R---EDLAALEKDYEEVGMD 111
                DL +  + Y+E G+D
Sbjct: 412 ESNMNDLVSEYQQYQEAGID 431


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 53.2 bits (122), Expect = 2e-08
 Identities = 34/136 (25%), Positives = 69/136 (50%), Gaps = 4/136 (2%)
 Frame = -1

Query: 521 MVKCDP-RHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 345
           MV  +P +   +++   + +G+  P DV+ ++  I+ +R   F+ W P   +V ++ + P
Sbjct: 306 MVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSP 365

Query: 344 TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GE 174
            +     ++ +     ML+N T+IA  + R   ++D +  + AF+  Y  E + E    E
Sbjct: 366 YIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNE 420

Query: 173 FSEAREDLAALEKDYE 126
           F  +R+ +A L  +YE
Sbjct: 421 FDSSRDVVADLINEYE 436


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 16/45 (35%), Positives = 21/45 (46%)
 Frame = -2

Query: 466 VVTSYPGM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 332
           VV   P +  RP++P  P  LS     V PV+  V +   PP  P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596



 Score = 25.4 bits (53), Expect = 5.2
 Identities = 14/45 (31%), Positives = 18/45 (40%)
 Frame = -2

Query: 466 VVTSYPGM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 332
           VV   P +  RP++P  P   S       PV   V +   PP  P
Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686


>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
           subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 709

 Score = 28.3 bits (60), Expect = 0.73
 Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
 Frame = -2

Query: 262 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 89
           G+   TS TS T S       S++     S+P P   W P+        S+ TP+   V 
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207

Query: 88  EPKSTK 71
           EP+ TK
Sbjct: 208 EPRFTK 213


>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 628

 Score = 26.2 bits (55), Expect = 3.0
 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
 Frame = -2

Query: 430 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 344
           +LP KPS+   +++S   V+P S   STTS+P
Sbjct: 5   TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 25.8 bits (54), Expect = 3.9
 Identities = 10/20 (50%), Positives = 11/20 (55%)
 Frame = +2

Query: 449 WVRRHHGTAYSKPCTCHDGG 508
           W R H    Y+KPC   DGG
Sbjct: 16  WRRDHPFGFYAKPCKSSDGG 35


>SPAC30D11.11 |||Haemolysin-III family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 10/27 (37%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
 Frame = +3

Query: 444 IPG-YDVTTVQHTASHVLAMTGVAFHH 521
           +PG +D+    H   H+  + GVAFH+
Sbjct: 398 LPGKFDIIGNSHQIWHIAIIVGVAFHY 424


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
 Frame = -2

Query: 433 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 338
           P  PS+P+++SN ST  G+Q V   V   +   W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564


>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 530

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 19/60 (31%), Positives = 26/60 (43%)
 Frame = -2

Query: 511 ATPVMASTWLAVCCTVVTSYPGM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 332
           +TP ++S   A    VV+S     T  +  S  S  ++SS    P S SVS      W P
Sbjct: 245 STPAVSSA-AASSSAVVSSVVSSATSVAASSTISSATSSSASASPTSSSVSGKRGLAWIP 303


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
            Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 9/24 (37%), Positives = 13/24 (54%)
 Frame = -1

Query: 299  CMLSNTTAIAEAWARLDHKFDLMY 228
            C++      +  W RLD+ F LMY
Sbjct: 1336 CVIVEMATGSPPWPRLDNHFSLMY 1359


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,195,369
Number of Sequences: 5004
Number of extensions: 44584
Number of successful extensions: 172
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)