Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS315B09f
(310 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 161 3e-41
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 160 4e-41
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 160 4e-41
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 98 2e-22
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 91 3e-20
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 61 4e-11
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 29 0.21
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 24 4.5
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 24 5.9
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 24 5.9
SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyce... 24 5.9
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 23 7.9
SPAC6F6.09 |||NuA4 histone acetyltransferase complex subunit |Sc... 23 7.9
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 23 7.9
SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|ch... 23 7.9
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 161 bits (390), Expect = 3e-41
Identities = 77/103 (74%), Positives = 89/103 (86%)
Frame = +2
Query: 2 IKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDST 181
IKNMITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T
Sbjct: 99 IKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT 158
Query: 182 EPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVXISGWHGDNM 310
+S+ RFEEI KE S++IKK+G+NP V FV +SG+ GDNM
Sbjct: 159 --GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNM 199
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 160 bits (389), Expect = 4e-41
Identities = 76/103 (73%), Positives = 89/103 (86%)
Frame = +2
Query: 2 IKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDST 181
IKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T
Sbjct: 99 IKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT 158
Query: 182 EPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVXISGWHGDNM 310
+S+ RFEEI KE S++IKK+G+NP V FV +SG+ GDNM
Sbjct: 159 --GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNM 199
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 160 bits (389), Expect = 4e-41
Identities = 76/103 (73%), Positives = 89/103 (86%)
Frame = +2
Query: 2 IKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDST 181
IKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T
Sbjct: 99 IKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT 158
Query: 182 EPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVXISGWHGDNM 310
+S+ RFEEI KE S++IKK+G+NP V FV +SG+ GDNM
Sbjct: 159 --GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNM 199
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 98.3 bits (234), Expect = 2e-22
Identities = 46/105 (43%), Positives = 70/105 (66%), Gaps = 2/105 (1%)
Frame = +2
Query: 2 IKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDST 181
+ NMI G SQAD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD
Sbjct: 330 VTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEP 389
Query: 182 EPPYSEPRFEEIKKEVSSYIKKI-GYNPAA-VAFVXISGWHGDNM 310
+SE R++E ++S +++++ GYN V ++ +S + G N+
Sbjct: 390 SVQWSEERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNV 434
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 91.5 bits (217), Expect = 3e-20
Identities = 49/104 (47%), Positives = 64/104 (61%), Gaps = 1/104 (0%)
Frame = +2
Query: 2 IKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDST 181
I MI G S AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D
Sbjct: 269 ISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLM 328
Query: 182 EPPYSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVXISGWHGDNM 310
+SE RF+EIK VS + IK +G+ + V FV IS G N+
Sbjct: 329 S--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNL 370
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 60.9 bits (141), Expect = 4e-11
Identities = 34/79 (43%), Positives = 49/79 (62%)
Frame = +2
Query: 2 IKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDST 181
IKNMITG + D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D
Sbjct: 130 IKNMITGAATMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMV 182
Query: 182 EPPYSEPRFEEIKKEVSSY 238
EP E E+++ +S Y
Sbjct: 183 EPDMIELVEMEMRELLSEY 201
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 28.7 bits (61), Expect = 0.21
Identities = 18/77 (23%), Positives = 33/77 (42%)
Frame = +2
Query: 11 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 190
M+ G + D A+L++A QT EH + +K +I+ NK+D
Sbjct: 125 MLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQLKHIIILQNKVDLIRES 178
Query: 191 YSEPRFEEIKKEVSSYI 241
+E ++ I K + +
Sbjct: 179 AAEEHYQSILKFIKGTV 195
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 24.2 bits (50), Expect = 4.5
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = -2
Query: 135 RVKASKACSRV*PFLEIPASNSPVP 61
R S +C+R+ P + +P S P P
Sbjct: 601 RTLQSLSCARIRPLVMVPKSKKPSP 625
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 23.8 bits (49), Expect = 5.9
Identities = 13/39 (33%), Positives = 16/39 (41%)
Frame = +2
Query: 185 PPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVXISGWHG 301
P + P EVS I +P AFV + G HG
Sbjct: 1558 PVFVTPPTNSPYAEVSGDYNPIHVSPTFAAFVELPGTHG 1596
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 23.8 bits (49), Expect = 5.9
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = -1
Query: 229 YFLLDFLKSGLTVWWFSG 176
Y +L + G+ VW+FSG
Sbjct: 679 YMVLGIVTVGILVWYFSG 696
>SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 213
Score = 23.8 bits (49), Expect = 5.9
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -1
Query: 277 ESDSSWVVANLLDV*GYFLL 218
E+D+ W V +D+ G FLL
Sbjct: 2 ENDTGWSVKKCIDIFGEFLL 21
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 23.4 bits (48), Expect = 7.9
Identities = 9/41 (21%), Positives = 18/41 (43%)
Frame = +2
Query: 173 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVXISGW 295
DS P + + + + YI+++G P + I+ W
Sbjct: 513 DSFNTPLVMQHIDNLVTQANIYIREVGQQPCSRLLGQIASW 553
>SPAC6F6.09 |||NuA4 histone acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 138
Score = 23.4 bits (48), Expect = 7.9
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +2
Query: 179 TEPPYSEPRFEEIKKEVSSYIKK 247
+EPP + +E+ KKE+ I+K
Sbjct: 9 SEPPVNVSYYEQCKKELHEMIEK 31
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 23.4 bits (48), Expect = 7.9
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 231 PHTSRRLATTQLLSLSXPFL 290
PH RL ++L LS PFL
Sbjct: 675 PHDKLRLYALKVLKLSVPFL 694
>SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 23.4 bits (48), Expect = 7.9
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -2
Query: 219 LISSNLGSLYGGSVESI 169
+I S+LGSL GG+V S+
Sbjct: 395 IIGSSLGSLLGGAVISL 411
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,312,533
Number of Sequences: 5004
Number of extensions: 24164
Number of successful extensions: 91
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 86
length of database: 2,362,478
effective HSP length: 63
effective length of database: 2,047,226
effective search space used: 79841814
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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