Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS314H10f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family pr... 71 4e-13
At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family pr... 53 1e-07
At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolas... 53 1e-07
At1g60550.1 68414.m06816 naphthoate synthase, putative / dihydro... 49 2e-06
At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolas... 48 4e-06
At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family pr... 47 7e-06
At3g60510.1 68416.m06768 enoyl-CoA hydratase/isomerase family pr... 46 2e-05
At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family pr... 45 4e-05
At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family pr... 44 5e-05
At4g13360.1 68417.m02089 enoyl-CoA hydratase/isomerase family pr... 43 1e-04
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 43 1e-04
At5g56110.1 68418.m07000 myb family transcription factor contain... 30 1.1
At4g15820.1 68417.m02407 wound-responsive protein-related contai... 28 3.3
At5g05970.1 68418.m00661 transducin family protein / WD-40 repea... 28 4.4
At2g34750.1 68415.m04267 RNA polymerase I specific transcription... 27 5.8
At4g35180.1 68417.m05001 amino acid transporter family protein s... 27 7.7
At3g28360.1 68416.m03544 ABC transporter family protein similar ... 27 7.7
At2g39960.1 68415.m04910 microsomal signal peptidase 25 kDa subu... 27 7.7
>At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family
protein similar to 3-hydroxybutyryl-CoA dehydratase
(Crotonase) from Clostridium acetobutylicum [SP|P52046],
FadB1x (enoyl-CoA hydratase) from Pseudomonas putida
[GI:13310130]; contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 265
Score = 71.3 bits (167), Expect = 4e-13
Identities = 34/105 (32%), Positives = 60/105 (57%)
Frame = +3
Query: 207 ENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGNEKA 386
EN+ ++V + +I +NRPK+LN+L + + V+L KA D D+D ++ +I TG+ ++
Sbjct: 7 ENL-IQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRS 65
Query: 387 FAAGADIKEMQNNTYSSNTKQGFLREWEDISNCGKPIIAAVNGFA 521
F +G D+ + + + + K + KPII A+NGFA
Sbjct: 66 FCSGVDLTAAE-SVFKGDVKDPETDPVVQMERLRKPIIGAINGFA 109
>At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family
protein similar to Delta 3,5-delta2,4-dienoyl-CoA
isomerase, mitochondrial (ECH1) from Rattus norvegicus
[SP|Q62651], from Homo sapiens [SP|Q13011]; contains
Pfam profile PF00378 enoyl-CoA hydratase/isomerase
family protein
Length = 278
Score = 53.2 bits (122), Expect = 1e-07
Identities = 33/119 (27%), Positives = 60/119 (50%), Gaps = 14/119 (11%)
Frame = +3
Query: 201 SYENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGNE 380
SY+ +++ + +V + +NRP LNAL F+E KA++ D + +++ II++G
Sbjct: 5 SYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGAG 64
Query: 381 KAFAAGADIKEMQNNTYSSNTKQGFLREWED--------------ISNCGKPIIAAVNG 515
K F +G D+ + + + S++ R E I C KP+IAA++G
Sbjct: 65 KHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHG 123
>At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 378
Score = 53.2 bits (122), Expect = 1e-07
Identities = 30/103 (29%), Positives = 52/103 (50%), Gaps = 6/103 (5%)
Frame = +3
Query: 225 VVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGNEKAFAAGAD 404
+V K +V ++ LNRPK LNALC + L + ++ D ++ +I+ G +AF AG D
Sbjct: 8 LVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAGGD 67
Query: 405 IKEMQNNTYSSNTKQG--FLREWEDI----SNCGKPIIAAVNG 515
+ + N + G F R+ + + KP ++ +NG
Sbjct: 68 VPPVVQNMVQGKWRLGADFFRDQYTLNYVMATYSKPQVSILNG 110
>At1g60550.1 68414.m06816 naphthoate synthase, putative /
dihydroxynaphthoic acid synthetase, putative / DHNA
synthetase, putative contains similarity to MENB from
Escherichia coli [SP|P27290], Bacillus subtilis
[SP|P23966]; contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 337
Score = 49.2 bits (112), Expect = 2e-06
Identities = 33/98 (33%), Positives = 47/98 (47%), Gaps = 4/98 (4%)
Frame = +3
Query: 240 KNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITG-NEKAFAAGADIKEM 416
+ + I +NRP+ NA EL +A ND DS++ II+TG KAF +G D
Sbjct: 85 EGIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALR 144
Query: 417 QNNTYSSNTKQGFLREWE---DISNCGKPIIAAVNGFA 521
+ Y+ G L + I KP+IA V G+A
Sbjct: 145 TQDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYA 182
>At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolase /
CoA-thioester hydrolase (CHY1) identical to gi:8572760;
contains Pfam profile PF00388 enoyl-CoA
hydratase/isomerase family protein
Length = 378
Score = 48.0 bits (109), Expect = 4e-06
Identities = 24/76 (31%), Positives = 41/76 (53%)
Frame = +3
Query: 225 VVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGNEKAFAAGAD 404
+V K +V ++ LNRPK LNAL + L + F+ D ++ +I+ G+ +AF AG D
Sbjct: 12 LVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAGGD 71
Query: 405 IKEMQNNTYSSNTKQG 452
+ + + N + G
Sbjct: 72 VAAVVRDINQGNWRLG 87
>At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 409
Score = 47.2 bits (107), Expect = 7e-06
Identities = 27/89 (30%), Positives = 49/89 (55%)
Frame = +3
Query: 192 STASYENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIIT 371
S A +E+ +V V G K+ I LN P +LNAL P+ L + ++ + I+ +++
Sbjct: 35 SDADFED-QVLVEGKAKSRAAI-LNNPSSLNALSAPMVGRLKRLYESWEENPAISFVLMK 92
Query: 372 GNEKAFAAGADIKEMQNNTYSSNTKQGFL 458
G+ K F +GAD+ + ++ NT++ L
Sbjct: 93 GSGKTFCSGADVLSLYHSINEGNTEESKL 121
>At3g60510.1 68416.m06768 enoyl-CoA hydratase/isomerase family
protein similar to enoyl-CoA-hydratase, Avicennia
marina, EMBL:AF190450 [GI:6014701], CoA-thioester
hydrolase CHY1 from Arabidopsis thaliana [GI:8572760];
contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 401
Score = 45.6 bits (103), Expect = 2e-05
Identities = 33/99 (33%), Positives = 49/99 (49%), Gaps = 1/99 (1%)
Frame = +3
Query: 114 RALLGKNVLNKC-KVVSATSQASIKFYSTASYENIKVEVVGSKKNVGLIQLNRPKALNAL 290
+ LLG+ V +K + S S+K S + VE G + LNRP ALNAL
Sbjct: 5 KGLLGRIVRDKLWRFGYRRSLCSLKLTSEDLDYQVLVEGSGCSRTA---ILNRPPALNAL 61
Query: 291 CKPLFVELGKAVNDFDADSNIAAIIITGNEKAFAAGADI 407
+ L K +++ D NI +++ G+ +AF AG DI
Sbjct: 62 TTHMGYRLQKLYKNWEEDPNIGFVMMKGSGRAFCAGGDI 100
>At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 418
Score = 44.8 bits (101), Expect = 4e-05
Identities = 34/128 (26%), Positives = 67/128 (52%), Gaps = 8/128 (6%)
Frame = +3
Query: 156 VSATSQASIKFYSTASYENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDF 335
+S + + ++ S E +K V + V LI L+RPKALNA+ + ++ ++++
Sbjct: 28 LSVSHRRKFSVMASGSDEFVKGNVYPN--GVALITLDRPKALNAMNLEMDLKYKSLLDEW 85
Query: 336 DADSNIAAIIITGN-EKAFAAGADIK----EMQNNTYSSNTKQGFLREWE---DISNCGK 491
+ D + +++ G+ +AF AG DIK E+ + +S ++ F E+ I+ K
Sbjct: 86 EYDPGVKCVVVEGSTSRAFCAGMDIKGVVAEILMDKNTSLVQKVFTAEYSLICKIAGYRK 145
Query: 492 PIIAAVNG 515
P I+ ++G
Sbjct: 146 PYISLMDG 153
>At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 387
Score = 44.4 bits (100), Expect = 5e-05
Identities = 23/74 (31%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = +3
Query: 225 VVGSKK-NVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGNEKAFAAGA 401
V+G +K +V L LNRP+ LN + + +L + + ++ D I+I G +AF+AG
Sbjct: 13 VIGEEKGSVRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLILIKGTGRAFSAGG 72
Query: 402 DIKEMQNNTYSSNT 443
D+K + S ++
Sbjct: 73 DLKVFYHGQESKDS 86
>At4g13360.1 68417.m02089 enoyl-CoA hydratase/isomerase family
protein similar to CoA-thioester hydrolase CHY1
(beta-hydroxyisobutyryl-CoA hydrolase) [Arabidopsis
thaliana] GI:8572760; contains Pfam profile PF00378:
enoyl-CoA hydratase/isomerase family protein
Length = 381
Score = 42.7 bits (96), Expect = 1e-04
Identities = 29/98 (29%), Positives = 55/98 (56%), Gaps = 8/98 (8%)
Frame = +3
Query: 246 VGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGN-EKAFAAGADIK---- 410
V LI L+R KALNA+ + ++ ++++++D + +I+ G+ +AF AG DIK
Sbjct: 19 VALITLDRTKALNAMNLDMDIKYKSFLDEWESDPRVKCVIVEGSTSRAFCAGMDIKGVAA 78
Query: 411 EMQNNTYSSNTKQGFLREWE---DISNCGKPIIAAVNG 515
E+Q + + ++ F E+ I+ KP I+ ++G
Sbjct: 79 EIQKDKNTPLVQKVFTAEYTLICAIAAYKKPYISLMDG 116
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 42.7 bits (96), Expect = 1e-04
Identities = 20/61 (32%), Positives = 35/61 (57%)
Frame = +3
Query: 225 VVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADSNIAAIIITGNEKAFAAGAD 404
+V K +V ++ NRPK LNAL + L + ++ D ++ +++ G +AF+AG D
Sbjct: 52 LVEEKSSVRILTFNRPKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAGGD 111
Query: 405 I 407
I
Sbjct: 112 I 112
>At5g56110.1 68418.m07000 myb family transcription factor contains
PFAM profile: Myb DNA binding domain PF00249
Length = 320
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +1
Query: 121 CWERMY*TSAKWYPQQAKHL*SFIAQHHTRTSRL 222
C E+ +W P++ L S+IAQH TR RL
Sbjct: 6 CCEKENVKRGQWTPEEDNKLASYIAQHGTRNWRL 39
>At4g15820.1 68417.m02407 wound-responsive protein-related contains
weak similarity to KED [Nicotiana tabacum]
gi|8096269|dbj|BAA95789
Length = 460
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -1
Query: 449 LFCVAAVCIVLHLLNISTSRKSLLVTSDDDGSNVAV 342
+F VC VL L + + S K+ V+SD +G+N+ +
Sbjct: 92 IFAFQTVCAVLFLGDSTKSEKTPEVSSDSEGNNLVL 127
>At5g05970.1 68418.m00661 transducin family protein / WD-40 repeat
family protein contains similarity to regulatory protein
Nedd1; contains Pfam PF00400: WD domain, G-beta repeat
(6 copies, 2 weak)|19804256|gb|AV785466.1|AV785466
Length = 781
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -1
Query: 422 VLHLLNISTSRKSLLVTSDDDGS 354
VL LL+ S S + LLVT+ DDG+
Sbjct: 180 VLRLLDYSRSSRHLLVTAGDDGT 202
>At2g34750.1 68415.m04267 RNA polymerase I specific transcription
initiation factor RRN3 family protein contains Pfam
PF05327: RNA polymerase I specific transcription
initiation factor RRN3; similar to RRN3 (GI:7670100)
[Homo sapiens] similar to RNA polymerase I specific
transcription initiation factor RRN3 (Swiss-Prot:P36070)
[Saccharomyces cerevisiae]
Length = 613
Score = 27.5 bits (58), Expect = 5.8
Identities = 19/68 (27%), Positives = 29/68 (42%), Gaps = 2/68 (2%)
Frame = +3
Query: 273 KALNALCKPLFV--ELGKAVNDFDADSNIAAIIITGNEKAFAAGADIKEMQNNTYSSNTK 446
K+ N+ P F+ + KA D D D N A +I+ G+E + + N S K
Sbjct: 526 KSSNSFISPNFIYWSMVKATYDEDDDDNDAEVIVNGDEDSDEDDEADLDYALNKMSITPK 585
Query: 447 QGFLREWE 470
F + E
Sbjct: 586 HSFKNKME 593
>At4g35180.1 68417.m05001 amino acid transporter family protein
similar to amino acid permease 1 GI:976402 from
[Nicotiana sylvestris]; contains Pfam profile PF01490:
Transmembrane amino acid transporter protein
Length = 456
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/35 (31%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -2
Query: 514 PLTAAMMG-FPQLEMSSHSRRKPCFVLLLYVLFCI 413
P T +G + +L HS+R CF+ L ++ C+
Sbjct: 297 PATGGPVGNYLKLYTQEHSKRAACFIHLTFIFSCL 331
>At3g28360.1 68416.m03544 ABC transporter family protein similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1158
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = +3
Query: 252 LIQLNRPKALNALCKPLFVELGKAVNDFDADSN---IAAIIITGNEK 383
L+ +NRP+ +ALC L LG AV A S+ I+ +T +E+
Sbjct: 577 LMAMNRPEWKHALCGCLSASLGGAVQPIYAYSSGLMISVFFLTNHEQ 623
>At2g39960.1 68415.m04910 microsomal signal peptidase 25 kDa
subunit, putative (SPC25) identical to Probable
microsomal signal peptidase 25 kDa subunit (EC 3.4.-.-)
(SPase 25 kDa subunit) (SPC25) (Swiss-Prot:P58684)
[Arabidopsis thaliana]; contains non-consensus AT-AC
splice sites; contains 1 transmembrane domain;
Length = 192
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = -1
Query: 443 CVAAVCI---VLHLLNISTSRKSLLVTSDDDGSNVAVGVKIIDCLPEFNKQW 297
C+A + VL L+ + + ++L T +GS + G+ + LP F+ Q+
Sbjct: 82 CIALYVVLNAVLQLILYTKEKNAILFTYPPEGSFTSTGLVVSSKLPRFSDQY 133
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,869,467
Number of Sequences: 28952
Number of extensions: 235346
Number of successful extensions: 654
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 636
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 653
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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