Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS314F11f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g71790.1 68414.m08297 F-actin capping protein beta subunit fa... 167 4e-42
At3g56100.1 68416.m06235 leucine-rich repeat transmembrane prote... 30 0.82
At3g23590.1 68416.m02967 expressed protein 29 2.5
At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH) domain... 28 3.3
At4g33520.3 68417.m04762 metal-transporting P-type ATPase, putat... 28 4.4
At4g33520.2 68417.m04761 metal-transporting P-type ATPase, putat... 28 4.4
At5g44440.1 68418.m05446 FAD-binding domain-containing protein s... 27 5.8
At5g42765.1 68418.m05208 expressed protein 27 5.8
At3g07640.1 68416.m00915 expressed protein 27 5.8
At5g48310.1 68418.m05968 expressed protein 27 7.7
At1g09020.1 68414.m01006 protein kinase, putative similar to pro... 27 7.7
>At1g71790.1 68414.m08297 F-actin capping protein beta subunit
family protein contains Pfam profile: PF01115 F-actin
capping protein, beta subunit
Length = 256
Score = 167 bits (406), Expect = 4e-42
Identities = 76/137 (55%), Positives = 98/137 (71%), Gaps = 1/137 (0%)
Frame = +3
Query: 114 MDCALDLMRRLPPQQIEKNLTDLIDLVPSMCDDLLSSVDQPLKIAXDRSNGKDYLLCDYN 293
M+ AL L+RR+PP+Q E L+ L+ L+P DLLS VD PL++ D +GKD++LC+YN
Sbjct: 1 MEAALGLLRRMPPKQSETALSALLSLIPQHSSDLLSQVDLPLQVLRDIESGKDFILCEYN 60
Query: 294 RDGDSYRSPWSNTYDPPLDDGSMPSERLRKLEIDANLAFDQYREMYFEGGVSSVYLWDMD 473
RD DSYRSPWSN Y PPL+D PS LRKLE++AN F YR+ Y+EGG+SSVY+W+ D
Sbjct: 61 RDADSYRSPWSNKYLPPLEDALYPSSELRKLEVEANDIFAIYRDQYYEGGISSVYMWEDD 120
Query: 474 H-GFAGVILIKKAGDGS 521
+ GF LIKK G S
Sbjct: 121 NEGFVACFLIKKDGSKS 137
>At3g56100.1 68416.m06235 leucine-rich repeat transmembrane protein
kinase, putative hypothetical proteins - Arabidopsis
thaliana
Length = 719
Score = 30.3 bits (65), Expect = 0.82
Identities = 27/115 (23%), Positives = 44/115 (38%)
Frame = +3
Query: 126 LDLMRRLPPQQIEKNLTDLIDLVPSMCDDLLSSVDQPLKIAXDRSNGKDYLLCDYNRDGD 305
LDL L + I NL D L+ +S+ + ++ RS+ +L D+N
Sbjct: 178 LDLSNNLLSEIIPPNLADSSKLLRLNLS--FNSLSGQIPVSLSRSSSLQFLALDHNNLSG 235
Query: 306 SYRSPWSNTYDPPLDDGSMPSERLRKLEIDANLAFDQYREMYFEGGVSSVYLWDM 470
W + L +LRK++I N E G +SS+ D+
Sbjct: 236 PILDTWGSKIRGTLPSELSKLTKLRKMDISGNSVSGHIPETL--GNISSLIHLDL 288
>At3g23590.1 68416.m02967 expressed protein
Length = 1309
Score = 28.7 bits (61), Expect = 2.5
Identities = 21/70 (30%), Positives = 34/70 (48%)
Frame = -3
Query: 321 KETYRSHRRDCNRIINSLSRCYDXEQSLEADLLMIIGRHTYSERGLSNLLNSFRSAEGAA 142
K YR H+R ++ L+ Q++EA L M G+ S+ L+ + + S+ GAA
Sbjct: 767 KVWYRQHQRCIAATLSGLTHGSPVHQTVEALLNMTFGKVRGSQT-LNPVNSGTSSSSGAA 825
Query: 141 SASSPVHNPF 112
S S + F
Sbjct: 826 SEDSNIRPEF 835
>At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related Aux22d, Vigna radiata, PID:D1021691;
contains Pfam PF01417: ENTH domain. ENTH (Epsin
N-terminal homology) domain; similar to clathrin
assembly protein AP180 (GI:6492344) [Xenopus laevis]
Length = 635
Score = 28.3 bits (60), Expect = 3.3
Identities = 17/38 (44%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = +3
Query: 255 RSNGKDYLLCDYNRDGDSYRSPWSNTYDPPLDDG-SMP 365
+SNG D YNR D +RSP TYD +G MP
Sbjct: 169 QSNGDD----GYNRSRDDFRSPPPRTYDYETGNGFGMP 202
>At4g33520.3 68417.m04762 metal-transporting P-type ATPase, putative
(PAA1) nearly identical to gi:2668492; contains Pfam
heavy-metal-associated domain PF00403
Length = 949
Score = 27.9 bits (59), Expect = 4.4
Identities = 20/70 (28%), Positives = 29/70 (41%), Gaps = 4/70 (5%)
Frame = -3
Query: 315 TYRSHRRDCNRIINSLSRCYDXEQSLEADLLMII----GRHTYSERGLSNLLNSFRSAEG 148
T HR + + R + QS EA + ++ GR TY LS +F + G
Sbjct: 466 TVEVHRSGGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFWNLFG 525
Query: 147 AASASSPVHN 118
A S +HN
Sbjct: 526 AHVLPSALHN 535
>At4g33520.2 68417.m04761 metal-transporting P-type ATPase, putative
(PAA1) nearly identical to gi:2668492; contains Pfam
heavy-metal-associated domain PF00403
Length = 949
Score = 27.9 bits (59), Expect = 4.4
Identities = 20/70 (28%), Positives = 29/70 (41%), Gaps = 4/70 (5%)
Frame = -3
Query: 315 TYRSHRRDCNRIINSLSRCYDXEQSLEADLLMII----GRHTYSERGLSNLLNSFRSAEG 148
T HR + + R + QS EA + ++ GR TY LS +F + G
Sbjct: 466 TVEVHRSGGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFWNLFG 525
Query: 147 AASASSPVHN 118
A S +HN
Sbjct: 526 AHVLPSALHN 535
>At5g44440.1 68418.m05446 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 533
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -3
Query: 444 RHLQSTSLDIDQRRGW 397
R+L+S SLD+D R GW
Sbjct: 131 RNLRSISLDVDNRTGW 146
>At5g42765.1 68418.m05208 expressed protein
Length = 229
Score = 27.5 bits (58), Expect = 5.8
Identities = 20/80 (25%), Positives = 37/80 (46%)
Frame = -3
Query: 393 LFLVFSSVQRAWSHHLMVGRKYSTKETYRSHRRDCNRIINSLSRCYDXEQSLEADLLMII 214
L L S A++ L + KE + ++ +R + L+ Q+L++ L +
Sbjct: 52 LLLTLSPTNPAFAFSLGIS---GPKEWLKDQKKKSSRFL--LAPIDATRQALQSAYLSLT 106
Query: 213 GRHTYSERGLSNLLNSFRSA 154
Y+E+ L NL N F+S+
Sbjct: 107 SESDYTEKDLENLQNLFKSS 126
>At3g07640.1 68416.m00915 expressed protein
Length = 241
Score = 27.5 bits (58), Expect = 5.8
Identities = 20/57 (35%), Positives = 26/57 (45%)
Frame = +3
Query: 297 DGDSYRSPWSNTYDPPLDDGSMPSERLRKLEIDANLAFDQYREMYFEGGVSSVYLWD 467
D D + + S+T +P PS LRKLE L D+Y E G S V+ D
Sbjct: 13 DSDGFAATISDTLNPS------PSSPLRKLEEQIQLPLDKYGVEGVETGGSIVHFVD 63
>At5g48310.1 68418.m05968 expressed protein
Length = 1156
Score = 27.1 bits (57), Expect = 7.7
Identities = 21/89 (23%), Positives = 38/89 (42%), Gaps = 1/89 (1%)
Frame = +3
Query: 150 PQQIEKNLTDLIDLVPSMCDDLLSSVDQPLKIAXDRSNGKDYLLCDYNRDGDSYRSPWSN 329
P + NL+ I + P++ DD S+ + ++ D G + L DY+ + +
Sbjct: 26 PLKRNSNLSSPILVTPTLNDDE-SNDNMSIESVSDTGEGNELLFSDYDVEDEEEEEVIGR 84
Query: 330 TYDPPLDDGSMPSERL-RKLEIDANLAFD 413
YD G + +L R + D NL +
Sbjct: 85 RYDEEEVFGDKSNSKLNRGMLKDKNLRIE 113
>At1g09020.1 68414.m01006 protein kinase, putative similar to
protein kinase AKINbetagamma-2 [Zea mays] GI:11139548,
AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam
profile PF00571: CBS domain
Length = 487
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/38 (34%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
Frame = +3
Query: 384 LEIDANLAFDQYREMYFEGGVSSVYLWDMDHG-FAGVI 494
+ +D NL Q + +E G+ LWD G F GV+
Sbjct: 173 IALDVNLPVKQAFHILYEQGIPLAPLWDFGKGQFVGVL 210
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,334,635
Number of Sequences: 28952
Number of extensions: 203558
Number of successful extensions: 592
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 592
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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