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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS314E12f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g04845.1 68415.m00499 GCN5-related N-acetyltransferase (GNAT)...   174   3e-44
At3g18600.1 68416.m02364 DEAD/DEAH box helicase, putative non-co...    33   0.088
At4g04635.1 68417.m00678 hypothetical protein                          29   1.4  
At2g34100.1 68415.m04175 expressed protein  similar to the Asp-r...    29   1.4  
At1g50930.1 68414.m05726 hypothetical protein                          28   3.3  
At2g03730.1 68415.m00333 ACT domain-containing protein (ACR5) co...    27   5.8  
At1g71390.1 68414.m08243 disease resistance family protein / LRR...    27   5.8  
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp...    27   5.8  
At1g01440.1 68414.m00059 extra-large G-protein-related weak simi...    27   5.8  
At3g59380.1 68416.m06622 farnesyltransferase alpha subunit, puta...    27   7.7  
At3g54530.1 68416.m06034 hypothetical protein                          27   7.7  
At3g22930.1 68416.m02889 calmodulin, putative strong similarity ...    27   7.7  
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina...    27   7.7  
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina...    27   7.7  

>At2g04845.1 68415.m00499 GCN5-related N-acetyltransferase (GNAT)
           family protein contains Pfam profile PF00583:
           acetyltransferase, GNAT family
          Length = 218

 Score =  174 bits (423), Expect = 3e-44
 Identities = 82/176 (46%), Positives = 120/176 (68%), Gaps = 4/176 (2%)
 Frame = +1

Query: 1   KLNSHIKIVGANIILVPYKKHHVPVYHEWMKSEELQKLTASEPLNLEQEYEMQKTWHDDE 180
           ++ + + + G  ++LVPY   HVP YH+WM+   L + T SEPL+LEQEYEMQ +W  D 
Sbjct: 4   RMATKVSLEGKRVVLVPYMAEHVPKYHQWMQDSALLEATGSEPLSLEQEYEMQLSWTQDP 63

Query: 181 DKCTFIILDKD-IFNNTAFDE--IDAMIGDTNIFITD-KELAIGEIEIMIAEPSARGKKR 348
           +K TFI+LDKD +  + A  +  ++AM GD NI++ D  +  + E+EIMIAEP +RGK  
Sbjct: 64  NKRTFIVLDKDFVKGDLAHGQPHVEAMTGDVNIYMNDVDDPKVAEVEIMIAEPRSRGKGL 123

Query: 349 GWEAVKLMLLYGIKYIKIQTFEAKISLQNVISIKMFKKLGFQEKSVSAVFQEVTLD 516
           G E+V +M+ YG+K ++I  F AKI   N  S+ +F+KLGF+E S S +F+EVTL+
Sbjct: 124 GKESVLIMMAYGVKNLEIHKFTAKIGESNTASLSLFRKLGFEESSYSGIFKEVTLE 179


>At3g18600.1 68416.m02364 DEAD/DEAH box helicase, putative
           non-consensus acceptor splice site AT at exon 2; similar
           to DEAD box helicase protein GB:NP_006764 from [Homo
           sapiens], contains Pfam profile: PF00270  DEAD/DEAH box
           helicase
          Length = 568

 Score = 33.5 bits (73), Expect = 0.088
 Identities = 33/127 (25%), Positives = 58/127 (45%), Gaps = 9/127 (7%)
 Frame = +1

Query: 136 LEQEYEMQKTWHDDEDKCTFIILDKDIFNNTAFDEIDAMIGDTNIFITD------KELAI 297
           +E+E E  +   D ED+   +I+ K I  N  FD +D +   T+I I +       ++  
Sbjct: 59  IEEEEEKVEAMEDGEDEKNIVIVGKGIMTNVTFDSLD-LSEQTSIAIKEMGFQYMTQIQA 117

Query: 298 GEIEIMIAEPSARGKKRGWEAVKLMLLYGIKYIKIQTFEAKISLQN---VISIKMFKKLG 468
           G I+ ++      G  R      L  L  I  +++  F+ + S +N   VI I   ++L 
Sbjct: 118 GSIQPLLEGKDVLGAARTGSGKTLAFL--IPAVEL-LFKERFSPRNGTGVIVICPTRELA 174

Query: 469 FQEKSVS 489
            Q K+V+
Sbjct: 175 IQTKNVA 181


>At4g04635.1 68417.m00678 hypothetical protein
          Length = 284

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
 Frame = +1

Query: 16  IKIVGANIILVPY--KKHHVPVYHEWMKSEELQKLTASEPLNLEQEYEMQKTWHDDE 180
           + IVG +IILV    + HH+  YH   + EE ++    E    E+E E ++   ++E
Sbjct: 195 VHIVGCDIILVSLVEEDHHIEEYHVDDEEEEEEEEGEEEEGEGEEEEEEEEEGEEEE 251


>At2g34100.1 68415.m04175 expressed protein  similar to the Asp-rich
           region of GP|1633572|U52064
          Length = 345

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 17/63 (26%), Positives = 35/63 (55%), Gaps = 6/63 (9%)
 Frame = +1

Query: 91  KSEELQKLTASEPLNLEQEY---EMQKTWHDDEDKCT--FIILDKD-IFNNTAFDEIDAM 252
           + +E  +  + E + + +E    E++K  HDDED  +  F+ + ++ I  NT F E++ +
Sbjct: 274 EDDEAGEYHSEEAMEMSREQRMSELRKVLHDDEDDVSQGFVNVRRETIAKNTEFGEVETL 333

Query: 253 IGD 261
           + D
Sbjct: 334 LPD 336


>At1g50930.1 68414.m05726 hypothetical protein
          Length = 196

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 38/127 (29%), Positives = 55/127 (43%), Gaps = 1/127 (0%)
 Frame = +1

Query: 1   KLNSHIKIVGANIILVPYKKHHVPVYHEWMKSEELQKLTASEPLNLEQEYEMQKTWHDDE 180
           K+N+ ++    NI   P KK  +   H+  + EELQK         E+E + + T     
Sbjct: 75  KINNVVRRKANNINTNP-KKRRIIHQHKEEEEEELQK-------GEEEEEDEEDTASSPS 126

Query: 181 DKCT-FIILDKDIFNNTAFDEIDAMIGDTNIFITDKELAIGEIEIMIAEPSARGKKRGWE 357
           +K   F +LD    N      +D +  +    IT+    I EI  M  E SA  KKRG  
Sbjct: 127 NKTKIFSVLDHANDNTRYGKTMDNVTSEEIGCITETGSKIKEI--MNEEFSAELKKRGLC 184

Query: 358 AVKLMLL 378
            V L +L
Sbjct: 185 VVPLSML 191


>At2g03730.1 68415.m00333 ACT domain-containing protein (ACR5)
           contains Pfam ACT domain PF01842
          Length = 456

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 139 EQEYEMQKTWHDDEDKCTFIILDKDIFNNTAFDEIDAMI 255
           +++Y+  +   DDEDKC  +I D D+ N    D    MI
Sbjct: 236 DRDYDEWENNVDDEDKCGRVIPDVDVSNLHDLDYSIVMI 274


>At1g71390.1 68414.m08243 disease resistance family protein / LRR
           family protein contains leucine rich-repeat domains
           Pfam:PF00560, INTERPRO:IPR001611; similar to Hcr2-5B
           [Lycopersicon esculentum] gi|3894391|gb|AAC78595
          Length = 784

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 3/42 (7%)
 Frame = +1

Query: 196 IILDKDIFNNTAFDEIDAMIGDTN---IFITDKELAIGEIEI 312
           ++LD D++NN+   E+ A IG+ N   +   D+    G I I
Sbjct: 175 LLLDLDLWNNSLVGEVPASIGNLNELRVMSLDRNSLSGSIPI 216


>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
           domain-containing protein / ubiquitin family protein
           similar to SP|Q15459 Splicing factor 3 subunit 1
           (Spliceosome associated protein 114) {Homo sapiens};
           contains Pfam profiles PF00240: Ubiquitin family,
           PF01805: Surp module
          Length = 785

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 27/119 (22%), Positives = 51/119 (42%)
 Frame = +1

Query: 82  EWMKSEELQKLTASEPLNLEQEYEMQKTWHDDEDKCTFIILDKDIFNNTAFDEIDAMIGD 261
           EW +S+E QK    +   LE+       WHD      F++++   F +   +E+   +  
Sbjct: 275 EWDRSQEQQKKKEEDEKELERVQMAMIDWHD------FVVVESIDFADEEDEELPPPMTL 328

Query: 262 TNIFITDKELAIGEIEIMIAEPSARGKKRGWEAVKLMLLYGIKYIKIQTFEAKISLQNV 438
             +    K  A+ E EI+  EP   GK+   E  +  +    + ++    E  + ++NV
Sbjct: 329 DEVIRRSKASAMEEDEIV--EP---GKEVEMEMDEEEVKLVAEGMRAANLEENVKIENV 382


>At1g01440.1 68414.m00059 extra-large G-protein-related weak
           similarity to extra-large G-protein  (AtXLG1)
           (GI:3201682) [Arabidopsis thaliana]
          Length = 664

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 10/33 (30%), Positives = 21/33 (63%)
 Frame = +1

Query: 256 GDTNIFITDKELAIGEIEIMIAEPSARGKKRGW 354
           G+T++F+  +EL   ++E++ +    RGK R +
Sbjct: 588 GNTSVFVNGRELHERDLELLSSRGLPRGKNRSY 620


>At3g59380.1 68416.m06622 farnesyltransferase alpha subunit,
           putative / FTA, putative / protein farnesyltransferase,
           putative similar to farnesyltransferase alpha subunit
           [GI:2246442][Pisum sativum]
          Length = 326

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +1

Query: 166 WHDDEDKCTFIILDKDIFNNTAFDE 240
           W D+ D C   +L+ D+FNN+A+++
Sbjct: 161 WEDELDYC-HELLEADVFNNSAWNQ 184


>At3g54530.1 68416.m06034 hypothetical protein 
          Length = 273

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 8/19 (42%), Positives = 14/19 (73%)
 Frame = +3

Query: 453 VQKVRISGEKCQCSISRSD 509
           ++K+R+  EKC+C + R D
Sbjct: 216 IEKIRVLREKCKCIVERRD 234


>At3g22930.1 68416.m02889 calmodulin, putative strong similarity to
           calmodulin 8 GI:5825600 from [Arabidopsis thaliana];
           contains INTERPRO:IPR002048 calcium-binding EF-hand
           domain
          Length = 173

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 25/88 (28%), Positives = 45/88 (51%)
 Frame = +1

Query: 121 SEPLNLEQEYEMQKTWHDDEDKCTFIILDKDIFNNTAFDEIDAMIGDTNIFITDKELAIG 300
           SE LNL    ++Q+T  D+E K  F + DKD     +  E+  ++ +    +TD+E+   
Sbjct: 90  SEFLNLMAN-QLQETDADEELKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEV--- 145

Query: 301 EIEIMIAEPSARGKKRGWEAVKLMLLYG 384
           +  I  A+    G+    E V++M++ G
Sbjct: 146 DQMIKEADLDGDGQVNYDEFVRMMMING 173


>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
           region is similar to C-term region of kinesin motor
           protein GB:AAB51397 (Mus musculus); contains Pfam
           profile: PF00225 Kinesin motor domain
          Length = 1065

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 18/76 (23%), Positives = 36/76 (47%)
 Frame = +1

Query: 121 SEPLNLEQEYEMQKTWHDDEDKCTFIILDKDIFNNTAFDEIDAMIGDTNIFITDKELAIG 300
           +E   L+QE ++ K  H  E++C    L+ +       DE++  + D  + + D    + 
Sbjct: 326 AEIRQLKQELKLVKETH--ENQC----LELEAKAQKTRDELEKKLKDAELHVVDSSRKVK 379

Query: 301 EIEIMIAEPSARGKKR 348
           E+E +    S R +K+
Sbjct: 380 ELEKLCQSKSQRWEKK 395


>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
           region is similar to C-term region of kinesin motor
           protein GB:AAB51397 (Mus musculus); contains Pfam
           profile: PF00225 Kinesin motor domain
          Length = 1064

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 18/76 (23%), Positives = 36/76 (47%)
 Frame = +1

Query: 121 SEPLNLEQEYEMQKTWHDDEDKCTFIILDKDIFNNTAFDEIDAMIGDTNIFITDKELAIG 300
           +E   L+QE ++ K  H  E++C    L+ +       DE++  + D  + + D    + 
Sbjct: 326 AEIRQLKQELKLVKETH--ENQC----LELEAKAQKTRDELEKKLKDAELHVVDSSRKVK 379

Query: 301 EIEIMIAEPSARGKKR 348
           E+E +    S R +K+
Sbjct: 380 ELEKLCQSKSQRWEKK 395


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,660,347
Number of Sequences: 28952
Number of extensions: 173019
Number of successful extensions: 551
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 549
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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