BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS314D08f (521 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_5211| Best HMM Match : 7tm_1 (HMM E-Value=0.015) 29 2.3 SB_48109| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.4 SB_58032| Best HMM Match : Rho_N (HMM E-Value=0.0007) 27 7.1 >SB_5211| Best HMM Match : 7tm_1 (HMM E-Value=0.015) Length = 728 Score = 29.1 bits (62), Expect = 2.3 Identities = 12/33 (36%), Positives = 23/33 (69%) Frame = -1 Query: 491 AVKFTCIRLLFTEVFCLQLVNLKRYFALLL*IK 393 A++FT L ++F + L++L++YF+LL +K Sbjct: 113 ALRFTTCTLSSIKIFSILLISLEKYFSLLYPLK 145 >SB_48109| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 399 Score = 27.9 bits (59), Expect = 5.4 Identities = 13/39 (33%), Positives = 20/39 (51%) Frame = -3 Query: 204 EKSHAFLIKTHPVM*FSEPREIKSLPQLFSITATHLSKW 88 EKS + L K + + PRE L ++ +T T +S W Sbjct: 188 EKSRSILNKAYVDSPYPTPREKHELAKMTDLTVTQVSNW 226 >SB_58032| Best HMM Match : Rho_N (HMM E-Value=0.0007) Length = 243 Score = 27.5 bits (58), Expect = 7.1 Identities = 13/40 (32%), Positives = 22/40 (55%) Frame = +2 Query: 338 FHKS*NDDLLCLENFCNDLLFTKVTQNIFLNLLIVDKTLQ 457 F K+ +D +CL N NDL + K + ++ + L D T + Sbjct: 56 FLKTDDDYFVCLNNLANDLQYRKNEKYLYWDWLGCDPTFE 95 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,093,521 Number of Sequences: 59808 Number of extensions: 257724 Number of successful extensions: 415 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 356 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 415 length of database: 16,821,457 effective HSP length: 77 effective length of database: 12,216,241 effective search space used: 1172759136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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