Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS314D01f
(452 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g52870.2 68414.m05978 peroxisomal membrane protein-related co... 42 1e-04
At5g19750.1 68418.m02348 peroxisomal membrane 22 kDa family prot... 40 8e-04
At4g03410.2 68417.m00465 peroxisomal membrane protein-related co... 40 8e-04
At4g03410.1 68417.m00464 peroxisomal membrane protein-related co... 40 8e-04
At4g14310.1 68417.m02204 peroxisomal membrane protein-related co... 39 0.001
At4g04470.1 68417.m00649 peroxisomal membrane protein 22 kDa (PM... 36 0.010
At2g14860.1 68415.m01689 peroxisomal membrane protein 22 kDa, pu... 36 0.017
At2g25080.1 68415.m03001 phospholipid hydroperoxide glutathione ... 31 0.48
At5g11220.1 68418.m01311 expressed protein 29 1.5
At1g52870.1 68414.m05977 peroxisomal membrane protein-related co... 29 1.5
At1g69070.1 68414.m07903 expressed protein 28 3.4
At4g01020.1 68417.m00137 helicase domain-containing protein / IB... 27 4.5
At5g10370.1 68418.m01203 helicase domain-containing protein / IB... 27 5.9
At4g24000.1 68417.m03449 cellulose synthase family protein simil... 27 5.9
At5g43140.1 68418.m05266 peroxisomal membrane 22 kDa family prot... 27 7.8
At1g73720.1 68414.m08536 transducin family protein / WD-40 repea... 27 7.8
>At1g52870.2 68414.m05978 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:Q07066 22 kDa
peroxisomal membrane protein [Rattus norvegicus]
Length = 366
Score = 42.3 bits (95), Expect = 1e-04
Identities = 25/101 (24%), Positives = 50/101 (49%), Gaps = 2/101 (1%)
Frame = +1
Query: 148 TVPHYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQA--FSLYSLARFEGKTHRAAL 321
++ H++Y+ E LFP + + + K+ ++ +++ + + F++ RFE +
Sbjct: 229 SLSHFYYQFCEELFPFQDW-WVVPVKVAFDQTVWSAIWNSIYFTVLGFLRFESPI--SIF 285
Query: 322 KQLFALYLPVLEANWKWLTLFQVINLAFIPPMLRVCHEHCV 444
K+L A +LP+L A WK +I +P R+ CV
Sbjct: 286 KELKATFLPMLTAGWKLWPFAHLITYGLVPVEQRLLWVDCV 326
>At5g19750.1 68418.m02348 peroxisomal membrane 22 kDa family protein
similar to SP|P42925 22 kDa peroxisomal membrane protein
{Mus musculus}; contains Pfam profile PF04117: Mpv17 /
PMP22 family
Length = 288
Score = 39.9 bits (89), Expect = 8e-04
Identities = 24/96 (25%), Positives = 46/96 (47%)
Frame = +1
Query: 157 HYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQAFSLYSLARFEGKTHRAALKQLFA 336
H++Y + ++ S + + LLL++ +FAP+ L ++ EGK + +L
Sbjct: 176 HFWYLYLSKVVTASGLSGAVIR-LLLDQFVFAPIFVGVFLSAVVTLEGKPSN-VIPKLQQ 233
Query: 337 LYLPVLEANWKWLTLFQVINLAFIPPMLRVCHEHCV 444
+ + ANW+ FQ +N F+P +V + V
Sbjct: 234 EWTGAMIANWQLWIPFQFLNFRFVPQNYQVLASNVV 269
>At4g03410.2 68417.m00465 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:P42925 22 kDa
peroxisomal membrane protein [Mus musculus]
Length = 361
Score = 39.9 bits (89), Expect = 8e-04
Identities = 23/101 (22%), Positives = 49/101 (48%), Gaps = 2/101 (1%)
Frame = +1
Query: 148 TVPHYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQA--FSLYSLARFEGKTHRAAL 321
++ HY+Y+ E LFP + + + K+ ++ +++ + + F++ L RF+
Sbjct: 187 SLSHYYYQFCEALFPFQEW-WVVPAKVAFDQTVWSAIWNSIYFTVLGLLRFQSPAD--IF 243
Query: 322 KQLFALYLPVLEANWKWLTLFQVINLAFIPPMLRVCHEHCV 444
++ +LP+L A WK L ++ IP R+ C+
Sbjct: 244 SEIKTTFLPMLTAGWKLWPLAHLVTYGVIPVDQRLLWVDCI 284
>At4g03410.1 68417.m00464 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:P42925 22 kDa
peroxisomal membrane protein [Mus musculus]
Length = 317
Score = 39.9 bits (89), Expect = 8e-04
Identities = 23/101 (22%), Positives = 49/101 (48%), Gaps = 2/101 (1%)
Frame = +1
Query: 148 TVPHYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQA--FSLYSLARFEGKTHRAAL 321
++ HY+Y+ E LFP + + + K+ ++ +++ + + F++ L RF+
Sbjct: 187 SLSHYYYQFCEALFPFQEW-WVVPAKVAFDQTVWSAIWNSIYFTVLGLLRFQSPAD--IF 243
Query: 322 KQLFALYLPVLEANWKWLTLFQVINLAFIPPMLRVCHEHCV 444
++ +LP+L A WK L ++ IP R+ C+
Sbjct: 244 SEIKTTFLPMLTAGWKLWPLAHLVTYGVIPVDQRLLWVDCI 284
>At4g14310.1 68417.m02204 peroxisomal membrane protein-related
contains weak similarity to Peroxisomal membrane protein
2 (22 kDa peroxisomal membrane protein)
(Swiss-Prot:P42925) [Mus musculus]
Length = 1087
Score = 39.1 bits (87), Expect = 0.001
Identities = 23/91 (25%), Positives = 44/91 (48%), Gaps = 1/91 (1%)
Frame = +1
Query: 157 HYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQ-AFSLYSLARFEGKTHRAALKQLF 333
H+F++ ++ +F + + +AKK+LLE+L +P F Y EG+ + +L
Sbjct: 971 HFFHKLMDTIFKGKKGNSTVAKKVLLEQLTSSPWNNFLFMSYYGLVVEGRPWKLVKHKLG 1030
Query: 334 ALYLPVLEANWKWLTLFQVINLAFIPPMLRV 426
Y + WK+ + +N ++P RV
Sbjct: 1031 KDYPTIQLTAWKFWPIVGWVNYQYVPLQFRV 1061
>At4g04470.1 68417.m00649 peroxisomal membrane protein 22 kDa
(PMP22) identical to peroxisomal membrane protein
[Arabidopsis thaliana] gi|3980254|emb|CAA06834
Length = 190
Score = 36.3 bits (80), Expect = 0.010
Identities = 28/130 (21%), Positives = 55/130 (42%), Gaps = 2/130 (1%)
Frame = +1
Query: 43 VVGTAGSLASQIVAG-ESIRLDPIXXXXXXXXXXXXTVPHYFYETVERLFPEESASFPLA 219
V+ + SQ ++G + I+L + H+F+ +++ F + + +A
Sbjct: 32 VLSGVSDVVSQKLSGIQKIQLRRVLLKVIFAGGFLGPAGHFFHTYLDKFFKGKKDTQTVA 91
Query: 220 KKLLLERLIFAPLMQ-AFSLYSLARFEGKTHRAALKQLFALYLPVLEANWKWLTLFQVIN 396
KK++LE+L +PL F +Y E +++ Y V W + + IN
Sbjct: 92 KKVILEQLTLSPLNHLLFMIYYGVVIERTPWTLVRERIKKTYPTVQLTAWTFFPVVGWIN 151
Query: 397 LAFIPPMLRV 426
++P RV
Sbjct: 152 YKYVPLHFRV 161
>At2g14860.1 68415.m01689 peroxisomal membrane protein 22 kDa,
putative similar to 22 kDa peroxisomal membrane protein
{Mus musculus} SWISS-PROT:P42925, NCBI_gi:454833
Length = 252
Score = 35.5 bits (78), Expect = 0.017
Identities = 23/89 (25%), Positives = 38/89 (42%)
Frame = +1
Query: 157 HYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQAFSLYSLARFEGKTHRAALKQLFA 336
HY++ + RLFP++ KK+ + + I+ P+M A +G+ L +L
Sbjct: 136 HYWFNFMSRLFPKQDL-ITTFKKMAMGQTIYGPIMTVIFFSLNASLQGERGSVILARLKR 194
Query: 337 LYLPVLEANWKWLTLFQVINLAFIPPMLR 423
LP L + L I F P L+
Sbjct: 195 DLLPALFNGVMYWPLCDFITFRFFPVHLQ 223
>At2g25080.1 68415.m03001 phospholipid hydroperoxide glutathione
peroxidase, chloroplast / PHGPx (GPX1) identical to
SP|P52032 Phospholipid hydroperoxide glutathione
peroxidase, chloroplast precursor (EC 1.11.1.9) (PHGPx)
{Arabidopsis thaliana}; contains Glutathione peroxidases
signatures, Glutathione_Peroxid_1 [GKVMLIVNVASRCGLT],
Glutathione_Peroxid_2 [LAFPCNQF]; contains EST
GB:T43669, N38679, R30227, H37043, AA042773; identical
to cDNA chloroplast mRNA for glutathione peroxidase
GI:2274856
Length = 236
Score = 30.7 bits (66), Expect = 0.48
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +3
Query: 312 SRSEATFCIVLTSLRSQLEMAYIVPSHKFSIYTSNVESLS 431
S S TF V+ S R ++VPS KFS SN +LS
Sbjct: 8 SSSYGTFSTVVNSSRPNSSATFLVPSLKFSTGISNFANLS 47
>At5g11220.1 68418.m01311 expressed protein
Length = 265
Score = 29.1 bits (62), Expect = 1.5
Identities = 14/28 (50%), Positives = 17/28 (60%), Gaps = 1/28 (3%)
Frame = -1
Query: 284 KEYRENACINGAKIKRSNNNFF-ASGND 204
K Y EN C+ G +K N NF A+GND
Sbjct: 190 KNYGEN-CVEGVYMKEDNKNFLNANGND 216
>At1g52870.1 68414.m05977 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:Q07066 22 kDa
peroxisomal membrane protein [Rattus norvegicus]
Length = 306
Score = 29.1 bits (62), Expect = 1.5
Identities = 17/71 (23%), Positives = 39/71 (54%), Gaps = 2/71 (2%)
Frame = +1
Query: 148 TVPHYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQA--FSLYSLARFEGKTHRAAL 321
++ H++Y+ E LFP + + + K+ ++ +++ + + F++ RFE +
Sbjct: 229 SLSHFYYQFCEELFPFQD-WWVVPVKVAFDQTVWSAIWNSIYFTVLGFLRFESPI--SIF 285
Query: 322 KQLFALYLPVL 354
K+L A +LP+L
Sbjct: 286 KELKATFLPML 296
>At1g69070.1 68414.m07903 expressed protein
Length = 901
Score = 27.9 bits (59), Expect = 3.4
Identities = 18/72 (25%), Positives = 38/72 (52%)
Frame = -2
Query: 343 STMQKVASERLYEFFLQIEPKNIEKMLALTVQRSNVPIITFLQVGMMQILQETAFQLFHR 164
+ M+K++S + F+ PKN E++LAL SN +I L V ++I + +R
Sbjct: 450 TNMKKMSSTQRDIPFMIDPPKNFEELLALVEDCSNEDVI--LIVNRIRIAHSIKIKAENR 507
Query: 163 NSEELYRQIIIR 128
+++ ++++
Sbjct: 508 KKMQVFYGVLLQ 519
>At4g01020.1 68417.m00137 helicase domain-containing protein / IBR
domain-containing protein / zinc finger protein-related
similar to SP|Q14562 ATP-dependent helicase DDX8 (RNA
helicase HRH1) (DEAH-box protein 8) {Homo sapiens};
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger),
PF01485: IBR domain
Length = 1787
Score = 27.5 bits (58), Expect = 4.5
Identities = 19/68 (27%), Positives = 31/68 (45%)
Frame = +1
Query: 169 ETVERLFPEESASFPLAKKLLLERLIFAPLMQAFSLYSLARFEGKTHRAALKQLFALYLP 348
E +R+F SA P + ++ +F P + + +L +F+G+ H A K L L
Sbjct: 1262 ELHKRIFARMSAKNPEPNCVQVQ--VFEPKEDNYFMRALIKFDGRLHLEAAKALQELNGE 1319
Query: 349 VLEANWKW 372
VL W
Sbjct: 1320 VLPGCLPW 1327
>At5g10370.1 68418.m01203 helicase domain-containing protein / IBR
domain-containing protein / zinc finger protein-related
similar to RNA-dependent ATPase/helicase Cdc28p
[Schizosaccharomyces pombe] GI:1439562; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain, weak hit
to PF00097: Zinc finger, C3HC4 type (RING finger),
PF01485: IBR domain
Length = 1775
Score = 27.1 bits (57), Expect = 5.9
Identities = 19/68 (27%), Positives = 31/68 (45%)
Frame = +1
Query: 169 ETVERLFPEESASFPLAKKLLLERLIFAPLMQAFSLYSLARFEGKTHRAALKQLFALYLP 348
E +R+F SA P + ++ +F P + + +L +F+G+ H A K L L
Sbjct: 1265 ELHKRIFARMSAKNPEPNCVQVQ--VFEPKEDNYFMRALIKFDGRLHFEAAKALQELNGE 1322
Query: 349 VLEANWKW 372
VL W
Sbjct: 1323 VLPGCLPW 1330
>At4g24000.1 68417.m03449 cellulose synthase family protein similar
to cellulose synthase from Gossypium hirsutum
[gi:1706956], cellulose synthase-5 from Zea mays
[gi:9622882]
Length = 722
Score = 27.1 bits (57), Expect = 5.9
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +3
Query: 369 MAYIVPSHKFSIYTSNVESLS*TL 440
MAY PSHK S+Y S+ S TL
Sbjct: 123 MAYEYPSHKISVYVSDDGGSSLTL 146
>At5g43140.1 68418.m05266 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 254
Score = 26.6 bits (56), Expect = 7.8
Identities = 14/89 (15%), Positives = 38/89 (42%)
Frame = +1
Query: 157 HYFYETVERLFPEESASFPLAKKLLLERLIFAPLMQAFSLYSLARFEGKTHRAALKQLFA 336
H ++ + ++ P+ KK+++ +++F P+ A +G+ + +L
Sbjct: 141 HLWFSYLSKILPKRDV-LTTFKKIMMGQVLFGPVSNTVFYSYNAALQGENSEEIVARLKR 199
Query: 337 LYLPVLEANWKWLTLFQVINLAFIPPMLR 423
LP L+ + + + ++P L+
Sbjct: 200 DLLPTLKNGLMYWPVCDFVTFKYVPVHLQ 228
>At1g73720.1 68414.m08536 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
similar to Will die slowly protein
(SP:Q9V3J8)[Drosophila melanogaster]
Length = 511
Score = 26.6 bits (56), Expect = 7.8
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 193 QETAFQLFHRNSEELYRQIIIRKNQMRE*DQV*LILQPRSVM 68
Q + +L E+LY QI++ ++RE D IL+ VM
Sbjct: 62 QVSQLKLPRNKLEDLYEQIVLEMIELRELDTARAILRQTQVM 103
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,565,844
Number of Sequences: 28952
Number of extensions: 172239
Number of successful extensions: 494
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 481
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 489
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 742437000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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