Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS313C03f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g45030.1 68415.m05606 mitochondrial elongation factor, putati...   122   2e-28
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati...   122   2e-28
At1g62750.1 68414.m07082 elongation factor Tu family protein sim...    89   2e-18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim...    35   0.038
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p...    33   0.12 
At5g25230.1 68418.m02991 elongation factor Tu family protein tra...    29   2.5  
At1g06220.2 68414.m00656 elongation factor Tu family protein sim...    29   2.5  
At1g06220.1 68414.m00655 elongation factor Tu family protein sim...    29   2.5  
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-...    28   3.3  
At3g47660.1 68416.m05188 regulator of chromosome condensation (R...    27   5.8  
At3g26580.1 68416.m03318 expressed protein                             27   7.7  
At3g14590.1 68416.m01847 C2 domain-containing protein low simila...    27   7.7  

>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
            similar to SP|P25039 Elongation factor G 1, mitochondrial
            precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains
            Pfam profiles PF00009: Elongation factor Tu GTP binding
            domain, PF03764: Elongation factor G domain IV, PF00679:
            Elongation factor G C-terminus
          Length = 754

 Score =  122 bits (293), Expect = 2e-28
 Identities = 65/132 (49%), Positives = 80/132 (60%)
 Frame = -3

Query: 516  VSGVKFRLQDGAHHIVDSSELAFFLAAKGAVKDVFDVGAWQILEPVMFVEVTLPDEFHGT 337
            V  ++  L DGA H VDSSELAF +AA  A +  +      ILEPVM VE+ +P EF GT
Sbjct: 616  VENLRIVLTDGASHAVDSSELAFKMAAIYAFRLCYTAARPVILEPVMLVELKVPTEFQGT 675

Query: 336  VVGQLNKRXXXXXXXXXXXGWTSIYAEVPLNNMFGYAGELRSMTQGKGEFSMEYSRYSPC 157
            V G +NKR             + I A VPLNNMFGY+  LRSMTQGKGEF+MEY  +S  
Sbjct: 676  VAGDINKRKGIIVGNDQEGDDSVITANVPLNNMFGYSTSLRSMTQGKGEFTMEYKEHSAV 735

Query: 156  LPDVQEQLIRKH 121
              +VQ QL+  +
Sbjct: 736  SNEVQAQLVNAY 747


>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
            similar to mitochondrial elongation factor GI:3917 from
            [Saccharomyces cerevisiae]
          Length = 754

 Score =  122 bits (293), Expect = 2e-28
 Identities = 65/132 (49%), Positives = 80/132 (60%)
 Frame = -3

Query: 516  VSGVKFRLQDGAHHIVDSSELAFFLAAKGAVKDVFDVGAWQILEPVMFVEVTLPDEFHGT 337
            V  ++  L DGA H VDSSELAF +AA  A +  +      ILEPVM VE+ +P EF GT
Sbjct: 616  VENLRIVLTDGASHAVDSSELAFKMAAIYAFRLCYTAARPVILEPVMLVELKVPTEFQGT 675

Query: 336  VVGQLNKRXXXXXXXXXXXGWTSIYAEVPLNNMFGYAGELRSMTQGKGEFSMEYSRYSPC 157
            V G +NKR             + I A VPLNNMFGY+  LRSMTQGKGEF+MEY  +S  
Sbjct: 676  VAGDINKRKGIIVGNDQEGDDSVITANVPLNNMFGYSTSLRSMTQGKGEFTMEYKEHSAV 735

Query: 156  LPDVQEQLIRKH 121
              +VQ QL+  +
Sbjct: 736  SNEVQAQLVNAY 747


>At1g62750.1 68414.m07082 elongation factor Tu family protein similar
            to elongation factor G SP:P34811 [Glycine max (Soybean)]
          Length = 783

 Score = 89.0 bits (211), Expect = 2e-18
 Identities = 52/135 (38%), Positives = 74/135 (54%), Gaps = 1/135 (0%)
 Frame = -3

Query: 516  VSGVKFRLQDGAHHIVDSSELAFFLAAKGAVKDVFDVGAWQILEPVMFVEVTLPDEFHGT 337
            V  V+  L DG++H VDSS LAF LAA+GA ++       ++LEP+M VEV  P+E  G 
Sbjct: 646  VVDVRACLVDGSYHDVDSSVLAFQLAARGAFREGMRKAGPRMLEPIMRVEVVTPEEHLGD 705

Query: 336  VVGQLNKRXXXXXXXXXXXGWTSIY-AEVPLNNMFGYAGELRSMTQGKGEFSMEYSRYSP 160
            V+G LN R           G   +  + VPL  MF Y   LR MT+G+  ++M+ +++  
Sbjct: 706  VIGDLNSRRGQINSFGDKPGGLKVVDSLVPLAEMFQYVSTLRGMTKGRASYTMQLAKFDV 765

Query: 159  CLPDVQEQLIRKHQE 115
                +Q QL  K QE
Sbjct: 766  VPQHIQNQLSSKDQE 780


>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
            to eukaryotic translation elongation factor 2
            GB:NP_001952 [Homo sapiens]
          Length = 1015

 Score = 34.7 bits (76), Expect = 0.038
 Identities = 28/112 (25%), Positives = 50/112 (44%), Gaps = 7/112 (6%)
 Frame = -3

Query: 396  QILEPVMFVEVTLPDEFHGTVVGQLNKRXXXXXXXXXXXGWT--SIYAEVPLNNMFGYAG 223
            +I+E + F E+    E+ G +   L++R           G +  +++A VP++  FG+A 
Sbjct: 873  RIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFAD 932

Query: 222  ELRSMTQGKGEFSMEYSRY-----SPCLPDVQEQLIRKHQEEMGLLPDQKKK 82
            ELR  T G     M  S +      P      E+ I +  +   +LP+  +K
Sbjct: 933  ELRKGTSGGASALMVLSHWEMLEEDPFFVPKTEEEIEEFGDGASVLPNTARK 984


>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
           putative similar to ELONGATION FACTOR 2 GB:O14460 from
           [Schizosaccharomyces pombe]
          Length = 843

 Score = 33.1 bits (72), Expect = 0.12
 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
 Frame = -3

Query: 396 QILEPVMFVEVTLPDEFHGTVVGQLNKRXXXXXXXXXXXGWT--SIYAEVPLNNMFGYAG 223
           ++LEPV  VE+  P+   G +   LN++           G    +I A +P+   FG++ 
Sbjct: 724 RLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSS 783

Query: 222 ELRSMTQGK 196
           +LR+ T G+
Sbjct: 784 QLRAATSGQ 792


>At5g25230.1 68418.m02991 elongation factor Tu family protein
            translation Elongation Factor 2, Schizosaccharomyces
            pombe, PIR:T39902
          Length = 973

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 25/111 (22%), Positives = 47/111 (42%), Gaps = 4/111 (3%)
 Frame = -3

Query: 516  VSGVKFRLQDG--AHHIVDSSELAFFLAAKGAVKDVFDVGAWQILEPVMFVEVTLPDEFH 343
            +  VKF++ D   A   +          A+      F +   +++EPV +VE+  P +  
Sbjct: 784  IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCV 843

Query: 342  GTVVGQLNKRXXXXXXXXXXXGWTS--IYAEVPLNNMFGYAGELRSMTQGK 196
              +   L++R           G  +  + A +P+   FG+  +LR  TQG+
Sbjct: 844  TAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQ 894


>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
            to Cryptosporidium parvum elongation factor-2 GB:U21667
            GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 25/111 (22%), Positives = 47/111 (42%), Gaps = 4/111 (3%)
 Frame = -3

Query: 516  VSGVKFRLQDG--AHHIVDSSELAFFLAAKGAVKDVFDVGAWQILEPVMFVEVTLPDEFH 343
            +  VKF++ D   A   +          A+      F +   +++EPV +VE+  P +  
Sbjct: 798  IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCV 857

Query: 342  GTVVGQLNKRXXXXXXXXXXXGWTS--IYAEVPLNNMFGYAGELRSMTQGK 196
              +   L++R           G  +  + A +P+   FG+  +LR  TQG+
Sbjct: 858  TAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQ 908


>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
            to Cryptosporidium parvum elongation factor-2 GB:U21667
            GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 25/111 (22%), Positives = 47/111 (42%), Gaps = 4/111 (3%)
 Frame = -3

Query: 516  VSGVKFRLQDG--AHHIVDSSELAFFLAAKGAVKDVFDVGAWQILEPVMFVEVTLPDEFH 343
            +  VKF++ D   A   +          A+      F +   +++EPV +VE+  P +  
Sbjct: 798  IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCV 857

Query: 342  GTVVGQLNKRXXXXXXXXXXXGWTS--IYAEVPLNNMFGYAGELRSMTQGK 196
              +   L++R           G  +  + A +P+   FG+  +LR  TQG+
Sbjct: 858  TAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQ 908


>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
           GTP-binding protein GUF1 - Saccharomyces cerevisiae,
           PIR:S50374
          Length = 661

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 18/76 (23%), Positives = 32/76 (42%), Gaps = 2/76 (2%)
 Frame = -3

Query: 387 EPVMFVEVTLPDEFHGTVVGQL-NKRXXXXXXXXXXXGWTSIYAEVPLNN-MFGYAGELR 214
           EP +   + LP E+ G V+    ++R               +  ++PL   +  +  EL+
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFLKYQLPLREIVVDFYDELK 525

Query: 213 SMTQGKGEFSMEYSRY 166
           S+T G   F  E + Y
Sbjct: 526 SITSGYASFDYEDAEY 541


>At3g47660.1 68416.m05188 regulator of chromosome condensation
           (RCC1) family protein contains Pfam domain PF00415:
           Regulator of chromosome condensation (RCC1)
          Length = 951

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = +2

Query: 341 PWNSSGNVTSTNMTGSSICHAPTSKTSLTAPLAAKKN 451
           P+N    + +    GS  C++ TSK SL A +A K N
Sbjct: 648 PFNYMRKLHNCYNCGSVFCNSCTSKKSLAAAMAPKTN 684


>At3g26580.1 68416.m03318 expressed protein 
          Length = 350

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = -2

Query: 145 AGATDQEAPRGNGAATRPEEKEKLTPVEK 59
           AG++D+E+  G G     EE+E+   VEK
Sbjct: 87  AGSSDEESSDGGGGGGDEEEEEEYFDVEK 115


>At3g14590.1 68416.m01847 C2 domain-containing protein low
           similarity to SP|Q16974 Calcium-dependent protein kinase
           C (EC 2.7.1.-) {Aplysia californica}; contains Pfam
           profile PF00168: C2 domain
          Length = 737

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = -3

Query: 198 KGEFSMEYSRYSPCLPDVQEQLIRKHQEEMGL 103
           KG FS   S  SP +PD  E +  + QEE G+
Sbjct: 431 KGSFSSVVSDKSPRVPDNMEPINIEGQEETGI 462


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,656,279
Number of Sequences: 28952
Number of extensions: 169063
Number of successful extensions: 533
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 521
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 527
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)