BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS312C11f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 182 4e-47
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 180 9e-47
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 33 0.020
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.079
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 0.32
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 1.7
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 2.2
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 27 2.2
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 2.2
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 3.9
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 25 5.2
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 5.2
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 5.2
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 25 6.8
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 6.8
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 25 6.8
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 6.8
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 9.0
SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|S... 25 9.0
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 182 bits (442), Expect = 4e-47
Identities = 89/148 (60%), Positives = 110/148 (74%), Gaps = 2/148 (1%)
Frame = +3
Query: 81 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 260
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 261 RVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVF 434
R++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVF
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 435 YLKMKGDYYRYLAEVATGETRHSVVEDS 518
Y KMKGDYYRYLAE A GE R + S
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQS 150
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 180 bits (439), Expect = 9e-47
Identities = 90/149 (60%), Positives = 110/149 (73%), Gaps = 2/149 (1%)
Frame = +3
Query: 78 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 257
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 258 WRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKV 431
WR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKV
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120
Query: 432 FYLKMKGDYYRYLAEVATGETRHSVVEDS 518
FY KMKGDYYRYLAE GE + S
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSS 149
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 33.5 bits (73), Expect = 0.020
Identities = 25/104 (24%), Positives = 44/104 (42%)
Frame = +3
Query: 54 ISSLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKN 233
I S+ S S EEL+ R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2998 IMSITMSDSSAYGEELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 234 VVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREIC 365
+R+ + +E+ + EY +E+ L++ C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.079
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
Frame = +3
Query: 81 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 251
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 252 SSWRVIS 272
S+W S
Sbjct: 62 STWATFS 68
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 29.5 bits (63), Expect = 0.32
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = +3
Query: 87 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 266
+K+E QR + Q R D M + +G S+ + SV+ + +R S+
Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374
Query: 267 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 356
S E + E SE R+Q++ +E R + EK+LR
Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 1.7
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +3
Query: 381 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 518
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 2.2
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 228 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 344
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 2.2
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 3/110 (2%)
Frame = +3
Query: 87 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 257
D ++RA A QA E+ + + +K E+ +L LL V + + R S
Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322
Query: 258 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 407
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 2.2
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +3
Query: 279 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 455
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 456 YYR-YLAEV 479
+R Y+ E+
Sbjct: 100 SFRDYVGEL 108
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 3.9
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +3
Query: 216 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 350
S +Y + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 25.4 bits (53), Expect = 5.2
Identities = 30/111 (27%), Positives = 45/111 (40%), Gaps = 7/111 (6%)
Frame = +3
Query: 90 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVG-ARRSSWRV 266
KE L +L E + D + A + V NLL + YKNV A +
Sbjct: 608 KEHLYSFLQLVEPSFAKSDSSNATESQISESVRKGISIFNLLFIVYKNVCSQAGINPSTK 667
Query: 267 ISSIEQKTEGSE------RKQQMAKEYRVKVEKELREICYDVLGLLDKHLI 401
+ +++ T E + Q +EY+ K E ELR + LL+ LI
Sbjct: 668 LEDLDEHTLSDELTYITKKFVQKDQEYQTK-EIELRNYKITLQSLLEDKLI 717
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 5.2
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 25 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 147
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 5.2
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +3
Query: 300 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 422
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 25.0 bits (52), Expect = 6.8
Identities = 25/117 (21%), Positives = 45/117 (38%), Gaps = 5/117 (4%)
Frame = +3
Query: 171 TETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 350
T +G+ + +E + L V Y ++ + I + S + + ++ E
Sbjct: 278 TNSGLYSTKKELSALQVRYATLLRKFTDQTKKIEELSLAASRSSENENTIRRLALE-NHE 336
Query: 351 LREICYDVLGLLD-----KHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 506
L+ + +D KHLI ++NP+ F D Y EV T+ SV
Sbjct: 337 LKNSNNQLNNHIDDLTREKHLIALSNNPKGDEFLSPSNLDEMVYSKEVGLSFTQPSV 393
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.0 bits (52), Expect = 6.8
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +1
Query: 40 KGISELVLFHRPRCPSTRKNWCNVPNW 120
+G+ + L RC +T CNVP W
Sbjct: 694 EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1826
Score = 25.0 bits (52), Expect = 6.8
Identities = 23/79 (29%), Positives = 40/79 (50%), Gaps = 5/79 (6%)
Frame = +3
Query: 9 AGSNTFFPFRQGHQ*ISSLPSSTMSVDK---EELVQRAKL--AEQAERYDDMAAAMKEVT 173
AGS+ FP+ +GH +S S M VD+ E +V+ + A++A YD + A+ T
Sbjct: 96 AGSSFMFPYNRGHP-LSKRHDSIM-VDEFGHEYIVEGDSIASADEAIDYDALYASWTAET 153
Query: 174 ETGVELSNEERNLLSVAYK 230
+ + + E + +A K
Sbjct: 154 KAPILAIDIENIYIELAMK 172
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 6.8
Identities = 13/42 (30%), Positives = 18/42 (42%)
Frame = +3
Query: 393 HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 518
H SNP S K KG++ Y A G ++ +DS
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 24.6 bits (51), Expect = 9.0
Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
Frame = +3
Query: 372 VLGLLDKHLIPKASN-PESKVFYLKMKGD--YYRYL 470
VL L+ L+ A+ PE K+FY K GD Y +YL
Sbjct: 509 VLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYL 544
>SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein
L18|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 24.6 bits (51), Expect = 9.0
Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 2/32 (6%)
Frame = +3
Query: 384 LDKHLIPKA--SNPESKVFYLKMKGDYYRYLA 473
+++H + K+ S P S+ YLK+ YR+LA
Sbjct: 5 IERHHVKKSQRSKPASENVYLKLLVKLYRFLA 36
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,114,934
Number of Sequences: 5004
Number of extensions: 38572
Number of successful extensions: 150
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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