Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS311G04f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 34 0.050
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.12
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.12
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.12
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 0.82
At4g14830.1 68417.m02280 expressed protein 29 1.9
At5g18190.1 68418.m02135 protein kinase family protein contains ... 28 3.3
At1g76770.1 68414.m08934 heat shock protein-related contains sim... 28 4.4
At1g26140.1 68414.m03191 hypothetical protein 27 5.8
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 34.3 bits (75), Expect = 0.050
Identities = 17/39 (43%), Positives = 27/39 (69%)
Frame = +1
Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPD 519
S +F RR+ LPE A E +++ + +GVL++T P KVP+
Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPE 145
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 33.1 bits (72), Expect = 0.12
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = +1
Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPD 519
S +F+RR+ LPE A E V++ + +GVLT+ P K P+
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPE 147
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 33.1 bits (72), Expect = 0.12
Identities = 15/35 (42%), Positives = 25/35 (71%)
Frame = +1
Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 507
S +F+RR+ LPE A E V++ + +GVL++T P+
Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVPK 141
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.1 bits (72), Expect = 0.12
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +1
Query: 403 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 507
S QF RR+ LPE + V++ + +GVLT+T P+
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 30.3 bits (65), Expect = 0.82
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +1
Query: 277 DKFQVNLDVQHFSPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 456
D ++D+ EEI V+ D ++ + F R++ LPE
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 457 VESRLSSDGVLTITAPRKV 513
+ + DGVLT+ P+++
Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112
>At4g14830.1 68417.m02280 expressed protein
Length = 152
Score = 29.1 bits (62), Expect = 1.9
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +1
Query: 415 VRRYALPEGAAPETVESRLSSDGVLTITAPR 507
V R+ LPE PE V DG L +T P+
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139
>At5g18190.1 68418.m02135 protein kinase family protein contains
Pfam domains, PF00069: Protein kinase domain
Length = 691
Score = 28.3 bits (60), Expect = 3.3
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +3
Query: 213 QTVETTRGCIS*PRLQHKGRQG 278
Q T GC P + HKGRQG
Sbjct: 185 QVYNTLNGCYGVPAVHHKGRQG 206
>At1g76770.1 68414.m08934 heat shock protein-related contains
similarity to 17.9 kDa heat-shock protein [Helianthus
annuus] gi|11990130|emb|CAB55634
Length = 244
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = +1
Query: 406 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKV 513
++F + + +P+ + +++R + DG LT+T P+KV
Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV 129
>At1g26140.1 68414.m03191 hypothetical protein
Length = 162
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 240 CSRELSPRSEVVFGEHWTGDG 178
C + L P E V EHW+G+G
Sbjct: 94 CGKTLQPTMEDVKEEHWSGNG 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,103,651
Number of Sequences: 28952
Number of extensions: 213851
Number of successful extensions: 639
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 639
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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