Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS310H06f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g08750.3 68414.m00974 GPI-anchor transamidase, putative simil... 182 2e-46
At1g08750.2 68414.m00973 GPI-anchor transamidase, putative simil... 182 2e-46
At1g08750.1 68414.m00972 GPI-anchor transamidase, putative simil... 182 2e-46
At3g20210.1 68416.m02561 vacuolar processing enzyme, putative / ... 55 3e-08
At1g62710.1 68414.m07078 vacuolar processing enzyme beta / beta-... 41 6e-04
At5g10890.1 68418.m01264 myosin heavy chain-related contains wea... 34 0.050
At3g05675.2 68416.m00633 expressed protein 33 0.088
At3g05675.1 68416.m00632 expressed protein 33 0.088
At3g02470.1 68416.m00235 adenosylmethionine decarboxylase family... 27 5.8
At5g11710.1 68418.m01368 epsin N-terminal homology (ENTH) domain... 27 7.7
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 27 7.7
>At1g08750.3 68414.m00974 GPI-anchor transamidase, putative similar
to SP|P49018 GPI-anchor transamidase (EC 3.-.-.-) (GPI
transamidase) {Saccharomyces cerevisiae}; contains Pfam
profile PF01650: Peptidase C13 family
Length = 388
Score = 182 bits (442), Expect = 2e-46
Identities = 82/168 (48%), Positives = 119/168 (70%)
Frame = -3
Query: 507 PRSKQLLTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFII 328
PRSK+LL+DEGS+IL+Y+TGHGGD FLKFQD+EE+ S +LADA++QM +K+R+ E+ ++
Sbjct: 126 PRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMV 185
Query: 327 DTCQASSMYEKFYSPNILATASSLVGEDSLSHHVDSAIGVYIIDRYTYYVLEFLENVHPN 148
DTCQA++++ + SP +LA SSL GE+S SHH+DS IGV ++DR+TYY L F E ++
Sbjct: 186 DTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIY 245
Query: 147 SKRTMSEFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFFGSVRPVI 4
+++ +ST R DL+ +VP+T+FFGSV I
Sbjct: 246 DNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETI 293
>At1g08750.2 68414.m00973 GPI-anchor transamidase, putative similar
to SP|P49018 GPI-anchor transamidase (EC 3.-.-.-) (GPI
transamidase) {Saccharomyces cerevisiae}; contains Pfam
profile PF01650: Peptidase C13 family
Length = 388
Score = 182 bits (442), Expect = 2e-46
Identities = 82/168 (48%), Positives = 119/168 (70%)
Frame = -3
Query: 507 PRSKQLLTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFII 328
PRSK+LL+DEGS+IL+Y+TGHGGD FLKFQD+EE+ S +LADA++QM +K+R+ E+ ++
Sbjct: 126 PRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMV 185
Query: 327 DTCQASSMYEKFYSPNILATASSLVGEDSLSHHVDSAIGVYIIDRYTYYVLEFLENVHPN 148
DTCQA++++ + SP +LA SSL GE+S SHH+DS IGV ++DR+TYY L F E ++
Sbjct: 186 DTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIY 245
Query: 147 SKRTMSEFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFFGSVRPVI 4
+++ +ST R DL+ +VP+T+FFGSV I
Sbjct: 246 DNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETI 293
>At1g08750.1 68414.m00972 GPI-anchor transamidase, putative similar
to SP|P49018 GPI-anchor transamidase (EC 3.-.-.-) (GPI
transamidase) {Saccharomyces cerevisiae}; contains Pfam
profile PF01650: Peptidase C13 family
Length = 388
Score = 182 bits (442), Expect = 2e-46
Identities = 82/168 (48%), Positives = 119/168 (70%)
Frame = -3
Query: 507 PRSKQLLTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFII 328
PRSK+LL+DEGS+IL+Y+TGHGGD FLKFQD+EE+ S +LADA++QM +K+R+ E+ ++
Sbjct: 126 PRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMV 185
Query: 327 DTCQASSMYEKFYSPNILATASSLVGEDSLSHHVDSAIGVYIIDRYTYYVLEFLENVHPN 148
DTCQA++++ + SP +LA SSL GE+S SHH+DS IGV ++DR+TYY L F E ++
Sbjct: 186 DTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIY 245
Query: 147 SKRTMSEFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFFGSVRPVI 4
+++ +ST R DL+ +VP+T+FFGSV I
Sbjct: 246 DNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETI 293
>At3g20210.1 68416.m02561 vacuolar processing enzyme, putative /
asparaginyl endopeptidase, putative similar to
asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
GI:4589396; contains Pfam profile PF01650: Peptidase C13
family; identical to cDNA vacuolar processing enzyme
delta preproprotein (At3g20210) GI:24850432
Length = 466
Score = 55.2 bits (127), Expect = 3e-08
Identities = 34/133 (25%), Positives = 62/133 (46%), Gaps = 9/133 (6%)
Frame = -3
Query: 498 KQLLTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTC 319
K + + NI IY HG G + +EV +++ + LE+M ++K+YN++ ++ C
Sbjct: 147 KVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEAC 206
Query: 318 QASSMYEKFYSPNILATASSLVGEDSLSHHV---------DSAIGVYIIDRYTYYVLEFL 166
++ SM+E N+ A + S V S IG + D ++ LE
Sbjct: 207 ESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTFSISWLED- 265
Query: 165 ENVHPNSKRTMSE 127
++H SK T+ +
Sbjct: 266 SDLHDMSKETLEQ 278
>At1g62710.1 68414.m07078 vacuolar processing enzyme beta / beta-VPE
identical to SP|Q39044 Vacuolar processing enzyme,
beta-isozyme precursor (EC 3.4.22.-) (Beta-VPE)
{Arabidopsis thaliana}
Length = 486
Score = 40.7 bits (91), Expect = 6e-04
Identities = 20/88 (22%), Positives = 40/88 (45%), Gaps = 2/88 (2%)
Frame = -3
Query: 498 KQLLTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTC 319
K + + +I +Y HGG G L ++ + + + + L++ Y E+ ++ C
Sbjct: 152 KVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEAC 211
Query: 318 QASSMYEKFYSP--NILATASSLVGEDS 241
++ S++E NI T +S E S
Sbjct: 212 ESGSIFEGIMPKDLNIYVTTASNAQESS 239
>At5g10890.1 68418.m01264 myosin heavy chain-related contains weak
similarity to myosin heavy chain [Rana catesbeiana]
gi|4249703|gb|AAD13773
Length = 295
Score = 34.3 bits (75), Expect = 0.050
Identities = 19/61 (31%), Positives = 34/61 (55%)
Frame = -3
Query: 408 EVTSQELADALEQMWQKKRYNEIFFIIDTCQASSMYEKFYSPNILATASSLVGEDSLSHH 229
E + EL + + Y+ ++D ++S+ + +YSP I+ T+ S+ GE SLSH+
Sbjct: 106 EFHNNELRRKQLEKTETSAYSATSDVVDGPESST--DHYYSPQIIQTSMSVGGEGSLSHY 163
Query: 228 V 226
V
Sbjct: 164 V 164
>At3g05675.2 68416.m00633 expressed protein
Length = 441
Score = 33.5 bits (73), Expect = 0.088
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = -3
Query: 399 SQELADALEQMWQKKRYNEIFFIIDT-CQASSMYEKFYSPNILATASSLVGEDSLSHHVD 223
SQ AD L W + + + ++ CQ S +L S V ED+L H+V
Sbjct: 47 SQYFADRLSDKWPTCKILDSRYCVEVICQESDYDHHINLLRLLYVVSDDVHEDNLCHNVK 106
Query: 222 SAIGV 208
SA+G+
Sbjct: 107 SALGI 111
>At3g05675.1 68416.m00632 expressed protein
Length = 441
Score = 33.5 bits (73), Expect = 0.088
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = -3
Query: 399 SQELADALEQMWQKKRYNEIFFIIDT-CQASSMYEKFYSPNILATASSLVGEDSLSHHVD 223
SQ AD L W + + + ++ CQ S +L S V ED+L H+V
Sbjct: 47 SQYFADRLSDKWPTCKILDSRYCVEVICQESDYDHHINLLRLLYVVSDDVHEDNLCHNVK 106
Query: 222 SAIGV 208
SA+G+
Sbjct: 107 SALGI 111
>At3g02470.1 68416.m00235 adenosylmethionine decarboxylase family
protein contains Pfam profile: PF01536
adenosylmethionine decarboxylase
Length = 366
Score = 27.5 bits (58), Expect = 5.8
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +1
Query: 331 YEKNFIISFFLPHLFQSISKLLAGHFF*VLKLEEAVTTMAC 453
YEK ++FF P +FQ SK L +L+E +T AC
Sbjct: 11 YEKRLEVTFFEPSIFQD-SKGLGLRALTKSQLDEILTPAAC 50
>At5g11710.1 68418.m01368 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to
Af10-protein (GI:1724114) [Avena fatua]; similar to
clathrin assembly protein AP180 (GI:6492344) [Xenopus
laevis]; Af10-protein, Avena fatua, EMBL:U80041
Length = 560
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = -3
Query: 141 RTMSEFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFF 25
R + + PK A L+ VGV DL N D +K ++
Sbjct: 468 RGLIDLNITAPKKASLADVGVVGDLSNEDGNKASAASYY 506
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +2
Query: 8 TGLTEPKKSVIGTLLGSLLNKSFRTPTVD 94
TG + V+ +L G L SFR PTVD
Sbjct: 215 TGAAKAVGKVLPSLNGKLTGMSFRVPTVD 243
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,344,137
Number of Sequences: 28952
Number of extensions: 229668
Number of successful extensions: 581
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 572
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 581
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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