Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS310G07f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g49890.1 68418.m06178 chloride channel protein (CLC-c) identi... 107 4e-24
At3g27170.1 68416.m03398 chloride channel protein (CLC-b) identi... 100 5e-22
At5g26240.1 68418.m03129 chloride channel protein (CLC-d) identi... 97 5e-21
At5g40890.1 68418.m04965 chloride channel protein (CLC-a) identi... 96 1e-20
At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, pu... 94 4e-20
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 29 2.5
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 29 2.5
At3g12560.1 68416.m01563 telomeric DNA-binding protein, putative... 29 2.5
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 29 2.5
At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) ... 28 3.3
At5g21080.1 68418.m02510 expressed protein predicted proteins - ... 28 4.4
At3g07430.1 68416.m00886 YGGT family protein contains Pfam profi... 28 4.4
>At5g49890.1 68418.m06178 chloride channel protein (CLC-c) identical
to gi:1742956
Length = 779
Score = 107 bits (257), Expect = 4e-24
Identities = 56/148 (37%), Positives = 88/148 (59%), Gaps = 1/148 (0%)
Frame = +1
Query: 7 LGGVVRMTISLTVIIIETTGQISNALPIIITLVVAKWIGDFFNEGIYDIYIQLAGVPLLP 186
LGG +RMT+SL VI++E T + +++ L+++K + D FN G+YD + + G+P +
Sbjct: 528 LGGTMRMTVSLCVILLELTNNLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYME 587
Query: 187 WEPPPLAHNVYASEVMSHPVFTLRTVENVGHIVEILKLVSYNGFPVVD-PPLADDSEVTT 363
P N+ A +V+S + + VE VG I + LK+ +NGFPV+D PP + SE
Sbjct: 588 DHAEPYMRNLVAKDVVSGALISFSRVEKVGVIWQALKMTRHNGFPVIDEPPFTEASE--- 644
Query: 364 YKRLRGLILRSQLIVLLQNKIYNENANT 447
L G+ LRS L+VLLQ K +++ T
Sbjct: 645 ---LCGIALRSHLLVLLQGKKFSKQRTT 669
>At3g27170.1 68416.m03398 chloride channel protein (CLC-b) identical
to CLC-b chloride channel protein GB:CAA96058 from
[Arabidopsis thaliana] (J. Biol. Chem. 271 (52),
33632-33638 (1996))
Length = 780
Score = 100 bits (240), Expect = 5e-22
Identities = 59/145 (40%), Positives = 85/145 (58%), Gaps = 4/145 (2%)
Frame = +1
Query: 1 AQLGGVVRMTISLTVIIIETTGQISNALPI-IITLVVAKWIGDFFNEGIYDIYIQLAGVP 177
A + G +RMT+SL VI +E T + LPI +I L++AK +GD FN IYDI + L G+P
Sbjct: 515 ALMAGSMRMTVSLCVIFLELTNNLL-LLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLP 573
Query: 178 LLPWEPPPLAHNVYASEV--MSHPVFTLRTVENVGHIVEILKLVSYNGFPVVDPPLADDS 351
L P P N+ E+ PV TL+ VE V +IV++LK ++N FPV+D
Sbjct: 574 FLEANPEPWMRNLTVGELGDAKPPVVTLQGVEKVSNIVDVLKNTTHNAFPVLDEAEVPQV 633
Query: 352 EVTT-YKRLRGLILRSQLIVLLQNK 423
+ T L GLILR+ L+ +L+ +
Sbjct: 634 GLATGATELHGLILRAHLVKVLKKR 658
>At5g26240.1 68418.m03129 chloride channel protein (CLC-d) identical
to CLC-d chloride channel protein [Arabidopsis thaliana]
GI:1742959
Length = 792
Score = 97.5 bits (232), Expect = 5e-21
Identities = 55/141 (39%), Positives = 83/141 (58%), Gaps = 1/141 (0%)
Frame = +1
Query: 7 LGGVVRMTISLTVIIIETTGQISNALPIIITLVVAKWIGDFFNEGIYDIYIQLAGVPLLP 186
LGG +RMT+SL VI++E T + I++ L+++K +GD FNEG+Y++ +L G+PLL
Sbjct: 516 LGGSMRMTVSLCVIMVEITNNLKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLE 575
Query: 187 WEPPPLAHNVYASEV-MSHPVFTLRTVENVGHIVEILKLVSYNGFPVVDPPLADDSEVTT 363
P + A E S V +L V V + IL +NGFPV+D + ++ V
Sbjct: 576 SRPKYHMRQMIAKEACQSQKVISLPRVIRVADVASILGSNKHNGFPVIDHTRSGETLVI- 634
Query: 364 YKRLRGLILRSQLIVLLQNKI 426
GL+LRS L+VLLQ+K+
Sbjct: 635 -----GLVLRSHLLVLLQSKV 650
>At5g40890.1 68418.m04965 chloride channel protein (CLC-a) identical
to GI:1742952 (gb|AAC05742.1)
Length = 775
Score = 95.9 bits (228), Expect = 1e-20
Identities = 58/151 (38%), Positives = 87/151 (57%), Gaps = 4/151 (2%)
Frame = +1
Query: 7 LGGVVRMTISLTVIIIETTGQISNALPI-IITLVVAKWIGDFFNEGIYDIYIQLAGVPLL 183
+ G +RMT+SL VI +E T + LPI + L++AK +GD FN IY+I + L G+P L
Sbjct: 518 MAGSMRMTVSLCVIFLELTNNLL-LLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFL 576
Query: 184 PWEPPPLAHNVYASEV--MSHPVFTLRTVENVGHIVEILKLVSYNGFPVVDPPLADDSEV 357
P P N+ E+ PV TL VE V +IV++L+ ++N FPV+ D ++
Sbjct: 577 EANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVL-----DGADQ 631
Query: 358 TTYKRLRGLILRSQLIVLLQNKIY-NENANT 447
T L GLILR+ L+ +L+ + + NE T
Sbjct: 632 NTGTELHGLILRAHLVKVLKKRWFLNEKRRT 662
>At5g33280.1 68418.m03944 chloride channel-like (CLC) protein,
putative similar to CLC-c, At5g49890 [Arabidopsis
thaliana] and chloride channel protein ClC-1 - Nicotiana
tabacum, PIR:T02939
Length = 763
Score = 94.3 bits (224), Expect = 4e-20
Identities = 50/142 (35%), Positives = 82/142 (57%), Gaps = 1/142 (0%)
Frame = +1
Query: 7 LGGVVRMTISLTVIIIETTGQISNALPIIITLVVAKWIGDFFNEGIYDIYIQLAGVPLLP 186
LGG +RMT+S VI++E T + +++ L+++K + D FN IY++ ++L G P L
Sbjct: 500 LGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTVADGFNANIYNLIMKLKGFPYLY 559
Query: 187 WEPPPLAHNVYASEVMSHPVFTLRTVENVGHIVEILKLVSYNGFPVVD-PPLADDSEVTT 363
P + +V++ P+ +E V IV +LK ++NGFPVVD PPLA
Sbjct: 560 SHAEPYMRQLLVGDVVTGPLQVFNGIEKVETIVHVLKTTNHNGFPVVDGPPLA------A 613
Query: 364 YKRLRGLILRSQLIVLLQNKIY 429
L GLILR+ ++ LL+ +++
Sbjct: 614 APVLHGLILRAHILTLLKKRVF 635
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 28.7 bits (61), Expect = 2.5
Identities = 8/37 (21%), Positives = 23/37 (62%)
Frame = -3
Query: 432 IVYFILQQHYQLGAQYQSTKTFVCGYFGIISEGRVND 322
++ ++Q+H Q+ + ++ KT+ G+ ++S + N+
Sbjct: 61 VISILMQRHIQVDGKVRTDKTYPAGFMDVVSIPKTNE 97
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 28.7 bits (61), Expect = 2.5
Identities = 8/37 (21%), Positives = 23/37 (62%)
Frame = -3
Query: 432 IVYFILQQHYQLGAQYQSTKTFVCGYFGIISEGRVND 322
++ ++Q+H Q+ + ++ KT+ G+ ++S + N+
Sbjct: 61 VISILMQRHIQVDGKVRTDKTYPAGFMDVVSIPKTNE 97
>At3g12560.1 68416.m01563 telomeric DNA-binding protein, putative
similar to telomeric DNA-binding protein 1 [Arabidopsis
thaliana] gi|13641340|gb|AAK31590
Length = 619
Score = 28.7 bits (61), Expect = 2.5
Identities = 20/68 (29%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +1
Query: 175 PLLPWEPPPLAHNVYASEVMSHPVFTLRTVENVGHIVEI-LKLVSYNGFPVVDPPLADDS 351
P +P +P L+ AS ++ + V + +IV+ L+LV Y G VD P +D
Sbjct: 417 PAVPTDPTSLSERSAASPMLDSGIPHADDVIDSRNIVDSNLELVPYQGDISVDEPSSDSK 476
Query: 352 EVTTYKRL 375
E+ L
Sbjct: 477 ELVPLPEL 484
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 28.7 bits (61), Expect = 2.5
Identities = 8/37 (21%), Positives = 23/37 (62%)
Frame = -3
Query: 432 IVYFILQQHYQLGAQYQSTKTFVCGYFGIISEGRVND 322
++ ++Q+H Q+ + ++ KT+ G+ ++S + N+
Sbjct: 61 VISILMQRHIQVDGKVRTDKTYPAGFMDVVSIPKTNE 97
>At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) /
ferredoxin-dependent glutamate synthase (Fd-GOGAT 2)
identical to SP|Q9T0P4 Ferredoxin-dependent glutamate
synthase 2, chloroplast precursor (EC 1.4.7.1) (Fd-GOGAT
2) {Arabidopsis thaliana}
Length = 1629
Score = 28.3 bits (60), Expect = 3.3
Identities = 19/53 (35%), Positives = 28/53 (52%)
Frame = +1
Query: 169 GVPLLPWEPPPLAHNVYASEVMSHPVFTLRTVENVGHIVEILKLVSYNGFPVV 327
GVPL+ PPP H++Y+ E ++ +F L V + +KLVS G V
Sbjct: 1099 GVPLI--SPPP-HHDIYSIEDLAQLIFDLHQVNPKAKV--SVKLVSETGIGTV 1146
>At5g21080.1 68418.m02510 expressed protein predicted proteins -
Arabidopsis thaliana; expression supported by MPSS
Length = 980
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -1
Query: 140 PSLKKSPIHLATTSVIMMGRAFDICPVVSIIMT 42
PS ++S LAT+ +I +AF+I P+V+ T
Sbjct: 663 PSRRRSLFTLATSMIIFSAKAFNIPPLVNSAKT 695
>At3g07430.1 68416.m00886 YGGT family protein contains Pfam profile
PF02325: YGGT family (unknown function)
Length = 232
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/39 (28%), Positives = 24/39 (61%)
Frame = +1
Query: 91 IITLVVAKWIGDFFNEGIYDIYIQLAGVPLLPWEPPPLA 207
++ + + KW+ D ++ G+ + + L+ P +PWE PL+
Sbjct: 149 VVAVGIKKWL-DIYS-GVLMVRVLLSWFPNIPWERQPLS 185
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,457,360
Number of Sequences: 28952
Number of extensions: 257203
Number of successful extensions: 795
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 790
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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