Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS309H12f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19385.1 68415.m02261 expressed protein weak similarity to Ce... 60 7e-10
At2g19380.1 68415.m02260 RNA recognition motif (RRM)-containing ... 48 4e-06
At5g47680.1 68418.m05886 expressed protein contains Pfam profile... 29 1.4
At4g14900.1 68417.m02288 hydroxyproline-rich glycoprotein family... 29 1.4
At1g09920.1 68414.m01116 TRAF-type zinc finger-related contains ... 29 1.4
At4g16670.1 68417.m02518 expressed protein 29 1.9
At5g22020.1 68418.m02562 strictosidine synthase family protein s... 29 2.5
At5g61970.1 68418.m07778 signal recognition particle-related / S... 28 3.3
At5g36870.1 68418.m04417 glycosyl transferase family 48 protein ... 28 3.3
At2g17210.1 68415.m01987 pentatricopeptide (PPR) repeat-containi... 28 3.3
At2g32630.1 68415.m03983 pentatricopeptide (PPR) repeat-containi... 28 4.4
At3g11950.1 68416.m01473 UbiA prenyltransferase family protein c... 27 5.8
At2g34170.1 68415.m04182 expressed protein contains Pfam profile... 27 5.8
At5g42880.1 68418.m05226 hypothetical protein contains Pfam prof... 27 7.7
At5g13660.1 68418.m01590 expressed protein 27 7.7
At4g00990.1 68417.m00133 transcription factor jumonji (jmjC) dom... 27 7.7
At3g57430.1 68416.m06394 pentatricopeptide (PPR) repeat-containi... 27 7.7
At3g45900.1 68416.m04967 expressed protein 27 7.7
At2g17820.1 68415.m02064 histidine kinase 1 99% identical to GP:... 27 7.7
At1g73730.1 68414.m08537 ethylene-insensitive3-like3 (EIL3) iden... 27 7.7
>At2g19385.1 68415.m02261 expressed protein weak similarity to Cell
growth regulating nucleolar protein (Swiss-Prot:Q08288)
[Mus musculus]
Length = 275
Score = 60.5 bits (140), Expect = 7e-10
Identities = 30/64 (46%), Positives = 38/64 (59%)
Frame = +1
Query: 118 MVVFTCSHCGESVQKPKVEKHYLTKCRNKNPNLSCMDCFKDFLGKDYESHIKCITEEERY 297
MV F C CGES++KPKV H+ CR LSC+DC + F + H +CITE E+Y
Sbjct: 1 MVWFQCEDCGESLKKPKVPSHF-KMCRAN--KLSCIDCGEMFGRDTVQGHNQCITEAEKY 57
Query: 298 SGKG 309
KG
Sbjct: 58 GPKG 61
>At2g19380.1 68415.m02260 RNA recognition motif (RRM)-containing
protein similar to UBP1 interacting protein 1a
[Arabidopsis thaliana] GI:19574236; contains InterPro
entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM); contains Pfam profile PF00096:
Zinc finger, C2H2 type
Length = 613
Score = 48.0 bits (109), Expect = 4e-06
Identities = 23/60 (38%), Positives = 34/60 (56%)
Frame = +1
Query: 118 MVVFTCSHCGESVQKPKVEKHYLTKCRNKNPNLSCMDCFKDFLGKDYESHIKCITEEERY 297
MV F C CGE+++KP++ +H ++ C SC+DC F H +CITE E+Y
Sbjct: 1 MVWFQCDDCGENLKKPRLPRH-MSMC--TATKFSCIDCGNMFGQVSVHYHNQCITEAEKY 57
>At5g47680.1 68418.m05886 expressed protein contains Pfam profile
PF04243: Protein of unknown function (DUF425)
Length = 344
Score = 29.5 bits (63), Expect = 1.4
Identities = 16/59 (27%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
Frame = +1
Query: 319 KEKKGEKKQNAWVEMLQSVLDEQKSAPQNVRRII--ETISKHNNTPRKKPKFINFVKNV 489
K K+GE+K W E L + +E++ R+ + E + K + KK + +N K +
Sbjct: 58 KRKEGERKLKEWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEI 116
>At4g14900.1 68417.m02288 hydroxyproline-rich glycoprotein family
protein
Length = 532
Score = 29.5 bits (63), Expect = 1.4
Identities = 15/74 (20%), Positives = 37/74 (50%)
Frame = +1
Query: 250 KDYESHIKCITEEERYSGKGFTAKEKKGEKKQNAWVEMLQSVLDEQKSAPQNVRRIIETI 429
+D + IT++ SG+ +K + A ++ ++ E++ P+N+++ ++ +
Sbjct: 341 RDAKKATALITDDSNNSGRSAHLVARKEQSALRAVLKCIEEYKLEEEFPPENLKKRLDQL 400
Query: 430 SKHNNTPRKKPKFI 471
K T ++KP I
Sbjct: 401 EK-TKTEKRKPAVI 413
>At1g09920.1 68414.m01116 TRAF-type zinc finger-related contains
Pfam PF02176: TRAF-type zinc finger; similar to TNF
receptor associated factor 4 (GI:7274404) [Mus musculus]
(GI:7274404); similar to Cysteine rich motif Associated
to Ring and Traf domains protein (mCART1)(GI:1041446)
[Mus musculus]
Length = 192
Score = 29.5 bits (63), Expect = 1.4
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 133 CSHCGESVQKPKVEKHYLTKCRNKNPNLSCMDCFKDFLGKDYESH 267
C CG+ V K E+HYL N + ++C C + + ++SH
Sbjct: 38 CKICGDMVPKKHAEEHYL----NTHAPIACSMCKETIEREIFDSH 78
>At4g16670.1 68417.m02518 expressed protein
Length = 429
Score = 29.1 bits (62), Expect = 1.9
Identities = 22/71 (30%), Positives = 38/71 (53%), Gaps = 4/71 (5%)
Frame = +1
Query: 247 GKDYESHIKCITEEERY----SGKGFTAKEKKGEKKQNAWVEMLQSVLDEQKSAPQNVRR 414
GKD E+ TEEE Y +GKG T + K + + WV+ ++++L + V
Sbjct: 366 GKDTEN-----TEEELYFGIGTGKGLTKFKCKSKADKQTWVDSIRNLL--HRVTAVEVDT 418
Query: 415 IIETISKHNNT 447
+ETI+ +++T
Sbjct: 419 SLETININDST 429
>At5g22020.1 68418.m02562 strictosidine synthase family protein
similar to SP|P15324 Strictosidine synthase precursor
(EC 4.3.3.2) {Rauvolfia mannii}; contains Pfam profile
PF03088: Strictosidine synthase
Length = 395
Score = 28.7 bits (61), Expect = 2.5
Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 4/75 (5%)
Frame = -1
Query: 395 ALFCSSKTDC----NISTQAFCFFSPFFSLAVKPFPEYLSSSVMHLICDS*SFPRKSLKQ 228
ALF S KT ++S +C PF A+ FP ++S V+ D + R S Q
Sbjct: 2 ALFLSPKTITLLFFSLSLALYCSIDPFHHCAISDFPNFVSHEVISPRPDEVPWERDS--Q 59
Query: 227 SIHDKFGFLFLHLVR 183
+ K LF + ++
Sbjct: 60 NSLQKSKILFFNQIQ 74
>At5g61970.1 68418.m07778 signal recognition particle-related /
SRP-related low similarity to Signal recognition
particle 68 kDa protein (SRP68) from Homo sapiens
SP|Q9UHB9, Canis familiaris SP|Q00004
Length = 605
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/46 (26%), Positives = 28/46 (60%)
Frame = +1
Query: 334 EKKQNAWVEMLQSVLDEQKSAPQNVRRIIETISKHNNTPRKKPKFI 471
E + N+ +E +++E++ AP+N+ + TIS +N + + K++
Sbjct: 491 ECRANSCIEHATGIMEEER-APENLSNRMSTISLNNTAAQVEEKYL 535
>At5g36870.1 68418.m04417 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1862
Score = 28.3 bits (60), Expect = 3.3
Identities = 21/69 (30%), Positives = 30/69 (43%)
Frame = +1
Query: 103 RVLQEMVVFTCSHCGESVQKPKVEKHYLTKCRNKNPNLSCMDCFKDFLGKDYESHIKCIT 282
R + E + + H + + K K + KNP S D +DFL K K I
Sbjct: 347 RFMPECLCYIYHHMAFELFEMLESKGSKKKYKPKNPTYSGKD--EDFLTKVVTPVYKTIA 404
Query: 283 EEERYSGKG 309
EE + SG+G
Sbjct: 405 EEAKKSGEG 413
>At2g17210.1 68415.m01987 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 715
Score = 28.3 bits (60), Expect = 3.3
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = -3
Query: 498 MSTDIFDKINKLRLLSWCIVMLAYCLN 418
M+ FD+I + ++SW +++ AY +N
Sbjct: 480 MARRTFDQITEKNIISWTVIISAYAIN 506
>At2g32630.1 68415.m03983 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 624
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -1
Query: 383 SSKTDCNISTQAFCFFSPFFSLAVKPF 303
S+ DC+IS + F FF FF L + +
Sbjct: 138 SAMVDCDISEEKFEFFEKFFDLVFRVY 164
>At3g11950.1 68416.m01473 UbiA prenyltransferase family protein
contains Pfam profile PF01040: UbiA prenyltransferase
family
Length = 954
Score = 27.5 bits (58), Expect = 5.8
Identities = 22/115 (19%), Positives = 50/115 (43%), Gaps = 4/115 (3%)
Frame = +1
Query: 139 HCGESVQKPKVEKHYLTKCRNK--NPNLSCMDCFKDFLGKDYES-HIKCITEEERYSGKG 309
+C ES+ + +++H +T C K N + C D + + H+ ++ Y +
Sbjct: 226 NCSESIMRRDMDRHCITVCPMKLVNCPFHSVGCLSDVHQCEVQQHHLDNVSSHLMYILRS 285
Query: 310 FTAKEKKGEKKQNA-WVEMLQSVLDEQKSAPQNVRRIIETISKHNNTPRKKPKFI 471
+ + K A ++ L + L E ++A +++ +++ I KPK +
Sbjct: 286 IYKEASLDDLKPRAEQIQQLSTRLSEARNA-RSLTNLVKEIDGKLGPLEIKPKIV 339
>At2g34170.1 68415.m04182 expressed protein contains Pfam profile:
PF05097 protein of unknown function (DUF688)
Length = 523
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/50 (28%), Positives = 26/50 (52%)
Frame = +1
Query: 340 KQNAWVEMLQSVLDEQKSAPQNVRRIIETISKHNNTPRKKPKFINFVKNV 489
++ +V+ S ++ NV+++ ETIS+ KKPK + +K V
Sbjct: 367 EKTLYVDSENSPRTSNENRSSNVKKLPETISEEPEMEGKKPKAVRELKAV 416
>At5g42880.1 68418.m05226 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 751
Score = 27.1 bits (57), Expect = 7.7
Identities = 18/62 (29%), Positives = 26/62 (41%)
Frame = +1
Query: 280 TEEERYSGKGFTAKEKKGEKKQNAWVEMLQSVLDEQKSAPQNVRRIIETISKHNNTPRKK 459
T+E R K T +G K+N + + EQ S+ N T + +N TP K
Sbjct: 668 TDEGREPEKSPTRSSTEGRNKENGFGQSKSFAFGEQGSSSNNTGG-STTTNNNNLTPETK 726
Query: 460 PK 465
K
Sbjct: 727 KK 728
>At5g13660.1 68418.m01590 expressed protein
Length = 537
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 32 NKILIIFKFKVIHFAFKTNSPNPHVFYKKWLFLHAVI 142
NK+L ++F+ H KT PN H++++ ++ V+
Sbjct: 447 NKVLNAYEFEQ-HANCKTKHPNNHIYFENGKTIYGVV 482
>At4g00990.1 68417.m00133 transcription factor jumonji (jmjC)
domain-containing protein contains Pfam domain, PF02373:
jmjC domain
Length = 840
Score = 27.1 bits (57), Expect = 7.7
Identities = 27/92 (29%), Positives = 41/92 (44%)
Frame = +1
Query: 139 HCGESVQKPKVEKHYLTKCRNKNPNLSCMDCFKDFLGKDYESHIKCITEEERYSGKGFTA 318
H GE+V P H + RN+ SC+ DF+ + +T+E R K ++
Sbjct: 753 HLGEAVFIPAGCPHQV---RNRQ---SCIKVALDFVAPESVEECLRLTQEFRRLPKDHSS 806
Query: 319 KEKKGEKKQNAWVEMLQSVLDEQKSAPQNVRR 414
E K E K+ A + S + E K Q+ RR
Sbjct: 807 SEDKLELKKIA-LYAASSAIREVKGLMQSSRR 837
>At3g57430.1 68416.m06394 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 890
Score = 27.1 bits (57), Expect = 7.7
Identities = 8/29 (27%), Positives = 21/29 (72%)
Frame = -3
Query: 498 MSTDIFDKINKLRLLSWCIVMLAYCLNNS 412
MS +FD+I + +++W ++++AY ++ +
Sbjct: 570 MSRKVFDQIPQKNVITWNVIIMAYGMHGN 598
>At3g45900.1 68416.m04967 expressed protein
Length = 389
Score = 27.1 bits (57), Expect = 7.7
Identities = 9/36 (25%), Positives = 25/36 (69%)
Frame = +2
Query: 248 EKIMNRISSALLKKKDTLEKVSQLRRKRVRKNKMLG 355
++++NRI +++D + ++S+L R+ +++M+G
Sbjct: 107 KRLLNRIHEIEEEREDFMNRISELEREVSERDEMIG 142
>At2g17820.1 68415.m02064 histidine kinase 1 99% identical to
GP:4586626
Length = 1207
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +2
Query: 179 IISLNVGIKTQICHVWTVSKIF*EKIMNRISSALLKKKDTLEKV-SQLRRK 328
+I +++G+ ++W + ++ N+ A L K DT V ++LRRK
Sbjct: 858 VIVVDIGVLDLTTNIWKEQLNYLDRFSNKAKFAWLLKHDTSNTVKTELRRK 908
>At1g73730.1 68414.m08537 ethylene-insensitive3-like3 (EIL3)
identical to ethylene-insensitive3-like3 (EIL3)
GB:AF004215 [Arabidopsis thaliana] (Cell 89 (7),
1133-1144 (1997))
Length = 567
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +1
Query: 277 ITEEERYSGKGFTAKEKKGEKKQNAWVEML--QSVLDEQKSAPQNVRRIIETISKHNNTP 450
I + +R+ + ++K K+ W+ +L + L +Q S+ + ET + NN
Sbjct: 250 IAKIKRHVRQSKCLQDKMTAKESAIWLAVLNQEESLIQQPSSDNGNSNVTETHRRGNNAD 309
Query: 451 RKKP 462
R+KP
Sbjct: 310 RRKP 313
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,833,709
Number of Sequences: 28952
Number of extensions: 252233
Number of successful extensions: 932
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 897
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 929
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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