Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS309H11f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 124 4e-29
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 124 4e-29
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 123 8e-29
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 121 3e-28
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 48 4e-06
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 40 8e-04
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 40 0.001
At4g03190.1 68417.m00436 F-box family protein (FBL18) almost ide... 29 1.4
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 29 2.5
At3g24200.1 68416.m03037 monooxygenase family protein low simila... 28 4.4
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 28 4.4
At2g43470.1 68415.m05402 expressed protein 28 4.4
At1g79670.1 68414.m09291 wall-associated kinase, putative simila... 28 4.4
At5g45640.1 68418.m05612 subtilase family protein contains Pfam ... 27 5.8
At4g21700.1 68417.m03142 hypothetical protein 27 7.7
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 124 bits (299), Expect = 4e-29
Identities = 62/113 (54%), Positives = 76/113 (67%)
Frame = -2
Query: 466 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDL 287
PH++ IYPHGS D LP+ATMGSGSLAAM+VFEA +K + +EG KLV +AI +GIFNDL
Sbjct: 147 PHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAICSGIFNDL 206
Query: 286 GSGSNVDLCVIRNTGPAQYLRTYEEANVKGKKQGSYRYALGTTAVLKQRVIPL 128
GSGSNVD+CVI G +YLR Y E N + T VL ++ PL
Sbjct: 207 GSGSNVDICVI-TKGHKEYLRNYMEPNPRTYVSSKGYSFTKKTEVLLTKITPL 258
Score = 38.7 bits (86), Expect = 0.002
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = -1
Query: 521 YQGHIGAALVLGGVDRTGP 465
YQGH+ AALVLGGVD TGP
Sbjct: 129 YQGHVSAALVLGGVDITGP 147
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 124 bits (299), Expect = 4e-29
Identities = 62/113 (54%), Positives = 76/113 (67%)
Frame = -2
Query: 466 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDL 287
PH++ IYPHGS D LP+ATMGSGSLAAM+VFEA +K + +EG KLV +AI +GIFNDL
Sbjct: 147 PHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAICSGIFNDL 206
Query: 286 GSGSNVDLCVIRNTGPAQYLRTYEEANVKGKKQGSYRYALGTTAVLKQRVIPL 128
GSGSNVD+CVI G +YLR Y E N + T VL ++ PL
Sbjct: 207 GSGSNVDICVI-TKGHKEYLRNYMEPNPRTYVSSKGYSFTKKTEVLLTKITPL 258
Score = 38.7 bits (86), Expect = 0.002
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = -1
Query: 521 YQGHIGAALVLGGVDRTGP 465
YQGH+ AALVLGGVD TGP
Sbjct: 129 YQGHVSAALVLGGVDITGP 147
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 123 bits (296), Expect = 8e-29
Identities = 61/113 (53%), Positives = 76/113 (67%)
Frame = -2
Query: 466 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDL 287
PH++ IYPHGS D LP+ATMGSGSLAAM+VFEA +K + +EG KLV ++I +GIFNDL
Sbjct: 147 PHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAESICSGIFNDL 206
Query: 286 GSGSNVDLCVIRNTGPAQYLRTYEEANVKGKKQGSYRYALGTTAVLKQRVIPL 128
GSGSNVD+CVI G +YLR Y E N + T VL ++ PL
Sbjct: 207 GSGSNVDICVI-TKGNKEYLRNYMEPNPRTYVSSKGYSFTKKTEVLLTKITPL 258
Score = 38.7 bits (86), Expect = 0.002
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = -1
Query: 521 YQGHIGAALVLGGVDRTGP 465
YQGH+ AALVLGGVD TGP
Sbjct: 129 YQGHVSAALVLGGVDITGP 147
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 121 bits (291), Expect = 3e-28
Identities = 56/87 (64%), Positives = 68/87 (78%)
Frame = -2
Query: 466 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDL 287
PH++ IYPHGS D LP+ATMGSGSLAAM+VFEA +K + +EG KLV ++I +GIFNDL
Sbjct: 147 PHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAESICSGIFNDL 206
Query: 286 GSGSNVDLCVIRNTGPAQYLRTYEEAN 206
GSGSNVD+CVI G +YLR Y E N
Sbjct: 207 GSGSNVDICVI-TKGNKEYLRNYMEPN 232
Score = 38.7 bits (86), Expect = 0.002
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = -1
Query: 521 YQGHIGAALVLGGVDRTGP 465
YQGH+ AALVLGGVD TGP
Sbjct: 129 YQGHVSAALVLGGVDITGP 147
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 48.0 bits (109), Expect = 4e-06
Identities = 27/73 (36%), Positives = 39/73 (53%)
Frame = -2
Query: 460 IYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDLGS 281
IY I G+V + P+A GSGS F+ WK +M +EE ++LV A++ I D S
Sbjct: 123 IYGIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGAS 182
Query: 280 GSNVDLCVIRNTG 242
G V +I + G
Sbjct: 183 GGVVRTVIINSEG 195
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 40.3 bits (90), Expect = 8e-04
Identities = 24/66 (36%), Positives = 34/66 (51%)
Frame = -2
Query: 475 EPVPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIF 296
E P +Y + G K ++GSGS A V ++G+K DM+ EE +L R +I F
Sbjct: 163 ETGPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATF 222
Query: 295 NDLGSG 278
D SG
Sbjct: 223 RDGASG 228
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 39.9 bits (89), Expect = 0.001
Identities = 24/66 (36%), Positives = 34/66 (51%)
Frame = -2
Query: 475 EPVPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIF 296
E P +Y + G K ++GSGS A V ++G+K DM+ EE +L R +I F
Sbjct: 163 ETGPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATF 222
Query: 295 NDLGSG 278
D SG
Sbjct: 223 RDGASG 228
>At4g03190.1 68417.m00436 F-box family protein (FBL18) almost
identical to GRR1-like protein 1 GI:12658970 from
[Arabidopsis thaliana]; similar to leucine-rich repeats
containing F-box protein FBL3 (GI:5919219) [Homo
sapiens]; similar to F-box protein FBL2 (GI:6063090)
[Homo sapiens]
Length = 585
Score = 29.5 bits (63), Expect = 1.4
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 4/69 (5%)
Frame = -2
Query: 517 KAI*GLLWFWEVLTEPVPHIYCIYPHGSVDKLPYATMGSGSLAAM----AVFEAGWKRDM 350
K + L W+VL E +P +Y + P + L YAT+ L + + + W D+
Sbjct: 261 KQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYATVRMPDLVELLRRCSKLQKLWVMDL 320
Query: 349 NEEEGKKLV 323
E++G + V
Sbjct: 321 IEDKGLEAV 329
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 28.7 bits (61), Expect = 2.5
Identities = 21/85 (24%), Positives = 41/85 (48%)
Frame = -2
Query: 466 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDL 287
P +Y P G+ + +GSGS A + + + +D++ +E + + +I + +
Sbjct: 149 PSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDLSLQEA-ETIAVSILKQVMEEK 207
Query: 286 GSGSNVDLCVIRNTGPAQYLRTYEE 212
+ +NVD I PA +L T +E
Sbjct: 208 VTPNNVD---IAKVAPAYHLYTPQE 229
>At3g24200.1 68416.m03037 monooxygenase family protein low
similarity to VisC [Escherichia coli][GI:216629];
contains Pfam profile: PF01360 Monooxygenase
Length = 507
Score = 27.9 bits (59), Expect = 4.4
Identities = 16/48 (33%), Positives = 25/48 (52%)
Frame = -2
Query: 445 PHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAG 302
P+G + LP S + M EA ++ MNE++ K V DA+ +G
Sbjct: 285 PNGPIALLPIGDKFSNIVWTMDPKEASDRKLMNEDDFIKAVNDALDSG 332
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 27.9 bits (59), Expect = 4.4
Identities = 21/85 (24%), Positives = 40/85 (47%)
Frame = -2
Query: 466 PHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFNDL 287
P +Y P G+ + +GSGS A + + + +D+ +E + + +I + +
Sbjct: 149 PSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDITLQEA-ETIAVSILKQVMEEK 207
Query: 286 GSGSNVDLCVIRNTGPAQYLRTYEE 212
+ +NVD I PA +L T +E
Sbjct: 208 VTPNNVD---IAKVAPAYHLYTPQE 229
>At2g43470.1 68415.m05402 expressed protein
Length = 498
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = -2
Query: 196 KKQGSYRYALGTTAVLKQRVIPLEVESILVRPTEAFQP 83
KK+ LGTT V + ++ + + S + +P++ FQP
Sbjct: 85 KKKKEDHNGLGTTRVDNEEIVNMVLASQVYQPSQVFQP 122
>At1g79670.1 68414.m09291 wall-associated kinase, putative similar
to wall-associated kinase 4 GI:3355308 from [Arabidopsis
thaliana]; isoform contains non-consensus AT-acceptor
splice site.
Length = 751
Score = 27.9 bits (59), Expect = 4.4
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -1
Query: 206 CEGEKARFV*ICSWYNCC 153
C+G+K+R IC Y CC
Sbjct: 166 CDGDKSRLDKICGGYTCC 183
>At5g45640.1 68418.m05612 subtilase family protein contains Pfam
domain, PF00082: Subtilase family; contains Pfam domain,
PF02225: protease associated (PA) domain
Length = 754
Score = 27.5 bits (58), Expect = 5.8
Identities = 19/61 (31%), Positives = 25/61 (40%), Gaps = 1/61 (1%)
Frame = -2
Query: 406 GSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAIAAGIFND-LGSGSNVDLCVIRNTGPAQY 230
G SLA +AV++A W E+ D F+D + G NV I P Y
Sbjct: 228 GGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAFDDAIADGVNVISISIGTVEPHTY 287
Query: 229 L 227
L
Sbjct: 288 L 288
>At4g21700.1 68417.m03142 hypothetical protein
Length = 962
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
Frame = +2
Query: 287 QVIEYTSSNSISDKLFSFFLIHITFPTCFKHCHSRKRSRSHGCI--W*FIYRSMRIN 451
Q++ SNS L + F + +F H + RS S+G I W FIY + +++
Sbjct: 845 QILFNGFSNSDLKPLAALFYVGNSFVRLLPHAYDLYRSHSYGKILDWSFIYANHKMD 901
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,201,857
Number of Sequences: 28952
Number of extensions: 222862
Number of successful extensions: 575
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 565
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 575
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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