BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS309A10f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g51490.1 68418.m06386 pectinesterase family protein contains ... 29 1.4 At2g25850.2 68415.m03104 nucleotidyltransferase family protein c... 28 4.4 At2g25850.1 68415.m03103 nucleotidyltransferase family protein c... 28 4.4 At4g00300.1 68417.m00037 fringe-related protein + weak similarit... 27 5.8 At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 27 7.7 >At5g51490.1 68418.m06386 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 536 Score = 29.5 bits (63), Expect = 1.4 Identities = 15/42 (35%), Positives = 23/42 (54%) Frame = -1 Query: 482 GRSSASTTEFVHSSSNLVKVSAKSWNCPCLLGALLVSGFKGS 357 G S + T+F+ + K+S NC + GALL +G KG+ Sbjct: 154 GSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTAGNKGN 195 >At2g25850.2 68415.m03104 nucleotidyltransferase family protein contains Pfam profiles: PF01909 nucleotidyltransferase domain, PF04926 poly(A) polymerase predicted RNA binding domain; identical to cDNA GI:31747890 Length = 800 Score = 27.9 bits (59), Expect = 4.4 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Frame = -2 Query: 223 QVTLNLLQDLD--LARRPVPRTLGPATLKFSDCPFYLGLRRTD 101 Q+TL + +D + L P P + +F C F++GL+R D Sbjct: 404 QLTLKIERDTNGMLMCHPQPNEYVDTSKQFRHCAFFMGLQRAD 446 >At2g25850.1 68415.m03103 nucleotidyltransferase family protein contains Pfam profiles: PF01909 nucleotidyltransferase domain, PF04926 poly(A) polymerase predicted RNA binding domain; identical to cDNA GI:31747890 Length = 787 Score = 27.9 bits (59), Expect = 4.4 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Frame = -2 Query: 223 QVTLNLLQDLD--LARRPVPRTLGPATLKFSDCPFYLGLRRTD 101 Q+TL + +D + L P P + +F C F++GL+R D Sbjct: 404 QLTLKIERDTNGMLMCHPQPNEYVDTSKQFRHCAFFMGLQRAD 446 >At4g00300.1 68417.m00037 fringe-related protein + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 785 Score = 27.5 bits (58), Expect = 5.8 Identities = 16/55 (29%), Positives = 21/55 (38%) Frame = +1 Query: 187 LNPGPGVGSKSPGDNHYRLIVNGEVERYAPDQRYVVTLVGSRTHDVVQQFAGFKI 351 L G G KS + G PD Y VG R++ +V GFK+ Sbjct: 550 LKTGDGSSEKSYFTGRIKYRRGGASREVGPDNVYNTARVGKRSNGLVDMSWGFKV 604 >At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1 [Mus musculus] GI:3228668; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 307 Score = 27.1 bits (57), Expect = 7.7 Identities = 17/75 (22%), Positives = 32/75 (42%) Frame = +1 Query: 199 PGVGSKSPGDNHYRLIVNGEVERYAPDQRYVVTLVGSRTHDVVQQFAGFKIILDPLNPDT 378 P +K G+ H+ +++ R Q YV+ + H+ ++ G +I+DP Sbjct: 212 PSAFTKVTGEAHWEILLRA---RAIETQCYVIAAAQAGKHNEKRESYGDTLIIDPWGTVV 268 Query: 379 RRAPSKQGQFQLFAD 423 R P + + AD Sbjct: 269 GRLPDRVSTGIVVAD 283 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.316 0.136 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,516,673 Number of Sequences: 28952 Number of extensions: 188872 Number of successful extensions: 499 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 493 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 499 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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