BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS308F04f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 72 6e-14 SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizo... 29 0.56 SPAC926.08c |||Brix doamin protein Rpf2 |Schizosaccharomyces pom... 26 3.9 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 3.9 SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Sc... 25 5.2 SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Ma... 25 6.8 >SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 261 Score = 71.7 bits (168), Expect = 6e-14 Identities = 35/106 (33%), Positives = 56/106 (52%), Gaps = 5/106 (4%) Frame = +3 Query: 210 VPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQL 389 VPR SYF ++ ++R ++ SE WLD+NG PLKWH+P+G L+DL DP Sbjct: 36 VPRQSYFAQILPNVQR----LLAPSIPLSECWLDYNGVPLKWHWPVGLLFDLLTVFDPDT 91 Query: 390 P-----WTLTVHFTKFPEDILLHCPNKDVVEAHYMSTVKEADVLKH 512 P W + + FP +L D ++ + +KE+D +++ Sbjct: 92 PRAPVLWRIQLRSGLFPTTKILQMETMDTFRTYFFNCLKESDYVRN 137 >SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 28.7 bits (61), Expect = 0.56 Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%) Frame = +3 Query: 171 IMEIQQPDP-FYVMVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFN 317 I E P+P ++ +P+L++ V+DK K R I N E+ L N Sbjct: 140 IKEPTAPEPDCHIFLPQLNFLRHVVDKYKSLSDRIIMSANMSGELQLSVN 189 >SPAC926.08c |||Brix doamin protein Rpf2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 25.8 bits (54), Expect = 3.9 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = -1 Query: 221 KSRHHNVKWIRLLNFHYFFLSKMKTYR*FSIPYFSKYLPVV 99 K R HN+ W+R N+ + ++ SI FS P+V Sbjct: 94 KKRPHNLTWVRFFNYRVLDMIELGIVNYKSIQSFSA-TPIV 133 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 25.8 bits (54), Expect = 3.9 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = -1 Query: 500 ISLFHCRHIMCFYYIFVWAMKKNVFRELCE 411 + LF +++ +Y I V K N+F ELC+ Sbjct: 1173 LELFDHPNVVSYYGIEVHREKVNIFMELCQ 1202 >SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 25.4 bits (53), Expect = 5.2 Identities = 16/42 (38%), Positives = 22/42 (52%) Frame = -3 Query: 504 VHQPLSL*TYNVLLLHLCLGNEEECLQGTL*SVLSKSRVVED 379 V LS TYNVL LC+ E+ L T +VL+ ++ D Sbjct: 241 VFPTLSQDTYNVLRACLCVSPEKRSLAKTREAVLAVTKWTTD 282 >SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 387 Score = 25.0 bits (52), Expect = 6.8 Identities = 10/41 (24%), Positives = 21/41 (51%) Frame = -2 Query: 136 FPSHISRSTSLSLAILIVINMLITEHYYFLMLIVDALQPIN 14 FP H + + +L+++ + Y L+ I++ QPI+ Sbjct: 8 FPLHHKLALPFRIGLLVIVGTWLWSVCYHLIYILNRYQPIS 48 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,259,007 Number of Sequences: 5004 Number of extensions: 49457 Number of successful extensions: 123 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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