BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS308F04f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17290.1 68418.m02025 autophagy protein Apg5 family contains ... 113 5e-26 At1g74800.1 68414.m08666 galactosyltransferase family protein co... 30 0.82 At1g10040.1 68414.m01132 expressed protein non-consensus GC dono... 29 1.9 At4g01720.1 68417.m00223 WRKY family transcription factor simila... 28 3.3 At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containi... 28 4.4 At2g23360.1 68415.m02790 transport protein-related contains Pfam... 27 5.8 At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family p... 27 7.7 At5g35740.1 68418.m04280 glycosyl hydrolase family protein 17 si... 27 7.7 At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 7.7 At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 27 7.7 At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 27 7.7 At2g33820.1 68415.m04149 mitochondrial substrate carrier family ... 27 7.7 At2g14590.1 68415.m01635 hypothetical protein 27 7.7 >At5g17290.1 68418.m02025 autophagy protein Apg5 family contains Pfam profile: PF04106 autophagy protein Apg5 Length = 337 Score = 113 bits (273), Expect = 5e-26 Identities = 47/134 (35%), Positives = 82/134 (61%) Frame = +3 Query: 105 REVLREIWDGKLPICFHLAQEEIMEIQQPDPFYVMVPRLSYFPLVIDKMKRHFLRFISQE 284 +E ++ +W+G +P+ HL + ++ P P V+ PR+ Y PL+I +K +F S Sbjct: 3 KEAVKYVWEGAIPLQIHLHKSDVASHPAPPPALVLAPRIGYLPLLIPLIKPYFKD--SLP 60 Query: 285 NSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQLPWTLTVHFTKFPEDILLHCPNKDVV 464 + +W D+ G PLKW+ P G L+DL C +P+ PW LT+HF +P +IL+ C +D V Sbjct: 61 PGEDSIWFDYKGFPLKWYIPTGVLFDLLCA-EPERPWNLTIHFRGYPCNILIPCEGEDSV 119 Query: 465 EAHYMSTVKEADVL 506 + ++++++KEA + Sbjct: 120 KWNFVNSLKEAQYI 133 >At1g74800.1 68414.m08666 galactosyltransferase family protein contains Pfam profile: PF01762 galactosyltransferase Length = 672 Score = 30.3 bits (65), Expect = 0.82 Identities = 17/60 (28%), Positives = 28/60 (46%) Frame = +3 Query: 132 GKLPICFHLAQEEIMEIQQPDPFYVMVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLD 311 GK + + EE P YV+ ++ F ++DK +RH LR E+ MW++ Sbjct: 559 GKWAVTYEEWPEEDYPPYANGPGYVLSSDIARF--IVDKFERHKLRLFKMEDVSVGMWVE 616 >At1g10040.1 68414.m01132 expressed protein non-consensus GC donor splice site at exon boundary 21576 Length = 412 Score = 29.1 bits (62), Expect = 1.9 Identities = 20/84 (23%), Positives = 39/84 (46%) Frame = +3 Query: 264 LRFISQENSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQLPWTLTVHFTKFPEDILLH 443 LR S E+ + ++ +DF G+ + + + + L G+ + K+P+D+L+H Sbjct: 56 LRAESDESGNVDLTVDFPGERTEPTHLVVMVNGLI-GSAQNWRFAAKQMLKKYPQDLLVH 114 Query: 444 CPNKDVVEAHYMSTVKEADVLKHR 515 C ++ H T DV+ R Sbjct: 115 CSKRN----HSTQTFDGVDVMGER 134 >At4g01720.1 68417.m00223 WRKY family transcription factor similar to wild oat DNA-binding protein ABF2, GenBank accession number Z48431 Length = 489 Score = 28.3 bits (60), Expect = 3.3 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +3 Query: 441 HCPNKDVVEAHYMSTVKEADVLKHRGQ 521 H PN+ VE H+ S++KE D + Q Sbjct: 29 HQPNESPVERHHESSIKEVDFFAAKSQ 55 >At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containing protein low similarity to fertility restorer [Petunia x hybrida] GI:22128587; contains Pfam profile PF01535: PPR repeat Length = 1052 Score = 27.9 bits (59), Expect = 4.4 Identities = 17/70 (24%), Positives = 35/70 (50%), Gaps = 2/70 (2%) Frame = +3 Query: 168 EIMEIQQPDPFYV--MVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFNGQPLKWHY 341 EI E + +PF++ ++P +++ V ++RH+L N ++ + F+G HY Sbjct: 518 EIWERSKSNPFWMQRLIP-IAFSSSVKGFVRRHYLLLERGNNPETSLSRSFSGASHHHHY 576 Query: 342 PIGFLYDLYC 371 +L+C Sbjct: 577 RERLRNELHC 586 >At2g23360.1 68415.m02790 transport protein-related contains Pfam PF05911: Plant protein of unknown function (DUF869) profile; weak similarity to Intracellular protein transport protein USO1 (Swiss-Prot:P25386) [Saccharomyces cerevisiae] Length = 886 Score = 27.5 bits (58), Expect = 5.8 Identities = 11/33 (33%), Positives = 21/33 (63%) Frame = +2 Query: 68 DQHINNY*NGQRQGGTSRNMGWKTTDMFSSCSR 166 ++H++N + + G T+R + WKTT++ S R Sbjct: 555 ERHVSNRESERLSGYTARVLQWKTTELSSVLQR 587 >At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family protein similar to SP|Q9Y672 Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC 2.4.1.-) (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase) {Homo sapiens}; contains Pfam profile PF03155: ALG6, ALG8 glycosyltransferase family Length = 533 Score = 27.1 bits (57), Expect = 7.7 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Frame = -3 Query: 174 LFLLEQDENISVVFHPIFLEV-PPCRWPF 91 +FLL ISVV H ++L + PP ++PF Sbjct: 463 VFLLRTHFFISVVLHVLYLTIKPPQKYPF 491 >At5g35740.1 68418.m04280 glycosyl hydrolase family protein 17 similar to elicitor inducible chitinase Nt-SubE76 GI:11071974 from [Nicotiana tabacum]; C-terminal homology only Length = 119 Score = 27.1 bits (57), Expect = 7.7 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -2 Query: 418 FVKCTVKVQGS*GSLPQYKSYKNPMG*CHFKGCPL 314 F+ T++ S Y++YKN G C+FKG + Sbjct: 68 FLPNTIRDHASFAFNSYYQTYKNKGGSCYFKGAAM 102 >At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box RNA helicase [Chlamydomonas reinhardtii] GI:12044832; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 1226 Score = 27.1 bits (57), Expect = 7.7 Identities = 16/59 (27%), Positives = 28/59 (47%), Gaps = 3/59 (5%) Frame = +3 Query: 339 YPIGFLYDLY-CGN--DPQLPWTLTVHFTKFPEDILLHCPNKDVVEAHYMSTVKEADVL 506 +P+ LY C + + + +T+H T P DIL+ +D +EA S + + L Sbjct: 706 FPVNILYSKTPCEDYVEAAVKQAMTIHITSPPGDILIFMTGQDEIEAACFSLKERMEQL 764 >At3g60860.1 68416.m06808 guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor, Homo sapiens, GI:5456754; contains Pfam profile PF01369: Sec7 domain Length = 1793 Score = 27.1 bits (57), Expect = 7.7 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = -3 Query: 456 LCLGNEEECLQGTL*SVLSKSRVVEDHYHNTNRTKILWDS 337 LC + +E + V S +++VE ++N NR +++W S Sbjct: 1093 LCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSS 1132 >At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase domain-containing protein / F-box family protein similar to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces pombe}; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, PF00646: F-box domain Length = 1378 Score = 27.1 bits (57), Expect = 7.7 Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 2/34 (5%) Frame = -1 Query: 410 VYCQSPG*LRIITTIQIVQKSYGIV--PFQGLPI 315 ++C PG + IT + ++ K+ G + P +GLPI Sbjct: 391 MFCDEPGLGKTITALSLILKTQGTMADPPEGLPI 424 >At2g33820.1 68415.m04149 mitochondrial substrate carrier family protein (BAC1) contains Pfam profile: PF00153 mitochondrial carrier protein Length = 311 Score = 27.1 bits (57), Expect = 7.7 Identities = 8/24 (33%), Positives = 17/24 (70%) Frame = -2 Query: 415 VKCTVKVQGS*GSLPQYKSYKNPM 344 VKC +++QG+ +P ++ Y +P+ Sbjct: 137 VKCRMQIQGTDSLVPNFRRYNSPL 160 >At2g14590.1 68415.m01635 hypothetical protein Length = 323 Score = 27.1 bits (57), Expect = 7.7 Identities = 8/22 (36%), Positives = 15/22 (68%) Frame = -3 Query: 387 VEDHYHNTNRTKILWDSAISRV 322 + D Y N++ T+ WDS ++R+ Sbjct: 135 ISDKYWNSSETRAAWDSVVTRM 156 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,559,788 Number of Sequences: 28952 Number of extensions: 249278 Number of successful extensions: 558 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 547 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 556 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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