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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS308F04f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g17290.1 68418.m02025 autophagy protein Apg5 family contains ...   113   5e-26
At1g74800.1 68414.m08666 galactosyltransferase family protein co...    30   0.82 
At1g10040.1 68414.m01132 expressed protein non-consensus GC dono...    29   1.9  
At4g01720.1 68417.m00223 WRKY family transcription factor simila...    28   3.3  
At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containi...    28   4.4  
At2g23360.1 68415.m02790 transport protein-related contains Pfam...    27   5.8  
At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family p...    27   7.7  
At5g35740.1 68418.m04280 glycosyl hydrolase family protein 17 si...    27   7.7  
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-...    27   7.7  
At3g60860.1 68416.m06808 guanine nucleotide exchange family prot...    27   7.7  
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica...    27   7.7  
At2g33820.1 68415.m04149 mitochondrial substrate carrier family ...    27   7.7  
At2g14590.1 68415.m01635 hypothetical protein                          27   7.7  

>At5g17290.1 68418.m02025 autophagy protein Apg5 family contains
           Pfam profile: PF04106 autophagy protein Apg5
          Length = 337

 Score =  113 bits (273), Expect = 5e-26
 Identities = 47/134 (35%), Positives = 82/134 (61%)
 Frame = +3

Query: 105 REVLREIWDGKLPICFHLAQEEIMEIQQPDPFYVMVPRLSYFPLVIDKMKRHFLRFISQE 284
           +E ++ +W+G +P+  HL + ++     P P  V+ PR+ Y PL+I  +K +F    S  
Sbjct: 3   KEAVKYVWEGAIPLQIHLHKSDVASHPAPPPALVLAPRIGYLPLLIPLIKPYFKD--SLP 60

Query: 285 NSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQLPWTLTVHFTKFPEDILLHCPNKDVV 464
             +  +W D+ G PLKW+ P G L+DL C  +P+ PW LT+HF  +P +IL+ C  +D V
Sbjct: 61  PGEDSIWFDYKGFPLKWYIPTGVLFDLLCA-EPERPWNLTIHFRGYPCNILIPCEGEDSV 119

Query: 465 EAHYMSTVKEADVL 506
           + ++++++KEA  +
Sbjct: 120 KWNFVNSLKEAQYI 133


>At1g74800.1 68414.m08666 galactosyltransferase family protein
           contains Pfam profile: PF01762 galactosyltransferase
          Length = 672

 Score = 30.3 bits (65), Expect = 0.82
 Identities = 17/60 (28%), Positives = 28/60 (46%)
 Frame = +3

Query: 132 GKLPICFHLAQEEIMEIQQPDPFYVMVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLD 311
           GK  + +    EE        P YV+   ++ F  ++DK +RH LR    E+    MW++
Sbjct: 559 GKWAVTYEEWPEEDYPPYANGPGYVLSSDIARF--IVDKFERHKLRLFKMEDVSVGMWVE 616


>At1g10040.1 68414.m01132 expressed protein non-consensus GC donor
           splice site at exon boundary 21576
          Length = 412

 Score = 29.1 bits (62), Expect = 1.9
 Identities = 20/84 (23%), Positives = 39/84 (46%)
 Frame = +3

Query: 264 LRFISQENSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQLPWTLTVHFTKFPEDILLH 443
           LR  S E+ + ++ +DF G+  +  + +  +  L  G+     +       K+P+D+L+H
Sbjct: 56  LRAESDESGNVDLTVDFPGERTEPTHLVVMVNGLI-GSAQNWRFAAKQMLKKYPQDLLVH 114

Query: 444 CPNKDVVEAHYMSTVKEADVLKHR 515
           C  ++    H   T    DV+  R
Sbjct: 115 CSKRN----HSTQTFDGVDVMGER 134


>At4g01720.1 68417.m00223 WRKY family transcription factor similar
           to wild oat DNA-binding protein ABF2, GenBank accession
           number Z48431
          Length = 489

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = +3

Query: 441 HCPNKDVVEAHYMSTVKEADVLKHRGQ 521
           H PN+  VE H+ S++KE D    + Q
Sbjct: 29  HQPNESPVERHHESSIKEVDFFAAKSQ 55


>At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containing
           protein low similarity to fertility restorer [Petunia x
           hybrida] GI:22128587; contains Pfam profile PF01535: PPR
           repeat
          Length = 1052

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 17/70 (24%), Positives = 35/70 (50%), Gaps = 2/70 (2%)
 Frame = +3

Query: 168 EIMEIQQPDPFYV--MVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFNGQPLKWHY 341
           EI E  + +PF++  ++P +++   V   ++RH+L      N ++ +   F+G     HY
Sbjct: 518 EIWERSKSNPFWMQRLIP-IAFSSSVKGFVRRHYLLLERGNNPETSLSRSFSGASHHHHY 576

Query: 342 PIGFLYDLYC 371
                 +L+C
Sbjct: 577 RERLRNELHC 586


>At2g23360.1 68415.m02790 transport protein-related contains Pfam
           PF05911: Plant protein of unknown function (DUF869)
           profile; weak similarity to Intracellular protein
           transport protein USO1 (Swiss-Prot:P25386)
           [Saccharomyces cerevisiae]
          Length = 886

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = +2

Query: 68  DQHINNY*NGQRQGGTSRNMGWKTTDMFSSCSR 166
           ++H++N  + +  G T+R + WKTT++ S   R
Sbjct: 555 ERHVSNRESERLSGYTARVLQWKTTELSSVLQR 587


>At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family
           protein similar to SP|Q9Y672 Dolichyl pyrophosphate
           Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC 2.4.1.-)
           (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl
           glucosyltransferase) {Homo sapiens}; contains Pfam
           profile PF03155: ALG6, ALG8 glycosyltransferase family
          Length = 533

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
 Frame = -3

Query: 174 LFLLEQDENISVVFHPIFLEV-PPCRWPF 91
           +FLL     ISVV H ++L + PP ++PF
Sbjct: 463 VFLLRTHFFISVVLHVLYLTIKPPQKYPF 491


>At5g35740.1 68418.m04280 glycosyl hydrolase family protein 17
           similar to elicitor inducible chitinase Nt-SubE76
           GI:11071974 from [Nicotiana tabacum]; C-terminal
           homology only
          Length = 119

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = -2

Query: 418 FVKCTVKVQGS*GSLPQYKSYKNPMG*CHFKGCPL 314
           F+  T++   S      Y++YKN  G C+FKG  +
Sbjct: 68  FLPNTIRDHASFAFNSYYQTYKNKGGSCYFKGAAM 102


>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
           RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
           contains Pfam profiles PF04408: Helicase associated
           domain (HA2), PF00271: Helicase conserved C-terminal
           domain
          Length = 1226

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 16/59 (27%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
 Frame = +3

Query: 339 YPIGFLYDLY-CGN--DPQLPWTLTVHFTKFPEDILLHCPNKDVVEAHYMSTVKEADVL 506
           +P+  LY    C +  +  +   +T+H T  P DIL+    +D +EA   S  +  + L
Sbjct: 706 FPVNILYSKTPCEDYVEAAVKQAMTIHITSPPGDILIFMTGQDEIEAACFSLKERMEQL 764


>At3g60860.1 68416.m06808 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor, Homo
            sapiens, GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1793

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 12/40 (30%), Positives = 23/40 (57%)
 Frame = -3

Query: 456  LCLGNEEECLQGTL*SVLSKSRVVEDHYHNTNRTKILWDS 337
            LC  + +E    +   V S +++VE  ++N NR +++W S
Sbjct: 1093 LCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSS 1132


>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
           domain-containing protein / F-box family protein similar
           to SP|P36607 DNA repair protein rad8
           {Schizosaccharomyces pombe}; contains Pfam profiles
           PF00271: Helicase conserved C-terminal domain, PF00176:
           SNF2 family N-terminal domain, PF00646: F-box domain
          Length = 1378

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
 Frame = -1

Query: 410 VYCQSPG*LRIITTIQIVQKSYGIV--PFQGLPI 315
           ++C  PG  + IT + ++ K+ G +  P +GLPI
Sbjct: 391 MFCDEPGLGKTITALSLILKTQGTMADPPEGLPI 424


>At2g33820.1 68415.m04149 mitochondrial substrate carrier family
           protein (BAC1) contains Pfam profile: PF00153
           mitochondrial carrier protein
          Length = 311

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 8/24 (33%), Positives = 17/24 (70%)
 Frame = -2

Query: 415 VKCTVKVQGS*GSLPQYKSYKNPM 344
           VKC +++QG+   +P ++ Y +P+
Sbjct: 137 VKCRMQIQGTDSLVPNFRRYNSPL 160


>At2g14590.1 68415.m01635 hypothetical protein
          Length = 323

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = -3

Query: 387 VEDHYHNTNRTKILWDSAISRV 322
           + D Y N++ T+  WDS ++R+
Sbjct: 135 ISDKYWNSSETRAAWDSVVTRM 156


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,559,788
Number of Sequences: 28952
Number of extensions: 249278
Number of successful extensions: 558
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 547
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 556
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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