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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS308B12f
         (511 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             116   1e-26
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)                94   7e-20
SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)                   29   1.7  
SB_10791| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.2  
SB_58170| Best HMM Match : Spindle_assoc (HMM E-Value=2.3)             29   2.9  
SB_43952| Best HMM Match : Spindle_assoc (HMM E-Value=2.3)             29   2.9  
SB_27442| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.9  
SB_5367| Best HMM Match : wnt (HMM E-Value=0)                          29   2.9  
SB_45399| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   3.9  
SB_5714| Best HMM Match : CBM_X (HMM E-Value=1.9)                      27   6.8  
SB_50621| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   6.8  
SB_40568| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   9.0  
SB_39120| Best HMM Match : SH3_1 (HMM E-Value=2e-20)                   27   9.0  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  116 bits (279), Expect = 1e-26
 Identities = 62/119 (52%), Positives = 72/119 (60%), Gaps = 1/119 (0%)
 Frame = +2

Query: 155  KKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWD 334
            K + + FS K + ++       KDD  THLYTLIV+PDNTYEV IDNEKVESG+LE DWD
Sbjct: 825  KTLVIQFSVKHEQNIDCGGGYAKDDEMTHLYTLIVRPDNTYEVKIDNEKVESGELEKDWD 884

Query: 335  FLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXHIPDP-MHQPETG**XDEMD 508
            FLPPK IKDPEAKKPEDW                     +IPDP   +PE     DE+D
Sbjct: 885  FLPPKTIKDPEAKKPEDWDDKKMIDDPEDKKPEDWDKPQNIPDPDAKKPEDW--DDEID 941



 Score = 51.6 bits (118), Expect = 4e-07
 Identities = 41/129 (31%), Positives = 62/129 (48%)
 Frame = +2

Query: 2    LVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSY 181
            LV+QF+VKHEQ+IDCGGGY K      + +  H    Y ++  PD     T +V +    
Sbjct: 827  LVIQFSVKHEQNIDCGGGYAK------DDEMTH---LYTLIVRPD----NTYEVKI---- 869

Query: 182  KGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKD 361
               N  ++     KD  +    T+        E   D + ++  + +   D+  P+ I D
Sbjct: 870  --DNEKVESGELEKDWDFLPPKTIKDPEAKKPEDWDDKKMIDDPEDKKPEDWDKPQNIPD 927

Query: 362  PEAKKPEDW 388
            P+AKKPEDW
Sbjct: 928  PDAKKPEDW 936


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score = 93.9 bits (223), Expect = 7e-20
 Identities = 53/117 (45%), Positives = 68/117 (58%), Gaps = 12/117 (10%)
 Frame = +2

Query: 74  CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKKDIRCK--------- 223
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN  I++    K         
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGED-RKLHFIFRHKNPKNGTIEEKHAKKPTGNYNSVF 86

Query: 224 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPEAKKPEDW 388
           D   THL+TL+V+PDNT+EV ID E V  G L  D      PP +I+DP+ K+PEDW
Sbjct: 87  DGKKTHLFTLVVRPDNTFEVFIDQESVNKGSLLEDMTPPVNPPAEIEDPDDKRPEDW 143



 Score = 35.1 bits (77), Expect = 0.034
 Identities = 17/35 (48%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +2

Query: 290 DNEKVESGDLEA--DWDFLPPKKIKDPEAKKPEDW 388
           + EK+   D E   DWD   P KI D  AKKPE W
Sbjct: 145 EREKIPDPDAEKPDDWDEDAPVKIPDTNAKKPEGW 179


>SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)
          Length = 551

 Score = 29.5 bits (63), Expect = 1.7
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +2

Query: 209 DIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWD 334
           D+ CKDD         V  D T E L+D+ +V+S  L  D D
Sbjct: 485 DVSCKDDKNRTALHWAVGQDKTIEGLLDDPRVQSLQLVNDQD 526


>SB_10791| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 510

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = +2

Query: 290 DNEKVESGDLEADWDFLPPKKIKDPEAKKPE 382
           D++  + GD E +  FLPP    + E+K PE
Sbjct: 106 DSDNGDKGDSEDEESFLPPSIFDESESKGPE 136


>SB_58170| Best HMM Match : Spindle_assoc (HMM E-Value=2.3)
          Length = 310

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +3

Query: 351 KSRTLKPRNQKTGMTSPLFQTPKTRSLRIGTSLNTFQIQCT 473
           K R LKP+  K+ + S      KT +L++  SL+ +  QC+
Sbjct: 28  KLRGLKPKTHKSEVGSIANARNKTETLKLDRSLSNYDKQCS 68


>SB_43952| Best HMM Match : Spindle_assoc (HMM E-Value=2.3)
          Length = 310

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +3

Query: 351 KSRTLKPRNQKTGMTSPLFQTPKTRSLRIGTSLNTFQIQCT 473
           K R LKP+  K+ + S      KT +L++  SL+ +  QC+
Sbjct: 28  KLRGLKPKTHKSEVGSIANARNKTETLKLDRSLSNYDKQCS 68


>SB_27442| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 165

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
 Frame = +2

Query: 296 EKVESGDLEADWD-FLPPKKIKDPEAKKPEDW 388
           EKV +G++  ++D  LPP++ K PE+  PE +
Sbjct: 48  EKVWTGEVWREFDPLLPPRRQKGPESLDPETY 79


>SB_5367| Best HMM Match : wnt (HMM E-Value=0)
          Length = 367

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 9/33 (27%), Positives = 17/33 (51%)
 Frame = -3

Query: 314 RQIQLSHCQ*GLHRCCQVSQSECTNVCRHHLCS 216
           +++Q + C+   H CC+V    C      H+C+
Sbjct: 335 KEVQATRCRCKFHWCCKVKCKTCIKNVTTHICN 367


>SB_45399| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 573

 Score = 28.3 bits (60), Expect = 3.9
 Identities = 22/97 (22%), Positives = 35/97 (36%), Gaps = 2/97 (2%)
 Frame = -1

Query: 298 LIVNEDFIGVVRFHNQSVQMCVDIIFAADIFFDEMVLTL--VTEDYVYLLGSRTTNVRAE 125
           + + E F   V  HN S Q   DI+   D+      LTL       V  L  + +  +  
Sbjct: 50  IFLGETFASYVSVHNDSNQSVKDIVIKTDLQTSSQRLTLSGAANMPVAKLDPQKSYDQVI 109

Query: 124 HNLIWSLSVHVLLLQFAVKDLEVSASTVNVLFMFDSE 14
           H+ +  L  H+L+   +   L          F F  +
Sbjct: 110 HHEVKELGTHILVCAVSYSSLAGEKMYFRKFFKFQDD 146


>SB_5714| Best HMM Match : CBM_X (HMM E-Value=1.9)
          Length = 975

 Score = 27.5 bits (58), Expect = 6.8
 Identities = 10/15 (66%), Positives = 11/15 (73%)
 Frame = -3

Query: 278 HRCCQVSQSECTNVC 234
           HRCC+ S S  TNVC
Sbjct: 138 HRCCETSPSWKTNVC 152


>SB_50621| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 264

 Score = 27.5 bits (58), Expect = 6.8
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +3

Query: 342 RLRKSRTLKPRNQKTGMTSPLFQTPKTRSL 431
           R  + +T+K   QKT ++SP +  P T+ L
Sbjct: 88  RYAEQKTVKKEEQKTTISSPCYDFPLTKKL 117


>SB_40568| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 240

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 15/46 (32%), Positives = 25/46 (54%)
 Frame = +3

Query: 279 KSSLTMRKLNLAT*RQTGTSFRLRKSRTLKPRNQKTGMTSPLFQTP 416
           +S  T  +L +A   + G SF LRK  +  PR+++  + S  F+ P
Sbjct: 98  RSKSTDYQLEVAKGGERGKSFNLRKFFSKDPRSREPRVPSTCFRRP 143


>SB_39120| Best HMM Match : SH3_1 (HMM E-Value=2e-20)
          Length = 524

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
 Frame = +3

Query: 264 LTTPMKSSLTMRKLNLAT*RQTGT-SFRLRKSRTLKPRNQKTGMTSPLFQTPKTRSLRIG 440
           L + +++  T R ++    R  G  SF+     T+  +  +    +PLFQTP  ++L+  
Sbjct: 140 LDSVLETPGTARLMDTGMLRTPGNASFQTPGVNTMYQQQIQRTPETPLFQTPPKQTLQAH 199

Query: 441 TSLNTFQ 461
           T   TFQ
Sbjct: 200 TGRVTFQ 206


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,904,300
Number of Sequences: 59808
Number of extensions: 379315
Number of successful extensions: 974
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 969
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1123894172
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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