Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS308A06f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g10840.2 68414.m01245 eukaryotic translation initiation facto... 100 5e-22
At1g10840.1 68414.m01246 eukaryotic translation initiation facto... 100 5e-22
At5g66930.2 68418.m08437 expressed protein similar to unknown pr... 30 1.1
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 30 1.1
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t... 29 1.4
At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low sim... 29 2.5
At5g44960.1 68418.m05514 F-box family protein contains F-box dom... 28 3.3
At4g02750.1 68417.m00375 pentatricopeptide (PPR) repeat-containi... 28 3.3
At1g61660.2 68414.m06950 basic helix-loop-helix (bHLH) family pr... 28 4.4
At1g61660.1 68414.m06949 basic helix-loop-helix (bHLH) family pr... 28 4.4
At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR016... 27 5.8
At4g02970.1 68417.m00404 ubiquitin family protein contains INTER... 27 7.7
>At1g10840.2 68414.m01245 eukaryotic translation initiation factor 3
subunit 3 / eIF-3 gamma / eIF3h (TIF3H1) identical to
SP|Q9C5Z2 Eukaryotic translation initiation factor 3
subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 250
Score = 100 bits (240), Expect = 5e-22
Identities = 54/152 (35%), Positives = 82/152 (53%), Gaps = 2/152 (1%)
Frame = -1
Query: 521 EVPIVIRNSPLTNIMISELTEMIPEEEGS-KFLDLGTASVLEGQLRSLMERVDELNQEAI 345
E+PI + NS L + ++EL P +G L T LE + L++ +D+L+ E
Sbjct: 98 EIPIKVSNSALVSAFMTELETDTPVSQGDYDRLHSSTTPFLENNMEFLIKCMDDLSMEQQ 157
Query: 344 KFNRYQQLVVRQQQDKHRWMVKRAQENAARAAKDETPLPEED-VNKLFKPHPVPPRLNPM 168
KF Y + + RQQ + W+ KR EN AR + E PLPEED N +FK P P RL
Sbjct: 158 KFQYYYRNLSRQQAQQQAWLQKRRTENMARKSAGEEPLPEEDPSNPIFKAIPEPSRLESF 217
Query: 167 IVAGQIDTYSQHISQFCSQSLAKLYLTQALQN 72
++ Q+ + I+ Q+ ++LYLT+AL +
Sbjct: 218 LITNQVSNFCGQINGVAGQNFSRLYLTKALHD 249
>At1g10840.1 68414.m01246 eukaryotic translation initiation factor 3
subunit 3 / eIF-3 gamma / eIF3h (TIF3H1) identical to
SP|Q9C5Z2 Eukaryotic translation initiation factor 3
subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 337
Score = 100 bits (240), Expect = 5e-22
Identities = 54/152 (35%), Positives = 82/152 (53%), Gaps = 2/152 (1%)
Frame = -1
Query: 521 EVPIVIRNSPLTNIMISELTEMIPEEEGS-KFLDLGTASVLEGQLRSLMERVDELNQEAI 345
E+PI + NS L + ++EL P +G L T LE + L++ +D+L+ E
Sbjct: 185 EIPIKVSNSALVSAFMTELETDTPVSQGDYDRLHSSTTPFLENNMEFLIKCMDDLSMEQQ 244
Query: 344 KFNRYQQLVVRQQQDKHRWMVKRAQENAARAAKDETPLPEED-VNKLFKPHPVPPRLNPM 168
KF Y + + RQQ + W+ KR EN AR + E PLPEED N +FK P P RL
Sbjct: 245 KFQYYYRNLSRQQAQQQAWLQKRRTENMARKSAGEEPLPEEDPSNPIFKAIPEPSRLESF 304
Query: 167 IVAGQIDTYSQHISQFCSQSLAKLYLTQALQN 72
++ Q+ + I+ Q+ ++LYLT+AL +
Sbjct: 305 LITNQVSNFCGQINGVAGQNFSRLYLTKALHD 336
>At5g66930.2 68418.m08437 expressed protein similar to unknown
protein (pir||T38383)
Length = 215
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = -3
Query: 144 IQPTYKPVL*SESSEIVFDSGSSERERSETKQLV 43
+QPT PV S S++V D G + ERS + L+
Sbjct: 118 LQPTKPPVGKSHHSKLVMDPGEASEERSSRRTLL 151
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 29.9 bits (64), Expect = 1.1
Identities = 15/58 (25%), Positives = 32/58 (55%)
Frame = -1
Query: 356 QEAIKFNRYQQLVVRQQQDKHRWMVKRAQENAARAAKDETPLPEEDVNKLFKPHPVPP 183
+E ++ R ++ +R+++++ KR +E A RA ++ EE+ + + P P PP
Sbjct: 652 REDVERKRREEEAMRREEER-----KREEEAAKRAEEERRKKEEEEEKRRWPPQPKPP 704
>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
AMP-binding protein GB:CAA96521 from [Brassica napus]
(Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-CoA synthetase-like protein GI:20799732
Length = 722
Score = 29.5 bits (63), Expect = 1.4
Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 8/92 (8%)
Frame = -1
Query: 452 PEEEGSKFLDLGTASVLEGQLRSLM-----ERVD--ELNQEAIKFNRYQQLVVRQQQDKH 294
P+ + G VLEG+ + + E V+ E+ + A++ N QQ+VV Q +
Sbjct: 568 PQHSTGRSRSCGGVIVLEGRAKDTIVLSTGENVEPLEIEEAAMRSNLIQQIVVIGQDQRR 627
Query: 293 RWMVKRAQENAAR-AAKDETPLPEEDVNKLFK 201
+ + AA AAK + + +VN+L K
Sbjct: 628 LGAIVIPNKEAAEGAAKQKISPVDSEVNELSK 659
>At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low
similarity to breast cancer susceptibility protein
[Gallus gallus] GI:19568157; contains Pfam profile
PF00634: BRCA2 repeat
Length = 1155
Score = 28.7 bits (61), Expect = 2.5
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +2
Query: 11 MTKSFLIPYKKTSCFVSLLSRSEEPESNTISLDSDYRTGLYVGCMCLFVR 160
+T+ + I YK+ + RSE ES I L + R+ L G MC + R
Sbjct: 777 ITRIYPILYKERLGEKKSIVRSERIESRIIQLHNQRRSALVEGIMCEYQR 826
>At5g44960.1 68418.m05514 F-box family protein contains F-box domain
Pfam:PF00646
Length = 416
Score = 28.3 bits (60), Expect = 3.3
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 5/43 (11%)
Frame = +3
Query: 342 LDRFLIQFINTFHETAKLSF-----ENRCSTQIQKLGSFLFRY 455
LD F +QF N HE + F E+ C +++QK F+ RY
Sbjct: 47 LDLFSLQFTNPHHEEGLIKFMDRFMESNCRSRLQK---FMIRY 86
>At4g02750.1 68417.m00375 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 781
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -2
Query: 403 SKDNFA-VSWNVLMN*IKKRSSSIGTNSWWSGSNKTSIVGW 284
S++N+A VSWN L+ K+ + ++ N +V W
Sbjct: 213 SRENWALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDVVSW 253
>At1g61660.2 68414.m06950 basic helix-loop-helix (bHLH) family
protein contains Pfam domain, PF00010: Helix-loop-helix
DNA-binding domain
Length = 347
Score = 27.9 bits (59), Expect = 4.4
Identities = 21/88 (23%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = -1
Query: 326 QLVVRQQQDKHRWMVKRAQENAARAAKDETPLPEEDVNKLFKPHPVPP-RLNPMIVAGQI 150
Q++ + +DK + + RAQ + + AKD ++ ++ P P+P ++ + QI
Sbjct: 233 QIISTRLEDKTKNLKTRAQSESLKRAKDNESAAKKP--RVTTPSPLPTFKVRKENLRDQI 290
Query: 149 DTYSQHISQFCSQSLAKLYLTQALQNAK 66
+ Q +S F A + L +A++ K
Sbjct: 291 TSLQQLVSPFGKTDTASV-LQEAIEYIK 317
>At1g61660.1 68414.m06949 basic helix-loop-helix (bHLH) family
protein contains Pfam domain, PF00010: Helix-loop-helix
DNA-binding domain
Length = 393
Score = 27.9 bits (59), Expect = 4.4
Identities = 21/88 (23%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = -1
Query: 326 QLVVRQQQDKHRWMVKRAQENAARAAKDETPLPEEDVNKLFKPHPVPP-RLNPMIVAGQI 150
Q++ + +DK + + RAQ + + AKD ++ ++ P P+P ++ + QI
Sbjct: 233 QIISTRLEDKTKNLKTRAQSESLKRAKDNESAAKKP--RVTTPSPLPTFKVRKENLRDQI 290
Query: 149 DTYSQHISQFCSQSLAKLYLTQALQNAK 66
+ Q +S F A + L +A++ K
Sbjct: 291 TSLQQLVSPFGKTDTASV-LQEAIEYIK 317
>At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR01612:
reticulocyte binding protein; similar to Myosin heavy
chain, non-muscle (Zipper protein) (Myosin II)
(SP:Q99323) {Drosophila melanogaster} similar to EST
gb|T76116
Length = 1730
Score = 27.5 bits (58), Expect = 5.8
Identities = 21/64 (32%), Positives = 35/64 (54%)
Frame = -1
Query: 506 IRNSPLTNIMISELTEMIPEEEGSKFLDLGTASVLEGQLRSLMERVDELNQEAIKFNRYQ 327
+RNS + NI + L+ + EEG F ++E ++RSL E EL + ++NR +
Sbjct: 463 VRNSIIENIE-TILSNIYTPEEGHSF------DIVE-KVRSLAEERKELTNVSQEYNRLK 514
Query: 326 QLVV 315
L+V
Sbjct: 515 DLIV 518
>At4g02970.1 68417.m00404 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 270
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = -3
Query: 159 RTNRHIQPTYKPVL*SESSEIVFDSGSSERERSETKQLVFLYGIK 25
RTN+ +QPT + + E++ DS S ++ +T + F +K
Sbjct: 146 RTNQVVQPTEQASTSGTAKEVLRDSDSPVEKKIKTNPMKFTVHVK 190
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,974,040
Number of Sequences: 28952
Number of extensions: 225414
Number of successful extensions: 753
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 737
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 751
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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