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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS307G04f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g06160.1 68418.m00687 splicing factor-related contains some s...   119   1e-27
At5g16780.1 68418.m01965 SART-1 family protein contains Pfam dom...    31   0.36 
At4g00380.1 68417.m00052 XH/XS domain-containing protein / XS zi...    30   1.1  
At3g12550.1 68416.m01562 XH/XS domain-containing protein / XS zi...    29   1.4  
At2g47250.1 68415.m05900 RNA helicase, putative similar to SP|P5...    29   1.4  
At1g44910.1 68414.m05146 FF domain-containing protein / WW domai...    29   1.4  
At3g62310.1 68416.m07000 RNA helicase, putative similar to SP|P5...    29   2.5  
At2g27170.1 68415.m06029 structural maintenance of chromosomes (...    29   2.5  
At1g65390.2 68414.m07419 disease resistance protein (TIR class),...    29   2.5  
At1g65390.1 68414.m07420 disease resistance protein (TIR class),...    29   2.5  
At4g36050.2 68417.m05132 endonuclease/exonuclease/phosphatase fa...    28   3.3  
At4g36050.1 68417.m05131 endonuclease/exonuclease/phosphatase fa...    28   3.3  
At1g15910.1 68414.m01908 XH/XS domain-containing protein / XS zi...    28   3.3  
At3g53030.1 68416.m05845 protein kinase family protein contains ...    27   5.8  
At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative ...    27   5.8  

>At5g06160.1 68418.m00687 splicing factor-related contains some
           similarity to splicing factor SP:Q12874 from [Homo
           sapiens]
          Length = 504

 Score =  119 bits (286), Expect = 1e-27
 Identities = 64/169 (37%), Positives = 102/169 (60%), Gaps = 5/169 (2%)
 Frame = +2

Query: 20  ILEQQRSYHEERERTMDAMVKEILHKKTGHRETINADHRLKNLHDRYIEASIRLKELYED 199
           +LEQ RS HEE ER    +V+++  +    ++ +   HR++++ +  +  + +L E YED
Sbjct: 5   LLEQTRSNHEEVERLERLVVEDLQKEPPSSKDRLVQGHRVRHMIESIMLTTEKLVETYED 64

Query: 200 KDGLRKEEISAL-----SGPHEFQEFYSRLKQIKEFHRKHPNEISVPMSVEFEELANQRE 364
           KDG   +EI+AL     +G + F EFY RLK+I+E+H++HP+   V  + ++E  A  +E
Sbjct: 65  KDGAWDDEIAALGGQTATGTNVFSEFYDRLKEIREYHKRHPSGRLVDANEDYE--ARLKE 122

Query: 365 NPAEDYTVPVEFTDEEGYGKYLDLHSCYEKYINLKGIEKVDYITYLSIF 511
            P       + F+ EEG G+YLDLH  Y +YIN K  E+V+Y  YL +F
Sbjct: 123 EPI------IAFSGEEGNGRYLDLHDMYNQYINSKFGERVEYSAYLDVF 165


>At5g16780.1 68418.m01965 SART-1 family protein contains Pfam
           domain, PF03343: SART-1 family
          Length = 820

 Score = 31.5 bits (68), Expect = 0.36
 Identities = 21/69 (30%), Positives = 31/69 (44%)
 Frame = +2

Query: 23  LEQQRSYHEERERTMDAMVKEILHKKTGHRETINADHRLKNLHDRYIEASIRLKELYEDK 202
           +E+ +S HE RE   D     +   + G R+    DHR K+    Y    IR K+   DK
Sbjct: 3   VEKSKSRHEIREERADYEGSPVREHRDGRRK--EKDHRSKDKEKDYDREKIRDKDHRRDK 60

Query: 203 DGLRKEEIS 229
           +  R  + S
Sbjct: 61  EKERDRKRS 69


>At4g00380.1 68417.m00052 XH/XS domain-containing protein / XS zinc
           finger domain-containing protein contains Pfam domains
           PF03469: XH domain, PF03468: XS domain and PF03470: XS
           zinc finger domain
          Length = 635

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 28/118 (23%), Positives = 50/118 (42%)
 Frame = +2

Query: 8   RHEAILEQQRSYHEERERTMDAMVKEILHKKTGHRETINADHRLKNLHDRYIEASIRLKE 187
           +HEA+ E +R   +E +R  DAM K +       ++   +  RL   H R  E ++  K 
Sbjct: 344 KHEALTELERQKLDEDKRKSDAMNKSLQLASREQKKADESVLRLVEEHQRQKEDALN-KI 402

Query: 188 LYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEFHRKHPNEISVPMSVEFEELANQR 361
           L  +K    K+ +       E QE   +L+ +K         +   M    +EL +++
Sbjct: 403 LLLEKQLDTKQTLEM-----EIQELKGKLQVMKHLGDDDDEAVQTKMKEMNDELDDKK 455


>At3g12550.1 68416.m01562 XH/XS domain-containing protein / XS zinc
           finger domain-containing protein contains Pfam domains
           PF03469: XH domain, PF03468: XS domain and PF03470: XS
           zinc finger domain
          Length = 635

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 24/107 (22%), Positives = 45/107 (42%), Gaps = 2/107 (1%)
 Frame = +2

Query: 122 NADHRLKNLHDRYIEASIRLKELYEDKDGLRKEEISALSG-PHEFQEFYSRLKQIKEFHR 298
           N  +RLK L  +Y + S++LK   E+K+ + +     L+G   +  + ++R+    E  +
Sbjct: 270 NKQYRLKKLQIKYSQDSVKLKYETEEKEKILRAYSEDLTGRQQKSTDHFNRIFADHEKQK 329

Query: 299 KHPNEISVPMSVEFEELA-NQRENPAEDYTVPVEFTDEEGYGKYLDL 436
                    + +   ELA  + EN  +   V  E         Y+ L
Sbjct: 330 VQLESQIKELEIRKLELAKREAENETQRKIVAKELEQNAAINSYVQL 376


>At2g47250.1 68415.m05900 RNA helicase, putative similar to
           SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
           (Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
           profiles PF04408: Helicase associated domain (HA2),
           PF00271: Helicase conserved C-terminal domain
          Length = 729

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
 Frame = +2

Query: 137 LKNLHDRYIEASIR---LKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEFHRKHP 307
           L   H+R +   +    LKE+  ++  L+   +SA     +FQE++S    +K   R HP
Sbjct: 190 LDEAHERTLATDVLFGLLKEVLRNRPDLKLVVMSATLEAEKFQEYFSGAPLMKVPGRLHP 249

Query: 308 NEI 316
            EI
Sbjct: 250 VEI 252


>At1g44910.1 68414.m05146 FF domain-containing protein / WW
           domain-containing protein contains Pfam profiles
           PF01846: FF domain, PF00397: WW domain
          Length = 946

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 26/90 (28%), Positives = 39/90 (43%), Gaps = 2/90 (2%)
 Frame = +2

Query: 26  EQQRSYHEERERTMDAMVKEILHKKTGHRETINADHRLKNLHDRYIEASIRLKELYEDKD 205
           E ++ YHE++    DAM      +K   +  I+ D   + + D      I LK +Y+D  
Sbjct: 671 ELEKQYHEDKSYVKDAMKS----RKANFKSAISEDLSTQQISD------INLKLIYDDLV 720

Query: 206 G--LRKEEISALSGPHEFQEFYSRLKQIKE 289
           G    KEE  A       +EF + L   KE
Sbjct: 721 GRVKEKEEKEARKLQRLAEEFTNLLHTFKE 750


>At3g62310.1 68416.m07000 RNA helicase, putative similar to
           SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
           (Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
           profiles PF04408: Helicase associated domain (HA2),
           PF00271: Helicase conserved C-terminal domain
          Length = 726

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 18/63 (28%), Positives = 32/63 (50%), Gaps = 3/63 (4%)
 Frame = +2

Query: 137 LKNLHDRYIEASIR---LKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEFHRKHP 307
           L   H+R +   +    LKE+ +++  L+   +SA     +FQ+++S    +K   R HP
Sbjct: 186 LDEAHERTLATDVLFGLLKEVLKNRPDLKLVVMSATLEAEKFQDYFSGAPLMKVPGRLHP 245

Query: 308 NEI 316
            EI
Sbjct: 246 VEI 248


>At2g27170.1 68415.m06029 structural maintenance of chromosomes
           (SMC) family protein similar to basement
           membrane-associated chondroitin proteoglycan Bamacan
           [Rattus norvegicus] GI:1785540; contains Pfam profile
           PF02463: RecF/RecN/SMC N terminal domain. No suitalble
           start codon was identified.
          Length = 1207

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 22/100 (22%), Positives = 50/100 (50%)
 Frame = +2

Query: 65  MDAMVKEILHKKTGHRETINADHRLKNLHDRYIEASIRLKELYEDKDGLRKEEISALSGP 244
           +D  ++E+  +K   R+    D + K+L     E +I  KEL++ ++ L + E++     
Sbjct: 196 LDERLRELDEEKEELRKYQQLDKQRKSL-----EYTIYDKELHDAREKLEQVEVARTKAS 250

Query: 245 HEFQEFYSRLKQIKEFHRKHPNEISVPMSVEFEELANQRE 364
            E  + Y R+++ ++   K  +E    ++ E + L  ++E
Sbjct: 251 EESTKMYDRVEKAQD-DSKSLDESLKELTKELQTLYKEKE 289


>At1g65390.2 68414.m07419 disease resistance protein (TIR class),
           putative domain signature TIR exists, suggestive of a
           disease resistance protein.
          Length = 297

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 2/81 (2%)
 Frame = +2

Query: 179 LKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEFHRKHPNEISVPMSV--EFEELA 352
           +K + +    ++K      +G  E+Q+F     ++     + PNE +V ++V  EF ++ 
Sbjct: 176 VKSIVKAVKKVQKNFFQRRNGEIEYQDFSVPACKLTITMHESPNEEAVQVTVLNEFYQMK 235

Query: 353 NQRENPAEDYTVPVEFTDEEG 415
           NQ   P+ ++   V+ T  +G
Sbjct: 236 NQSPVPSYEFKFWVDLTRPKG 256


>At1g65390.1 68414.m07420 disease resistance protein (TIR class),
           putative domain signature TIR exists, suggestive of a
           disease resistance protein.
          Length = 411

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 2/81 (2%)
 Frame = +2

Query: 179 LKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEFHRKHPNEISVPMSV--EFEELA 352
           +K + +    ++K      +G  E+Q+F     ++     + PNE +V ++V  EF ++ 
Sbjct: 176 VKSIVKAVKKVQKNFFQRRNGEIEYQDFSVPACKLTITMHESPNEEAVQVTVLNEFYQMK 235

Query: 353 NQRENPAEDYTVPVEFTDEEG 415
           NQ   P+ ++   V+ T  +G
Sbjct: 236 NQSPVPSYEFKFWVDLTRPKG 256


>At4g36050.2 68417.m05132 endonuclease/exonuclease/phosphatase
           family protein
          Length = 609

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 22/100 (22%), Positives = 41/100 (41%)
 Frame = +2

Query: 113 ETINADHRLKNLHDRYIEASIRLKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEF 292
           ET+ A   + NL D    +S+R   +   +DG RK+     S     + F++   ++   
Sbjct: 428 ETVAATGSIDNLSDGIRASSVRALNI--SRDGDRKKARKIQSSQLSLKSFFTTNSKVNNV 485

Query: 293 HRKHPNEISVPMSVEFEELANQRENPAEDYTVPVEFTDEE 412
                + +S   S + E +     +  ED + P   T E+
Sbjct: 486 EDSSSSYVSSSPSSQVESITEPNVSGKED-SEPTTSTQEQ 524


>At4g36050.1 68417.m05131 endonuclease/exonuclease/phosphatase
           family protein
          Length = 408

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 22/100 (22%), Positives = 41/100 (41%)
 Frame = +2

Query: 113 ETINADHRLKNLHDRYIEASIRLKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEF 292
           ET+ A   + NL D    +S+R   +   +DG RK+     S     + F++   ++   
Sbjct: 227 ETVAATGSIDNLSDGIRASSVRALNI--SRDGDRKKARKIQSSQLSLKSFFTTNSKVNNV 284

Query: 293 HRKHPNEISVPMSVEFEELANQRENPAEDYTVPVEFTDEE 412
                + +S   S + E +     +  ED + P   T E+
Sbjct: 285 EDSSSSYVSSSPSSQVESITEPNVSGKED-SEPTTSTQEQ 323


>At1g15910.1 68414.m01908 XH/XS domain-containing protein / XS zinc
           finger domain-containing protein contains Pfam domains
           PF03469: XH domain, PF03468: XS domain and PF03470: XS
           zinc finger domain
          Length = 634

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 28/118 (23%), Positives = 49/118 (41%)
 Frame = +2

Query: 8   RHEAILEQQRSYHEERERTMDAMVKEILHKKTGHRETINADHRLKNLHDRYIEASIRLKE 187
           +HEA+ E  R   +E +R  DAM K +       ++   +  RL   H R  E ++  K 
Sbjct: 343 KHEALTELDRQKLDEDKRKSDAMNKSLQLASREQKKADESVLRLVEEHQRQKEDALN-KI 401

Query: 188 LYEDKDGLRKEEISALSGPHEFQEFYSRLKQIKEFHRKHPNEISVPMSVEFEELANQR 361
           L  +K    K+ +       E QE   +L+ +K         +   M    +EL +++
Sbjct: 402 LLLEKQLDTKQTLEM-----EIQELKGKLQVMKHLGDDDDEAVQKKMKEMNDELDDKK 454


>At3g53030.1 68416.m05845 protein kinase family protein contains
           eukaryotic protein kinase domain, INTERPRO:IPR000719
          Length = 529

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +1

Query: 52  KGKNHGRNGQRNSSQKNWASGDYKC 126
           KGK  G+ G R+S +   AS D KC
Sbjct: 282 KGKQGGKKGSRSSRRHLVASADLKC 306


>At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative
           similar to RNA-directed RNA polymerase GB:CAA09697
           GI:4138282 [Nicotiana tabacum]
          Length = 1107

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 12/55 (21%), Positives = 27/55 (49%)
 Frame = +2

Query: 119 INADHRLKNLHDRYIEASIRLKELYEDKDGLRKEEISALSGPHEFQEFYSRLKQI 283
           +N  +R+   + +YI   +R+  + ED + +R  ++S  S      + Y R+  +
Sbjct: 384 VNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDLSPRSSTQRRTKLYDRIYSV 438


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,989,513
Number of Sequences: 28952
Number of extensions: 220532
Number of successful extensions: 731
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 727
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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