Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS307G03f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A... 166 9e-42
At3g25800.1 68416.m03211 serine/threonine protein phosphatase 2A... 165 2e-41
At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A... 162 1e-40
At1g27920.1 68414.m03421 microtubule associated protein (MAP65/A... 29 1.9
At4g29380.1 68417.m04197 protein kinase family protein / WD-40 r... 28 4.4
At3g56650.1 68416.m06299 thylakoid lumenal 20 kDa protein SP:Q9L... 28 4.4
At1g64790.1 68414.m07346 translational activator family protein ... 27 5.8
At3g05910.1 68416.m00666 pectinacetylesterase, putative similar ... 27 7.7
>At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit A (RCN1) identical to
phosphoprotein phosphatase 2A, regulatory subunit A
GI:1262171 from [Arabidopsis thaliana]
Length = 588
Score = 166 bits (403), Expect = 9e-42
Identities = 85/125 (68%), Positives = 97/125 (77%)
Frame = +1
Query: 145 DESLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDEDEVL 324
DE LYPIAVLIDELKN+D+QLRLNSI++LSTIA ALG ERT+ ELIPFL+E D+DEVL
Sbjct: 5 DEPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLSENSDDDDEVL 64
Query: 325 LALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQALEE 504
LA+AE+LG FI VGG EFAH LLPPLE+L VEET VR+KAV SL + L E
Sbjct: 65 LAMAEELGVFIPFVGGIEFAHVLLPPLESLCTVEETCVREKAVESLCKIGSQMKENDLVE 124
Query: 505 HFVPL 519
FVPL
Sbjct: 125 SFVPL 129
Score = 46.0 bits (104), Expect = 2e-05
Identities = 36/126 (28%), Positives = 58/126 (46%), Gaps = 1/126 (0%)
Frame = +1
Query: 142 TDESLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDED-E 318
T E L PI + + LK+E +RLN I KL + +G++ L+P + E D
Sbjct: 355 TIEHLLPIFLSL--LKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVELAEDRHWR 412
Query: 319 VLLALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQAL 498
V LA+ E + + +G G F L + +R+ A +L+ +AE P+
Sbjct: 413 VRLAIIEYVPLLASQLGIGFFDDKLGALCMQWLQDKVYSIREAAANNLKRLAEEFGPEWA 472
Query: 499 EEHFVP 516
+H VP
Sbjct: 473 MQHLVP 478
Score = 35.9 bits (79), Expect = 0.017
Identities = 25/120 (20%), Positives = 58/120 (48%), Gaps = 1/120 (0%)
Frame = +1
Query: 163 IAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYD-EDEVLLALAE 339
+ V+++ +++ ++R +L + A+G + T+++L+P + D E EV +A A
Sbjct: 243 LPVIVNFSQDKSWRVRYMVANQLYELCEAVGPDCTRTDLVPAYVRLLRDNEAEVRIAAAG 302
Query: 340 QLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQALEEHFVPL 519
++ F L+ +LP ++ L++ VR + + +A + EH +P+
Sbjct: 303 KVTKFCRLLNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPILGKDSTIEHLLPI 362
>At3g25800.1 68416.m03211 serine/threonine protein phosphatase 2A
(PP2A) 65 KDa regulatory subunit A identical to protein
phosphatase 2A 65 kDa regulatory subunit (pDF1)
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 165 bits (401), Expect = 2e-41
Identities = 84/125 (67%), Positives = 98/125 (78%)
Frame = +1
Query: 145 DESLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDEDEVL 324
DE LYPIAVLIDELKN+D+QLRLNSI++LSTIA ALG ERT+ ELIPFL+E D+DEVL
Sbjct: 5 DEPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLSENNDDDDEVL 64
Query: 325 LALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQALEE 504
LA+AE+LG FI VGG E+AH LLPPLETL+ VEET VR+KAV SL V L +
Sbjct: 65 LAMAEELGVFIPYVGGVEYAHVLLPPLETLSTVEETCVREKAVESLCRVGSQMRESDLVD 124
Query: 505 HFVPL 519
HF+ L
Sbjct: 125 HFISL 129
Score = 48.4 bits (110), Expect = 3e-06
Identities = 37/126 (29%), Positives = 58/126 (46%), Gaps = 1/126 (0%)
Frame = +1
Query: 142 TDESLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDED-E 318
T E L PI + + LK+E +RLN I KL + +G++ L+P + E D
Sbjct: 355 TIEHLLPIFLSL--LKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVELAEDRHWR 412
Query: 319 VLLALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQAL 498
V LA+ E + + +G G F L + +RD A +L+ +AE P+
Sbjct: 413 VRLAIIEYIPLLASQLGVGFFDDKLGALCMQWLQDKVHSIRDAAANNLKRLAEEFGPEWA 472
Query: 499 EEHFVP 516
+H VP
Sbjct: 473 MQHIVP 478
Score = 39.1 bits (87), Expect = 0.002
Identities = 27/120 (22%), Positives = 58/120 (48%), Gaps = 1/120 (0%)
Frame = +1
Query: 163 IAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYD-EDEVLLALAE 339
+ V+++ +++ ++R +L + A+G E T++EL+P + D E EV +A A
Sbjct: 243 LPVIVNFSQDKSWRVRYMVANQLYELCEAVGPEPTRTELVPAYVRLLRDNEAEVRIAAAG 302
Query: 340 QLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQALEEHFVPL 519
++ F ++ +LP ++ L++ VR + + +A A EH +P+
Sbjct: 303 KVTKFCRILNPEIAIQHILPCVKELSSDSSQHVRSALASVIMGMAPVLGKDATIEHLLPI 362
>At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A
(PP2A) 65 kDa regulatory subunit, putative similar to
protein phosphatase 2A 65 kDa regulatory subunit
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 162 bits (394), Expect = 1e-40
Identities = 84/128 (65%), Positives = 97/128 (75%)
Frame = +1
Query: 136 SGTDESLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDED 315
S DE LYPIAVLIDELKN+D+Q RLNSIK+LS IA ALG ERT+ ELIPFL+E D+D
Sbjct: 2 SMVDEPLYPIAVLIDELKNDDIQRRLNSIKRLSIIARALGEERTRKELIPFLSENNDDDD 61
Query: 316 EVLLALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQA 495
EVLLA+AE+LG FI VGG E+A+ LLPPLETL+ VEET VR+KAV SL +
Sbjct: 62 EVLLAMAEELGGFILYVGGVEYAYVLLPPLETLSTVEETCVREKAVDSLCRIGAQMRESD 121
Query: 496 LEEHFVPL 519
L EHF PL
Sbjct: 122 LVEHFTPL 129
Score = 47.2 bits (107), Expect = 7e-06
Identities = 36/126 (28%), Positives = 58/126 (46%), Gaps = 1/126 (0%)
Frame = +1
Query: 142 TDESLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDED-E 318
T E L PI + + LK+E +RLN I KL + +G++ L+P + E D
Sbjct: 355 TIEHLLPIFLSL--LKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVELAEDRHWR 412
Query: 319 VLLALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQAL 498
V LA+ E + + +G G F L + +R+ A +L+ +AE P+
Sbjct: 413 VRLAIIEYIPLLASQLGVGFFDEKLGALCMQWLQDKVHSIREAAANNLKRLAEEFGPEWA 472
Query: 499 EEHFVP 516
+H VP
Sbjct: 473 MQHIVP 478
Score = 37.9 bits (84), Expect = 0.004
Identities = 26/120 (21%), Positives = 58/120 (48%), Gaps = 1/120 (0%)
Frame = +1
Query: 163 IAVLIDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYD-EDEVLLALAE 339
+ V+++ +++ ++R +L + A+G E T+++L+P + D E EV +A A
Sbjct: 243 LPVIVNFSQDKSWRVRYMVANQLYELCEAVGPEPTRTDLVPAYARLLCDNEAEVRIAAAG 302
Query: 340 QLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQALEEHFVPL 519
++ F ++ +LP ++ L++ VR + + +A A EH +P+
Sbjct: 303 KVTKFCRILNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPVLGKDATIEHLLPI 362
>At1g27920.1 68414.m03421 microtubule associated protein
(MAP65/ASE1) family protein low similarity to protein
regulating cytokinesis 1 (PRC1) [Homo sapiens]
GI:2865521; contains Pfam profile PF03999: Microtubule
associated protein (MAP65/ASE1 family)
Length = 592
Score = 29.1 bits (62), Expect = 1.9
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +1
Query: 340 QLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLR 465
++GN NLV GE H ++ LAA++E + R K A+ R
Sbjct: 377 EMGNITNLVDSGEVDH-----VDLLAAMDEKIARAKEEAASR 413
>At4g29380.1 68417.m04197 protein kinase family protein / WD-40
repeat family protein contains Pfam PF00400: WD domain,
G-beta repeat; contains Pfam PF00069: Protein kinase
domain; contains PF02985: HEAT repeat; similar to
adaptor protein (GI:1817584) [Homo sapiens]; similar to
VPS15 protein (GI:6103009) [Pichia pastoris]
Length = 1494
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/51 (23%), Positives = 26/51 (50%)
Frame = +1
Query: 184 LKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDEDEVLLALA 336
L + D QLR +K+ + +G + L+P++ + + D+ E ++ A
Sbjct: 661 LNDRDEQLRSVFFEKIVYVCFFVGQRSVEEYLLPYIDQALSDQTEAVIVNA 711
>At3g56650.1 68416.m06299 thylakoid lumenal 20 kDa protein
SP:Q9LXX5; PMID:11719511; F22K20.16 - Arabidopsis
thaliana, EMBL:AC002291;
Length = 262
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +1
Query: 259 ERTKSELIPFLTETIYDEDEVLLALAEQLGN 351
E+ + L PF+T YD DE+L E++G+
Sbjct: 174 EKVIASLGPFVTGNSYDSDELLKTSIEKIGD 204
>At1g64790.1 68414.m07346 translational activator family protein
similar to HsGCN1 [Homo sapiens] GI:2282576
Length = 2440
Score = 27.5 bits (58), Expect = 5.8
Identities = 32/126 (25%), Positives = 54/126 (42%), Gaps = 6/126 (4%)
Frame = +1
Query: 139 GTDESLYPIAV--LIDELKNEDVQL-RLNSIKKLSTIALALGVERTKSELIPFLTETIYD 309
G E +P V L + LK++ + R + + LS + ALG + ++ L + +
Sbjct: 1461 GMGEDNFPDLVPWLFETLKSDTSNVERYGAAQGLSEVIAALGTDYFENILPDLIRHCSHQ 1520
Query: 310 EDEVLLALAEQLGNFINLVGGGEFAHCL---LPPLETLAAVEETVVRDKAVASLRAVAEH 480
+ V L F+ G +F L LP + A E VRD A+ + + EH
Sbjct: 1521 KASVRDGYLT-LFKFLPRSLGAQFQKYLQLVLPAILDGLADENESVRDAALGAGHVLVEH 1579
Query: 481 HSPQAL 498
H+ +L
Sbjct: 1580 HATTSL 1585
>At3g05910.1 68416.m00666 pectinacetylesterase, putative similar to
pectinacetylesterase precursor GI:1431629 from [Vigna
radiata]
Length = 415
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +1
Query: 328 ALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVA 456
+++ Q G FIN FAHC +T A + V+R KAVA
Sbjct: 345 SMSRQNGLFINSC----FAHCQTERQDTWFADDSPVIRKKAVA 383
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,355,475
Number of Sequences: 28952
Number of extensions: 159024
Number of successful extensions: 389
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 388
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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