Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS307E12f
(521 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q5MGQ2 Cluster: Cuticle protein 3; n=2; Bombycoidea|Rep... 262 3e-69
UniRef50_Q7PK07 Cluster: ENSANGP00000023169; n=1; Anopheles gamb... 46 5e-04
UniRef50_Q7JR69 Cluster: LP08773p; n=3; Sophophora|Rep: LP08773p... 46 7e-04
UniRef50_Q17G26 Cluster: Putative uncharacterized protein; n=1; ... 43 0.005
UniRef50_Q4V5F7 Cluster: IP07649p; n=4; Sophophora|Rep: IP07649p... 42 0.011
UniRef50_A7BRB9 Cluster: Protein containing RHS repeats; n=1; Be... 38 0.11
UniRef50_Q290D6 Cluster: GA15599-PA; n=1; Drosophila pseudoobscu... 38 0.14
UniRef50_A1Z8Y3 Cluster: CG30042-PA; n=4; Diptera|Rep: CG30042-P... 38 0.14
UniRef50_Q8IBV4 Cluster: Putative uncharacterized protein MAL7P1... 38 0.18
UniRef50_UPI0000D56366 Cluster: PREDICTED: similar to CG30042-PA... 37 0.24
UniRef50_Q9VRM2 Cluster: CG10625-PB, isoform B; n=5; Fungi/Metaz... 34 1.7
UniRef50_Q9VH46 Cluster: CG12807-PA; n=2; Drosophila melanogaste... 34 1.7
UniRef50_UPI0000D56367 Cluster: PREDICTED: similar to CG30042-PA... 33 3.0
UniRef50_Q8ISI8 Cluster: RNA-binding protein Puf1; n=6; Plasmodi... 33 3.0
UniRef50_A4BT67 Cluster: Putative uncharacterized protein; n=1; ... 33 4.0
UniRef50_Q0BWW7 Cluster: Hydrolase, alpha/beta fold family; n=1;... 33 5.2
UniRef50_Q553N1 Cluster: Putative uncharacterized protein; n=2; ... 33 5.2
UniRef50_A7RPX9 Cluster: Predicted protein; n=1; Nematostella ve... 33 5.2
UniRef50_P15917 Cluster: Lethal factor precursor; n=3; Bacillus ... 33 5.2
UniRef50_UPI0000E4729F Cluster: PREDICTED: similar to GA15808-PA... 32 9.2
UniRef50_Q1D010 Cluster: Putative uncharacterized protein; n=1; ... 32 9.2
UniRef50_Q93411 Cluster: Putative uncharacterized protein; n=2; ... 32 9.2
UniRef50_A6RS81 Cluster: Predicted protein; n=1; Botryotinia fuc... 32 9.2
>UniRef50_Q5MGQ2 Cluster: Cuticle protein 3; n=2; Bombycoidea|Rep:
Cuticle protein 3 - Lonomia obliqua (Moth)
Length = 197
Score = 262 bits (643), Expect = 3e-69
Identities = 109/128 (85%), Positives = 120/128 (93%)
Frame = +3
Query: 129 MNSFKVQYVIALCIVAAVGVPVEDGKWHPYHYGDSGKYIPTDEGKYIHIPNPYIHIDNPY 308
MNS KVQYVIALC+VA VG+P+EDGKWHPY YGDSG+YIP+DEGKYIHIPNPYIH+DNPY
Sbjct: 1 MNSVKVQYVIALCVVAVVGIPLEDGKWHPYKYGDSGRYIPSDEGKYIHIPNPYIHLDNPY 60
Query: 309 DGGFGPYAHDYEPYVGEATVQDQYRLILTPPKDIPFYKDGYYKNNGIKIIKQKHNYDEDK 488
GGFGPYAHDYEPYVGEA+VQDQYRL+LT PKDIPFY+DGYYKNNGIKII+QKHNY EDK
Sbjct: 61 GGGFGPYAHDYEPYVGEASVQDQYRLVLTDPKDIPFYQDGYYKNNGIKIIRQKHNYKEDK 120
Query: 489 YDFXFXTD 512
YDF F T+
Sbjct: 121 YDFDFETE 128
>UniRef50_Q7PK07 Cluster: ENSANGP00000023169; n=1; Anopheles gambiae
str. PEST|Rep: ENSANGP00000023169 - Anopheles gambiae
str. PEST
Length = 232
Score = 46.0 bits (104), Expect = 5e-04
Identities = 15/36 (41%), Positives = 25/36 (69%)
Frame = +3
Query: 216 YHYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFG 323
Y+ G+ GKY+P ++G+Y+H+ N Y H++ P G G
Sbjct: 72 YNSGNDGKYVPRNDGRYVHVDNRYKHVEGPSGRGAG 107
>UniRef50_Q7JR69 Cluster: LP08773p; n=3; Sophophora|Rep: LP08773p -
Drosophila melanogaster (Fruit fly)
Length = 324
Score = 45.6 bits (103), Expect = 7e-04
Identities = 24/67 (35%), Positives = 32/67 (47%), Gaps = 12/67 (17%)
Frame = +3
Query: 189 PVEDGKWHPYHYGDSGKYIPTDEGKYIHIPNPYIHIDN------------PYDGGFGPYA 332
P +DGK+H + G+Y H+P PY H+ + PYDGG+GPYA
Sbjct: 45 PSDDGKYHGNSQNEGRSGSQESIGRYHHMPIPYRHVSDQRELGKYHHIPYPYDGGYGPYA 104
Query: 333 HDYEPYV 353
PYV
Sbjct: 105 GSNIPYV 111
Score = 37.9 bits (84), Expect = 0.14
Identities = 17/31 (54%), Positives = 17/31 (54%)
Frame = +3
Query: 258 GKYIHIPNPYIHIDNPYDGGFGPYAHDYEPY 350
GKY HIP PY PY G PY HD PY
Sbjct: 87 GKYHHIPYPYDGGYGPYAGSNIPYVHDDRPY 117
>UniRef50_Q17G26 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 336
Score = 42.7 bits (96), Expect = 0.005
Identities = 25/76 (32%), Positives = 38/76 (50%), Gaps = 15/76 (19%)
Frame = +3
Query: 168 IVAAVGVPVEDGKWHPYHYGDS--GKYIPTDEG---------KYIHIPNP----YIHIDN 302
+V + DGK+ YG+ GKY+ T+ G +Y+H + YIH+
Sbjct: 18 LVETLARAAHDGKYDKKKYGNGNHGKYVHTNGGDYVEDLDRYRYVHYDDGDRGRYIHVHI 77
Query: 303 PYDGGFGPYAHDYEPY 350
PYDGG+G Y +EP+
Sbjct: 78 PYDGGYGNYEGGHEPF 93
>UniRef50_Q4V5F7 Cluster: IP07649p; n=4; Sophophora|Rep: IP07649p -
Drosophila melanogaster (Fruit fly)
Length = 237
Score = 41.5 bits (93), Expect = 0.011
Identities = 31/106 (29%), Positives = 51/106 (48%), Gaps = 5/106 (4%)
Frame = +3
Query: 186 VPVEDGKWHPYHYGDSGKYIPTDEGKYIHIPNPYI--HIDN--PYDGGFGPYAHDYEPYV 353
+ + G++ P +G +G Y+P D GKY H P PY + D PYD + + +
Sbjct: 32 ITFKPGRYVPRLHGATGWYVPDDSGKYRHDPRPYDGGYGDRGVPYDSDLSGIFRNAQRQL 91
Query: 354 GEATVQDQYRLILTPPKD-IPFYKDGYYKNNGIKIIKQKHNYDEDK 488
EA VQ+ + P+D + F D + G +II+ + D D+
Sbjct: 92 -EAKVQEAGEGLSLGPRDHLRFMIDFNFNGTGWQIIQFQWVRDGDE 136
>UniRef50_A7BRB9 Cluster: Protein containing RHS repeats; n=1;
Beggiatoa sp. PS|Rep: Protein containing RHS repeats -
Beggiatoa sp. PS
Length = 2242
Score = 38.3 bits (85), Expect = 0.11
Identities = 26/95 (27%), Positives = 39/95 (41%), Gaps = 3/95 (3%)
Frame = +3
Query: 210 HPYHYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPY---AHDYEPYVGEATVQDQY 380
H Y Y G +P + G + N Y D YD G A Y+P G QD Y
Sbjct: 1919 HNYRYDPYGGVVP-ENGNFTDPHNHYTLTDKEYDESMGLVWFGARHYDPETGVWLTQDSY 1977
Query: 381 RLILTPPKDIPFYKDGYYKNNGIKIIKQKHNYDED 485
R ++ P + ++ Y +N I I + + E+
Sbjct: 1978 RGVIDDPMSL--HRSMYVADNPINYIDEYGYFFEE 2010
>UniRef50_Q290D6 Cluster: GA15599-PA; n=1; Drosophila
pseudoobscura|Rep: GA15599-PA - Drosophila pseudoobscura
(Fruit fly)
Length = 264
Score = 37.9 bits (84), Expect = 0.14
Identities = 22/67 (32%), Positives = 31/67 (46%), Gaps = 5/67 (7%)
Frame = +3
Query: 174 AAVGVPVEDGKWHP-----YHYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPYAHD 338
AAV DG++ Y G+ G+Y +G+Y+H+ N Y H D G Y D
Sbjct: 30 AAVRATAGDGRYKGGNDGRYRGGNDGRYSGASDGRYVHVDNKYEHDDR----AGGAYTGD 85
Query: 339 YEPYVGE 359
YVG+
Sbjct: 86 RNRYVGD 92
>UniRef50_A1Z8Y3 Cluster: CG30042-PA; n=4; Diptera|Rep: CG30042-PA -
Drosophila melanogaster (Fruit fly)
Length = 259
Score = 37.9 bits (84), Expect = 0.14
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
Frame = +3
Query: 216 YHYGDSGKYIPTDEGKYIHIPNPYIHIDNP---YDGGFGPYAHD 338
Y G G+Y ++G+Y+H+ N Y H D P Y G G Y D
Sbjct: 47 YRGGGDGRYSGGNDGRYVHMDNKYKHDDRPGGDYSGDAGRYVGD 90
>UniRef50_Q8IBV4 Cluster: Putative uncharacterized protein MAL7P1.68;
n=3; Plasmodium|Rep: Putative uncharacterized protein
MAL7P1.68 - Plasmodium falciparum (isolate 3D7)
Length = 1321
Score = 37.5 bits (83), Expect = 0.18
Identities = 26/90 (28%), Positives = 40/90 (44%), Gaps = 3/90 (3%)
Frame = +3
Query: 252 DEGKYIHIPNPYIHIDNPYDGGFGPYAHDYEPYVGEAT--VQDQYRLILTPPKDIPFYKD 425
DE KYI+ N YI+ +N Y Y +D Y+ + + D+ + I K ++ D
Sbjct: 844 DENKYINDKNKYINDENKYINDENKYINDENKYINDENKYINDENKYINYEEK---YHND 900
Query: 426 -GYYKNNGIKIIKQKHNYDEDKYDFXFXTD 512
Y N+ K I K+ Y DK + D
Sbjct: 901 EEKYHNDKNKYINDKNKYINDKNKYHNDND 930
>UniRef50_UPI0000D56366 Cluster: PREDICTED: similar to CG30042-PA;
n=1; Tribolium castaneum|Rep: PREDICTED: similar to
CG30042-PA - Tribolium castaneum
Length = 210
Score = 37.1 bits (82), Expect = 0.24
Identities = 16/39 (41%), Positives = 27/39 (69%)
Frame = +3
Query: 153 VIALCIVAAVGVPVEDGKWHPYHYGDSGKYIPTDEGKYI 269
V+ + ++ + VEDG++ P +SG+Y+PTDEG+YI
Sbjct: 3 VLVIICAFSLSLAVEDGRYVP---DNSGQYVPTDEGQYI 38
>UniRef50_Q9VRM2 Cluster: CG10625-PB, isoform B; n=5; Fungi/Metazoa
group|Rep: CG10625-PB, isoform B - Drosophila
melanogaster (Fruit fly)
Length = 682
Score = 34.3 bits (75), Expect = 1.7
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 7/49 (14%)
Frame = +3
Query: 198 DGKWHPYHYGDSGKYIPTDEGKYIHIPN-------PYIHIDNPYDGGFG 323
+G Y+ + G+Y D GKY+H+ PY+H+ P +GGFG
Sbjct: 471 NGNGGRYNGINDGRYYHDDSGKYVHVEGPTGPPAPPYVHVVGP-EGGFG 518
Score = 32.3 bits (70), Expect = 6.9
Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Frame = +3
Query: 177 AVGVPVEDGKWHPYHYGDSGKYIPTDEGKYIHIPN-PYIHIDNPYDGGFGPYAHDYEPYV 353
A+ ++GK++ G+ G+Y ++G+Y H + Y+H++ P PY H P
Sbjct: 460 AIPYTPDNGKYN----GNGGRYNGINDGRYYHDDSGKYVHVEGPTGPPAPPYVHVVGPEG 515
Query: 354 G 356
G
Sbjct: 516 G 516
>UniRef50_Q9VH46 Cluster: CG12807-PA; n=2; Drosophila
melanogaster|Rep: CG12807-PA - Drosophila melanogaster
(Fruit fly)
Length = 640
Score = 34.3 bits (75), Expect = 1.7
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +3
Query: 264 YIHIPNPYIHIDNPYDGGFGPYAHDYEP-YVGEA 362
+ H+P+ +H P F P +D+EP Y+GEA
Sbjct: 401 HTHVPHHPLHFPPPVPAAFAPITNDFEPHYIGEA 434
>UniRef50_UPI0000D56367 Cluster: PREDICTED: similar to CG30042-PA;
n=1; Tribolium castaneum|Rep: PREDICTED: similar to
CG30042-PA - Tribolium castaneum
Length = 275
Score = 33.5 bits (73), Expect = 3.0
Identities = 22/70 (31%), Positives = 31/70 (44%)
Frame = +3
Query: 195 EDGKWHPYHYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPYAHDYEPYVGEATVQD 374
+DG +HP DSG Y P D G Y Y+H + + GG G +VG A
Sbjct: 41 DDGSYHP---DDSGAYRPDDSGAYRGGYEKYVHQADKF-GGSGGSGGSGGGFVGGARRVG 96
Query: 375 QYRLILTPPK 404
+ + PP+
Sbjct: 97 TFVVSAQPPR 106
Score = 33.1 bits (72), Expect = 4.0
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 5/62 (8%)
Frame = +3
Query: 186 VPVEDGKWHP-----YHYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPYAHDYEPY 350
+ + DG+++P Y+ GD G Y P D G Y P+ Y GG+ Y H + +
Sbjct: 22 IDINDGRYYPDNSGAYNGGDDGSYHPDDSGAY--RPDD----SGAYRGGYEKYVHQADKF 75
Query: 351 VG 356
G
Sbjct: 76 GG 77
>UniRef50_Q8ISI8 Cluster: RNA-binding protein Puf1; n=6;
Plasmodium|Rep: RNA-binding protein Puf1 - Plasmodium
falciparum
Length = 1894
Score = 33.5 bits (73), Expect = 3.0
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +3
Query: 228 DSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPYAHDYEPYV 353
D G+Y D+ KY++ N Y++ DN Y Y +D YV
Sbjct: 57 DEGEYT-NDDNKYVNDENKYVNDDNKYVNDENKYVNDENKYV 97
>UniRef50_A4BT67 Cluster: Putative uncharacterized protein; n=1;
Nitrococcus mobilis Nb-231|Rep: Putative uncharacterized
protein - Nitrococcus mobilis Nb-231
Length = 351
Score = 33.1 bits (72), Expect = 4.0
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -3
Query: 285 KGWECEYIFLRLWGCICQNRRNGKDAIFHPQQVHPPRLRC 166
KGW ++ F RL +CQ RN + P+Q H PR C
Sbjct: 314 KGWYSDWCFRRLTRFLCQELRNASTST--PRQHHLPRESC 351
>UniRef50_Q0BWW7 Cluster: Hydrolase, alpha/beta fold family; n=1;
Hyphomonas neptunium ATCC 15444|Rep: Hydrolase,
alpha/beta fold family - Hyphomonas neptunium (strain
ATCC 15444)
Length = 315
Score = 32.7 bits (71), Expect = 5.2
Identities = 16/66 (24%), Positives = 30/66 (45%)
Frame = +3
Query: 318 FGPYAHDYEPYVGEATVQDQYRLILTPPKDIPFYKDGYYKNNGIKIIKQKHNYDEDKYDF 497
FGP + P +G + ++D + P GYY+N+G+ ++ + N+ + D
Sbjct: 179 FGPVFSQFVPIIGPSQMKDGISSVFAPAPT----PQGYYQNSGLGLLLRPPNFRANAQDM 234
Query: 498 XFXTDE 515
DE
Sbjct: 235 TRLKDE 240
>UniRef50_Q553N1 Cluster: Putative uncharacterized protein; n=2;
Dictyostelium discoideum|Rep: Putative uncharacterized
protein - Dictyostelium discoideum AX4
Length = 844
Score = 32.7 bits (71), Expect = 5.2
Identities = 24/74 (32%), Positives = 31/74 (41%), Gaps = 2/74 (2%)
Frame = +3
Query: 219 HYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGG-FGPYAHDYEPYVGEATVQDQYRLILT 395
H G+ E K I NPYI+I+ G F P H+ D Y++I
Sbjct: 104 HEDIEGQEKKIKENKTIFCINPYIYINFTKCGKIFSPNFHELLKIATSINKNDNYKIIEN 163
Query: 396 P-PKDIPFYKDGYY 434
IP YKDGY+
Sbjct: 164 HYDLVIPNYKDGYW 177
>UniRef50_A7RPX9 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 765
Score = 32.7 bits (71), Expect = 5.2
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +3
Query: 327 YAHDYEPYVGEATVQDQYRLILTPPKDIPFYKDGYYKNNGIKIIKQKHNYDEDKY 491
Y D +P+V QD RL + P D PF + Y +++G ++ + D+D +
Sbjct: 43 YQQDDDPFVRSMHQQDDDRLSMYQPDDGPFMESMYQQDDG-PFMESMYQQDDDPF 96
>UniRef50_P15917 Cluster: Lethal factor precursor; n=3; Bacillus
anthracis|Rep: Lethal factor precursor - Bacillus
anthracis
Length = 809
Score = 32.7 bits (71), Expect = 5.2
Identities = 15/64 (23%), Positives = 28/64 (43%)
Frame = +3
Query: 234 GKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPYAHDYEPYVGEATVQDQYRLILTPPKDIP 413
G Y+P +H P+ + + N +G + H + Y G ++Q L+ K I
Sbjct: 690 GLYVPESRSILLHGPSKGVELRNDSEGFIHEFGHAVDDYAGYLLDKNQSDLVTNSKKFID 749
Query: 414 FYKD 425
+K+
Sbjct: 750 IFKE 753
>UniRef50_UPI0000E4729F Cluster: PREDICTED: similar to GA15808-PA,
partial; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to GA15808-PA, partial -
Strongylocentrotus purpuratus
Length = 528
Score = 31.9 bits (69), Expect = 9.2
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +3
Query: 300 NPYDGGFGPYAHDYEPYVGEATVQDQYRLILTPPKD 407
N D G+GP H P+ G + + Q L+PP+D
Sbjct: 354 NNQDAGWGPQQHPKSPHAGRRSYEPQRTHPLSPPQD 389
>UniRef50_Q1D010 Cluster: Putative uncharacterized protein; n=1;
Myxococcus xanthus DK 1622|Rep: Putative uncharacterized
protein - Myxococcus xanthus (strain DK 1622)
Length = 2138
Score = 31.9 bits (69), Expect = 9.2
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 5/66 (7%)
Frame = +3
Query: 174 AAVGVPVEDGKWHPY--HYGDSGKYIPTDEGKYIHIPNPYIHIDNPY---DGGFGPYAHD 338
A G +DG+W+PY Y + D+G++ P Y Y DG + PY
Sbjct: 56 APQGYYADDGQWYPYPAGYEQQPQGYYADDGQWYPYPAGYEQQPQGYYAADGQWYPYPAG 115
Query: 339 YEPYVG 356
Y+P G
Sbjct: 116 YDPNQG 121
>UniRef50_Q93411 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 423
Score = 31.9 bits (69), Expect = 9.2
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 5/58 (8%)
Frame = +3
Query: 222 YGDSGKYIPTDEGKYIHIPNP-YIHID----NPYDGGFGPYAHDYEPYVGEATVQDQY 380
YGD+ + P+ + +Y + P Y H + + Y G +GPYA Y PY G + Y
Sbjct: 70 YGDTQQQHPSQQ-QYPYYGYPSYSHYNYQPASAYPGAYGPYAGAYGPYAGASGAYRPY 126
>UniRef50_A6RS81 Cluster: Predicted protein; n=1; Botryotinia
fuckeliana B05.10|Rep: Predicted protein - Botryotinia
fuckeliana B05.10
Length = 1164
Score = 31.9 bits (69), Expect = 9.2
Identities = 16/47 (34%), Positives = 19/47 (40%)
Frame = +3
Query: 189 PVEDGKWHPYHYGDSGKYIPTDEGKYIHIPNPYIHIDNPYDGGFGPY 329
PVE+ W P G+ I DE + N ID D G PY
Sbjct: 221 PVENSVWRPRQVGEQHVKIDMDEEPDAFLQNSQFEIDGGVDCGRSPY 267
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 522,377,988
Number of Sequences: 1657284
Number of extensions: 11209607
Number of successful extensions: 27933
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 26835
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27860
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 32619212418
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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