Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS307E07f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 146 1e-35
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 144 3e-35
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 142 1e-34
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 141 2e-34
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 89 1e-18
At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) fa... 29 1.9
At1g01430.1 68414.m00058 expressed protein similar to hypothetic... 29 2.5
At1g54860.1 68414.m06263 expressed protein 28 3.3
At1g49470.1 68414.m05544 expressed protein contains Pfam profile... 28 3.3
At4g01080.1 68417.m00146 expressed protein 28 4.4
At3g51760.1 68416.m05676 hypothetical protein hypothetical prote... 28 4.4
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 27 5.8
At3g21950.1 68416.m02769 S-adenosyl-L-methionine:carboxyl methyl... 27 5.8
At1g69295.1 68414.m07947 beta-1,3-glucanase-related low similari... 27 7.7
At1g66910.1 68414.m07604 protein kinase, putative similar to rec... 27 7.7
At1g11480.1 68414.m01319 eukaryotic translation initiation facto... 27 7.7
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 146 bits (353), Expect = 1e-35
Identities = 65/119 (54%), Positives = 86/119 (72%)
Frame = +2
Query: 155 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWI 334
PAF VG+ G +IWRI+NFEP+PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 335 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSG 511
G++++QDE+G AA+ TV LD GG AVQ+RE GHES FLSYF+ + LEGG SG
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASG 128
Score = 55.2 bits (127), Expect = 3e-08
Identities = 30/108 (27%), Positives = 50/108 (46%)
Frame = +2
Query: 188 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGA 367
+++W I +++ +GK Y GD Y++L T + + W G+ S Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 368 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSG 511
A L + + G VQ R G E F++ FQ + L+GG SG
Sbjct: 454 AVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQ-HMVVLKGGLSSG 500
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 144 bits (349), Expect = 3e-35
Identities = 65/122 (53%), Positives = 85/122 (69%)
Frame = +2
Query: 155 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWI 334
PAF G+ G +IWRI+NFE +PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 335 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSGX 514
G++++QDE+G AA+ TV LD GG AVQHRE GHES FLSYF+ + LEGG SG
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 515 TT 520
T
Sbjct: 128 KT 129
Score = 63.3 bits (147), Expect = 1e-10
Identities = 32/108 (29%), Positives = 53/108 (49%)
Frame = +2
Query: 188 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGA 367
+++W + P+ ++DIGK Y GD Y++L T + + W G++S ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDT 451
Query: 368 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSG 511
A L + + G VQ R G E F++ FQ P+ L+GG SG
Sbjct: 452 AIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQ-PMVVLKGGLSSG 498
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 142 bits (344), Expect = 1e-34
Identities = 63/119 (52%), Positives = 84/119 (70%)
Frame = +2
Query: 155 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWI 334
PAF G+ AG++IWRI+NF P P+ + IGKF+ GDSYI+L+TT+ L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 335 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSG 511
G++++QDE+G AA+ TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SG
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASG 128
Score = 44.4 bits (100), Expect = 5e-05
Identities = 28/111 (25%), Positives = 47/111 (42%)
Frame = +2
Query: 179 TAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDE 358
T +Q+WR+ + D KFY GD Y + + + I W G++S ++E
Sbjct: 391 TGNLQVWRVNGQAKTLLQAADHSKFYSGDCY-VFQYSYPGEEKEEVLIGTWFGKQSVEEE 449
Query: 359 SGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSG 511
G+A + + + Q R G E F Q+ + + +GG SG
Sbjct: 450 RGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSG 499
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 141 bits (342), Expect = 2e-34
Identities = 61/118 (51%), Positives = 85/118 (72%)
Frame = +2
Query: 158 AFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWIG 337
A G+ +G++IWRI+NF+P+ V Q+ GKF+ GDSYI+L+TT+ +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 338 RESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGNPSG 511
++S+QDE+GA A++TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SG
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASG 128
Score = 46.4 bits (105), Expect = 1e-05
Identities = 35/147 (23%), Positives = 63/147 (42%)
Frame = +2
Query: 56 GSVWARTATKPQEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQIWRIQNFEPIPVAQ 235
G V A + + G + S +++D+ K + + T +Q+WRI E I +
Sbjct: 358 GKVAALLQRQGVNVQG-LVKTSSSSSKDEPKPY-----IDGTGNLQVWRINCEEKILLEA 411
Query: 236 KDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAA 415
+ KFY GD Y IL+ + + + W G++S +++ +A L + +
Sbjct: 412 AEQSKFYSGDCY-ILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVP 470
Query: 416 VQHRETMGHESALFLSYFQTPLTYLEG 496
Q R G E F Q+ +T+ G
Sbjct: 471 AQARINEGKEPIQFFVIMQSFITFKGG 497
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 89.4 bits (212), Expect = 1e-18
Identities = 41/116 (35%), Positives = 68/116 (58%)
Frame = +2
Query: 149 VHPAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHY 328
+ AF VG +G++IW + N + I + + GKF+ G++Y++LRT + +DIHY
Sbjct: 8 IDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIHY 67
Query: 329 WIGRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEG 496
W+G ++ + +S A+ + LD G VQ+RE G E+ FLSYF+ + +EG
Sbjct: 68 WLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEG 123
Score = 34.7 bits (76), Expect = 0.038
Identities = 24/104 (23%), Positives = 45/104 (43%)
Frame = +2
Query: 188 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGA 367
+++WR+ + ++ D K + GD Y++ + + ++ WIG ES Q +
Sbjct: 391 LKVWRVDGDDVSLLSIPDQTKLFTGDCYLV-QYKYTYKERTEHLLYVWIGCESIQQDRAD 449
Query: 368 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGG 499
A + G +V G+E + F FQ+ L +GG
Sbjct: 450 AITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQS-LVVFKGG 492
>At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 691
Score = 29.1 bits (62), Expect = 1.9
Identities = 16/40 (40%), Positives = 19/40 (47%), Gaps = 3/40 (7%)
Frame = +3
Query: 321 STTGSVGSRRKTSQEPPQSSPLAWTTSSGAP---RSSTER 431
S+ GS PP SS LAWT+ +P R TER
Sbjct: 409 SSVSRTGSTTSVPPPPPPSSNLAWTSYQNSPHYQRRRTER 448
>At1g01430.1 68414.m00058 expressed protein similar to hypothetical
protein GB:CAB80917 GI:7267605 from [Arabidopsis
thaliana]
Length = 456
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/45 (31%), Positives = 24/45 (53%), Gaps = 4/45 (8%)
Frame = -2
Query: 259 FVEFSYVLLSNGYWF---EVLYSPYLHTGCSADIGKSRM-DFGFV 137
++ F YV++S G WF + + TGC GK+ M + G++
Sbjct: 254 YINFDYVVISGGKWFLKTTIFHENNTVTGCHYCQGKNNMTELGYL 298
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 204 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 103
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
>At1g49470.1 68414.m05544 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 302
Score = 28.3 bits (60), Expect = 3.3
Identities = 21/71 (29%), Positives = 27/71 (38%), Gaps = 1/71 (1%)
Frame = -1
Query: 272 CMNLLCRIFLCPSEQRVLVRSSVFSIFAHRLFCRHWQKPDGLWL-CHGRFYLTAM*SARL 96
C L C + LC L+ S+FA LF H+ D L H F L +
Sbjct: 98 CFALFCELTLCSDLFSGLIGVLSASVFAQELFLLHFHSTDHSGLEGHYHFLLQLIAFVSF 157
Query: 95 SPEAWSPSVPR 63
S S S P+
Sbjct: 158 SSALASASFPK 168
>At4g01080.1 68417.m00146 expressed protein
Length = 442
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/46 (28%), Positives = 25/46 (54%), Gaps = 4/46 (8%)
Frame = -2
Query: 265 ISFVEFSYVLLSNGYWF---EVLYSPYLHTGCSADIGKSRM-DFGF 140
+ + +F YV++S G WF + + + TGC G++ + D G+
Sbjct: 240 VQYPKFDYVVISGGKWFLKTTIFHENNVVTGCHYCQGRNNLTDLGY 285
>At3g51760.1 68416.m05676 hypothetical protein hypothetical protein
F17M19.12 - Arabidopsis thaliana, EMBL:AC021665
Length = 228
Score = 27.9 bits (59), Expect = 4.4
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 318 ISTTGSVGSRRKTSQEPPQSSPLAWTTSSGAPRSST 425
+ST V + Q P S P W T+ G P++S+
Sbjct: 26 LSTKRHVTQEKLPGQFPEASVPFCWETAPGMPKNSS 61
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.5 bits (58), Expect = 5.8
Identities = 30/120 (25%), Positives = 48/120 (40%)
Frame = +2
Query: 71 RTATKPQEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQIWRIQNFEPIPVAQKDIGK 250
R P+E + SLS A+ KAK V R+ + W I I A K +
Sbjct: 134 RKEASPEECDQAVESLSSVKAKAKAKRLQESKKVARSIVQRAWAI--VLKIGPAIKAVAS 191
Query: 251 FYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQHRE 430
+ D + T +S HYW+G + ++ ++ L + L GG ++ RE
Sbjct: 192 MNRAD--WAKKLTHWKHEFVSTLKHYWLGTKLLWADTRISSRLLLKL---AGGKSLSRRE 246
>At3g21950.1 68416.m02769 S-adenosyl-L-methionine:carboxyl
methyltransferase family protein similar to
SAM:salicylic acid carboxyl methyltransferase (SAMT)
[GI:6002712][Clarkia breweri] and to SAM:benzoic acid
carboxyl methyltransferase
(BAMT)[GI:9789277][Antirrhinum majus]
Length = 335
Score = 27.5 bits (58), Expect = 5.8
Identities = 18/62 (29%), Positives = 23/62 (37%), Gaps = 2/62 (3%)
Frame = -1
Query: 257 CRIFLCPSEQRVLVRSSVFSIFAHRLFCRHW--QKPDGLWLCHGRFYLTAM*SARLSPEA 84
C + CP + S F H FC HW + PDGL YL + L
Sbjct: 89 CYVSGCPGSFYTRLFPSKSLHFVHSSFCLHWLSKVPDGLEENKKNVYLRSPCPPNLYESY 148
Query: 83 WS 78
W+
Sbjct: 149 WN 150
>At1g69295.1 68414.m07947 beta-1,3-glucanase-related low similarity
to elicitor inducible beta-1,3-glucanase NtEIG-E76
[Nicotiana tabacum] GI:11071974
Length = 222
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +3
Query: 315 GISTTGSVGSRRKTSQEPPQSSPLAWTTSSGAPRSST 425
G TTG+ + TS P +P T ++G P S T
Sbjct: 110 GTPTTGTPTTGTPTSGTPTTGTPTTGTPTTGTPTSGT 146
>At1g66910.1 68414.m07604 protein kinase, putative similar to
receptor serine/threonine kinase PR5K
gi|1235680|gb|AAC49208
Length = 666
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +3
Query: 261 EIHTSFYGRPRTVATIY-HGISTTGSVGSRRKTSQEPPQSS 380
E+ + YGR + +Y +G+ +G+R KTS E SS
Sbjct: 518 EVFSRVYGRVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSS 558
>At1g11480.1 68414.m01319 eukaryotic translation initiation
factor-related contains weak similarity to
Swiss-Prot:P23588 eukaryotic translation initiation
factor 4B (eIF-4B) [Homo sapiens]
Length = 578
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 47 VFLGSVWARTATKPQEISGPITSLSDKNARDKAKVHPAFANVGR 178
VF + W + +P + + P +S + +N DK+ P N+GR
Sbjct: 41 VFDRAPWGNSIGRPDQRARPSSSHAIRNLDDKSLFLPHNVNIGR 84
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,593,344
Number of Sequences: 28952
Number of extensions: 314535
Number of successful extensions: 928
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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