BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS307E06f (521 letters) Database: uniref50 1,657,284 sequences; 575,637,011 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UniRef50_Q7QCJ9 Cluster: Mediator of RNA polymerase II transcrip... 146 4e-34 UniRef50_Q9VNG0 Cluster: Mediator of RNA polymerase II transcrip... 113 2e-24 UniRef50_UPI0000584510 Cluster: PREDICTED: similar to Cofactor r... 97 2e-19 UniRef50_Q6P2C8 Cluster: Mediator of RNA polymerase II transcrip... 94 2e-18 UniRef50_A7RWD3 Cluster: Predicted protein; n=1; Nematostella ve... 43 0.004 UniRef50_Q2BDV8 Cluster: Putative uncharacterized protein; n=1; ... 33 5.2 UniRef50_Q2AUA2 Cluster: ErfK/YbiS/YcfS/YnhG; n=12; Bacillus|Rep... 33 5.2 UniRef50_A3LVI1 Cluster: Putative uncharacterized protein; n=1; ... 33 5.2 UniRef50_A1WZ25 Cluster: Outer membrane lipoprotein, Slp family ... 32 9.2 UniRef50_Q54L26 Cluster: Putative uncharacterized protein; n=1; ... 32 9.2 UniRef50_A7SG47 Cluster: Predicted protein; n=1; Nematostella ve... 32 9.2 UniRef50_A2EUN3 Cluster: Clan SB, family S8, subtilisin-like ser... 32 9.2 >UniRef50_Q7QCJ9 Cluster: Mediator of RNA polymerase II transcription subunit 27; n=5; Endopterygota|Rep: Mediator of RNA polymerase II transcription subunit 27 - Anopheles gambiae (African malaria mosquito) Length = 295 Score = 146 bits (353), Expect = 4e-34 Identities = 74/136 (54%), Positives = 97/136 (71%), Gaps = 5/136 (3%) Frame = +2 Query: 2 IHEYSSFAHTLLSQNSLKRSYINSGSTKRRGKLQSNHNVAPIQVDNVINNIDRSYNDMKI 181 +HEYS+FA TLL QNSLKRSY + TKRR L S+H P V+N+I +I YN+M + Sbjct: 109 LHEYSTFASTLLQQNSLKRSYYTN--TKRRRSLPSSHLATPQMVENLIGSIH--YNNMNL 164 Query: 182 TISRPFASNAVVQINLSHVLKAVVAFKGLLMEWVMVKGYGETL-----DLWTESRHHVFR 346 I+RPF +NA++ I ++ VL+A V KGLL+EWV VKGY E+L + WTESRH VFR Sbjct: 165 KIARPFMTNAILHITIARVLRAAVILKGLLIEWVTVKGYEESLLDGVDEQWTESRHQVFR 224 Query: 347 KVTENAHAAMXPFYSP 394 KV ++AH+AM F+SP Sbjct: 225 KVQDHAHSAMLHFFSP 240 Score = 32.3 bits (70), Expect = 6.9 Identities = 12/19 (63%), Positives = 14/19 (73%) Frame = +1 Query: 400 PELAVRXFMTWLHXYVNLF 456 PELA+R F+TW YV LF Sbjct: 243 PELAIRSFITWFRSYVTLF 261 >UniRef50_Q9VNG0 Cluster: Mediator of RNA polymerase II transcription subunit 27; n=1; Drosophila melanogaster|Rep: Mediator of RNA polymerase II transcription subunit 27 - Drosophila melanogaster (Fruit fly) Length = 293 Score = 113 bits (273), Expect = 2e-24 Identities = 60/134 (44%), Positives = 88/134 (65%), Gaps = 3/134 (2%) Frame = +2 Query: 2 IHEYSSFAHTLLSQNSLKRSYINSGSTKRRGKLQSNHNVAPIQVDNVINNIDRSYNDMKI 181 +H++S A L+QN+L+RSY N S KR+ S+ N P +D +++ I+ + Sbjct: 106 VHDHSFLAFNNLNQNTLRRSY-NYCSQKRQRLPFSSFNNDPDHIDKLLSEINTPPHT-SY 163 Query: 182 TISRPFASNAVVQINLSHVLKAVVAFKGLLMEWVMVKGYGETL---DLWTESRHHVFRKV 352 I RPF +NAV + +S+V+KA + FKG+L+EWV VKG+ E L DLW ESR+ VFRKV Sbjct: 164 RIFRPFGTNAVTIVTISNVMKAAIVFKGVLIEWVTVKGFDEPLEHDDLWAESRYEVFRKV 223 Query: 353 TENAHAAMXPFYSP 394 ++AH+AM F+SP Sbjct: 224 QDHAHSAMLHFFSP 237 >UniRef50_UPI0000584510 Cluster: PREDICTED: similar to Cofactor required for Sp1 transcriptional activation, subunit 8; n=1; Strongylocentrotus purpuratus|Rep: PREDICTED: similar to Cofactor required for Sp1 transcriptional activation, subunit 8 - Strongylocentrotus purpuratus Length = 244 Score = 97.1 bits (231), Expect = 2e-19 Identities = 52/142 (36%), Positives = 90/142 (63%), Gaps = 8/142 (5%) Frame = +2 Query: 2 IHEYSSFAHTLLSQNSLKRSYINSGS-TKRRGKLQSNHNVAPIQVDNVINNIDRSYNDMK 178 + E+S+ + L+ +S+KRS + GS KRR +N N++ VDN++ N++R ++D+K Sbjct: 53 LREHSAHSQAFLNNSSIKRSSGSGGSPAKRRKTAPTNLNISTQAVDNLVMNVNRQFHDVK 112 Query: 179 ITISRPFASNAVVQINLSHVLKAVVAFKGLLMEWVMVKGYGE-------TLDLWTESRHH 337 IT + + V+Q+ +S L+A++ +GLL+E V+++ + E TLDLWT S++ Sbjct: 113 ITKIQ--LNPTVLQVYVSRTLQAIIVLRGLLIERVLIRAHHENTLNDDGTLDLWTASKYE 170 Query: 338 VFRKVTENAHAAMXPFYSPXYQ 403 VF+K+TE+A AA +Y P Q Sbjct: 171 VFQKITEHATAASLRYYLPTMQ 192 >UniRef50_Q6P2C8 Cluster: Mediator of RNA polymerase II transcription subunit 27; n=34; Euteleostomi|Rep: Mediator of RNA polymerase II transcription subunit 27 - Homo sapiens (Human) Length = 311 Score = 93.9 bits (223), Expect = 2e-18 Identities = 55/137 (40%), Positives = 82/137 (59%), Gaps = 9/137 (6%) Frame = +2 Query: 11 YSSFAHTLLSQNSLKRSYINSG-STKRRGKLQSNHNVAPIQ-VDNVINNIDRSYNDMKIT 184 ++ A LL+Q SLKRS G S KRR K Q V P Q VD+VI+ IDR + +M I Sbjct: 120 HAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIH 179 Query: 185 ISRPFASNAVVQINLSHVLKAVVAFKGLLMEWVMVKGYGET-------LDLWTESRHHVF 343 +SRP ++A++ + L VLK +V + L ++ +VKGY E LD+W++S + VF Sbjct: 180 LSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVF 239 Query: 344 RKVTENAHAAMXPFYSP 394 +KVT++A A+ + P Sbjct: 240 QKVTDHATTALLHYQLP 256 Score = 31.9 bits (69), Expect = 9.2 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = +1 Query: 400 PELAVRXFMTWLHXYVNLFR 459 P++ VR FMTWL Y+ LF+ Sbjct: 259 PDVVVRSFMTWLRSYIKLFQ 278 >UniRef50_A7RWD3 Cluster: Predicted protein; n=1; Nematostella vectensis|Rep: Predicted protein - Nematostella vectensis Length = 307 Score = 43.2 bits (97), Expect = 0.004 Identities = 23/96 (23%), Positives = 53/96 (55%), Gaps = 6/96 (6%) Frame = +2 Query: 122 PIQVDNVINNIDRSYNDMKITISRPFASNAVVQINLSHVLKAVVAFKGLLMEWVMVKGYG 301 P N++ + + +++++ R S A+ ++ + KA++ KG+ ++ V ++G Sbjct: 150 PPLTPNILEALKAKHINLRVSGYRICGSTAL-EVTVPKTFKAIIMLKGVGIDQVKIRGLY 208 Query: 302 ETL------DLWTESRHHVFRKVTENAHAAMXPFYS 391 E D+W+ S+H VF+K+T+ A++A +Y+ Sbjct: 209 ECSLMEKKDDIWSPSKHIVFQKLTDYANSAALHYYN 244 >UniRef50_Q2BDV8 Cluster: Putative uncharacterized protein; n=1; Bacillus sp. NRRL B-14911|Rep: Putative uncharacterized protein - Bacillus sp. NRRL B-14911 Length = 107 Score = 32.7 bits (71), Expect = 5.2 Identities = 16/50 (32%), Positives = 29/50 (58%) Frame = +2 Query: 2 IHEYSSFAHTLLSQNSLKRSYINSGSTKRRGKLQSNHNVAPIQVDNVINN 151 ++ Y S+AH + +NSL R Y + +TK + ++A I + ++INN Sbjct: 59 VYFYDSYAHQVYDRNSLARLY-DYWATKNVRSFAAARDMASIDIRSIINN 107 >UniRef50_Q2AUA2 Cluster: ErfK/YbiS/YcfS/YnhG; n=12; Bacillus|Rep: ErfK/YbiS/YcfS/YnhG - Bacillus weihenstephanensis KBAB4 Length = 481 Score = 32.7 bits (71), Expect = 5.2 Identities = 24/77 (31%), Positives = 37/77 (48%), Gaps = 3/77 (3%) Frame = +2 Query: 110 HNVAPIQVDNVINNIDRSYNDMKITISRPFASNAVVQI-NLSHVLKAVVAFKGLLMEWVM 286 + V P++ ++I N S DMK+TI N + +I N L +FK + Sbjct: 240 NEVYPLKAKDLIQNASMS-KDMKVTIDASDIKNKIAEINNAKSTLNKNFSFKTHSGSVIS 298 Query: 287 VK--GYGETLDLWTESR 331 VK GYG LD+ E++ Sbjct: 299 VKGQGYGWALDVEKETK 315 >UniRef50_A3LVI1 Cluster: Putative uncharacterized protein; n=1; Pichia stipitis|Rep: Putative uncharacterized protein - Pichia stipitis (Yeast) Length = 730 Score = 32.7 bits (71), Expect = 5.2 Identities = 19/53 (35%), Positives = 28/53 (52%) Frame = +2 Query: 56 RSYINSGSTKRRGKLQSNHNVAPIQVDNVINNIDRSYNDMKITISRPFASNAV 214 R Y S S K +G SN++++P+ +D IN +D S D I ASN + Sbjct: 172 RGYSYSNSLKTKGLDLSNYDLSPLDID--INRLDASRLDESIFALSSAASNVI 222 >UniRef50_A1WZ25 Cluster: Outer membrane lipoprotein, Slp family precursor; n=2; Ectothiorhodospiraceae|Rep: Outer membrane lipoprotein, Slp family precursor - Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospirahalophila (strain DSM 244 / SL1)) Length = 189 Score = 31.9 bits (69), Expect = 9.2 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = +2 Query: 296 YGETLDLWTESRHHVFRKVTENAHAAMXPFYSPXYQSW 409 YG T + + HH++++ E A P+Y P Y W Sbjct: 129 YGYTYPVVEVAAHHLWQERAEPRQVAPRPYYDPWYDPW 166 >UniRef50_Q54L26 Cluster: Putative uncharacterized protein; n=1; Dictyostelium discoideum AX4|Rep: Putative uncharacterized protein - Dictyostelium discoideum AX4 Length = 804 Score = 31.9 bits (69), Expect = 9.2 Identities = 16/62 (25%), Positives = 37/62 (59%) Frame = +2 Query: 131 VDNVINNIDRSYNDMKITISRPFASNAVVQINLSHVLKAVVAFKGLLMEWVMVKGYGETL 310 ++++I +ID+S K+TIS P S + ++ + + ++++ A G+L+E +++ L Sbjct: 332 IESLIQSIDKSTKTFKLTISHPLVSRSTIKNSETITMESIKA--GMLVETKVLRVLSNGL 389 Query: 311 DL 316 L Sbjct: 390 HL 391 >UniRef50_A7SG47 Cluster: Predicted protein; n=1; Nematostella vectensis|Rep: Predicted protein - Nematostella vectensis Length = 493 Score = 31.9 bits (69), Expect = 9.2 Identities = 26/102 (25%), Positives = 47/102 (46%), Gaps = 14/102 (13%) Frame = +2 Query: 38 SQNSLKRSYINSGSTKRRGKLQSNHNVAPIQVDNVINNIDRSYN---DMKITISRPFA-- 202 SQ S+ +++ SG++ + + + +P V V N+DR+ ++ + RP Sbjct: 197 SQASISTAHVKSGTSALKWEWPGSGATSPSMVLKVARNVDRNTRRKGGIRFWMYRPHTQV 256 Query: 203 ---SNAVVQIN------LSHVLKAVVAFKGLLMEWVMVKGYG 301 +NA V+I LS ++K + F+G WV K G Sbjct: 257 ANVANATVEITFNTDAGLSKIIKVNLGFEGWRTAWVTYKELG 298 >UniRef50_A2EUN3 Cluster: Clan SB, family S8, subtilisin-like serine peptidase; n=1; Trichomonas vaginalis G3|Rep: Clan SB, family S8, subtilisin-like serine peptidase - Trichomonas vaginalis G3 Length = 773 Score = 31.9 bits (69), Expect = 9.2 Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 6/68 (8%) Frame = +2 Query: 95 KLQSNHNVAPIQVDNVINNIDRSYNDMKITISRPFASNAVV-QINL-----SHVLKAVVA 256 K+Q N+ V+ V N D Y D++I + P ++A+V Q+++ +++K + Sbjct: 401 KIQMKDNLFIETVELVFNIADEDYGDIRIEVISPSKTHAIVKQLSIMRNVPKNMIKRTIT 460 Query: 257 FKGLLMEW 280 + L EW Sbjct: 461 IRNFLGEW 468 Database: uniref50 Posted date: Oct 5, 2007 11:19 AM Number of letters in database: 575,637,011 Number of sequences in database: 1,657,284 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 422,668,561 Number of Sequences: 1657284 Number of extensions: 7588619 Number of successful extensions: 17136 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 16685 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 17122 length of database: 575,637,011 effective HSP length: 95 effective length of database: 418,195,031 effective search space used: 32619212418 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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