Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS307D11f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g20650.1 68414.m02587 protein kinase family protein contains ... 29 1.9
At4g26720.1 68417.m03851 serine/threonine protein phosphatase PP... 29 2.5
At4g18425.1 68417.m02734 expressed protein contains Pfam profile... 28 3.3
At5g01260.2 68418.m00035 glycoside hydrolase starch-binding doma... 28 4.4
At5g01260.1 68418.m00034 glycoside hydrolase starch-binding doma... 28 4.4
At5g53440.1 68418.m06641 expressed protein 27 5.8
At2g39630.2 68415.m04859 glycosyl transferase family 2 protein s... 27 5.8
At2g39630.1 68415.m04858 glycosyl transferase family 2 protein s... 27 5.8
>At1g20650.1 68414.m02587 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 585
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/37 (32%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +3
Query: 150 RWKNKLFVTVPKWKNGV-ASSLNYVDLNGTSDQLLKP 257
+W + ++V P WKN + + L +V + T D +L P
Sbjct: 143 QWSSYIYVLPPPWKNIIEETKLRFVGTSDTGDIVLSP 179
>At4g26720.1 68417.m03851 serine/threonine protein phosphatase PP-X
isozyme 1 (PPX1) identical to SP|P48529 Serine/threonine
protein phosphatase PP-X isozyme 1 (EC 3.1.3.16)
{Arabidopsis thaliana}; contains Pfam profile PF00149:
Ser/Thr protein phosphatase
Length = 305
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = +3
Query: 366 WVMDTGLADIWGAGNQIVRPSIVIFDLKTDQLLHRYFFKLEDMKEDS 506
W+ D+ + +W A N R V L+ D+ L++ F + ++DS
Sbjct: 245 WMFDSQIVTVWSAPNYCYRCGNVASILELDENLNKEFRVFDAAQQDS 291
>At4g18425.1 68417.m02734 expressed protein contains Pfam profile
PF05078: Protein of unknown function (DUF679)
Length = 213
Score = 28.3 bits (60), Expect = 3.3
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 132 LPLGLGRWKNKLFVTVPKWKNGVASSLN 215
LP+G+G + + LF T P +NG+ L+
Sbjct: 184 LPVGVGVFSSMLFATFPTTRNGIGFPLS 211
>At5g01260.2 68418.m00035 glycoside hydrolase starch-binding
domain-containing protein low similarity to SP|P31797
Cyclomaltodextrin glucanotransferase precursor (EC
2.4.1.19) (Cyclodextrin-glycosyltransferase) (CGTase)
{Bacillus stearothermophilus}; contains Pfam profile
PF00686: Starch binding domain
Length = 385
Score = 27.9 bits (59), Expect = 4.4
Identities = 21/77 (27%), Positives = 43/77 (55%), Gaps = 6/77 (7%)
Frame = +3
Query: 129 NLPLGLGRWKNKLFVTVPKWKNGVASSLNYVDLNGT-SDQLLKPYP----SLKDNFIPDS 293
+LP G+ R+ ++F +VP+ + ++ L+ S ++LKP P S+KD+ +
Sbjct: 20 DLPPGIYRYGTEIFTSVPR---KTFHHIKFLRLDSAQSSRILKPVPLRSSSIKDSQVNVE 76
Query: 294 AKDL-PSNKTIISVFRI 341
+++ SNKT+ F++
Sbjct: 77 DEEIEASNKTVRVRFQL 93
>At5g01260.1 68418.m00034 glycoside hydrolase starch-binding
domain-containing protein low similarity to SP|P31797
Cyclomaltodextrin glucanotransferase precursor (EC
2.4.1.19) (Cyclodextrin-glycosyltransferase) (CGTase)
{Bacillus stearothermophilus}; contains Pfam profile
PF00686: Starch binding domain
Length = 306
Score = 27.9 bits (59), Expect = 4.4
Identities = 21/77 (27%), Positives = 43/77 (55%), Gaps = 6/77 (7%)
Frame = +3
Query: 129 NLPLGLGRWKNKLFVTVPKWKNGVASSLNYVDLNGT-SDQLLKPYP----SLKDNFIPDS 293
+LP G+ R+ ++F +VP+ + ++ L+ S ++LKP P S+KD+ +
Sbjct: 20 DLPPGIYRYGTEIFTSVPR---KTFHHIKFLRLDSAQSSRILKPVPLRSSSIKDSQVNVE 76
Query: 294 AKDL-PSNKTIISVFRI 341
+++ SNKT+ F++
Sbjct: 77 DEEIEASNKTVRVRFQL 93
>At5g53440.1 68418.m06641 expressed protein
Length = 1181
Score = 27.5 bits (58), Expect = 5.8
Identities = 13/66 (19%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = +3
Query: 78 PAERENAIKSGDFVPANNLPLGLGRWKNKLFVTVPKWKNGVASS---LNYVDLNGTSDQL 248
P E +N + +G + +GR ++ ++ VP W + +++ +V +G +
Sbjct: 614 PREEDNRVNTGGRYKRGGVDAMMGRGQSNMWRGVPSWPSPLSNGYFPFQHVPPHGAFQTM 673
Query: 249 LKPYPS 266
+ +PS
Sbjct: 674 MPQFPS 679
>At2g39630.2 68415.m04859 glycosyl transferase family 2 protein
similar to dolichyl-phosphate beta-glucosyltransferase
from Saccharomyces cerevisiae [SP|P40350]; contains Pfam
glycosyltransferase group 2 domain PF00535
Length = 236
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/22 (45%), Positives = 18/22 (81%)
Frame = +1
Query: 334 LEFTLTLVTDFGLWILVLLIFG 399
+EF +T V +F LW+L++++FG
Sbjct: 1 MEFLVT-VAEFSLWLLLIVLFG 21
>At2g39630.1 68415.m04858 glycosyl transferase family 2 protein
similar to dolichyl-phosphate beta-glucosyltransferase
from Saccharomyces cerevisiae [SP|P40350]; contains Pfam
glycosyltransferase group 2 domain PF00535
Length = 336
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/22 (45%), Positives = 18/22 (81%)
Frame = +1
Query: 334 LEFTLTLVTDFGLWILVLLIFG 399
+EF +T V +F LW+L++++FG
Sbjct: 1 MEFLVT-VAEFSLWLLLIVLFG 21
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,643,732
Number of Sequences: 28952
Number of extensions: 251358
Number of successful extensions: 669
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 669
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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