BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS307D03f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 146 8e-36 At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 144 3e-35 At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 141 2e-34 At5g63900.1 68418.m08023 PHD finger family protein contains Pfam... 30 0.82 At4g34630.1 68417.m04918 expressed protein 29 1.9 At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 29 1.9 At1g66070.1 68414.m07499 translation initiation factor-related s... 29 1.9 At3g63310.1 68416.m07121 expressed protein low similarity to N-m... 28 3.3 At5g12000.1 68418.m01403 protein kinase family protein contains ... 28 4.4 At1g52990.1 68414.m05997 thioredoxin family protein similar to S... 27 7.7 At1g50820.1 68414.m05715 hypothetical protein 27 7.7 At1g01350.1 68414.m00050 zinc finger (CCCH-type/C3HC4-type RING ... 27 7.7 >At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum tuberosum] Length = 135 Score = 146 bits (354), Expect = 8e-36 Identities = 63/133 (47%), Positives = 94/133 (70%), Gaps = 1/133 (0%) Frame = -3 Query: 438 EFGTRGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKI 259 +F + K V++L GRYAG+KA+++K++D+GTSD+ YGH VAG+ +YP KV ++ K Sbjct: 3 KFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKT 62 Query: 258 HKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEERYK 82 K+S++K F+K+VNY HLMPTRYT+D ++ + D LK K+ + + EER+K Sbjct: 63 AKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFK 122 Query: 81 SGKNKWFFQKLRF 43 +GKN+WFF KLRF Sbjct: 123 TGKNRWFFTKLRF 135 >At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) Length = 135 Score = 144 bits (349), Expect = 3e-35 Identities = 61/133 (45%), Positives = 93/133 (69%), Gaps = 1/133 (0%) Frame = -3 Query: 438 EFGTRGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKI 259 +F + K V++L GRYAG+KA+++K++D+G D+PYGH VAG+ +YP KV ++ K Sbjct: 3 KFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKT 62 Query: 258 HKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEERYK 82 K+S++K F+K+VNY HLMPTRYT+D ++ + D L+ K+ + + EER+K Sbjct: 63 AKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFK 122 Query: 81 SGKNKWFFQKLRF 43 +GKN+WFF KLRF Sbjct: 123 TGKNRWFFTKLRF 135 >At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) Length = 135 Score = 141 bits (342), Expect = 2e-34 Identities = 61/128 (47%), Positives = 88/128 (68%), Gaps = 1/128 (0%) Frame = -3 Query: 423 GKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 244 GK V++L GRY G+KA++VK++D+GT +K YGH VAG+ +YP KV ++ K K+S+ Sbjct: 8 GKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSR 67 Query: 243 IKPFVKVVNYNHLMPTRYTVDFSFEK-FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 67 +K F KV+NY H+MPTRYT+D + SA + K+ + +FEER+K+GKN+ Sbjct: 68 VKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNR 127 Query: 66 WFFQKLRF 43 WFF KLRF Sbjct: 128 WFFTKLRF 135 >At5g63900.1 68418.m08023 PHD finger family protein contains Pfam domain, PF00628: PHD-finger Length = 557 Score = 30.3 bits (65), Expect = 0.82 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = -3 Query: 117 FNTRVRFEERYKSGKNKWFF 58 +N R + E+RYKS K KWF+ Sbjct: 58 YNKRNKKEQRYKSPKGKWFY 77 >At4g34630.1 68417.m04918 expressed protein Length = 199 Score = 29.1 bits (62), Expect = 1.9 Identities = 11/38 (28%), Positives = 21/38 (55%) Frame = -3 Query: 357 DEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 244 D+G D+ Y ++ + PR V K++G+ ++ K K Sbjct: 130 DDGEEDREYDDSYDLDEELVPRSVSKKVGRQRMRKLGK 167 >At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-related similar to CCAAT-box-binding transcription factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701) [Homo sapiens], GB:P53569 [Mus musculus] Length = 1056 Score = 29.1 bits (62), Expect = 1.9 Identities = 17/52 (32%), Positives = 22/52 (42%) Frame = -3 Query: 279 RMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKK 124 R K K KR + PF + Y HL+ D K K +P K+KK Sbjct: 1000 RSKKKKKEKRKRKSPFASLEEYKHLIDQDEKED---SKTKRKATSEPTKKKK 1048 >At1g66070.1 68414.m07499 translation initiation factor-related similar to Eukaryotic translation initiation factor 3 subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j) (Swiss-Prot:O75822) [Homo sapiens] Length = 226 Score = 29.1 bits (62), Expect = 1.9 Identities = 18/60 (30%), Positives = 29/60 (48%) Frame = -3 Query: 246 KIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 67 +IKP+ K +Y L+ T + S A D+KD A N +++ E+ +GK K Sbjct: 139 RIKPYEKSYHYIALLKT--IMRLSLTNMKAADVKDVASSITTIANEKLKAEKEAAAGKKK 196 >At3g63310.1 68416.m07121 expressed protein low similarity to N-methyl-D-aspartate receptor-associated protein [Drosophila melanogaster] GI:567104; contains Pfam profile PF01027: Uncharacterized protein family UPF0005 Length = 239 Score = 28.3 bits (60), Expect = 3.3 Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 1/74 (1%) Frame = +2 Query: 83 LYLSSKRTRVLKRSFLRLAGSFRSFALNFS-KLKSTV*RVGIK*L*FTTLTKGFILDLLW 259 LY + L+ SF+R S S L + + +TV +V + FTT T GF L +L Sbjct: 15 LYPMMSESPELRWSFIRKVYSIISIQLLVTIAVAATVVKVHSISVFFTTTTAGFALYILL 74 Query: 260 ILFFPILLCTFRGY 301 IL I++C Y Sbjct: 75 ILTPLIVMCPLYYY 88 >At5g12000.1 68418.m01403 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 703 Score = 27.9 bits (59), Expect = 4.4 Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = -3 Query: 306 DRYPRKVHKRMGKNKIHKR-SKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKR 130 DR PR +R G+N + +R S K V H +PT ++DF++E K ++ R Sbjct: 188 DRSPRS--QRNGRNTVPERYSHENKGFKPVREMHKIPTNGSLDFNYEFRQGKGQRNSTGR 245 >At1g52990.1 68414.m05997 thioredoxin family protein similar to SP|P48384 Thioredoxin M-type, chloroplast precursor (TRX-M) {Pisum sativum}; contains Pfam profile PF00085: Thioredoxin Length = 313 Score = 27.1 bits (57), Expect = 7.7 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 7/37 (18%) Frame = -2 Query: 466 VLKPNVPSCRIR-------HEG*SSAGPKWPVRGSQG 377 V +VPSCR++ HEG +G W +R SQG Sbjct: 38 VSSSSVPSCRVKCNYDASHHEGDVVSGLGWLIRNSQG 74 >At1g50820.1 68414.m05715 hypothetical protein Length = 528 Score = 27.1 bits (57), Expect = 7.7 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = -2 Query: 370 SQELRRRYLRQAIRACLRRWYRQVPPESAQED 275 S RR+Y++QA R + +QVP ++ ++D Sbjct: 476 STNKRRKYMKQAHRGTMGLCQKQVPSDNDEDD 507 >At1g01350.1 68414.m00050 zinc finger (CCCH-type/C3HC4-type RING finger) family protein similar to SP|O15541 Zinc finger protein 183 {Homo sapiens}; contains Pfam profiles PF04396: Protein of unknown function, DUF537, PF00097: Zinc finger, C3HC4 type (RING finger), PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 586 Score = 27.1 bits (57), Expect = 7.7 Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +3 Query: 378 PCDPRTGHLG-PALLYPSCRIRHEGTFGFSTRTVNQ*NITNENSFRSQN 521 P + + G +G PALL CR +G FST ++ + +R+++ Sbjct: 520 PQEDKCGEMGEPALLCEQCRFTVQGFENFSTHLKSEEHAHESQYYRNED 568 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,213,419 Number of Sequences: 28952 Number of extensions: 228726 Number of successful extensions: 664 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 653 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 661 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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