BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS307D03f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 146 8e-36
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 144 3e-35
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 141 2e-34
At5g63900.1 68418.m08023 PHD finger family protein contains Pfam... 30 0.82
At4g34630.1 68417.m04918 expressed protein 29 1.9
At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 29 1.9
At1g66070.1 68414.m07499 translation initiation factor-related s... 29 1.9
At3g63310.1 68416.m07121 expressed protein low similarity to N-m... 28 3.3
At5g12000.1 68418.m01403 protein kinase family protein contains ... 28 4.4
At1g52990.1 68414.m05997 thioredoxin family protein similar to S... 27 7.7
At1g50820.1 68414.m05715 hypothetical protein 27 7.7
At1g01350.1 68414.m00050 zinc finger (CCCH-type/C3HC4-type RING ... 27 7.7
>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
tuberosum]
Length = 135
Score = 146 bits (354), Expect = 8e-36
Identities = 63/133 (47%), Positives = 94/133 (70%), Gaps = 1/133 (0%)
Frame = -3
Query: 438 EFGTRGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKI 259
+F + K V++L GRYAG+KA+++K++D+GTSD+ YGH VAG+ +YP KV ++ K
Sbjct: 3 KFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKT 62
Query: 258 HKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEERYK 82
K+S++K F+K+VNY HLMPTRYT+D ++ + D LK K+ + + EER+K
Sbjct: 63 AKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFK 122
Query: 81 SGKNKWFFQKLRF 43
+GKN+WFF KLRF
Sbjct: 123 TGKNRWFFTKLRF 135
>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
Length = 135
Score = 144 bits (349), Expect = 3e-35
Identities = 61/133 (45%), Positives = 93/133 (69%), Gaps = 1/133 (0%)
Frame = -3
Query: 438 EFGTRGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKI 259
+F + K V++L GRYAG+KA+++K++D+G D+PYGH VAG+ +YP KV ++ K
Sbjct: 3 KFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKT 62
Query: 258 HKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEERYK 82
K+S++K F+K+VNY HLMPTRYT+D ++ + D L+ K+ + + EER+K
Sbjct: 63 AKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFK 122
Query: 81 SGKNKWFFQKLRF 43
+GKN+WFF KLRF
Sbjct: 123 TGKNRWFFTKLRF 135
>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)
Length = 135
Score = 141 bits (342), Expect = 2e-34
Identities = 61/128 (47%), Positives = 88/128 (68%), Gaps = 1/128 (0%)
Frame = -3
Query: 423 GKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 244
GK V++L GRY G+KA++VK++D+GT +K YGH VAG+ +YP KV ++ K K+S+
Sbjct: 8 GKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSR 67
Query: 243 IKPFVKVVNYNHLMPTRYTVDFSFEK-FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 67
+K F KV+NY H+MPTRYT+D + SA + K+ + +FEER+K+GKN+
Sbjct: 68 VKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNR 127
Query: 66 WFFQKLRF 43
WFF KLRF
Sbjct: 128 WFFTKLRF 135
>At5g63900.1 68418.m08023 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 557
Score = 30.3 bits (65), Expect = 0.82
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -3
Query: 117 FNTRVRFEERYKSGKNKWFF 58
+N R + E+RYKS K KWF+
Sbjct: 58 YNKRNKKEQRYKSPKGKWFY 77
>At4g34630.1 68417.m04918 expressed protein
Length = 199
Score = 29.1 bits (62), Expect = 1.9
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = -3
Query: 357 DEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 244
D+G D+ Y ++ + PR V K++G+ ++ K K
Sbjct: 130 DDGEEDREYDDSYDLDEELVPRSVSKKVGRQRMRKLGK 167
>At1g72440.1 68414.m08377 CCAAT-box-binding transcription
factor-related similar to CCAAT-box-binding transcription
factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701)
[Homo sapiens], GB:P53569 [Mus musculus]
Length = 1056
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = -3
Query: 279 RMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKK 124
R K K KR + PF + Y HL+ D K K +P K+KK
Sbjct: 1000 RSKKKKKEKRKRKSPFASLEEYKHLIDQDEKED---SKTKRKATSEPTKKKK 1048
>At1g66070.1 68414.m07499 translation initiation factor-related
similar to Eukaryotic translation initiation factor 3
subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
(Swiss-Prot:O75822) [Homo sapiens]
Length = 226
Score = 29.1 bits (62), Expect = 1.9
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = -3
Query: 246 KIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 67
+IKP+ K +Y L+ T + S A D+KD A N +++ E+ +GK K
Sbjct: 139 RIKPYEKSYHYIALLKT--IMRLSLTNMKAADVKDVASSITTIANEKLKAEKEAAAGKKK 196
>At3g63310.1 68416.m07121 expressed protein low similarity to
N-methyl-D-aspartate receptor-associated protein
[Drosophila melanogaster] GI:567104; contains Pfam
profile PF01027: Uncharacterized protein family UPF0005
Length = 239
Score = 28.3 bits (60), Expect = 3.3
Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 1/74 (1%)
Frame = +2
Query: 83 LYLSSKRTRVLKRSFLRLAGSFRSFALNFS-KLKSTV*RVGIK*L*FTTLTKGFILDLLW 259
LY + L+ SF+R S S L + + +TV +V + FTT T GF L +L
Sbjct: 15 LYPMMSESPELRWSFIRKVYSIISIQLLVTIAVAATVVKVHSISVFFTTTTAGFALYILL 74
Query: 260 ILFFPILLCTFRGY 301
IL I++C Y
Sbjct: 75 ILTPLIVMCPLYYY 88
>At5g12000.1 68418.m01403 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 703
Score = 27.9 bits (59), Expect = 4.4
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -3
Query: 306 DRYPRKVHKRMGKNKIHKR-SKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKR 130
DR PR +R G+N + +R S K V H +PT ++DF++E K ++ R
Sbjct: 188 DRSPRS--QRNGRNTVPERYSHENKGFKPVREMHKIPTNGSLDFNYEFRQGKGQRNSTGR 245
>At1g52990.1 68414.m05997 thioredoxin family protein similar to
SP|P48384 Thioredoxin M-type, chloroplast precursor
(TRX-M) {Pisum sativum}; contains Pfam profile PF00085:
Thioredoxin
Length = 313
Score = 27.1 bits (57), Expect = 7.7
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 7/37 (18%)
Frame = -2
Query: 466 VLKPNVPSCRIR-------HEG*SSAGPKWPVRGSQG 377
V +VPSCR++ HEG +G W +R SQG
Sbjct: 38 VSSSSVPSCRVKCNYDASHHEGDVVSGLGWLIRNSQG 74
>At1g50820.1 68414.m05715 hypothetical protein
Length = 528
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -2
Query: 370 SQELRRRYLRQAIRACLRRWYRQVPPESAQED 275
S RR+Y++QA R + +QVP ++ ++D
Sbjct: 476 STNKRRKYMKQAHRGTMGLCQKQVPSDNDEDD 507
>At1g01350.1 68414.m00050 zinc finger (CCCH-type/C3HC4-type RING
finger) family protein similar to SP|O15541 Zinc finger
protein 183 {Homo sapiens}; contains Pfam profiles
PF04396: Protein of unknown function, DUF537, PF00097:
Zinc finger, C3HC4 type (RING finger), PF00642: Zinc
finger C-x8-C-x5-C-x3-H type (and similar)
Length = 586
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +3
Query: 378 PCDPRTGHLG-PALLYPSCRIRHEGTFGFSTRTVNQ*NITNENSFRSQN 521
P + + G +G PALL CR +G FST ++ + +R+++
Sbjct: 520 PQEDKCGEMGEPALLCEQCRFTVQGFENFSTHLKSEEHAHESQYYRNED 568
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,213,419
Number of Sequences: 28952
Number of extensions: 228726
Number of successful extensions: 664
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 653
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 661
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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