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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS307B03f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g50170.1 68416.m05485 hypothetical protein contains Pfam prof...    31   0.62 
At3g20720.1 68416.m02622 expressed protein                             29   2.5  
At2g25640.1 68415.m03073 transcription elongation factor-related...    29   2.5  
At5g43840.1 68418.m05360 heat shock transcription factor family ...    28   3.3  
At3g56990.1 68416.m06344 glycine-rich protein conserved hypothet...    28   3.3  
At1g80750.1 68414.m09474 60S ribosomal protein L7 (RPL7A) simila...    28   4.4  
At1g69920.1 68414.m08046 glutathione S-transferase, putative sim...    28   4.4  
At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA li...    28   4.4  
At1g30420.1 68414.m03718 ATP-binding cassette transport protein,...    27   5.8  
At1g05150.1 68414.m00518 calcium-binding EF hand family protein ...    27   7.7  

>At3g50170.1 68416.m05485 hypothetical protein contains Pfam profile
           PF03140: Plant protein of unknown function
          Length = 541

 Score = 30.7 bits (66), Expect = 0.62
 Identities = 16/31 (51%), Positives = 21/31 (67%)
 Frame = +1

Query: 157 TPNVQNLLKRLTRPDFTKVFRKRNNQGLHAL 249
           TPN ++LLKRLTR   T+V  KR  Q +H +
Sbjct: 333 TPNTRSLLKRLTRN--TRVVDKRQQQLVHCV 361


>At3g20720.1 68416.m02622 expressed protein 
          Length = 1083

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +2

Query: 248 LKLPNISFSRKKSLNWKLLKLMKKPMCF 331
           L + N+SF+   +L  +LL L K P CF
Sbjct: 468 LHIENLSFAESPTLKVRLLNLEKDPACF 495


>At2g25640.1 68415.m03073 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 643

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 23/70 (32%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
 Frame = +1

Query: 292 LEIAKANEKADVLLQIPPVLKIHKPI-DEVLSRDPALIGYDTSKYLFTDITFGIANEERI 468
           L IAK ++ A V   + P ++ +    D V S+D  L G D S  L +DI F +  +  +
Sbjct: 190 LSIAKTSD-AQVPEAVKPFVQDNVSYSDNVFSKDDLLQGNDLSWALESDIEFTVNCQNEM 248

Query: 469 IIERDTDGTL 498
           I     DG+L
Sbjct: 249 IGAMANDGSL 258


>At5g43840.1 68418.m05360 heat shock transcription factor family
           protein contains Pfam profile: PF00447 HSF-type
           DNA-binding domain
          Length = 282

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = +1

Query: 175 LLKRLTRPDFTKVFRKRNNQGL 240
           LLK++ +P F +  RKRN QG+
Sbjct: 173 LLKKIKKPSFLQSLRKRNLQGI 194


>At3g56990.1 68416.m06344 glycine-rich protein conserved
           hypothetical protein SPCC330.09 - Schizosaccharomyces
           pombe, PIR:T41319
          Length = 711

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 19/63 (30%), Positives = 32/63 (50%)
 Frame = +1

Query: 103 NIVPSFYDGEQDPAPKFFTPNVQNLLKRLTRPDFTKVFRKRNNQGLHALKTPKYKFLTKE 282
           +++PS++  E  PAPK+ +P ++NL + L     T ++               YKFL  E
Sbjct: 341 SLIPSYFIPELGPAPKWCSP-LENLTEELEESAQTTIY-------------DNYKFLAME 386

Query: 283 ELE 291
           +LE
Sbjct: 387 DLE 389


>At1g80750.1 68414.m09474 60S ribosomal protein L7 (RPL7A) similar
           to ribosomal protein L7 GB:AAA03081 GI:307388 from [Homo
           sapiens]
          Length = 247

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 14/43 (32%), Positives = 26/43 (60%)
 Frame = +1

Query: 259 KYKFLTKEELELEIAKANEKADVLLQIPPVLKIHKPIDEVLSR 387
           ++K L  E+L  EIA+  +    +++    LK++KP+ +VL R
Sbjct: 179 EHKILGIEDLVNEIARVGDHFREVMRFLGPLKLNKPVADVLHR 221


>At1g69920.1 68414.m08046 glutathione S-transferase, putative
           similar to glutathione transferase GB:CAA09188
           [Alopecurus myosuroides]; supported by cDNA gi:15451157
           gb:AY050343.
          Length = 254

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +2

Query: 209 KFSARETIKVYMPLKLPNISFSRKK 283
           KF A E +K YMP     I F++KK
Sbjct: 227 KFRAHEAVKPYMPTVAEFIEFAKKK 251


>At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA
           ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA
           synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS)
           {Arabidopsis thaliana}
          Length = 1108

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 9/15 (60%), Positives = 12/15 (80%)
 Frame = -3

Query: 162 WCKKLWSRVLFAIVK 118
           WC KLW+ V FA++K
Sbjct: 782 WCNKLWNAVRFAMMK 796


>At1g30420.1 68414.m03718 ATP-binding cassette transport protein,
           putative contains Pfam profiles PF00005: ABC
           transporter, PF00664: ABC transporter transmembrane
           region
          Length = 1488

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +1

Query: 337 IPPVLKIHKPIDEVLSRDPALIGYDTSKYLFTDITFGI 450
           + PV+  H  +  ++  DPA +GY  +  +F  +TFG+
Sbjct: 319 VGPVILSHI-LQSMIEGDPAWVGYVYAFLIFFGVTFGV 355


>At1g05150.1 68414.m00518 calcium-binding EF hand family protein low
           similarity to O-linked GlcNAc transferase [Homo sapiens]
            GI:2266994; contains Pfam profiles PF00036: EF hand,
           PF00515: TPR Domain
          Length = 808

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
 Frame = +1

Query: 103 NIVPSFYDGEQDPAPKFFTPNVQNLLKRL---TRPDFTKVFRKRNNQGLHAL 249
           N VP  Y G   P      PN++ +L+RL    +PD  +   K  N+ + AL
Sbjct: 560 NDVPVSYSGSGGPTKSIRKPNLEEILRRLLSSLKPDTFQGAIKAINEKILAL 611


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,250,661
Number of Sequences: 28952
Number of extensions: 233434
Number of successful extensions: 517
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 510
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 517
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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