Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS306G01f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme... 56 2e-08
At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme... 56 2e-08
At5g28030.2 68418.m03377 cysteine synthase, putative / O-acetyls... 37 0.009
At5g28030.1 68418.m03376 cysteine synthase, putative / O-acetyls... 37 0.009
At3g22460.1 68416.m02839 cysteine synthase, putative / O-acetyls... 37 0.009
At4g14880.2 68417.m02286 cysteine synthase / O-acetylserine (thi... 36 0.017
At4g14880.1 68417.m02285 cysteine synthase / O-acetylserine (thi... 36 0.017
At5g28020.2 68418.m03375 cysteine synthase, putative / O-acetyls... 36 0.022
At5g28020.1 68418.m03374 cysteine synthase, putative / O-acetyls... 36 0.022
At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putativ... 32 0.20
At3g61440.1 68416.m06881 cysteine synthase, putative / O-acetyls... 30 0.82
At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetyls... 29 1.4
At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase f... 28 4.4
>At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 348
Score = 55.6 bits (128), Expect = 2e-08
Identities = 22/43 (51%), Positives = 33/43 (76%)
Frame = +2
Query: 392 NNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
N +++ IG+TPL+R+N + + G CE+ KCEF+NPGGS+KD
Sbjct: 42 NGLVDAIGNTPLIRINSLSEATG--CEILGKCEFLNPGGSVKD 82
>At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 421
Score = 55.6 bits (128), Expect = 2e-08
Identities = 22/43 (51%), Positives = 33/43 (76%)
Frame = +2
Query: 392 NNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
N +++ IG+TPL+R+N + + G CE+ KCEF+NPGGS+KD
Sbjct: 42 NGLVDAIGNTPLIRINSLSEATG--CEILGKCEFLNPGGSVKD 82
>At5g28030.2 68418.m03377 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase [Brassica juncea] GI:2245144; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 323
Score = 36.7 bits (81), Expect = 0.009
Identities = 20/45 (44%), Positives = 27/45 (60%)
Frame = +2
Query: 386 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
I N++ E IG+TP+V LN+I G + AK E + P SIKD
Sbjct: 7 IKNDVTELIGNTPMVYLNKIVD--GCVARIAAKLEMMEPCSSIKD 49
>At5g28030.1 68418.m03376 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase [Brassica juncea] GI:2245144; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 323
Score = 36.7 bits (81), Expect = 0.009
Identities = 20/45 (44%), Positives = 27/45 (60%)
Frame = +2
Query: 386 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
I N++ E IG+TP+V LN+I G + AK E + P SIKD
Sbjct: 7 IKNDVTELIGNTPMVYLNKIVD--GCVARIAAKLEMMEPCSSIKD 49
>At3g22460.1 68416.m02839 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative nearly identical over 185 amino
acids to SP|P47998 Cysteine synthase (EC 4.2.99.8)
(O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) {Arabidopsis thaliana}
Length = 188
Score = 36.7 bits (81), Expect = 0.009
Identities = 19/46 (41%), Positives = 28/46 (60%)
Frame = +2
Query: 383 RIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
+I ++ E IG+TPLV LN++ KD + AK E + P S+KD
Sbjct: 7 KIAKDVTELIGNTPLVYLNKVAKD--CVGHVAAKLEMMEPCSSVKD 50
>At4g14880.2 68417.m02286 cysteine synthase / O-acetylserine
(thiol)-lyase / O-acetylserine sulfhydrylase (OAS1)
nearly identical to SP|P47998 Cysteine synthase (EC
4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) {Arabidopsis thaliana}; identical to cDNA
O-acetylserine lyase (At.OAS.5-8) GI:6983573
Length = 322
Score = 35.9 bits (79), Expect = 0.017
Identities = 19/47 (40%), Positives = 28/47 (59%)
Frame = +2
Query: 380 NRIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
+RI ++ E IG+TPLV LN + + G + AK E + P S+KD
Sbjct: 3 SRIAKDVTELIGNTPLVYLNNVAE--GCVGRVAAKLEMMEPCSSVKD 47
>At4g14880.1 68417.m02285 cysteine synthase / O-acetylserine
(thiol)-lyase / O-acetylserine sulfhydrylase (OAS1)
nearly identical to SP|P47998 Cysteine synthase (EC
4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) {Arabidopsis thaliana}; identical to cDNA
O-acetylserine lyase (At.OAS.5-8) GI:6983573
Length = 322
Score = 35.9 bits (79), Expect = 0.017
Identities = 19/47 (40%), Positives = 28/47 (59%)
Frame = +2
Query: 380 NRIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
+RI ++ E IG+TPLV LN + + G + AK E + P S+KD
Sbjct: 3 SRIAKDVTELIGNTPLVYLNNVAE--GCVGRVAAKLEMMEPCSSVKD 47
>At5g28020.2 68418.m03375 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 323
Score = 35.5 bits (78), Expect = 0.022
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +2
Query: 386 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
I N+I E IG+TP+V LN + G + AK E + P S+KD
Sbjct: 7 IKNDITELIGNTPMVYLNNVVD--GCVARIAAKLEMMEPCSSVKD 49
>At5g28020.1 68418.m03374 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 323
Score = 35.5 bits (78), Expect = 0.022
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +2
Query: 386 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
I N+I E IG+TP+V LN + G + AK E + P S+KD
Sbjct: 7 IKNDITELIGNTPMVYLNNVVD--GCVARIAAKLEMMEPCSSVKD 49
>At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to SP|O22682 Probable
cysteine synthase, chloroplast precursor {Arabidopsis
thaliana}, similar to SP|P31300 Cysteine synthase,
chloroplast precursor {Capsicum annuum}
Length = 404
Score = 32.3 bits (70), Expect = 0.20
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +2
Query: 386 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
I ++ + IG TP+V LNR+ G ++ AK E + P S+KD
Sbjct: 99 IAEDVTQLIGSTPMVYLNRVTD--GCLADIAAKLESMEPCRSVKD 141
>At3g61440.1 68416.m06881 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to cysteine synthase
(EC 4.2.99.8) [Arabidopsis thaliana] GI:5824334;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 368
Score = 30.3 bits (65), Expect = 0.82
Identities = 18/37 (48%), Positives = 22/37 (59%)
Frame = +2
Query: 410 IGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
IG TPLV LN++ + G E + AK E P SIKD
Sbjct: 57 IGKTPLVFLNKVTE--GCEAYVAAKQEHFQPTCSIKD 91
>At3g04940.1 68416.m00536 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 324
Score = 29.5 bits (63), Expect = 1.4
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +2
Query: 377 RNRIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKD 520
R I ++ + IG+TP+V LN I G + AK E + P S+K+
Sbjct: 5 RCSIKDDATQLIGNTPMVYLNNIVD--GCVARIAAKLEMMEPCSSVKE 50
>At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to steroid 5alpha-reductase - Rattus
norvegicus, PIR:A34239 [SP|24008]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 268
Score = 27.9 bits (59), Expect = 4.4
Identities = 16/58 (27%), Positives = 29/58 (50%), Gaps = 5/58 (8%)
Frame = -2
Query: 502 GIHEFAFCKHFTFQAIVFWNSIESNKRCITDSF-----*YVIINSVASKTS*IGHFLD 344
G+ + C H+ F+ +VFW+ ++ + SF Y+I S A++T + F D
Sbjct: 198 GLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSYATRTWYLSKFDD 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,935,703
Number of Sequences: 28952
Number of extensions: 168416
Number of successful extensions: 327
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 319
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 325
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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