BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS306F08f (495 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase / 10-f... 101 2e-22 At2g12280.1 68415.m01325 ligase, putative identical to residues... 89 1e-18 At2g12200.1 68415.m01318 ligase, putative similar to 10-formylte... 52 2e-07 At3g07540.1 68416.m00900 formin homology 2 domain-containing pro... 30 0.74 At1g05070.1 68414.m00509 expressed protein 30 0.98 At3g17380.1 68416.m02221 meprin and TRAF homology domain-contain... 29 2.3 At3g56580.2 68416.m06292 zinc finger (C3HC4-type RING finger) fa... 28 3.0 At3g56580.1 68416.m06291 zinc finger (C3HC4-type RING finger) fa... 28 3.0 At1g04790.1 68414.m00475 zinc finger (C3HC4-type RING finger) fa... 28 3.0 At5g37380.2 68418.m04492 DNAJ heat shock N-terminal domain-conta... 28 4.0 At5g37380.1 68418.m04491 DNAJ heat shock N-terminal domain-conta... 28 4.0 At2g42810.1 68415.m05300 serine/threonine protein phosphatase, p... 28 4.0 At5g35830.1 68418.m04305 ankyrin repeat family protein contains ... 27 6.9 At5g15050.1 68418.m01764 glycosyltransferase family 14 protein /... 27 9.2 At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containi... 27 9.2 >At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase / 10-formyltetrahydrofolate synthetase (THFS) identical to 10-formyltetrahydrofolate synthetase (Arabidopsis thaliana) GI:5921663 Length = 634 Score = 101 bits (243), Expect = 2e-22 Identities = 42/84 (50%), Positives = 62/84 (73%) Frame = -2 Query: 491 IFTEKGYDKFPICMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKM 312 ++T++G+ PICM+KT S + D + KGAP+ F L I + S+GAGF+ P+VG +S M Sbjct: 550 MYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYPLVGTMSTM 609 Query: 311 PGLPTRPSIYDIDLNTKTGEIEGL 240 PGLPTRP Y+ID++T+TG++ GL Sbjct: 610 PGLPTRPCFYEIDIDTETGKVRGL 633 >At2g12280.1 68415.m01325 ligase, putative identical to residues 550 - 623 of 10-formyltetrahydrofolate synthetase (Formate--tetrahydrofolate ligase (EC 6.3.4.3)) from Arabidopsis thaliana GI:5921663 Length = 90 Score = 89.4 bits (212), Expect = 1e-18 Identities = 37/75 (49%), Positives = 54/75 (72%) Frame = -2 Query: 491 IFTEKGYDKFPICMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKM 312 ++T++G+ PICM+KT S + D + KGAP+ F L I + S+GAGF+ P+VG +S M Sbjct: 1 MYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYPLVGTMSTM 60 Query: 311 PGLPTRPSIYDIDLN 267 PGLPTRP Y+ID++ Sbjct: 61 PGLPTRPCFYEIDIS 75 >At2g12200.1 68415.m01318 ligase, putative similar to 10-formyltetrahydrofolate synthetase (Formate--tetrahydrofolate ligase (EC 6.3.4.3)) from Arabidopsis thaliana GI:5921663, Spinacia oleracea SP|P28723| Length = 63 Score = 52.4 bits (120), Expect = 2e-07 Identities = 22/63 (34%), Positives = 38/63 (60%) Frame = -2 Query: 491 IFTEKGYDKFPICMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKM 312 ++T++G+ PICM+KT S + D + K AP+ F L I + S+GAGF+ ++ + Sbjct: 1 MYTQQGFSNLPICMSKTQYSFSHDTSKKRAPSGFVLPIRDVRGSIGAGFIYTTKKTVNPL 60 Query: 311 PGL 303 G+ Sbjct: 61 AGM 63 >At3g07540.1 68416.m00900 formin homology 2 domain-containing protein / FH2 domain-containing protein contains formin homology 2 domain, Pfam:PF02181 Length = 841 Score = 30.3 bits (65), Expect = 0.74 Identities = 16/54 (29%), Positives = 27/54 (50%) Frame = -2 Query: 455 CMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKMPGLPTR 294 C +N+ ++ + +D L + + S G+GFV P +IS +P LP R Sbjct: 154 CPPPRNNNTQNKLSVAPSTSD-VLYLGNVVTSSGSGFVKPESPDISPLPPLPAR 206 >At1g05070.1 68414.m00509 expressed protein Length = 184 Score = 29.9 bits (64), Expect = 0.98 Identities = 11/43 (25%), Positives = 24/43 (55%) Frame = -3 Query: 313 CPDCPRDLASTTLTSTPKQGKSKAFSKCSRNSIILNLYIFSNY 185 CP CP + ++ + + PK+ + +F+ C+++ +N NY Sbjct: 44 CPSCPCECSTYSAVTIPKELSNASFADCAKHDPEVNEDTEKNY 86 >At3g17380.1 68416.m02221 meprin and TRAF homology domain-containing protein / MATH domain-containing protein similar to ubiquitin-specific protease 12 [Arabidopsis thaliana] GI:11993471; contains Pfam profile PF00917: MATH domain Length = 309 Score = 28.7 bits (61), Expect = 2.3 Identities = 14/50 (28%), Positives = 25/50 (50%) Frame = -2 Query: 233 VFTEFNNLEFIHIFELLNSLHTTARITHRYTKISPREA*YKIISIFYYTK 84 +F EF I IF+ L H ++T +++ S K +S+ Y+T+ Sbjct: 238 IFVEFT----IRIFDQLQGRHIAGKVTKWFSRSSSEHGWVKYVSMVYFTQ 283 >At3g56580.2 68416.m06292 zinc finger (C3HC4-type RING finger) family protein contains INTERPRO domain, IPR001841, RING finger Length = 320 Score = 28.3 bits (60), Expect = 3.0 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 3/51 (5%) Frame = -3 Query: 337 PWLGRSRRCPDCPRDLASTTLTSTPKQGKSKAFS---KCSRNSIILNLYIF 194 PWL + CP C ++L S +S+ + ++++ + R +I NL+ F Sbjct: 215 PWLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPF 265 >At3g56580.1 68416.m06291 zinc finger (C3HC4-type RING finger) family protein contains INTERPRO domain, IPR001841, RING finger Length = 320 Score = 28.3 bits (60), Expect = 3.0 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 3/51 (5%) Frame = -3 Query: 337 PWLGRSRRCPDCPRDLASTTLTSTPKQGKSKAFS---KCSRNSIILNLYIF 194 PWL + CP C ++L S +S+ + ++++ + R +I NL+ F Sbjct: 215 PWLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPF 265 >At1g04790.1 68414.m00475 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 634 Score = 28.3 bits (60), Expect = 3.0 Identities = 9/12 (75%), Positives = 10/12 (83%) Frame = -3 Query: 337 PWLGRSRRCPDC 302 PWLGRS+ CP C Sbjct: 618 PWLGRSKSCPVC 629 >At5g37380.2 68418.m04492 DNAJ heat shock N-terminal domain-containing protein similar to SP|Q9QYI4 DnaJ homolog subfamily B member 12 {Mus musculus}; contains Pfam profile PF00226: DnaJ domain Length = 431 Score = 27.9 bits (59), Expect = 4.0 Identities = 14/31 (45%), Positives = 16/31 (51%) Frame = -3 Query: 316 RCPDCPRDLASTTLTSTPKQGKSKAFSKCSR 224 RCP+C L S PK G S +S CSR Sbjct: 264 RCPNC---LQSYLAVEVPKPGISSRWSSCSR 291 >At5g37380.1 68418.m04491 DNAJ heat shock N-terminal domain-containing protein similar to SP|Q9QYI4 DnaJ homolog subfamily B member 12 {Mus musculus}; contains Pfam profile PF00226: DnaJ domain Length = 431 Score = 27.9 bits (59), Expect = 4.0 Identities = 14/31 (45%), Positives = 16/31 (51%) Frame = -3 Query: 316 RCPDCPRDLASTTLTSTPKQGKSKAFSKCSR 224 RCP+C L S PK G S +S CSR Sbjct: 264 RCPNC---LQSYLAVEVPKPGISSRWSSCSR 291 >At2g42810.1 68415.m05300 serine/threonine protein phosphatase, putative similar to SP|P53042 Serine/threonine protein phosphatase 5 (EC 3.1.3.16) (PP5) (Protein phosphatase T) (PPT) {Rattus norvegicus}; contains Pfam profiles PF00149: Ser/Thr protein phosphatase, PF00515: TPR Domain Length = 484 Score = 27.9 bits (59), Expect = 4.0 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = -1 Query: 486 HGKGIRQISDLHGQDFELLN 427 HGK I D+HGQ ++LLN Sbjct: 219 HGKHITVCGDVHGQFYDLLN 238 >At5g35830.1 68418.m04305 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 282 Score = 27.1 bits (57), Expect = 6.9 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = -2 Query: 230 FTEFNNLEFIHIFELLNSLHTTARITHRYTKISPRE 123 F EFN+ EF+++F + S R HR + RE Sbjct: 216 FREFNDEEFVNLFHAVISADIYVRSIHRCSSGDVRE 251 >At5g15050.1 68418.m01764 glycosyltransferase family 14 protein / core-2/I-branching enzyme family protein contains Pfam profile: PF02485 Core-2/I-Branching enzyme Length = 434 Score = 26.6 bits (56), Expect = 9.2 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = +3 Query: 393 VSGSSLDGGITG*GVRSLGHANRKFVVSLFRE 488 +SGSS DG + + +L H N ++VV L RE Sbjct: 92 ISGSSGDGQMLKRTLMALYHPNNQYVVHLDRE 123 >At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containing protein contains Pfam profile: PF00806 Pumilio-family RNA binding domains (aka PUM-HD, Pumilio homology domain) (8 copies at C-terminal half of protein) Length = 650 Score = 26.6 bits (56), Expect = 9.2 Identities = 14/38 (36%), Positives = 24/38 (63%) Frame = -3 Query: 262 KQGKSKAFSKCSRNSIILNLYIFSNY*IRYIQLHELRI 149 +Q + K ++ SRNS++L F NY ++++ ELRI Sbjct: 508 RQQREKLIAEISRNSLLLAQDPFGNYAVQFV--IELRI 543 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,720,091 Number of Sequences: 28952 Number of extensions: 220737 Number of successful extensions: 623 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 608 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 622 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 868578304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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