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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS306F08f
         (495 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase / 10-f...   101   2e-22
At2g12280.1 68415.m01325 ligase, putative identical  to residues...    89   1e-18
At2g12200.1 68415.m01318 ligase, putative similar to 10-formylte...    52   2e-07
At3g07540.1 68416.m00900 formin homology 2 domain-containing pro...    30   0.74 
At1g05070.1 68414.m00509 expressed protein                             30   0.98 
At3g17380.1 68416.m02221 meprin and TRAF homology domain-contain...    29   2.3  
At3g56580.2 68416.m06292 zinc finger (C3HC4-type RING finger) fa...    28   3.0  
At3g56580.1 68416.m06291 zinc finger (C3HC4-type RING finger) fa...    28   3.0  
At1g04790.1 68414.m00475 zinc finger (C3HC4-type RING finger) fa...    28   3.0  
At5g37380.2 68418.m04492 DNAJ heat shock N-terminal domain-conta...    28   4.0  
At5g37380.1 68418.m04491 DNAJ heat shock N-terminal domain-conta...    28   4.0  
At2g42810.1 68415.m05300 serine/threonine protein phosphatase, p...    28   4.0  
At5g35830.1 68418.m04305 ankyrin repeat family protein contains ...    27   6.9  
At5g15050.1 68418.m01764 glycosyltransferase family 14 protein /...    27   9.2  
At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containi...    27   9.2  

>At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase /
           10-formyltetrahydrofolate synthetase (THFS) identical to
           10-formyltetrahydrofolate synthetase (Arabidopsis
           thaliana) GI:5921663
          Length = 634

 Score =  101 bits (243), Expect = 2e-22
 Identities = 42/84 (50%), Positives = 62/84 (73%)
 Frame = -2

Query: 491 IFTEKGYDKFPICMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKM 312
           ++T++G+   PICM+KT  S + D + KGAP+ F L I  +  S+GAGF+ P+VG +S M
Sbjct: 550 MYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYPLVGTMSTM 609

Query: 311 PGLPTRPSIYDIDLNTKTGEIEGL 240
           PGLPTRP  Y+ID++T+TG++ GL
Sbjct: 610 PGLPTRPCFYEIDIDTETGKVRGL 633


>At2g12280.1 68415.m01325 ligase, putative identical  to residues
           550 - 623 of 10-formyltetrahydrofolate synthetase
           (Formate--tetrahydrofolate ligase (EC 6.3.4.3)) from
           Arabidopsis thaliana GI:5921663
          Length = 90

 Score = 89.4 bits (212), Expect = 1e-18
 Identities = 37/75 (49%), Positives = 54/75 (72%)
 Frame = -2

Query: 491 IFTEKGYDKFPICMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKM 312
           ++T++G+   PICM+KT  S + D + KGAP+ F L I  +  S+GAGF+ P+VG +S M
Sbjct: 1   MYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYPLVGTMSTM 60

Query: 311 PGLPTRPSIYDIDLN 267
           PGLPTRP  Y+ID++
Sbjct: 61  PGLPTRPCFYEIDIS 75


>At2g12200.1 68415.m01318 ligase, putative similar to
           10-formyltetrahydrofolate synthetase
           (Formate--tetrahydrofolate ligase (EC 6.3.4.3)) from
           Arabidopsis thaliana GI:5921663, Spinacia oleracea
           SP|P28723|
          Length = 63

 Score = 52.4 bits (120), Expect = 2e-07
 Identities = 22/63 (34%), Positives = 38/63 (60%)
 Frame = -2

Query: 491 IFTEKGYDKFPICMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKM 312
           ++T++G+   PICM+KT  S + D + K AP+ F L I  +  S+GAGF+      ++ +
Sbjct: 1   MYTQQGFSNLPICMSKTQYSFSHDTSKKRAPSGFVLPIRDVRGSIGAGFIYTTKKTVNPL 60

Query: 311 PGL 303
            G+
Sbjct: 61  AGM 63


>At3g07540.1 68416.m00900 formin homology 2 domain-containing
           protein / FH2 domain-containing protein contains formin
           homology 2 domain, Pfam:PF02181
          Length = 841

 Score = 30.3 bits (65), Expect = 0.74
 Identities = 16/54 (29%), Positives = 27/54 (50%)
 Frame = -2

Query: 455 CMAKTSNSLTGDPAIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKMPGLPTR 294
           C    +N+     ++  + +D  L +  +  S G+GFV P   +IS +P LP R
Sbjct: 154 CPPPRNNNTQNKLSVAPSTSD-VLYLGNVVTSSGSGFVKPESPDISPLPPLPAR 206


>At1g05070.1 68414.m00509 expressed protein
          Length = 184

 Score = 29.9 bits (64), Expect = 0.98
 Identities = 11/43 (25%), Positives = 24/43 (55%)
 Frame = -3

Query: 313 CPDCPRDLASTTLTSTPKQGKSKAFSKCSRNSIILNLYIFSNY 185
           CP CP + ++ +  + PK+  + +F+ C+++   +N     NY
Sbjct: 44  CPSCPCECSTYSAVTIPKELSNASFADCAKHDPEVNEDTEKNY 86


>At3g17380.1 68416.m02221 meprin and TRAF homology domain-containing
           protein / MATH domain-containing protein similar to
           ubiquitin-specific protease 12 [Arabidopsis thaliana]
           GI:11993471; contains Pfam profile PF00917: MATH domain
          Length = 309

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 14/50 (28%), Positives = 25/50 (50%)
 Frame = -2

Query: 233 VFTEFNNLEFIHIFELLNSLHTTARITHRYTKISPREA*YKIISIFYYTK 84
           +F EF     I IF+ L   H   ++T  +++ S      K +S+ Y+T+
Sbjct: 238 IFVEFT----IRIFDQLQGRHIAGKVTKWFSRSSSEHGWVKYVSMVYFTQ 283


>At3g56580.2 68416.m06292 zinc finger (C3HC4-type RING finger)
           family protein contains INTERPRO domain, IPR001841, RING
           finger
          Length = 320

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
 Frame = -3

Query: 337 PWLGRSRRCPDCPRDLASTTLTSTPKQGKSKAFS---KCSRNSIILNLYIF 194
           PWL +   CP C ++L S   +S+ +  ++++ +      R +I  NL+ F
Sbjct: 215 PWLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPF 265


>At3g56580.1 68416.m06291 zinc finger (C3HC4-type RING finger)
           family protein contains INTERPRO domain, IPR001841, RING
           finger
          Length = 320

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
 Frame = -3

Query: 337 PWLGRSRRCPDCPRDLASTTLTSTPKQGKSKAFS---KCSRNSIILNLYIF 194
           PWL +   CP C ++L S   +S+ +  ++++ +      R +I  NL+ F
Sbjct: 215 PWLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPF 265


>At1g04790.1 68414.m00475 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 634

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 9/12 (75%), Positives = 10/12 (83%)
 Frame = -3

Query: 337 PWLGRSRRCPDC 302
           PWLGRS+ CP C
Sbjct: 618 PWLGRSKSCPVC 629


>At5g37380.2 68418.m04492 DNAJ heat shock N-terminal
           domain-containing protein similar to SP|Q9QYI4 DnaJ
           homolog subfamily B member 12 {Mus musculus}; contains
           Pfam profile PF00226: DnaJ domain
          Length = 431

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 14/31 (45%), Positives = 16/31 (51%)
 Frame = -3

Query: 316 RCPDCPRDLASTTLTSTPKQGKSKAFSKCSR 224
           RCP+C   L S      PK G S  +S CSR
Sbjct: 264 RCPNC---LQSYLAVEVPKPGISSRWSSCSR 291


>At5g37380.1 68418.m04491 DNAJ heat shock N-terminal
           domain-containing protein similar to SP|Q9QYI4 DnaJ
           homolog subfamily B member 12 {Mus musculus}; contains
           Pfam profile PF00226: DnaJ domain
          Length = 431

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 14/31 (45%), Positives = 16/31 (51%)
 Frame = -3

Query: 316 RCPDCPRDLASTTLTSTPKQGKSKAFSKCSR 224
           RCP+C   L S      PK G S  +S CSR
Sbjct: 264 RCPNC---LQSYLAVEVPKPGISSRWSSCSR 291


>At2g42810.1 68415.m05300 serine/threonine protein phosphatase,
           putative similar to SP|P53042 Serine/threonine protein
           phosphatase 5 (EC 3.1.3.16) (PP5) (Protein phosphatase
           T) (PPT) {Rattus norvegicus}; contains Pfam profiles
           PF00149: Ser/Thr protein phosphatase, PF00515: TPR
           Domain
          Length = 484

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = -1

Query: 486 HGKGIRQISDLHGQDFELLN 427
           HGK I    D+HGQ ++LLN
Sbjct: 219 HGKHITVCGDVHGQFYDLLN 238


>At5g35830.1 68418.m04305 ankyrin repeat family protein contains
           ankyrin repeats, Pfam:PF00023
          Length = 282

 Score = 27.1 bits (57), Expect = 6.9
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = -2

Query: 230 FTEFNNLEFIHIFELLNSLHTTARITHRYTKISPRE 123
           F EFN+ EF+++F  + S     R  HR +    RE
Sbjct: 216 FREFNDEEFVNLFHAVISADIYVRSIHRCSSGDVRE 251


>At5g15050.1 68418.m01764 glycosyltransferase family 14 protein /
           core-2/I-branching enzyme family protein contains Pfam
           profile: PF02485 Core-2/I-Branching enzyme
          Length = 434

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 14/32 (43%), Positives = 20/32 (62%)
 Frame = +3

Query: 393 VSGSSLDGGITG*GVRSLGHANRKFVVSLFRE 488
           +SGSS DG +    + +L H N ++VV L RE
Sbjct: 92  ISGSSGDGQMLKRTLMALYHPNNQYVVHLDRE 123


>At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile: PF00806 Pumilio-family
           RNA binding domains (aka PUM-HD, Pumilio homology
           domain) (8 copies at C-terminal half of protein)
          Length = 650

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 14/38 (36%), Positives = 24/38 (63%)
 Frame = -3

Query: 262 KQGKSKAFSKCSRNSIILNLYIFSNY*IRYIQLHELRI 149
           +Q + K  ++ SRNS++L    F NY ++++   ELRI
Sbjct: 508 RQQREKLIAEISRNSLLLAQDPFGNYAVQFV--IELRI 543


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,720,091
Number of Sequences: 28952
Number of extensions: 220737
Number of successful extensions: 623
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 608
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 622
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 868578304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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