BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS306F02f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC630.11 |vps55||vacuolar sorting protein Vps55 |Schizosacchar... 49 4e-07 SPBC1271.02 |stt3||oligosaccharyltransferase subunit Stt3|Schizo... 30 0.18 SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 30 0.24 SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi... 26 3.9 SPBC21C3.18 |spo4||serine/threonine protein kinase Spo4|Schizosa... 25 5.2 SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex ... 25 6.8 SPAC4H3.04c |||UPF0103 family|Schizosaccharomyces pombe|chr 1|||... 25 6.8 SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 25 9.0 >SPAC630.11 |vps55||vacuolar sorting protein Vps55 |Schizosaccharomyces pombe|chr 1|||Manual Length = 122 Score = 49.2 bits (112), Expect = 4e-07 Identities = 29/116 (25%), Positives = 59/116 (50%), Gaps = 3/116 (2%) Frame = +3 Query: 183 MAGIKGLVSLAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMIARRHT---D 353 M+ ++ ++ L+ ++G VIL+CAL +K W+P ++ P+P ++ ++++ D Sbjct: 1 MSDLRKIIGLSSVLAVGFMLVILSCAL--FKNWYPL------LIAPLPNLLTKKYSTSHD 52 Query: 354 GAGGSNSACMETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521 + ++ F + + FALPIV G+I A ++ G I++L I Sbjct: 53 FLQEEDRNLLDFGRFTFGATICTGFALPIVFVNVGLIGTAAATMSCVGGSIIFLVI 108 >SPBC1271.02 |stt3||oligosaccharyltransferase subunit Stt3|Schizosaccharomyces pombe|chr 2|||Manual Length = 752 Score = 30.3 bits (65), Expect = 0.18 Identities = 13/27 (48%), Positives = 18/27 (66%) Frame = +3 Query: 399 ITMGFLVSSFALPIVLARAGVIFWGAC 479 I + L+S+FAL I ++G FWGAC Sbjct: 177 IAITLLMSTFALWIKAVKSGSSFWGAC 203 >SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 163 Score = 29.9 bits (64), Expect = 0.24 Identities = 18/78 (23%), Positives = 40/78 (51%), Gaps = 3/78 (3%) Frame = +3 Query: 297 VLFYILCPIPTMIARRHTDGAGGSNSACM--ETAVFITMGFLVSSFALPIVLARAGVIFW 470 +L YI+CP+ T+++ + S + + V + + F +S +P V +F+ Sbjct: 21 ILLYIVCPLETIVSILYWSIVSYDRSLLIPKDRPVPLPLNFDISVHLMPTVYTLIDYLFF 80 Query: 471 GACY-LTLAGNVIVYLTI 521 + L++ +++VYL+I Sbjct: 81 SPPFSLSIGPSLLVYLSI 98 >SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 25.8 bits (54), Expect = 3.9 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +2 Query: 83 KTKQIIYYLLAEVYFISGVFKQEENNY 163 KT +YY A+V F+SG F + ++Y Sbjct: 412 KTDSDLYYHRAQVRFVSGEFAEAISDY 438 >SPBC21C3.18 |spo4||serine/threonine protein kinase Spo4|Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 25.4 bits (53), Expect = 5.2 Identities = 22/97 (22%), Positives = 33/97 (34%), Gaps = 5/97 (5%) Frame = +2 Query: 194 KRFGVVGIRGFNWNDI---CHTSLCLTTIQTMVAILRCAILHSVP--NTYDDR*EAY*WC 358 KR G RGF ++ C + ++ IL C + H P DD Sbjct: 257 KRADRAGTRGFRAPEVLFRCQNQTSSIDVWSVGVILLCFLTHRYPFFRCEDDIDAIVELA 316 Query: 359 WRFKFRLYGNCSIYHNGIFSQLFCATDSSRQGWCDFL 469 F NC++ H I+S + W D + Sbjct: 317 HIFGRNGMSNCALLHGQIWSDNIPTLLDQKHNWLDLI 353 >SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex subunit Orp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +3 Query: 282 WPFFVVLFYILC-PIPTMIARRHTDGAGGSNSACMETAVFI 401 +PF+ VLFY C P+ A H +S+CM+ ++ Sbjct: 523 YPFYEVLFYDYCRPLNQAFASGHQRSV--IHSSCMDPEYYV 561 >SPAC4H3.04c |||UPF0103 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 25.0 bits (52), Expect = 6.8 Identities = 10/33 (30%), Positives = 17/33 (51%) Frame = +2 Query: 107 LLAEVYFISGVFKQEENNYT*NIKHNGWNKRFG 205 ++A F+S K E N++ + W +RFG Sbjct: 166 MMAAAKFLSQYIKDESNSFVISSDFCHWGRRFG 198 >SPBC4B4.04 |||translation initiation factor eIF2A |Schizosaccharomyces pombe|chr 2|||Manual Length = 576 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/25 (40%), Positives = 13/25 (52%) Frame = +2 Query: 380 YGNCSIYHNGIFSQLFCATDSSRQG 454 YG ++Y GI Q C D R+G Sbjct: 245 YGETNLYLLGITGQFDCRVDLDREG 269 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,176,009 Number of Sequences: 5004 Number of extensions: 43776 Number of successful extensions: 68 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 67 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 67 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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