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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS306F02f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g32410.2 68414.m04000 vacuolar protein sorting 55 family prot...    66   1e-11
At1g32410.1 68414.m03999 vacuolar protein sorting 55 family prot...    66   1e-11
At3g11530.2 68416.m01406 vacuolar protein sorting 55 family prot...    54   8e-08
At3g11530.1 68416.m01405 vacuolar protein sorting 55 family prot...    47   7e-06
At3g26780.1 68416.m03350 phosphoglycerate/bisphosphoglycerate mu...    28   3.3  
At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl...    28   3.3  
At5g47050.1 68418.m05798 expressed protein                             28   4.4  
At1g11670.1 68414.m01340 MATE efflux family protein similar to r...    28   4.4  
At3g14330.1 68416.m01812 pentatricopeptide (PPR) repeat-containi...    27   5.8  
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy...    27   7.7  

>At1g32410.2 68414.m04000 vacuolar protein sorting 55 family protein
           / VPS55 family protein contains Pfam domain PF04133:
           Vacuolar protein sorting 55
          Length = 140

 Score = 66.5 bits (155), Expect = 1e-11
 Identities = 38/106 (35%), Positives = 55/106 (51%), Gaps = 2/106 (1%)
 Frame = +3

Query: 210 LAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMI--ARRHTDGAGGSNSACM 383
           LA   S G+   ILACAL  +  WWP   V+ Y+L P+P +       T     S+++ +
Sbjct: 20  LAILVSTGIVLQILACAL--FNNWWPMLSVIMYVLLPMPLLFFGGSDSTSLFNESDNSWI 77

Query: 384 ETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521
             A F+T    V S A+P +L  AG+I WGA  L L+  V+  + I
Sbjct: 78  NAAKFLTGASAVGSVAIPSILKHAGLIGWGALALDLSSYVVFLVAI 123


>At1g32410.1 68414.m03999 vacuolar protein sorting 55 family protein
           / VPS55 family protein contains Pfam domain PF04133:
           Vacuolar protein sorting 55
          Length = 140

 Score = 66.5 bits (155), Expect = 1e-11
 Identities = 38/106 (35%), Positives = 55/106 (51%), Gaps = 2/106 (1%)
 Frame = +3

Query: 210 LAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMI--ARRHTDGAGGSNSACM 383
           LA   S G+   ILACAL  +  WWP   V+ Y+L P+P +       T     S+++ +
Sbjct: 20  LAILVSTGIVLQILACAL--FNNWWPMLSVIMYVLLPMPLLFFGGSDSTSLFNESDNSWI 77

Query: 384 ETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521
             A F+T    V S A+P +L  AG+I WGA  L L+  V+  + I
Sbjct: 78  NAAKFLTGASAVGSVAIPSILKHAGLIGWGALALDLSSYVVFLVAI 123


>At3g11530.2 68416.m01406 vacuolar protein sorting 55 family protein
           / VPS55 family protein contains Pfam domain PF04133:
           Vacuolar protein sorting 55
          Length = 126

 Score = 53.6 bits (123), Expect = 8e-08
 Identities = 35/112 (31%), Positives = 50/112 (44%), Gaps = 2/112 (1%)
 Frame = +3

Query: 192 IKGLVSLAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMIARRHTDG--AGG 365
           I+ L  LAF  S  +   ILACA+  Y  WWP    L Y++ P+P M     +       
Sbjct: 5   IEVLAGLAFMFSSSILLQILACAI--YGNWWPMLSALMYVVVPMPCMFFGGGSTQFLISR 62

Query: 366 SNSACMETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521
                ++ A F+T    V S A+PI+L  A +I  GA  +      I   T+
Sbjct: 63  DGGGWIDAAKFLTGASTVGSLAIPIILRHAQMIETGAMLIEFTSFFIFICTV 114


>At3g11530.1 68416.m01405 vacuolar protein sorting 55 family protein
           / VPS55 family protein contains Pfam domain PF04133:
           Vacuolar protein sorting 55
          Length = 113

 Score = 47.2 bits (107), Expect = 7e-06
 Identities = 29/94 (30%), Positives = 42/94 (44%), Gaps = 2/94 (2%)
 Frame = +3

Query: 246 ILACALPQYKLWWPFFVVLFYILCPIPTMIARRHTDG--AGGSNSACMETAVFITMGFLV 419
           ILACA+  Y  WWP    L Y++ P+P M     +            ++ A F+T    V
Sbjct: 10  ILACAI--YGNWWPMLSALMYVVVPMPCMFFGGGSTQFLISRDGGGWIDAAKFLTGASTV 67

Query: 420 SSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521
            S A+PI+L  A +I  GA  +      I   T+
Sbjct: 68  GSLAIPIILRHAQMIETGAMLIEFTSFFIFICTV 101


>At3g26780.1 68416.m03350 phosphoglycerate/bisphosphoglycerate
           mutase family protein similar to X4 protein GI:21386798,
           Y4 protein GI:21386800 from  [Silene dioica]; contains
           Pfam profiles PF00300: phosphoglycerate mutase family,
           PF01535: PPR repeat
          Length = 1053

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 13/40 (32%), Positives = 23/40 (57%)
 Frame = +2

Query: 128 ISGVFKQEENNYT*NIKHNGWNKRFGVVGIRGFNWNDICH 247
           +SGV ++  ++Y+    HN  ++R G V +   NW D+ H
Sbjct: 250 VSGVAEKLRSDYSSTTNHNETHERDGGVSLASTNW-DLLH 288


>At1g80460.1 68414.m09423 glycerol kinase, putative similar to
           glycerol kinase (ATP:glycerol 3-phosphotransferase,
           Glycerokinase, GK)[Mycobacterium tuberculosis]
           Swiss-Prot:O69664
          Length = 522

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 16/43 (37%), Positives = 22/43 (51%)
 Frame = +1

Query: 241 LSY*LVPYHNTNYGGHSSLCYSTFCAQYLR*SLGGILMVLEVQ 369
           L+Y L P   TNY    S+  +    Q+LR SLG I    E++
Sbjct: 299 LAYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIE 341


>At5g47050.1 68418.m05798 expressed protein
          Length = 300

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = -3

Query: 324 VLGTECRIAQRRMATIVCIVVRH 256
           ++G  CRI +RR AT++ +  RH
Sbjct: 248 MIGPGCRICRRRSATVLALPCRH 270


>At1g11670.1 68414.m01340 MATE efflux family protein similar to
           ripening regulated protein DDTFR18 [Lycopersicon
           esculentum] GI:12231296; contains Pfam profile PF01554:
           Uncharacterized membrane protein family; EST gb|W43487
           comes from this gene
          Length = 503

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +3

Query: 354 GAGGSNSACMETAVFITMGFLVSSFALPIVLARAGVI 464
           GAG   SA   TAV   + FL+S F   ++L+   VI
Sbjct: 343 GAGNPRSAAFSTAVTTGVSFLLSLFEAIVILSWRHVI 379


>At3g14330.1 68416.m01812 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 710

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
 Frame = +2

Query: 65  LIKVKGKTKQIIYYLLAEVYFISGVFKQEENNYT*NIKHN--GWNKRFGVV 211
           ++K K K  Q++Y +L ++Y  SG+F      +    + N   WN    V+
Sbjct: 262 IVKRKEKVDQVVYNVLLKLYMESGLFDDARKVFDGMSERNVVTWNSLISVL 312


>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 455

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = -2

Query: 427 KELTKNPIVINTAVSIQAEFEP 362
           KE    P++I+ A  ++AEFEP
Sbjct: 175 KERVNAPVIISLAKGVEAEFEP 196


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,219,890
Number of Sequences: 28952
Number of extensions: 226318
Number of successful extensions: 461
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 448
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 454
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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