BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS306F02f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g32410.2 68414.m04000 vacuolar protein sorting 55 family prot... 66 1e-11 At1g32410.1 68414.m03999 vacuolar protein sorting 55 family prot... 66 1e-11 At3g11530.2 68416.m01406 vacuolar protein sorting 55 family prot... 54 8e-08 At3g11530.1 68416.m01405 vacuolar protein sorting 55 family prot... 47 7e-06 At3g26780.1 68416.m03350 phosphoglycerate/bisphosphoglycerate mu... 28 3.3 At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl... 28 3.3 At5g47050.1 68418.m05798 expressed protein 28 4.4 At1g11670.1 68414.m01340 MATE efflux family protein similar to r... 28 4.4 At3g14330.1 68416.m01812 pentatricopeptide (PPR) repeat-containi... 27 5.8 At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy... 27 7.7 >At1g32410.2 68414.m04000 vacuolar protein sorting 55 family protein / VPS55 family protein contains Pfam domain PF04133: Vacuolar protein sorting 55 Length = 140 Score = 66.5 bits (155), Expect = 1e-11 Identities = 38/106 (35%), Positives = 55/106 (51%), Gaps = 2/106 (1%) Frame = +3 Query: 210 LAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMI--ARRHTDGAGGSNSACM 383 LA S G+ ILACAL + WWP V+ Y+L P+P + T S+++ + Sbjct: 20 LAILVSTGIVLQILACAL--FNNWWPMLSVIMYVLLPMPLLFFGGSDSTSLFNESDNSWI 77 Query: 384 ETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521 A F+T V S A+P +L AG+I WGA L L+ V+ + I Sbjct: 78 NAAKFLTGASAVGSVAIPSILKHAGLIGWGALALDLSSYVVFLVAI 123 >At1g32410.1 68414.m03999 vacuolar protein sorting 55 family protein / VPS55 family protein contains Pfam domain PF04133: Vacuolar protein sorting 55 Length = 140 Score = 66.5 bits (155), Expect = 1e-11 Identities = 38/106 (35%), Positives = 55/106 (51%), Gaps = 2/106 (1%) Frame = +3 Query: 210 LAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMI--ARRHTDGAGGSNSACM 383 LA S G+ ILACAL + WWP V+ Y+L P+P + T S+++ + Sbjct: 20 LAILVSTGIVLQILACAL--FNNWWPMLSVIMYVLLPMPLLFFGGSDSTSLFNESDNSWI 77 Query: 384 ETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521 A F+T V S A+P +L AG+I WGA L L+ V+ + I Sbjct: 78 NAAKFLTGASAVGSVAIPSILKHAGLIGWGALALDLSSYVVFLVAI 123 >At3g11530.2 68416.m01406 vacuolar protein sorting 55 family protein / VPS55 family protein contains Pfam domain PF04133: Vacuolar protein sorting 55 Length = 126 Score = 53.6 bits (123), Expect = 8e-08 Identities = 35/112 (31%), Positives = 50/112 (44%), Gaps = 2/112 (1%) Frame = +3 Query: 192 IKGLVSLAFAGSIGMTFVILACALPQYKLWWPFFVVLFYILCPIPTMIARRHTDG--AGG 365 I+ L LAF S + ILACA+ Y WWP L Y++ P+P M + Sbjct: 5 IEVLAGLAFMFSSSILLQILACAI--YGNWWPMLSALMYVVVPMPCMFFGGGSTQFLISR 62 Query: 366 SNSACMETAVFITMGFLVSSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521 ++ A F+T V S A+PI+L A +I GA + I T+ Sbjct: 63 DGGGWIDAAKFLTGASTVGSLAIPIILRHAQMIETGAMLIEFTSFFIFICTV 114 >At3g11530.1 68416.m01405 vacuolar protein sorting 55 family protein / VPS55 family protein contains Pfam domain PF04133: Vacuolar protein sorting 55 Length = 113 Score = 47.2 bits (107), Expect = 7e-06 Identities = 29/94 (30%), Positives = 42/94 (44%), Gaps = 2/94 (2%) Frame = +3 Query: 246 ILACALPQYKLWWPFFVVLFYILCPIPTMIARRHTDG--AGGSNSACMETAVFITMGFLV 419 ILACA+ Y WWP L Y++ P+P M + ++ A F+T V Sbjct: 10 ILACAI--YGNWWPMLSALMYVVVPMPCMFFGGGSTQFLISRDGGGWIDAAKFLTGASTV 67 Query: 420 SSFALPIVLARAGVIFWGACYLTLAGNVIVYLTI 521 S A+PI+L A +I GA + I T+ Sbjct: 68 GSLAIPIILRHAQMIETGAMLIEFTSFFIFICTV 101 >At3g26780.1 68416.m03350 phosphoglycerate/bisphosphoglycerate mutase family protein similar to X4 protein GI:21386798, Y4 protein GI:21386800 from [Silene dioica]; contains Pfam profiles PF00300: phosphoglycerate mutase family, PF01535: PPR repeat Length = 1053 Score = 28.3 bits (60), Expect = 3.3 Identities = 13/40 (32%), Positives = 23/40 (57%) Frame = +2 Query: 128 ISGVFKQEENNYT*NIKHNGWNKRFGVVGIRGFNWNDICH 247 +SGV ++ ++Y+ HN ++R G V + NW D+ H Sbjct: 250 VSGVAEKLRSDYSSTTNHNETHERDGGVSLASTNW-DLLH 288 >At1g80460.1 68414.m09423 glycerol kinase, putative similar to glycerol kinase (ATP:glycerol 3-phosphotransferase, Glycerokinase, GK)[Mycobacterium tuberculosis] Swiss-Prot:O69664 Length = 522 Score = 28.3 bits (60), Expect = 3.3 Identities = 16/43 (37%), Positives = 22/43 (51%) Frame = +1 Query: 241 LSY*LVPYHNTNYGGHSSLCYSTFCAQYLR*SLGGILMVLEVQ 369 L+Y L P TNY S+ + Q+LR SLG I E++ Sbjct: 299 LAYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIE 341 >At5g47050.1 68418.m05798 expressed protein Length = 300 Score = 27.9 bits (59), Expect = 4.4 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = -3 Query: 324 VLGTECRIAQRRMATIVCIVVRH 256 ++G CRI +RR AT++ + RH Sbjct: 248 MIGPGCRICRRRSATVLALPCRH 270 >At1g11670.1 68414.m01340 MATE efflux family protein similar to ripening regulated protein DDTFR18 [Lycopersicon esculentum] GI:12231296; contains Pfam profile PF01554: Uncharacterized membrane protein family; EST gb|W43487 comes from this gene Length = 503 Score = 27.9 bits (59), Expect = 4.4 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = +3 Query: 354 GAGGSNSACMETAVFITMGFLVSSFALPIVLARAGVI 464 GAG SA TAV + FL+S F ++L+ VI Sbjct: 343 GAGNPRSAAFSTAVTTGVSFLLSLFEAIVILSWRHVI 379 >At3g14330.1 68416.m01812 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 710 Score = 27.5 bits (58), Expect = 5.8 Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = +2 Query: 65 LIKVKGKTKQIIYYLLAEVYFISGVFKQEENNYT*NIKHN--GWNKRFGVV 211 ++K K K Q++Y +L ++Y SG+F + + N WN V+ Sbjct: 262 IVKRKEKVDQVVYNVLLKLYMESGLFDDARKVFDGMSERNVVTWNSLISVL 312 >At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehydrogenase family protein weak similarity to SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 455 Score = 27.1 bits (57), Expect = 7.7 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = -2 Query: 427 KELTKNPIVINTAVSIQAEFEP 362 KE P++I+ A ++AEFEP Sbjct: 175 KERVNAPVIISLAKGVEAEFEP 196 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,219,890 Number of Sequences: 28952 Number of extensions: 226318 Number of successful extensions: 461 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 448 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 454 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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