Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS306F01f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g07560.1 68416.m00903 glycine-rich protein 33 0.088
At4g33490.1 68417.m04756 nucellin protein, putative similar to n... 32 0.20
At1g23780.1 68414.m03000 F-box family protein contains Pfam PF00... 31 0.36
At5g60640.2 68418.m07611 thioredoxin family protein similar to p... 30 0.82
At5g60640.1 68418.m07610 thioredoxin family protein similar to p... 30 0.82
At1g26250.1 68414.m03202 proline-rich extensin, putative similar... 30 0.82
At5g66400.1 68418.m08374 dehydrin (RAB18) nearly identical to SP... 30 1.1
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa... 29 2.5
At5g14610.1 68418.m01713 DEAD box RNA helicase, putative similar... 28 3.3
At2g25970.1 68415.m03117 KH domain-containing protein 28 3.3
At1g26110.1 68414.m03186 expressed protein 28 3.3
At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl... 28 4.4
At5g61660.1 68418.m07736 glycine-rich protein 27 5.8
At4g08380.1 68417.m01384 proline-rich extensin-like family prote... 27 5.8
At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) i... 27 5.8
At1g11650.2 68414.m01337 RNA-binding protein 45 (RBP45), putativ... 27 5.8
At4g39400.1 68417.m05577 brassinosteroid insensitive 1 (BRI1) id... 27 7.7
At3g43960.1 68416.m04706 cysteine proteinase, putative contains ... 27 7.7
At3g22800.1 68416.m02874 leucine-rich repeat family protein / ex... 27 7.7
At2g42510.1 68415.m05261 spliceosome protein-related contains Pf... 27 7.7
At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containi... 27 7.7
At1g26240.1 68414.m03201 proline-rich extensin-like family prote... 27 7.7
At1g12040.1 68414.m01390 leucine-rich repeat family protein / ex... 27 7.7
>At3g07560.1 68416.m00903 glycine-rich protein
Length = 304
Score = 33.5 bits (73), Expect = 0.088
Identities = 25/89 (28%), Positives = 36/89 (40%), Gaps = 9/89 (10%)
Frame = +1
Query: 109 PSGKRMSTAFIEQLAYPNPGDSWRSE--WTPQLQKYMQHYTPDSGRGSQTYGPTNNGYG- 279
P + + A + L+ P P W + + Y + SG GS TYG GYG
Sbjct: 53 PVNRPNTAANMNSLSRPVPARPWEQQNYGSTMGGGYGSNLGMTSGYGSGTYGSALGGYGS 112
Query: 280 ------FSASQIYGQTGGGSQIYGYTGEY 348
+ S +Y GG +YG +G Y
Sbjct: 113 SYGGGMYGGSSMYRGGYGGGGLYGSSGMY 141
>At4g33490.1 68417.m04756 nucellin protein, putative similar to
nucellin GI:2290202 from [Hordeum vulgare]
Length = 425
Score = 32.3 bits (70), Expect = 0.20
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 169 DSWRSEWTPQLQKYMQHYTPDSGRGSQTYGPTNNG 273
DS R WTP ++Y +HY+P G G +G G
Sbjct: 234 DSSRVSWTPMSREYSKHYSPAMG-GELLFGGRTTG 267
>At1g23780.1 68414.m03000 F-box family protein contains Pfam
PF00646: F-box domain; similar to SP:Q9Y3I1 F-box only
protein 7 {Homo sapiens}; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 475
Score = 31.5 bits (68), Expect = 0.36
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +1
Query: 34 RDLDKSHMGGFDDMHQLKERDLPYLPSGKRMSTAFIEQLAYPNPGDSWRS 183
R+ K + D +H L+ R P +PS +S ++L P+P D+ RS
Sbjct: 11 RETLKLELADADTLHDLRRRINPTVPSSVHLSLNRKDELITPSPEDTLRS 60
>At5g60640.2 68418.m07611 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 536
Score = 30.3 bits (65), Expect = 0.82
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +1
Query: 265 NNGYGFSASQIYGQTGGGSQIYGYTG-EYPRAHY 363
N YG ++ +G +G G ++ GYTG E P+ ++
Sbjct: 371 NEDYGKPVAEYFGVSGNGPKLIGYTGNEDPKKYF 404
>At5g60640.1 68418.m07610 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 597
Score = 30.3 bits (65), Expect = 0.82
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +1
Query: 265 NNGYGFSASQIYGQTGGGSQIYGYTG-EYPRAHY 363
N YG ++ +G +G G ++ GYTG E P+ ++
Sbjct: 371 NEDYGKPVAEYFGVSGNGPKLIGYTGNEDPKKYF 404
>At1g26250.1 68414.m03202 proline-rich extensin, putative similar to
extensin gi|1165322|gb|AAB53156; contains proline-rich
extensin domains, INTERPRO:IPR002965
Length = 443
Score = 30.3 bits (65), Expect = 0.82
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -1
Query: 347 YSPV*PYIWDPPPVCPYIWEALNP*PLFVGP 255
YSP+ PY+++ PP PY++ + +P P P
Sbjct: 34 YSPLPPYVYNSPP--PYVYNSPSPPPYVYKP 62
Score = 29.5 bits (63), Expect = 1.4
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 341 PV*PYIWDPPPVCPYIWEALNP*P 270
P PY++ PPP PY++++ P P
Sbjct: 161 PPPPYVYSPPPPPPYVYQSPPPPP 184
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = -1
Query: 341 PV*PYIWDPPPVCPYIWEALNP*PLFVGP 255
P PY++ PP PY++++ P P P
Sbjct: 141 PPPPYVYSSPPPPPYVYKSPPPPPYVYSP 169
>At5g66400.1 68418.m08374 dehydrin (RAB18) nearly identical to
SP|P30185 Dehydrin Rab18 {Arabidopsis thaliana}
Length = 186
Score = 29.9 bits (64), Expect = 1.1
Identities = 19/42 (45%), Positives = 23/42 (54%)
Frame = +1
Query: 235 GRGSQTYGPTNNGYGFSASQIYGQTGGGSQIYGYTGEYPRAH 360
G G Q YG GYG + Q YG TG G++ +G TG R H
Sbjct: 51 GTGGQGYGSGGQGYG-TGGQGYG-TGTGTEGFG-TGGGARHH 89
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 235 GRGSQTYGPTNNGYGFSASQIYGQTGGGSQIYG 333
G G Q YG GYG TGGG++ +G
Sbjct: 58 GSGGQGYGTGGQGYGTGTGTEGFGTGGGARHHG 90
>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
profiles: PF04096 nucleoporin autopeptidase, PF03093
nucleoporin FG repeat family
Length = 1041
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +1
Query: 223 TPDSGRGSQTYGPTNNGYGFSASQIYGQTGGGSQI 327
TP G+Q PT G GF S GQ GG +
Sbjct: 315 TPSPFGGAQASTPTFGGSGFGQSTFGGQQGGSRAV 349
>At5g14610.1 68418.m01713 DEAD box RNA helicase, putative similar to
RNA helicase DRH1 [Arabidopsis thaliana] GI:3149952;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF00397:
WW domain
Length = 713
Score = 28.3 bits (60), Expect = 3.3
Identities = 16/49 (32%), Positives = 22/49 (44%)
Frame = +1
Query: 199 LQKYMQHYTPDSGRGSQTYGPTNNGYGFSASQIYGQTGGGSQIYGYTGE 345
+ K+ + TP SG G G ++GYG YG G YG G+
Sbjct: 614 MNKFRRWGTPSSGGGGGRGGYGDSGYGGRGESGYGSRGDSG--YGGRGD 660
>At2g25970.1 68415.m03117 KH domain-containing protein
Length = 632
Score = 28.3 bits (60), Expect = 3.3
Identities = 24/68 (35%), Positives = 29/68 (42%), Gaps = 3/68 (4%)
Frame = +1
Query: 142 EQLAYPNPGDSWRSEWTPQLQKYMQHYTPDSGRGSQTYGPTNNGYG-FSASQI--YGQTG 312
+Q P+ G S Y QH SG G G +GYG ++ASQ YGQ
Sbjct: 396 QQSQQPSSGGSSAPPTDTTGYNYYQHA---SGYGQAGQGYQQDGYGAYNASQQSGYGQAA 452
Query: 313 GGSQIYGY 336
G Q GY
Sbjct: 453 GYDQQGGY 460
>At1g26110.1 68414.m03186 expressed protein
Length = 611
Score = 28.3 bits (60), Expect = 3.3
Identities = 16/38 (42%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
Frame = +1
Query: 235 GRGS--QTYGPTNNGYGFSASQIYGQTGGGSQIYGYTG 342
GRG + G + GYG YG GGG YGY G
Sbjct: 562 GRGGYGRNNGYSRGGYGGRGYGGYGGRGGGGGGYGYGG 599
>At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly
identical to acyl-activating enzyme 17 [Arabidopsis
thaliana] GI:29893266; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-activating enzyme 17 (At5g23050) GI:29893265
Length = 721
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 262 TNNGYGFSASQIYGQTGGGSQIYGYTGEYPRAHY 363
+ +GY +S + +G TG S I Y RAHY
Sbjct: 457 STSGYDWSRIRCFGSTGEASNIDEYLWLMGRAHY 490
>At5g61660.1 68418.m07736 glycine-rich protein
Length = 134
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +1
Query: 235 GRGSQTYGPTNNGYGFSASQIYGQTGGGSQIYGYTGE 345
G GS G GYG+ + G++GGG G+ GE
Sbjct: 88 GYGSGGGGARGGGYGYGSGN--GRSGGGGGGGGFNGE 122
>At4g08380.1 68417.m01384 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 437
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -1
Query: 347 YSPV*PYIWDPPPVCPYIWE 288
YSP PY + PPP CP +++
Sbjct: 382 YSPP-PYAYSPPPPCPDVYK 400
>At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE)
identical to C2H2 zinc-finger protein SERRATE
GI:14486602 from [Arabidopsis thaliana]
Length = 720
Score = 27.5 bits (58), Expect = 5.8
Identities = 17/55 (30%), Positives = 24/55 (43%), Gaps = 2/55 (3%)
Frame = +1
Query: 229 DSGRGSQT--YGPTNNGYGFSASQIYGQTGGGSQIYGYTGEYPRAHYPRRRSDYD 387
D RGS YGP + +G+ Y + GG GY E P + R D++
Sbjct: 123 DGRRGSPRGGYGPPDRRFGYDHGGGYDREMGGRP--GYGDERPHGRFMGRYQDWE 175
>At1g11650.2 68414.m01337 RNA-binding protein 45 (RBP45), putative
similar to gb|U90212 DNA binding protein ACBF from
Nicotiana tabacum and contains 3 PF|00076 RNA
recognition motif domains. ESTs gb|T44278, gb|R65195,
gb|N65904, gb|H37499, gb|R90487, gb|N95952, gb|T44278,
gb|Z20166, gb|N96891, gb|W43137, gb|F15504, gb|F1
Length = 405
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +1
Query: 259 PTNNGYGFSASQIYGQTGGGSQIYGYT-GEYPRAHY 363
P+N G + YG G G + YGYT + P A+Y
Sbjct: 334 PSNKQSGDPSQFYYGGYGQGQEQYGYTMPQDPNAYY 369
>At4g39400.1 68417.m05577 brassinosteroid insensitive 1 (BRI1)
identical to GI:2392895
Length = 1196
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/69 (23%), Positives = 34/69 (49%)
Frame = +2
Query: 119 SVCQLLSLSN*HTRTLVTLGDRSGRRNYRNICSIIRQIRVGDHKLMVQRTMVMDSVLPKY 298
++ +L+ +S R + + G+ +RN+ ++ +VGD +L+V M S+
Sbjct: 909 AIKKLIHVSGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEFMKYGSLEDVL 968
Query: 299 MDRQEADPK 325
D ++A K
Sbjct: 969 HDPKKAGVK 977
>At3g43960.1 68416.m04706 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 376
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +1
Query: 190 TPQLQKYMQHYTPDSGRGSQTYGPTNNGYGFSASQIYGQTGG--GSQIYGYTGE 345
T +L + D RG+ +G G + A + + GG ++YGYTGE
Sbjct: 169 TGELVSLSEQELIDCDRGNDNFGCAGGGAVW-AFEFIKENGGIVSDEVYGYTGE 221
>At3g22800.1 68416.m02874 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycsimilar to extensin-like protein [Lycopersicon
esculentum] gi|5917664|gb|AAD55979; contains
leucine-rich repeats, Pfam:PF00560; contains proline
rich extensin domains, INTERPRO:IPR002965
Length = 470
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 341 PV*PYIWDPPPVCPYIWEALNP*P 270
P PY++ PPP PY++ P P
Sbjct: 428 PPPPYVYPPPPSPPYVYPPPPPSP 451
>At2g42510.1 68415.m05261 spliceosome protein-related contains Pfam
domain, PF04938: Survival motor neuron (SMN) interacting
protein 1 (SIP1)
Length = 646
Score = 27.1 bits (57), Expect = 7.7
Identities = 7/21 (33%), Positives = 16/21 (76%)
Frame = +3
Query: 273 LWIQCFPNIWTDRRRIPNIRL 335
+W+ CF ++ + +RIPN+++
Sbjct: 461 IWLSCFHHLRWEAKRIPNVKV 481
>At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile: PF00806 Pumilio-family
RNA binding domains (aka PUM-HD, Pumilio homology
domain) (8 copies at C-terminal half of protein)
Length = 650
Score = 27.1 bits (57), Expect = 7.7
Identities = 22/83 (26%), Positives = 38/83 (45%), Gaps = 5/83 (6%)
Frame = +1
Query: 4 APIPLSEEMLRDLDKSHMGGFDDM---HQLKERDLPYLPSGKRMSTAFIEQLAYPNPGDS 174
+P+ +D +KSH+ G DD+ L ++ ++ A E L P P S
Sbjct: 84 SPVSFDSLFFKDHEKSHVNGTDDLGLCEDLYRMNIKEDVEEDQIRYARTETLKDPLP-KS 142
Query: 175 WRSEWTPQ-LQKYM-QHYTPDSG 237
+++TP L + +HY P +G
Sbjct: 143 DHTDFTPDPLYNFSPKHYEPSNG 165
>At1g26240.1 68414.m03201 proline-rich extensin-like family protein
similar to hydroxyproline-rich glycoprotein precursor
gi|727264|gb|AAA87902; contains proline-rich extensin
domains, INTERPRO:IPR002965
Length = 478
Score = 27.1 bits (57), Expect = 7.7
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -1
Query: 341 PV*PYIWDPPPVCPYIWEALNP*P 270
P PY++ PP PY++++ +P P
Sbjct: 431 PPPPYVYSSPPPPPYVYKSPSPPP 454
>At1g12040.1 68414.m01390 leucine-rich repeat family protein /
extensin family protein (LRX1) similar to extensin-like
protein [Lycopersicon esculentum]
gi|5917664|gb|AAD55979; contains leucine-rich repeats,
Pfam:PF00560; contains proline rich extensin domains,
INTERPRO:IPR002965
Length = 744
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -1
Query: 344 SPV*PYIWDPPPVCPYIWEALNP*P 270
SP PY++ PP PY++ + P P
Sbjct: 474 SPPPPYVYSSPPPPPYVYSSPPPPP 498
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,165,970
Number of Sequences: 28952
Number of extensions: 247478
Number of successful extensions: 887
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 752
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 884
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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