Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS306E10f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g24280.1 68415.m02901 serine carboxypeptidase S28 family prot... 136 1e-32
At5g65760.1 68418.m08275 serine carboxypeptidase S28 family prot... 126 7e-30
At5g22860.2 68418.m02673 serine carboxypeptidase S28 family prot... 105 1e-23
At5g22860.1 68418.m02672 serine carboxypeptidase S28 family prot... 105 1e-23
At4g36195.1 68417.m05150 serine carboxypeptidase S28 family prot... 79 1e-15
At4g36190.1 68417.m05149 serine carboxypeptidase S28 family prot... 78 3e-15
At2g18080.1 68415.m02102 serine carboxypeptidase S28 family prot... 42 3e-04
At3g28680.1 68416.m03579 prolylcarboxypeptidase-related weak sim... 38 0.005
At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family prote... 32 0.27
At3g13000.2 68416.m01620 expressed protein contains Pfam profile... 31 0.36
At3g13000.1 68416.m01619 expressed protein contains Pfam profile... 31 0.36
At1g26360.1 68414.m03216 hydrolase, alpha/beta fold family prote... 29 1.9
At5g24260.1 68418.m02854 prolyl oligopeptidase family protein si... 29 2.5
At2g18360.1 68415.m02139 hydrolase, alpha/beta fold family prote... 28 3.3
At4g15370.1 68417.m02349 pentacyclic triterpene synthase, putati... 28 4.4
At1g17440.2 68414.m02133 transcription initiation factor IID (TF... 28 4.4
At1g17440.1 68414.m02132 transcription initiation factor IID (TF... 28 4.4
At2g14260.2 68415.m01594 proline iminopeptidase identical to GP:... 27 5.8
At2g14260.1 68415.m01595 proline iminopeptidase identical to GP:... 27 5.8
At4g24480.1 68417.m03509 serine/threonine protein kinase, putati... 27 7.7
At1g67510.1 68414.m07690 leucine-rich repeat family protein cont... 27 7.7
>At2g24280.1 68415.m02901 serine carboxypeptidase S28 family protein
contains Pfam profile: PF05577 Serine carboxypeptidase
S28
Length = 494
Score = 136 bits (328), Expect = 1e-32
Identities = 66/115 (57%), Positives = 85/115 (73%), Gaps = 1/115 (0%)
Frame = +3
Query: 150 GQIEAFANYTGFMWEIAAEYQAKIVFAEHRYYGESKPFGNKSLEK-EYIGYLTSAQALAD 326
G I+ FA+ TGFM +IA +++A +VF EHR+YGES PFG KS + E +GYL S QALAD
Sbjct: 93 GDIDWFASNTGFMLDIAPKFRALLVFIEHRFYGESTPFGKKSHKSAETLGYLNSQQALAD 152
Query: 327 YADLINYLQKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMF 491
YA LI L+++ PV+ FGGSYGGMLAA+ R+KYPH+ GA+A+SAPI F
Sbjct: 153 YAILIRSLKQNLSSEASPVVVFGGSYGGMLAAWFRLKYPHITIGALASSAPILHF 207
>At5g65760.1 68418.m08275 serine carboxypeptidase S28 family protein
similar to SP|P42785 Lysosomal Pro-X carboxypeptidase
precursor (EC 3.4.16.2) (Prolylcarboxypeptidase) (PRCP)
(Proline carboxypeptidase) {Homo sapiens}; contains Pfam
profile PF05577: Serine carboxypeptidase S28
Length = 515
Score = 126 bits (305), Expect = 7e-30
Identities = 61/119 (51%), Positives = 85/119 (71%), Gaps = 5/119 (4%)
Frame = +3
Query: 150 GQIEAFANYTGFMWEIAAEYQAKIVFAEHRYYGESKPFGNKSLEKEY-----IGYLTSAQ 314
G IE FA +GF+W+IA ++ A +VF EHRYYGES P+G++ E+ Y + YLT+ Q
Sbjct: 106 GDIEWFATNSGFIWDIAPKFGALLVFPEHRYYGESMPYGSR--EEAYKNATTLSYLTTEQ 163
Query: 315 ALADYADLINYLQKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMF 491
ALAD+A + L+++ PV+ FGGSYGGMLAA++R+KYPH+ GA+A+SAPI F
Sbjct: 164 ALADFAVFVTDLKRNLSAEACPVVLFGGSYGGMLAAWMRLKYPHIAIGALASSAPILQF 222
>At5g22860.2 68418.m02673 serine carboxypeptidase S28 family protein
contains Pfam profile: PF05577 serine carboxypeptidase
S28
Length = 439
Score = 105 bits (253), Expect = 1e-23
Identities = 50/107 (46%), Positives = 74/107 (69%), Gaps = 3/107 (2%)
Frame = +3
Query: 180 GFMWEIAAEYQAKIVFAEHRYYGESKPFGN--KSLEK-EYIGYLTSAQALADYADLINYL 350
GF+ + A +V+ EHRYYGE+ PFG+ ++L+ +GYL +AQALADYA ++ ++
Sbjct: 114 GFLRDNGPRLNALLVYIEHRYYGETMPFGSAEEALKNASTLGYLNAAQALADYAAILLHV 173
Query: 351 QKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMF 491
++ P+I GGSYGGMLAA+ R+KYPH+ GA+A+SAP+ F
Sbjct: 174 KEKYSTNHSPIIVIGGSYGGMLAAWFRLKYPHIALGALASSAPLLYF 220
>At5g22860.1 68418.m02672 serine carboxypeptidase S28 family protein
contains Pfam profile: PF05577 serine carboxypeptidase
S28
Length = 502
Score = 105 bits (253), Expect = 1e-23
Identities = 50/107 (46%), Positives = 74/107 (69%), Gaps = 3/107 (2%)
Frame = +3
Query: 180 GFMWEIAAEYQAKIVFAEHRYYGESKPFGN--KSLEK-EYIGYLTSAQALADYADLINYL 350
GF+ + A +V+ EHRYYGE+ PFG+ ++L+ +GYL +AQALADYA ++ ++
Sbjct: 114 GFLRDNGPRLNALLVYIEHRYYGETMPFGSAEEALKNASTLGYLNAAQALADYAAILLHV 173
Query: 351 QKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMF 491
++ P+I GGSYGGMLAA+ R+KYPH+ GA+A+SAP+ F
Sbjct: 174 KEKYSTNHSPIIVIGGSYGGMLAAWFRLKYPHIALGALASSAPLLYF 220
>At4g36195.1 68417.m05150 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 488
Score = 79.4 bits (187), Expect = 1e-15
Identities = 45/104 (43%), Positives = 62/104 (59%), Gaps = 8/104 (7%)
Frame = +3
Query: 195 IAAEYQAKIVFAEHRYYGESKPFGNKSLEKEYIGYLTSAQALADYADLINYLQKDEIKPR 374
+A ++ A IV EHRYYG+S PF KSL E + YL+S QAL D A Y Q D + +
Sbjct: 104 LAKKFDAGIVSLEHRYYGKSSPF--KSLATENLKYLSSKQALFDLAAFRQYYQ-DSLNVK 160
Query: 375 Y--------PVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPI 482
+ P FG SY G L+A+ R+K+PHL G++A+SA +
Sbjct: 161 FNRSGDVENPWFFFGASYSGALSAWFRLKFPHLTCGSLASSAVV 204
>At4g36190.1 68417.m05149 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 493
Score = 78.2 bits (184), Expect = 3e-15
Identities = 44/104 (42%), Positives = 63/104 (60%), Gaps = 8/104 (7%)
Frame = +3
Query: 195 IAAEYQAKIVFAEHRYYGESKPFGNKSLEKEYIGYLTSAQALADYADLINYLQKDEIKPR 374
+A ++ A IV EHRYYG+S PF KSL + + YL+S QAL+D A Y Q D + +
Sbjct: 104 LAKKFDAGIVSLEHRYYGKSSPF--KSLATKNLKYLSSKQALSDLATFRQYYQ-DSLNVK 160
Query: 375 Y--------PVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPI 482
+ P FG SY G L+A+ R+K+PHL G++A+SA +
Sbjct: 161 FNRSSNVENPWFFFGVSYSGALSAWFRLKFPHLTCGSLASSAVV 204
>At2g18080.1 68415.m02102 serine carboxypeptidase S28 family protein
similar to SP|Q9NQE7 Thymus-specific serine protease
precursor (EC 3.4.-.-) {Homo sapiens}; contains Pfam
profile PF05577: Serine carboxypeptidase S28
Length = 365
Score = 41.9 bits (94), Expect = 3e-04
Identities = 17/35 (48%), Positives = 25/35 (71%)
Frame = +3
Query: 378 PVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPI 482
P FG SY G L+A+ R+K+PHL G++A+SA +
Sbjct: 47 PWFFFGISYSGALSAWFRLKFPHLTCGSLASSAVV 81
>At3g28680.1 68416.m03579 prolylcarboxypeptidase-related weak
similarity to SP|P42785| Lysosomal Pro-X
carboxypeptidase precursor (EC 3.4.16.2)
(Prolylcarboxypeptidase) (PRCP) (Proline
carboxypeptidase) {Homo sapiens}
Length = 199
Score = 37.5 bits (83), Expect = 0.005
Identities = 15/34 (44%), Positives = 25/34 (73%)
Frame = +3
Query: 390 FGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMF 491
F G+ +LAA+ ++KYP++ GA+A+SAP+ F
Sbjct: 16 FHGAVHKVLAAWFKLKYPYIALGALASSAPLLYF 49
>At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family protein
low similarity to SP|Q40708 PIR7A protein {Oryza
sativa}, polyneuridine aldehyde esterase GI:6651393 from
[Rauvolfia serpentina], ethylene-induced esterase
[Citrus sinensis] GI:14279437; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 444
Score = 31.9 bits (69), Expect = 0.27
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Frame = +3
Query: 315 ALADYAD-LINYLQKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASA 476
+LA Y L+++ D +KP VI G +GG +Y YP +A AI SA
Sbjct: 235 SLAQYVKPLLHFF--DTLKPTEKVILVGHDFGGACMSYAMEMYPSKIAKAIFISA 287
>At3g13000.2 68416.m01620 expressed protein contains Pfam profile
PF04784: Protein of unknown function, DUF547
Length = 582
Score = 31.5 bits (68), Expect = 0.36
Identities = 17/46 (36%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = -1
Query: 272 LVTKRLGLSIISVLCENNLGLIFSRYL-PHEAGVIGKCFYLTRKSV 138
L+ K L S++ ++NLG+ SRYL PH+A + +C R+S+
Sbjct: 510 LLPKMLHCYAKSLVEDSNLGVWISRYLPPHQAAFVEQCISQRRQSL 555
>At3g13000.1 68416.m01619 expressed protein contains Pfam profile
PF04784: Protein of unknown function, DUF547
Length = 553
Score = 31.5 bits (68), Expect = 0.36
Identities = 17/46 (36%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = -1
Query: 272 LVTKRLGLSIISVLCENNLGLIFSRYL-PHEAGVIGKCFYLTRKSV 138
L+ K L S++ ++NLG+ SRYL PH+A + +C R+S+
Sbjct: 481 LLPKMLHCYAKSLVEDSNLGVWISRYLPPHQAAFVEQCISQRRQSL 526
>At1g26360.1 68414.m03216 hydrolase, alpha/beta fold family protein
similar to SP|Q40708 PIR7A protein {Oryza sativa},
ethylene-induced esterase [Citrus sinensis] GI:14279437,
polyneuridine aldehyde esterase [Rauvolfia serpentina]
GI:6651393; contains Pfam profile PF00561: alpha/beta
hydrolase fold
Length = 444
Score = 29.1 bits (62), Expect = 1.9
Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +3
Query: 315 ALADYAD-LINYLQKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMF 491
+LA Y+ L+++ + +KP VI G +GG +Y +P +A A+ SA + +
Sbjct: 237 SLAHYSKPLLHFFES--LKPTEKVILVGHDFGGACMSYAMEMFPTKIAKAVFISAAM-LA 293
Query: 492 PGMTKCDL 515
G + DL
Sbjct: 294 NGQSTLDL 301
>At5g24260.1 68418.m02854 prolyl oligopeptidase family protein
similar to dipeptidyl peptidase IV [Stenotrophomonas
maltophilia] GI:1753197; contains Pfam profiles PF00326:
prolyl oligopeptidase family, PF00930: Dipeptidyl
peptidase IV (DPP IV) N-terminal region
Length = 746
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = +3
Query: 390 FGGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMFPG 497
+G SYGG L+A + +YP + A+ + AP+ + G
Sbjct: 612 YGWSYGGYLSATLLTRYPEIFNCAV-SGAPVTSWDG 646
>At2g18360.1 68415.m02139 hydrolase, alpha/beta fold family protein
low similarity to SP|P24640|LIP3_MORSP Lipase 3
precursor (EC 3.1.1.3) (Triacylglycerol lipase)
{Moraxella sp}; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 313
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/41 (31%), Positives = 24/41 (58%)
Frame = +3
Query: 393 GGSYGGMLAAYIRIKYPHLVAGAIAASAPIHMFPGMTKCDL 515
G SYGGM+A I +YP +V + + + + M +++ +L
Sbjct: 135 GFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNL 175
>At4g15370.1 68417.m02349 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase [gi:6650208]
[PMID:11247608]
Length = 759
Score = 27.9 bits (59), Expect = 4.4
Identities = 23/80 (28%), Positives = 32/80 (40%), Gaps = 1/80 (1%)
Frame = +3
Query: 270 KSLEKEYIGYLTSAQALADYADLINYLQKDEIKPRYPVIAFGGSYGGMLAAYIRIKYPHL 449
+S+ E+IG + L D D + YLQ D A G + A + +Y
Sbjct: 506 ESMSSEFIGKKMDVEKLYDAVDFLLYLQSDNGGITAWQPADGKLVEFIEDAVVEHEYVEC 565
Query: 450 VAGAIAASAPIH-MFPGMTK 506
AI A A + FPG K
Sbjct: 566 TGSAIVALAQFNKQFPGYKK 585
>At1g17440.2 68414.m02133 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 27.9 bits (59), Expect = 4.4
Identities = 18/71 (25%), Positives = 33/71 (46%)
Frame = +1
Query: 163 HLPITPASCGR*RLNIKPRLFSQSTDIMESPSLLVTSPWKKSTLGTSRQPRHWLTMPTSS 342
HLP+ + G ++NI+P+L + S + ++ S + + G Q + M S
Sbjct: 240 HLPMLNGAAGAAQMNIQPQLLAASP--RQKSGMVQGSQFHPGSSGQQLQGMQAMGMMGSL 297
Query: 343 ITFKKMKSNPA 375
+M+ NPA
Sbjct: 298 NLTSQMRGNPA 308
>At1g17440.1 68414.m02132 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 27.9 bits (59), Expect = 4.4
Identities = 18/71 (25%), Positives = 33/71 (46%)
Frame = +1
Query: 163 HLPITPASCGR*RLNIKPRLFSQSTDIMESPSLLVTSPWKKSTLGTSRQPRHWLTMPTSS 342
HLP+ + G ++NI+P+L + S + ++ S + + G Q + M S
Sbjct: 240 HLPMLNGAAGAAQMNIQPQLLAASP--RQKSGMVQGSQFHPGSSGQQLQGMQAMGMMGSL 297
Query: 343 ITFKKMKSNPA 375
+M+ NPA
Sbjct: 298 NLTSQMRGNPA 308
>At2g14260.2 68415.m01594 proline iminopeptidase identical to
GP:1710151:U72711
Length = 329
Score = 27.5 bits (58), Expect = 5.8
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = +3
Query: 333 DLINYLQKDEIKPRYPV-IAFGGSYGGMLAAYIRIKYPHLVAGAI 464
DL+N ++K + P + FGGS+G LA +P V G +
Sbjct: 98 DLVNDIEKLREHLKIPEWLVFGGSWGSTLALAYSQSHPDKVTGLV 142
>At2g14260.1 68415.m01595 proline iminopeptidase identical to
GP:1710151:U72711
Length = 380
Score = 27.5 bits (58), Expect = 5.8
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = +3
Query: 333 DLINYLQKDEIKPRYPV-IAFGGSYGGMLAAYIRIKYPHLVAGAI 464
DL+N ++K + P + FGGS+G LA +P V G +
Sbjct: 149 DLVNDIEKLREHLKIPEWLVFGGSWGSTLALAYSQSHPDKVTGLV 193
>At4g24480.1 68417.m03509 serine/threonine protein kinase, putative
similar to serine/threonine-protein kinase CTR1
[Arabidopsis thaliana] SWISS-PROT:Q05609
Length = 963
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +1
Query: 208 IKPRLFSQSTDIMESPSLLVTSPWKKSTLGTSRQP 312
+KP FS S+D E LL++SP + L + P
Sbjct: 122 VKPTRFSMSSDADEERELLLSSPADPAPLPATSSP 156
>At1g67510.1 68414.m07690 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 719
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/46 (36%), Positives = 24/46 (52%)
Frame = -3
Query: 336 GRHSQPVPGLT*GTQCTLFPGTCYQKAWTLHNICALRKQSWLDIQP 199
GR+ QP P LT T+ + G A+ LH C+ RK D++P
Sbjct: 497 GRNGQPSPSLTWSTRIKIAKGAARGLAY-LHE-CSPRKLVHGDVKP 540
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,916,698
Number of Sequences: 28952
Number of extensions: 262490
Number of successful extensions: 737
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 709
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 729
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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