BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS306E01f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g06700.1 68414.m00712 serine/threonine protein kinase, putati... 31 0.47 At3g07600.1 68416.m00910 heavy-metal-associated domain-containin... 30 1.1 At4g33390.1 68417.m04746 hypothetical protein contains Pfam prof... 29 1.4 At2g04270.1 68415.m00416 glycoside hydrolase starch-binding doma... 29 1.9 At2g32320.1 68415.m03951 expressed protein contains Pfam profil... 29 2.5 At2g30740.1 68415.m03749 serine/threonine protein kinase, putati... 29 2.5 At3g55110.1 68416.m06120 ABC transporter family protein ATP-bind... 28 3.3 At3g47910.1 68416.m05224 expressed protein low similarity to non... 28 3.3 At5g53930.1 68418.m06710 expressed protein 28 4.4 At5g54890.1 68418.m06837 expressed protein 27 5.8 At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 27 5.8 At5g05570.1 68418.m00605 transducin family protein / WD-40 repea... 27 5.8 At5g57040.1 68418.m07120 lactoylglutathione lyase family protein... 27 7.7 At2g26920.1 68415.m03229 ubiquitin-associated (UBA)/TS-N domain-... 27 7.7 At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 27 7.7 >At1g06700.1 68414.m00712 serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)[Lycopersicon esculentum] gi|3668069|gb|AAC61805 Length = 361 Score = 31.1 bits (67), Expect = 0.47 Identities = 19/81 (23%), Positives = 35/81 (43%) Frame = +1 Query: 238 QKTKPERKMSEPEPEQKAELISQQWQFSVTVPDINPIKEKVFVTGNTPELGEWDYTKIIM 417 Q++ K S+P P K E+ + V ++ +KEK G+ +GE Y ++ Sbjct: 25 QQSDANHKNSKPAPVAKHEVKKEALPIEVPPLSLDEVKEKTENFGSKALIGEGSYGRVYY 84 Query: 418 MDYDEASNLWLKTLTIPNTCE 480 ++ + LK L + E Sbjct: 85 ATLNDGVAVALKKLDVAPEAE 105 >At3g07600.1 68416.m00910 heavy-metal-associated domain-containing protein identical to residues 23 to 179 of farnesylated protein ATFP4 (putative metal-binding protein) GB:AAD09508 [Arabidopsis thaliana]; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 157 Score = 29.9 bits (64), Expect = 1.1 Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 2/32 (6%) Frame = +1 Query: 223 EERKIQKTKPERKMSEP--EPEQKAELISQQW 312 EE+K ++ KPE K EP +P QK E Q W Sbjct: 91 EEKKPEEKKPEEKKPEPCCQPWQKPEPCYQPW 122 Score = 27.1 bits (57), Expect = 7.7 Identities = 15/31 (48%), Positives = 20/31 (64%) Frame = +1 Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQQWQ 315 EE+K ++ KPE K +PE E+K E Q WQ Sbjct: 86 EEKKPEEKKPEEK--KPE-EKKPEPCCQPWQ 113 >At4g33390.1 68417.m04746 hypothetical protein contains Pfam profile PF05701: Plant protein of unknown function (DUF827) Length = 779 Score = 29.5 bits (63), Expect = 1.4 Identities = 16/63 (25%), Positives = 30/63 (47%), Gaps = 3/63 (4%) Frame = +1 Query: 178 ANNGKINIMQ---RWFFLEERKIQKTKPERKMSEPEPEQKAELISQQWQFSVTVPDINPI 348 A GK+ + Q +W + E+K + K + E++AE + +P +NP+ Sbjct: 699 AKEGKLGVEQELRKWREVSEKKRKNGSSHGKSIQGSKEKEAETSVSNETETNPIPQVNPV 758 Query: 349 KEK 357 K+K Sbjct: 759 KKK 761 >At2g04270.1 68415.m00416 glycoside hydrolase starch-binding domain-containing protein contains Pfam profile: PF00686 starch binding domain Length = 996 Score = 29.1 bits (62), Expect = 1.9 Identities = 15/50 (30%), Positives = 22/50 (44%) Frame = +1 Query: 352 EKVFVTGNTPELGEWDYTKIIMMDYDEASNLWLKTLTIPNTCEVLYRYAI 501 E ++VTG+ LG W+ I M E N W + I + Y Y + Sbjct: 91 EHLYVTGDPSTLGSWEPDCAISMYPTENDNEWEAKVKIASGVNFRYNYLL 140 >At2g32320.1 68415.m03951 expressed protein contains Pfam profile: PF04446 family of unknown function (DUF549) Length = 243 Score = 28.7 bits (61), Expect = 2.5 Identities = 15/36 (41%), Positives = 24/36 (66%), Gaps = 1/36 (2%) Frame = -1 Query: 194 ILP-LFAICHCLFNTKVLFTDTAF*YFIVLNLHIIC 90 +LP LF CLF TKV+F+ +F YF+ + +++C Sbjct: 200 MLPVLFRQGSCLFKTKVIFSIISFFYFLA-SEYLLC 234 >At2g30740.1 68415.m03749 serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)[Lycopersicon esculentum] gi|3668069|gb|AAC61805 Length = 366 Score = 28.7 bits (61), Expect = 2.5 Identities = 17/81 (20%), Positives = 34/81 (41%) Frame = +1 Query: 238 QKTKPERKMSEPEPEQKAELISQQWQFSVTVPDINPIKEKVFVTGNTPELGEWDYTKIIM 417 Q ++ +K +P+ K E + V ++ +KEK G+ +GE Y ++ Sbjct: 28 QNSEANQKNQKPQAVVKPEAQKEALPIEVPPLSVDEVKEKTDNFGSKSLIGEGSYGRVYY 87 Query: 418 MDYDEASNLWLKTLTIPNTCE 480 ++ + LK L + E Sbjct: 88 ATLNDGKAVALKKLDVAPEAE 108 >At3g55110.1 68416.m06120 ABC transporter family protein ATP-binding cassette-sub-family G-member 2, Mus musculus, EMBL:AF140218 Length = 708 Score = 28.3 bits (60), Expect = 3.3 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = -1 Query: 359 TFSLMGFISGTVTENCHCWEISSAFCSGSGSDIFLSGLVFCIFLS 225 TF +G G + +C I +AF SGS F+SGL+ + +S Sbjct: 519 TFWTVGLSGGLESFFYYCLIIYAAFWSGSSIVTFISGLIPNVMMS 563 >At3g47910.1 68416.m05224 expressed protein low similarity to nonmuscle myosin heavy chain (NMHC) [Homo sapiens] GI:189036; contains Pfam profiles PF04780: Protein of unknown function (DUF629), PF04781: Protein of unknown function (DUF627) Length = 1290 Score = 28.3 bits (60), Expect = 3.3 Identities = 14/37 (37%), Positives = 24/37 (64%) Frame = +1 Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQQWQFSVTVP 333 EERK++KT ++ E E ++K + QQ ++S +VP Sbjct: 1026 EERKLEKTLEYQRRIEDEAKEK-HMAEQQKKYSSSVP 1061 >At5g53930.1 68418.m06710 expressed protein Length = 529 Score = 27.9 bits (59), Expect = 4.4 Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 1/55 (1%) Frame = +1 Query: 205 QRWFFLEERKIQKTKPERKMSEPEPEQKAELISQQWQ-FSVTVPDINPIKEKVFV 366 +RW R++ K K R S E E ++E + WQ +P+ NP + K V Sbjct: 143 KRWSRDRGRRLGKVKDSRSRSRDELEGESEEPDECWQGEDEVIPEKNPRRLKSIV 197 >At5g54890.1 68418.m06837 expressed protein Length = 358 Score = 27.5 bits (58), Expect = 5.8 Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 5/47 (10%) Frame = +1 Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQ-----QWQFSVTVPDINPI 348 +E+K QKTK + + SE + K +IS ++ +S T P+I PI Sbjct: 49 KEKKKQKTKKQDQSSELVNDLKIPVISDLPFDFRYSYSETNPEIEPI 95 >At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nicotiana tabacum, EMBL:AB009883 Length = 1008 Score = 27.5 bits (58), Expect = 5.8 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +1 Query: 238 QKTKPERKMSEPEPEQKAELISQQ 309 + TK ERK PE ++KAE + ++ Sbjct: 784 ESTKKERKRKNPESKKKAEAVEEE 807 >At5g05570.1 68418.m00605 transducin family protein / WD-40 repeat family protein similar to unknown protein (pir||T04661); contains Pfam PF00400: WD domain, G-beta repeat (4 copies, 2 weak)|8683726|gb|AV524198.1|AV524198 Length = 1124 Score = 27.5 bits (58), Expect = 5.8 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = -3 Query: 507 ETYGVTI*NFTSIWYC*SFKPKIGSLVIIHH 415 E G+ I ++W C + +GSL++ HH Sbjct: 577 EKKGLAIVTTLTLWICYALSHAVGSLLVAHH 607 >At5g57040.1 68418.m07120 lactoylglutathione lyase family protein / glyoxalase I family protein contains Pfam PF00903: glyoxalase family protein Length = 197 Score = 27.1 bits (57), Expect = 7.7 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +2 Query: 47 NNKLSFNFVIKNNVGKLCVNLER 115 NNK + V ++VG LC NLER Sbjct: 69 NNKTDYGVVGVHHVGLLCENLER 91 >At2g26920.1 68415.m03229 ubiquitin-associated (UBA)/TS-N domain-containing protein contains Pfam profile PF00627: UBA/TS-N domain Length = 646 Score = 27.1 bits (57), Expect = 7.7 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +1 Query: 247 KPERKMSEPEPEQKAELISQQW 312 +PE K+ P+P+Q A L ++W Sbjct: 334 RPELKLDWPKPQQSAALADKRW 355 >At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein similar to SEC14-like protein 2 (Alpha-tocopherol associated protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos taurus}; similar to GI:807956 from [Saccharomyces cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 573 Score = 27.1 bits (57), Expect = 7.7 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +1 Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQQ 309 EE K + P SE +PE+KAE+ +++ Sbjct: 164 EEEKSSEAAPVETKSEEKPEEKAEVTTEK 192 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,885,162 Number of Sequences: 28952 Number of extensions: 216944 Number of successful extensions: 603 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 580 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 602 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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