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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS306E01f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g06700.1 68414.m00712 serine/threonine protein kinase, putati...    31   0.47 
At3g07600.1 68416.m00910 heavy-metal-associated domain-containin...    30   1.1  
At4g33390.1 68417.m04746 hypothetical protein contains Pfam prof...    29   1.4  
At2g04270.1 68415.m00416 glycoside hydrolase starch-binding doma...    29   1.9  
At2g32320.1 68415.m03951 expressed protein  contains Pfam profil...    29   2.5  
At2g30740.1 68415.m03749 serine/threonine protein kinase, putati...    29   2.5  
At3g55110.1 68416.m06120 ABC transporter family protein ATP-bind...    28   3.3  
At3g47910.1 68416.m05224 expressed protein low similarity to non...    28   3.3  
At5g53930.1 68418.m06710 expressed protein                             28   4.4  
At5g54890.1 68418.m06837 expressed protein                             27   5.8  
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic...    27   5.8  
At5g05570.1 68418.m00605 transducin family protein / WD-40 repea...    27   5.8  
At5g57040.1 68418.m07120 lactoylglutathione lyase family protein...    27   7.7  
At2g26920.1 68415.m03229 ubiquitin-associated (UBA)/TS-N domain-...    27   7.7  
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /...    27   7.7  

>At1g06700.1 68414.m00712 serine/threonine protein kinase, putative
           similar to Pto kinase interactor 1 (Pti1)[Lycopersicon
           esculentum] gi|3668069|gb|AAC61805
          Length = 361

 Score = 31.1 bits (67), Expect = 0.47
 Identities = 19/81 (23%), Positives = 35/81 (43%)
 Frame = +1

Query: 238 QKTKPERKMSEPEPEQKAELISQQWQFSVTVPDINPIKEKVFVTGNTPELGEWDYTKIIM 417
           Q++    K S+P P  K E+  +     V    ++ +KEK    G+   +GE  Y ++  
Sbjct: 25  QQSDANHKNSKPAPVAKHEVKKEALPIEVPPLSLDEVKEKTENFGSKALIGEGSYGRVYY 84

Query: 418 MDYDEASNLWLKTLTIPNTCE 480
              ++   + LK L +    E
Sbjct: 85  ATLNDGVAVALKKLDVAPEAE 105


>At3g07600.1 68416.m00910 heavy-metal-associated domain-containing
           protein identical to residues 23 to 179 of farnesylated
           protein ATFP4 (putative metal-binding protein)
           GB:AAD09508 [Arabidopsis thaliana]; contains Pfam
           profile PF00403: Heavy-metal-associated domain
          Length = 157

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
 Frame = +1

Query: 223 EERKIQKTKPERKMSEP--EPEQKAELISQQW 312
           EE+K ++ KPE K  EP  +P QK E   Q W
Sbjct: 91  EEKKPEEKKPEEKKPEPCCQPWQKPEPCYQPW 122



 Score = 27.1 bits (57), Expect = 7.7
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +1

Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQQWQ 315
           EE+K ++ KPE K  +PE E+K E   Q WQ
Sbjct: 86  EEKKPEEKKPEEK--KPE-EKKPEPCCQPWQ 113


>At4g33390.1 68417.m04746 hypothetical protein contains Pfam profile
           PF05701: Plant protein of unknown function (DUF827)
          Length = 779

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 16/63 (25%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
 Frame = +1

Query: 178 ANNGKINIMQ---RWFFLEERKIQKTKPERKMSEPEPEQKAELISQQWQFSVTVPDINPI 348
           A  GK+ + Q   +W  + E+K +      K  +   E++AE        +  +P +NP+
Sbjct: 699 AKEGKLGVEQELRKWREVSEKKRKNGSSHGKSIQGSKEKEAETSVSNETETNPIPQVNPV 758

Query: 349 KEK 357
           K+K
Sbjct: 759 KKK 761


>At2g04270.1 68415.m00416 glycoside hydrolase starch-binding
           domain-containing protein contains Pfam profile: PF00686
           starch binding domain
          Length = 996

 Score = 29.1 bits (62), Expect = 1.9
 Identities = 15/50 (30%), Positives = 22/50 (44%)
 Frame = +1

Query: 352 EKVFVTGNTPELGEWDYTKIIMMDYDEASNLWLKTLTIPNTCEVLYRYAI 501
           E ++VTG+   LG W+    I M   E  N W   + I +     Y Y +
Sbjct: 91  EHLYVTGDPSTLGSWEPDCAISMYPTENDNEWEAKVKIASGVNFRYNYLL 140


>At2g32320.1 68415.m03951 expressed protein  contains Pfam profile:
           PF04446 family of unknown function (DUF549)
          Length = 243

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 15/36 (41%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
 Frame = -1

Query: 194 ILP-LFAICHCLFNTKVLFTDTAF*YFIVLNLHIIC 90
           +LP LF    CLF TKV+F+  +F YF+  + +++C
Sbjct: 200 MLPVLFRQGSCLFKTKVIFSIISFFYFLA-SEYLLC 234


>At2g30740.1 68415.m03749 serine/threonine protein kinase, putative
           similar to Pto kinase interactor 1 (Pti1)[Lycopersicon
           esculentum] gi|3668069|gb|AAC61805
          Length = 366

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 17/81 (20%), Positives = 34/81 (41%)
 Frame = +1

Query: 238 QKTKPERKMSEPEPEQKAELISQQWQFSVTVPDINPIKEKVFVTGNTPELGEWDYTKIIM 417
           Q ++  +K  +P+   K E   +     V    ++ +KEK    G+   +GE  Y ++  
Sbjct: 28  QNSEANQKNQKPQAVVKPEAQKEALPIEVPPLSVDEVKEKTDNFGSKSLIGEGSYGRVYY 87

Query: 418 MDYDEASNLWLKTLTIPNTCE 480
              ++   + LK L +    E
Sbjct: 88  ATLNDGKAVALKKLDVAPEAE 108


>At3g55110.1 68416.m06120 ABC transporter family protein ATP-binding
           cassette-sub-family G-member 2, Mus musculus,
           EMBL:AF140218
          Length = 708

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 16/45 (35%), Positives = 24/45 (53%)
 Frame = -1

Query: 359 TFSLMGFISGTVTENCHCWEISSAFCSGSGSDIFLSGLVFCIFLS 225
           TF  +G   G  +   +C  I +AF SGS    F+SGL+  + +S
Sbjct: 519 TFWTVGLSGGLESFFYYCLIIYAAFWSGSSIVTFISGLIPNVMMS 563


>At3g47910.1 68416.m05224 expressed protein low similarity to
            nonmuscle myosin heavy chain (NMHC) [Homo sapiens]
            GI:189036; contains Pfam profiles PF04780: Protein of
            unknown function (DUF629), PF04781: Protein of unknown
            function (DUF627)
          Length = 1290

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 14/37 (37%), Positives = 24/37 (64%)
 Frame = +1

Query: 223  EERKIQKTKPERKMSEPEPEQKAELISQQWQFSVTVP 333
            EERK++KT   ++  E E ++K  +  QQ ++S +VP
Sbjct: 1026 EERKLEKTLEYQRRIEDEAKEK-HMAEQQKKYSSSVP 1061


>At5g53930.1 68418.m06710 expressed protein
          Length = 529

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = +1

Query: 205 QRWFFLEERKIQKTKPERKMSEPEPEQKAELISQQWQ-FSVTVPDINPIKEKVFV 366
           +RW     R++ K K  R  S  E E ++E   + WQ     +P+ NP + K  V
Sbjct: 143 KRWSRDRGRRLGKVKDSRSRSRDELEGESEEPDECWQGEDEVIPEKNPRRLKSIV 197


>At5g54890.1 68418.m06837 expressed protein
          Length = 358

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 5/47 (10%)
 Frame = +1

Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQ-----QWQFSVTVPDINPI 348
           +E+K QKTK + + SE   + K  +IS      ++ +S T P+I PI
Sbjct: 49  KEKKKQKTKKQDQSSELVNDLKIPVISDLPFDFRYSYSETNPEIEPI 95


>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
           Nicotiana tabacum, EMBL:AB009883
          Length = 1008

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +1

Query: 238 QKTKPERKMSEPEPEQKAELISQQ 309
           + TK ERK   PE ++KAE + ++
Sbjct: 784 ESTKKERKRKNPESKKKAEAVEEE 807


>At5g05570.1 68418.m00605 transducin family protein / WD-40 repeat
           family protein similar to unknown protein (pir||T04661);
           contains Pfam PF00400: WD domain, G-beta repeat (4
           copies, 2 weak)|8683726|gb|AV524198.1|AV524198
          Length = 1124

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = -3

Query: 507 ETYGVTI*NFTSIWYC*SFKPKIGSLVIIHH 415
           E  G+ I    ++W C +    +GSL++ HH
Sbjct: 577 EKKGLAIVTTLTLWICYALSHAVGSLLVAHH 607


>At5g57040.1 68418.m07120 lactoylglutathione lyase family protein /
           glyoxalase I family protein contains Pfam PF00903:
           glyoxalase family protein
          Length = 197

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = +2

Query: 47  NNKLSFNFVIKNNVGKLCVNLER 115
           NNK  +  V  ++VG LC NLER
Sbjct: 69  NNKTDYGVVGVHHVGLLCENLER 91


>At2g26920.1 68415.m03229 ubiquitin-associated (UBA)/TS-N
           domain-containing protein contains Pfam profile PF00627:
           UBA/TS-N domain
          Length = 646

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = +1

Query: 247 KPERKMSEPEPEQKAELISQQW 312
           +PE K+  P+P+Q A L  ++W
Sbjct: 334 RPELKLDWPKPQQSAALADKRW 355


>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
           phosphoglyceride transfer family protein similar to
           SEC14-like protein 2 (Alpha-tocopherol associated
           protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
           taurus}; similar to GI:807956 from [Saccharomyces
           cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
           contains Pfam PF03765 : CRAL/TRIO, N-terminus
          Length = 573

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +1

Query: 223 EERKIQKTKPERKMSEPEPEQKAELISQQ 309
           EE K  +  P    SE +PE+KAE+ +++
Sbjct: 164 EEEKSSEAAPVETKSEEKPEEKAEVTTEK 192


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,885,162
Number of Sequences: 28952
Number of extensions: 216944
Number of successful extensions: 603
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 602
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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