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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS306D12f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g41910.1 68418.m05102 RNA polymerase II mediator complex prot...    97   5e-21
At1g26665.2 68414.m03248 expressed protein                             96   1e-20
At1g26665.1 68414.m03247 expressed protein                             96   1e-20
At2g07725.1 68415.m00975 60S ribosomal protein L5 (RPL5) identic...    31   0.36 
At5g38195.1 68418.m04604 protease inhibitor/seed storage/lipid t...    29   2.5  
At2g44410.1 68415.m05523 expressed protein                             28   3.3  
At3g26115.2 68416.m03254 expressed protein                             28   4.4  
At3g26115.1 68416.m03253 expressed protein                             28   4.4  
At1g56040.1 68414.m06434 U-box domain-containing protein contain...    28   4.4  
At5g10060.1 68418.m01165 expressed protein                             27   5.8  

>At5g41910.1 68418.m05102 RNA polymerase II mediator complex
           protein-related similar to SP|P87310 RNA polymerase II
           mediator complex protein nut2 {Schizosaccharomyces
           pombe}
          Length = 186

 Score = 97.5 bits (232), Expect = 5e-21
 Identities = 49/135 (36%), Positives = 80/135 (59%), Gaps = 1/135 (0%)
 Frame = +1

Query: 115 ISIKNSMSSPLENLETQLEMFIENVRQIRIIVSDFQPQGQSVLNQKIQSLVTGLQEIDKL 294
           I+  +  +  L  +    +  +E + Q+++ VS F P  Q  L Q++ SLV  L  + KL
Sbjct: 25  IATVDDSNEKLNQIINSNQKILELLHQLKLTVSSFTPASQLHLLQRLNSLVMELDNMAKL 84

Query: 295 KSQVHDIHVPTEVFDYIDQGRNPQLYTKDCIDK-ALAKNEEVKGKIDSYKRFKTHLLSEL 471
             + + I VP EV + ID G+NP  +TKD ++K  +AKN+  KGK D++K  + HLL EL
Sbjct: 85  SDKCN-IQVPIEVLNLIDDGKNPDEFTKDVLNKNCIAKNQVTKGKSDAFKGLRKHLLEEL 143

Query: 472 SKTFPNEITKYKALR 516
            + FP+E+ +Y+ +R
Sbjct: 144 EQAFPDEVDRYRDIR 158


>At1g26665.2 68414.m03248 expressed protein
          Length = 189

 Score = 95.9 bits (228), Expect = 1e-20
 Identities = 44/134 (32%), Positives = 78/134 (58%)
 Frame = +1

Query: 115 ISIKNSMSSPLENLETQLEMFIENVRQIRIIVSDFQPQGQSVLNQKIQSLVTGLQEIDKL 294
           +++ +     L  +   +E  +  + Q+ + V+ F P  Q  L Q++ SLV  L  + KL
Sbjct: 29  VTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKL 88

Query: 295 KSQVHDIHVPTEVFDYIDQGRNPQLYTKDCIDKALAKNEEVKGKIDSYKRFKTHLLSELS 474
            S+  +I +P EV + ID G+NP  +TKD ++  +A+N+  KGK D++K  + H+L EL 
Sbjct: 89  -SEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELE 147

Query: 475 KTFPNEITKYKALR 516
           + FP+E+  Y+ +R
Sbjct: 148 QNFPDEVDMYREIR 161


>At1g26665.1 68414.m03247 expressed protein
          Length = 189

 Score = 95.9 bits (228), Expect = 1e-20
 Identities = 44/134 (32%), Positives = 78/134 (58%)
 Frame = +1

Query: 115 ISIKNSMSSPLENLETQLEMFIENVRQIRIIVSDFQPQGQSVLNQKIQSLVTGLQEIDKL 294
           +++ +     L  +   +E  +  + Q+ + V+ F P  Q  L Q++ SLV  L  + KL
Sbjct: 29  VTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKL 88

Query: 295 KSQVHDIHVPTEVFDYIDQGRNPQLYTKDCIDKALAKNEEVKGKIDSYKRFKTHLLSELS 474
            S+  +I +P EV + ID G+NP  +TKD ++  +A+N+  KGK D++K  + H+L EL 
Sbjct: 89  -SEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELE 147

Query: 475 KTFPNEITKYKALR 516
           + FP+E+  Y+ +R
Sbjct: 148 QNFPDEVDMYREIR 161


>At2g07725.1 68415.m00975 60S ribosomal protein L5 (RPL5) identical
           to SP|P42793 Mitochondrial 60S ribosomal protein L5
           {Arabidopsis thaliana}
          Length = 185

 Score = 31.5 bits (68), Expect = 0.36
 Identities = 17/63 (26%), Positives = 33/63 (52%)
 Frame = -2

Query: 361 GYGLDQYNQRLLLVHGYHELVTSICQSPAIQSLRTEFFDSVHFAPEVENH*RLYEFVSHF 182
           G+G+  ++ R+  V    +    I ++    S+ TEF +   F+PE+E+H  ++E +  F
Sbjct: 99  GHGMSNFSVRISTVMSLLDFPVEIRKNSIQFSMETEFCE---FSPELEDHFEIFEHIRGF 155

Query: 181 Q*T 173
             T
Sbjct: 156 NVT 158


>At5g38195.1 68418.m04604 protease inhibitor/seed storage/lipid
           transfer protein (LTP) family protein contains Pfam
           protease inhibitor/seed storage/LTP family domain
           PF00234
          Length = 95

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 15/48 (31%), Positives = 25/48 (52%)
 Frame = +1

Query: 292 LKSQVHDIHVPTEVFDYIDQGRNPQLYTKDCIDKALAKNEEVKGKIDS 435
           +KS+V    +PTE+   +         TKDC DK + + E + G I++
Sbjct: 22  IKSEVSSSCIPTELMPCLPAMTTGGQPTKDCCDKLIEQKECLCGYINN 69


>At2g44410.1 68415.m05523 expressed protein 
          Length = 413

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 16/64 (25%), Positives = 31/64 (48%)
 Frame = +1

Query: 121 IKNSMSSPLENLETQLEMFIENVRQIRIIVSDFQPQGQSVLNQKIQSLVTGLQEIDKLKS 300
           +  ++S  LE LE+  E   E +RQ+ +IVS  +   + +      +LV+  +  D    
Sbjct: 28  LMTALSPMLEELESAQERIQERIRQLEVIVSRIRE--REITTTTTPALVSPNEHRDSTAG 85

Query: 301 QVHD 312
            +H+
Sbjct: 86  VIHE 89


>At3g26115.2 68416.m03254 expressed protein
          Length = 433

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +1

Query: 427 IDSYKRFKTHLLSELSKTFPNEI 495
           + +YKR + HL++E S+ FP  +
Sbjct: 310 LKNYKRHEDHLIAEFSRQFPGSV 332


>At3g26115.1 68416.m03253 expressed protein
          Length = 427

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +1

Query: 427 IDSYKRFKTHLLSELSKTFPNEI 495
           + +YKR + HL++E S+ FP  +
Sbjct: 310 LKNYKRHEDHLIAEFSRQFPGSV 332


>At1g56040.1 68414.m06434 U-box domain-containing protein contains
           Pfam profile PF04564: U-box domain
          Length = 437

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 17/80 (21%), Positives = 48/80 (60%), Gaps = 1/80 (1%)
 Frame = +1

Query: 280 EIDKLKSQVHDIHVPTEVFDYIDQGRNP-QLYTKDCIDKALAKNEEVKGKIDSYKRFKTH 456
           +++ +++++ +  +  E F+   +G    +  ++  ++K   + EEVK K+++Y+R + +
Sbjct: 224 QLETVENEIDNTRLKAEEFERKYEGEMILRRESEIALEKEKKELEEVKLKLETYEREQEN 283

Query: 457 LLSELSKTFPNEITKYKALR 516
           L SE+ +T+ ++  +  +LR
Sbjct: 284 LSSEV-RTWQDKYEQESSLR 302


>At5g10060.1 68418.m01165 expressed protein
          Length = 469

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 22/84 (26%), Positives = 37/84 (44%)
 Frame = +1

Query: 124 KNSMSSPLENLETQLEMFIENVRQIRIIVSDFQPQGQSVLNQKIQSLVTGLQEIDKLKSQ 303
           + S++  LE  E  L   IE ++ +       Q    S++NQ   +L     E+D LK+Q
Sbjct: 220 RKSLAKELEEEEYLLRQCIEKLKSV-------QGSRSSLVNQLKDALREQESELDNLKAQ 272

Query: 304 VHDIHVPTEVFDYIDQGRNPQLYT 375
           +      TE    + +  N + YT
Sbjct: 273 IQVAKEQTEEAQNMQKRLNDEDYT 296


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,542,181
Number of Sequences: 28952
Number of extensions: 176513
Number of successful extensions: 534
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 518
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 530
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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